본 연구는 실내-외 우리가 있는 개방형 흑염소사에서 우리나라 5개월령 재래 흑염소를 대상으로 서열과 일주기 리듬이 하루 24시간 동안의 행동에 미치는 영향과 서열과 사료급여시간이 사료급여 후 30분 및 60분 동안의 행동에 미치는 영향을 조사하기 위해 수행하였다. 재래 흑염소의 행동은 CCTV를 사용해 녹화한 후 이들을 1분 단위로 측정하였고, 총 10개의 행동으로 분류하였다. 서열은 적대행동에 유의하게 영향을 미쳤으나 (p<0.05), 다른 행동에는 영향을 미치지 않았다. 일주기 리듬은 응립(p<0.05), 이동(p<0.01), 사료섭취(p<0.001), 적대행동(p<0.001), 비적대행동 (p<0.001), 사료탐색(p<0.05), 음수(p<0.001) 및 기타 행동(p<0.001)에 유의하게 영향을 미치는 반면 휴식(p>0.05)과 몸단장(p>0.05)에는 영향을 미치지 않았다. 서열과 일주기 리듬의 상호작용효과는 적대행동에만 유의하게 영향을 미쳤다(p<0.05). 서열은 사료급여 후 30분 동안 응립(p<0.05)과 사료섭취(p<0.05)에 유의하게 영향을 미쳤지만 다른 행동에는 영향을 미치지 않았다. 사료급여시간은 사료급여 후 60분 동안의 음수(p<0.05)에 유의하게 영향을 미쳤으나, 다른 행동에는 영향을 미치지 않았다. 서열과 사료급여시간의 상호작용효과는 모든 행동에 유의하게 영향을 미치지 않았다(p<0.05). 결론적으로, 서열, 일주기 리듬, 그리고 사료급여시간은 우리나라 5개월령 미성성숙한 재래 흑염소의 행동에 영향을 미치는 것으로 나타났다.

The seroprevalence of major infectious abortion agents was investigated in native Korean goats from Jeonnam province using ELISA. A total of 98 blood samples were collected from goats. Overall, 48 (49.0%), 11 (11.2%), and 1 (1.0%) serum samples tested positive for C. burnetii, T. gondii, and C. abortus, respectively, while none of the samples were positive for B. abortus or N. caninum. Moreover, a PCR assay conducted to detect C. burnetii antigens in goats with a history of abortion revealed 18 (38.3%) of 47 goats were C. burnetii positive by both the ELISA and PCR assay.

Cytokeratin (CK) comprises the intermediate filament cytoskeleton of epithelial cells. Patterns of CK expression can be regarded as a specific marker for epithelial differentiation status. The aim of this study was to identify CK expression on tongues of Korean native goats ranging from 60-day-old fetuses to newborns during prenatal development using immunohistochemistry. The tongues of fetuses were removed from 2- to 4-year-old female Korean native goats by caesarean section performed under general anesthesia. Immunohistochemistry was performed to assess CK expression patterns on developing goat tongues using serial paraffin-embedded sections. Light zones signifying CK immunoreactivity in dorsal lingual epithelia were weakly positive in 60-day-old fetuses. In 90-day-old fetuses, deep areas in dorsal lingual epithelia were strongly positive for CK expression and superficial areas were moderately positive. In 120-day-old fetuses, light zones of lingual epithelia in the vallate papilla were strongly positive for CK expression, whereas ducts of von Ebner’s glands were moderately positive. In neonates, taste buds were positive for CK expression, whereas non-taste epithelial cells and von Ebner’s glands were negative. These findings indicate that goat tongues have different patterns of CK expression during development and provide a morphological basis for studies on the biological mechanism of epithelial differentiation.

The purpose of this study was to identify the composition and organization of lingual tissues underlying the histo-structural change of developing tongue in Korean native goats by light microscopy (LM). Tongues of the fetuses on days 60, 90, 120 and neonate were examined for the morphological development. In the 60-day-old fetuses, the tongue tissues were differentiated into epithelium, lamina propria and muscle layer. Primordia of filiform, conical, lentiform, fungiform and vallate papillae appeared and rudiments of taste bud were observed in the epithelia of the primordia of the gustatory papillae. The dorsal surface of the lingual epithelia showed a weak PAS positive reaction. Collagenous fibers and small blood vessels were shown in the connective tissues. In the 90-day-old fetuses, Von Ebner’s glands were moderately PAS positive while the muscle fibers and connective tissue were strongly positive for PAS. The collagenous fibers increased and came to have a more complex arrangement in the tongue. The muscle fibers were spread out at various directions and developed in striated muscle bundles. In the 120-day-old fetuses, taste buds were observed in the epithelia of the gustatory papillae, and several well-developed tissues visible such as blood vessels, collagenous fibers, muscle fiber bundles and Von Ebner’s glands. In the neonates, many taste buds were found in a transverse section of the vallate papilla. The muscle layers, Von Ebner’s glands, collagenous fibers and blood vessels were more developed than those of the 120-day old fetuses. These findings indicate that goat tongues have a variety of different shapes during prenatal development.

The purpose of this study was to explore the morphologi- cal characteristics of developing lentiform papilla (LP) in Korean native goats by scanning electron microscopy (SAM). Tongues were removed from fetuses on days 90, 120, neonates, and juveniles on days 30, 60, 90, 120, 150, and 180. In prenatal development, the primordia of LP in 90-day-old fetuses were round and spotted on the inner most part of the torus linguae of the tongue. Primordia of LP in 120-day-old fetuses also had a lens-like shape. In neonates, LP displayed similar features as the adult one. In postnatal juveniles on days 30 and 60, LP continually increased in size without much difference in structure compared to that of neonates. By postnatal day 90, detached pieces of keratinized super- ficial epithelia were observed. Microridges and microplicae were well developed on the epithelial surface of LP in 60- to 120-day-old goats. The lengths of LP were 476~514 μm in neonates, 687~962 μm in the weaning period of 60-day- old goats, and 1,068~1,567 μm in the maturing period of 180-day-old goats. These findings indicate that goat LP has different sizes and shapes from those of other species during development.

This study assesses of efficiency of oocyte recovery and in vitro development for during the non breeding season in goat. Thirty-four matured goats, maintained in a pen under natural day length and fed hay ad libitum, were pretreated with progestagen implanted CIDR for 10 days. Superovulation treatment of the goats received twice daily intramuscular injections of a total of 70 mg FSH for 3 days from Day 8 of CIDR. All the gonadotropin treated goats were injected with 10 mg PGF2α on Day 8 and 400～600 IU hCG in the afternoon on Day 10. Oocytes were recovered by follicle aspiration or oviduct flushing at 35 to 40 h after hCG injection through mid-ventral incision. The in vivo matured oocytes were activated by ionomycin (5 min) and 6-DMAP (3.5～4 h). The activated oocytes were cultured in mSOF medium containing 0.8% BSA at 38.5℃ in an atmosphere of 5% CO2, 5% O2, 90% N2 for 7～8 days. There was no significant difference in the mean number of CL and in vivo matured and follicular oocytes recovered. But, quality of I+II grade follicular oocytes was lower (p<0.05) in the prepubertal goat (25.0%) than the adults (52.4%). The same results were also observed in the cleavage and blastocyst rate of activated oocytes. The clavage and blastocyst rate from prepubertal derived oocytes were lower (p<0.05) in the prepubertal goat (54.5%, 23.3%) than the adult goat (86.8%, 46.6%). Considering overall these results, we suggest that maturation of donor goats is a major factor affecting recovered oocytes quality and in vitro development of activated goat oocytes. There was no significant difference in oocyte quality between seasonal treatments.

The aim of this study was to investigate the morphological development pattern of conical papilla (CP) during prenatal and postnatal periods in native Korean goats by scanning electron microscopy. Tongues were removed from fetuses on days 90, 120, and neonates and from juveniles on days 30, 50, 60, 75, 90, 120, 150, and 180 after birth. The lengths of CP were 194~240 μm in neonates, 335~485 μm in the weaning period of 60-day-old goats, and 526~662 μm in the maturing period of 180-day-old goats. The primordia of CP in 90-day-old fetuses, shaped like mountain berry, were sprouted. The CP of 120-day-old fetuses was a cylinder shape with an apex whose center was slightly concave and the margin was sticking up like a low fence. The CP of neonates was empty inside and the secondary papillae were irregularly arranged fence-like structures on it. In 60-day-old goats, CP was an obliquely sectioned cylinder shape compacted in inner surface; the shapes of CP varied in 120-day-old goats, and had a smooth surface. A low elevation of CP was observed in 150- and 180-day-old goats. The microridges, microplicae, and micropits were well developed on the epithelial surface of lingual papillae in 60- to 120-day-old goats. These findings indicate that CP of goats has a variety of sizes and shapes during development.

The aim of this study was to investigate the morphological development of fungiform papillae in native Korean goats by scanning electron microscopy (SEM). Tongues were removed from fetuses on days 120 and 150 of gestation and from juveniles on days 30, 60, 90, 120, 150, and 180 after birth. The sizes of fungiform papillae in the newborn were 225.0-250.0 μm, the weaning period of 60-day-old goats was 271-294 μm and the maturing period of 180-day-old goats was 385-398 μm, respectively. The primordia of fungiform papillae had the typical feature of a mushroom shape in a 120-day-old fetus. In a 150-day-old fetus (neonate), fungiform papillae had features similar to those of the adult form and showed a gradual increase in size with slight morphological changes until 150-day-old goats. Microridges, microplicae, and micropits were well developed on the epithelial surface of lingual papillae from 60 to 150-day-old goats. The results obtained in this study might provide some basic data on the mechanisms that control the morphogenesis and development of the lingual papillae in ruminants.

본 연구는 효율적인 재래 산양의 난포란을 확보하기 위하여 미성숙 산양에 과배란 처리를 실시하여 체내 성숙 난자(배란된 난자) 및 난포란을 회수하여 공란산양의 성숙 여부가 난자의 회수율과 단위 발생란의 체외발달율에 미치는 영향을 조사하였다. 미성숙 산양의 과배란 처리에 의한 체내 성숙 난자의 회수율에 있어서 과배란 처리 후 두당 황체수는 13.16.5개로서 성숙 산양의 8.82.4개와 차이가 없었다(p<0.05). 산양의 두당 회수율은 마성숙 산양이 9.

본 시험은 흑염소의 효율적인 사양관리체계의 확립에 기여하기 위하여, 재래흑염소 10두(male)와 교잡흑염소(재래흑염소호주야생염소 교잡종; 50:50%) 10두(male)를 공시하여, 시판 배합사료와 조사료를 급여하는 관행사료와 TMR 사료를 각각 급여하였을 때, 흑염소의 사료섭취량, 영양소소화율, 질소축적율 및 혈액성상에 미치는 영향을 조사하였으며, 실험기간은 사료 적응기간 14일 예비기간 10일을 거친 후, 14일간의 본 실험 기간을 두어 총 38일

본 연구는 2007년 3월부터 10월까지 충남대학교 부속사육장 및 전북 남원시 운봉읍 소재 가축유전자원시험장에서 수행하였다. 공시시료는 화본과목초(gasses)는 Lolium perenne, Festuca arundinacea, Poa pratensis, Agrostis alba 및 mixed grasses의 5종과, 두과목초(legumes)는 Trifolium pratense, Medicago sative, Trifolium repens, Melilo

본 연구는 섬유질 배합사료에 맥주박 수준을 달리 첨가하여 번식 흑염소의 사료 및 영양소 섭취량, 영양소 소화율 및 질소 축적율에 미치는 영향을 조사하여 번식 흑염소의 사료로서 맥주박의 사료가치를 평가하고자 실시하였다. 공시축은 번식 흑염소 12두를 4처리구(관행사료구, 맥주박 20, 30 및 40% 첨가구)로 나누어 처리구당 3두씩 대사케이지에 완전임의 배치법으로 배치하여 시험을 실시하였으며 그 결과는 다음과 같다. 맥주박 첨가 수준별 섬유질 배합사료의

본 연구는 2000년 7월부터 12월까지 충남대학교 생명과학대학 내 부속 환경조절축사에서 수행하였다. 공시된 혼파유형별 목초는 관행 혼파초지(orchardgrass 50+tall fescue 20+perennial ryegrass 10+ Kentucky bluegrass 10+white clover ), 다초종 혼파초지(orchardgrass 40+tall fescue 20+perennial ryegrass 10+Kentucky bluegrass 10+

본 연구는 재래산양에서 복제 수정란의 생산효율을 향상시키기 위한 기초 자료를 제시하고자 체세포 핵이식을 실시하여 공핵세포의 종류, 핵이식란의 활성화 처리 방법 및 수핵난자의 조건이 체외발달율에 미치는 영향을 조사, 검토하여 핵이식란 생산을 위한 최적의 조건을 규명하고자 실시하였다. 공핵세포의 종류에 따른 핵이식란의 체외발달율은 융합이 이루어진 핵이식란의 활성화 처리 후 분할율은 귀 유래 섬유아세포를 공핵세포로 사용하였을 때가 로서 태아 유래 섬유아세포를