간행물
Journal of Biomedical Research
- 발행기관 충북대학교 동물의학연구소
- 자료유형 학술지
- 간기 계간
- ISSN 1976-7447 (Print)2287-7363 (Online)
- 수록기간 2000 ~ 2015
- 주제분류 의약학 > 수의학 의약학 분류의 다른 간행물
- 십진분류KDC 528DDC 636
권호리스트/논문검색
Vol. 13 No. 4 (2012년 12월) 14건
Original Article
1.
2012.12
구독 인증기관 무료, 개인회원 유료
Vital pulpotomy is a very useful method for disarming of canine tooth, tooth fracture, periodontitis, and malocclusion in veterinary dentistry. Calcium hydroxide is the material commonly used as a liner during vital pulpotomy. This creates a mineralized barrier by stimulating osteoblastic hard tissue repair, arrests the inflammatory response, and soothes dentin. However, the powder or mix type calcium hydroxide materials have many disadvantages due to complicated procedures for use and are hard to handle when vital pulpotomy is followed under general anesthesia in animals. This study was conducted in order to compare the effect of mix and premixed paste type calcium hydroxide as a liner in vital pulpotomy. Six beagle dogs underwent hemisection on the mesial root of the mandibular first molar and vital pulpotomy on the distal root of the first molar. On the distal root of the left and right mandibular first molar, mix type (DYCAL®, Dentsply, USA) and premixed paste type calcium hydroxide (VITAPEX®, Morita, Japan) were used as liners, respectively. Radiological evaluation was performed at immediate, 4, 12, and 20 weeks after vital pulpotomy. According to the results, all teeth had well-formed dentinal bridges, and there were no periradicular lucency, lamina dura loss, or anomalies of the pulp cavity. According to these results, on vital pulpotomy in animals, premixed paste type calcium hydroxide was easy to handle and decreased the anesthesia period due to a more convenient application procedure. A further study of many clinical cases is needed for evaluation of side effects and other problems.
4,000원
2.
2012.12
구독 인증기관 무료, 개인회원 유료
Sung-Ho Kim, Miyoung Yang, Changjong Moon, Jong-Choon Kim, Chun-Sik Bae, Jong-Sik Jang, Young-Hyun Choi, Su-Ryeon Noh
To evaluate the acute to chronic effects of crude oil exposure on hematological and blood biochemical toxicities, Sprague-Dawley rats were given oral doses of 0, 50, or 100 mg/kg BW/day of Iranian heavy crude oil for a period of four weeks. In the acute phase of exposure (one day after four weeks of oil treatment), decreases in weight of thymus, serum level of interferon gamma (IFN-γ), superoxide dismutase (SOD), and catalase activities in liver or kidney, and increase in weight of adrenal gland occurred after oral administration of crude oil. In body weight, histopathological examination, hematological and blood biochemical analyses in the acute phase of exposure, no significant differences were observed among the experimental groups. In the subchronic and chronic phase of exposure (two months and six months after four weeks of oil treatment), the changes of biomarkers were normalized, except the indicators of oxidative stress. Our findings showed that the bioassay on the indicators of oxidative stress is a sensitive method for determining exposure to crude oil in rats.
4,000원
3.
2012.12
구독 인증기관 무료, 개인회원 유료
The aim of this experiment was to count excreted oocysts and to evaluate gross lesions and histopathological changes in broiler chicks infected with Eimeria; E. acervulina (Ea), E. maxima (Em), and E. tenella (Et). Twelve-day-old broiler chicks without infection with Eimeria spp. were used in this experiment. On day 12, the chicks in group Ea (n=3), Em (n=3), and Et (n=3) received oral inoculation with 5 × 10 sporulated oocysts of Ea, Em, and Et per 1 mL of tap water, while control chicks were injected with tap water of equal amount. The prepatent period (PP) for Ea, Em, and Et was four, five, and six days, respectively. To evaluate the progress of the coccidiosis, post-mortem analysis method and histopathological evaluation were performed two days after PP of Ea and Et, and three days after PP of Em at two-day intervals on one bird from each group. Intestinal segments (Ea, duodenum and upper intestine; Em, mid intestine; and, Et, cecum) of all chicks were obtained from the autopsied birds. Among pathological changes of coccidiosis, target parts of small intestine and caecal pouches showed mucosal petechiation and white plaques streaks in the duodenum. Histopathological evidence showed upper small intestinal villus preserved with different phases of parasite (zygotes, oocysts, macro- and micro-gametocytes) after infection with Ea in broiler chicks. Zygotes in the lamina propria and fusion of middle small intestinal villi in broilers infected with Em and invasion of the caecal crypt of oocysts and granulomatous inflammation of caecal submucosa in broilers infected with Et were found.
4,000원
4.
2012.12
구독 인증기관 무료, 개인회원 유료
Young-Kug Choo, Jae-Sung Ryu, Malg-Um Lim, Mi-Ran Hwang, Hyun-Ki Min, Gislain Moussavou, Ji-Su Kim, Sun-Uk Kim, Kisung Ko, Young-Ho Cho, Sang-Yoon Nam, Kyu-Tae Chang
Flavonoids have a range of biological activities, including anti-allergic, anti-inflammatory, anti-microbial, and anti-cancer activities, as demonstrated by in vitro studies. In this study, we investigated whether luteolin can be applied to suppression of lipopolysaccharide (LPS)-stimulated inflammatory responses in murine macrophages. Luteolin was found to reduce nitric oxide (NO) production in LPS-stimulated Raw 264.7 cells. In addition, expression of inducible nitric oxide synthetase (iNOS), cyclooxygenase-2 (COX-2), and pro-inflammatory cytokine tumor necrotic factor-α (TNF-α) at the mRNA and protein levels were decreased. These inhibitory effects were found to be caused by the blockade of nuclear factor kappa-light- chain-enhancer of activated B cells (NF-κB) activation and phosphorylation of mitogen-activated protein (MAP) kinase family, extracellular signal-regulated kinases 1/2 (ERK1/2), c-Jun N-terminal kinase (JNK), and p38 MAP kinase. In addition, pre-treatment with luteolin resulted in reduced ganglioside expression levels and inhibited expression of GT1b in Raw 264.7 cells. On the basis of these observations, we suggest that luteolin has potential as an anti-inflammatory drug candidate, and ganglioside GT1b may play a role in the inflammatory process.
4,000원
5.
2012.12
구독 인증기관 무료, 개인회원 유료
This study was conducted in order to evaluate three different non- invasive blood pressure measurement methods (NIBP) in comparison with direct blood pressure measurements in anesthetized dogs. Ten normal healthy Beagle dogs (mean age; 3.7 ± 1.01 yrs, mean body weight 10.2 ± 1.15 kg) were enrolled in this study. BP was measured using three different methods, i.e., Doppler (DOP), oscillometric, and high density oscillometric (HDO) methods, simultaneously, five times in each subject under anesthesia at three different locations (right, left front legs, and tail). The blood pressure value measured by the Doppler method was the closest to the value measured by the direct method. Although the accuracy and consistency of BP measured by three different non-invasive methods differed according to the method, all methods, except the conventional oscillometric method, were acceptable for monitoring systolic blood pressure in anesthetic dogs. The inter-measurement variations in DOP and HDO were minimal without affecting the consistency of the test results.
4,000원
6.
2012.12
구독 인증기관 무료, 개인회원 유료
Sei Jin Youn, Kyoung Soo Lee, Young Shim Cho, Joung Ho Han, Soon Man Yoon, Hee Bok Chae, Seon Mee Park
Although bombesin (BN) regulates colonic motility, the associated mechanism of action remains unclear. The objective of this study was to investigate the effect of BN on colonic motility using isolated rat colon. An isolated rat colon was perfused with Krebs solution via the superior mesenteric artery. Intraluminal pressure was measured via microtip catheter pressure transducers at both proximal and distal portions of the isolated colon. After a control period, BN was administered intraarterially in concentrations of 13, 26, 130, and 260 pM. After pretreatment with hexamethonium (10-3 M), atropine (10-5 M), or tetrodotoxin (10-6 M), BN was administered at a concentration of 260 pM (proximal colon) or 130 pM (distal colon); intraluminal pressure was then monitored. Changes in motility were expressed as the percentage change of motility index (MI) over the basal period. As a result, BN increased colonic motility and a dose-dependent increase in proximal colonic motility was observed. The stimulant effect of BN was almost completely abolished by atropine and tetrodotoxin at both the proximal and distal colon. However, BN was not inhibited by hexamethonium at both the proximal and distal colon. Therefore, the stimulant action of BN may be mediated by local cholinergic muscarinic receptors.
4,000원
7.
2012.12
구독 인증기관 무료, 개인회원 유료
Human umbilical cord is easy to obtain because it is discarded after birth, so that ethical issues can be avoided. Chondrogenesis studies using MSCs from bone marrow, cord blood, and adipose have indicated that TGFβ3 and BMP6 stimulate chondrogenesis. Therefore, we investigated chondrogenesis of hUC-MSCs on TGFβ3, BMP6, and combination of the two growth factors. We initiated chondrogenesis of cells by application of physical forces to form 3D cell clusters. After initiation, we designated four experimental groups for differentiation of cells, as follows: control, 10 ng/mL TGFβ3, 100 ng/mL BMP6, and the combination of 5 ng/mL TGFβ3 and 50 ng/mL BMP6. For analysis of chondrogenesis, GAG contents, mRNA expression, histological analysis and immunohistochemistry (IHC) were performed. For analysis of GAG contents, GAG assay was performed and RT-PCR was performed for determination of chondrogenic markers. Histological analysis was performed through safranin O, alcian blue, and IHC was performed using collagen type I and II. GAG contents were increased 184% by TGFβ3, 147% by BMP6, and 189% by the combination of TGFβ3 and BMP6, compared to control. The growth factors improved collagen II and aggrecan expression; in particular, TGFβ3 and BMP6 showed a synergistic effect, compared to only TGFβ3 or BMP6 treated. The results of histological and IHC analysis indicated that chondrogenic differentiation in TGFβ3 and the combination of TGFβ3 and BMP6 showed more cartilage deposition. In conclusion, TGFβ3 and BMP6 differentiated hUC-MSCs into chondrogenic clusters of the combination treatment of the two growth factors showed more efficient chondrogenic ability.
4,000원
8.
2012.12
구독 인증기관 무료, 개인회원 유료
Lysyl oxidase (LOX) is an extracellular amine oxidase catalyzing formation of lysine-derived cross-linkages in collagen and elastin in extracellular matrices. Four human paralogs of LOX (LOXL1, LOXL2, LOXL3, and LOXL4) have been identified, each encoding the functional domains required for the amine oxidase activity of LOX. Upregulated expression of LOX and LOXL2 was reported to show significant correlation with absence of lymphovascular invasion in tumor tissues from patients with colorectal adenocarcinoma, suggesting that oxygen tension around tumors may be an important factor in expressional regulation of these genes in colorectal carcinomas. To evaluate the effects of hypoxia on expression of LOX and LOXL2 in colorectal carcinomas, we performed promoter assays and RT-PCR analysis under hypoxia in colorectal carcinoma cell lines, HT-29 and HCT-116. Expression of LOX was upregulated under hypoxia in both HT-29 and HCT-116 cells, whereas LOXL2 expression was not affected by hypoxia in those cells. In the highly metastatic HCT-116 cells, LOX showed a higher level of upregulation than in HT-29 cells, suggesting a possible association of LOX upregulation with increased invasiveness and metastatic potential in colorectal carcinomas.
4,000원
9.
2012.12
구독 인증기관 무료, 개인회원 유료
The aim of this study was to investigate the morphological development pattern of conical papilla (CP) during prenatal and postnatal periods in native Korean goats by scanning electron microscopy. Tongues were removed from fetuses on days 90, 120, and neonates and from juveniles on days 30, 50, 60, 75, 90, 120, 150, and 180 after birth. The lengths of CP were 194~240 μm in neonates, 335~485 μm in the weaning period of 60-day-old goats, and 526~662 μm in the maturing period of 180-day-old goats. The primordia of CP in 90-day-old fetuses, shaped like mountain berry, were sprouted. The CP of 120-day-old fetuses was a cylinder shape with an apex whose center was slightly concave and the margin was sticking up like a low fence. The CP of neonates was empty inside and the secondary papillae were irregularly arranged fence-like structures on it. In 60-day-old goats, CP was an obliquely sectioned cylinder shape compacted in inner surface; the shapes of CP varied in 120-day-old goats, and had a smooth surface. A low elevation of CP was observed in 150- and 180-day-old goats. The microridges, microplicae, and micropits were well developed on the epithelial surface of lingual papillae in 60- to 120-day-old goats. These findings indicate that CP of goats has a variety of sizes and shapes during development.
4,000원
10.
2012.12
구독 인증기관 무료, 개인회원 유료
Gon-Sup Kim, Gyeong-Eun Hong, Hyeon-Soo Park, Jin-A Kim, Arulkumar Nagappan, Jue Zhang, Sang-rim Kang, Chung-kil Won, Jae-Hyeon Cho, Eun-Hee Kim
Fraxinus rhynchophylla (Oleaceae), a deciduous tree, is known to have properties that include anti-inflammatory, convergence, febricide, antiblenophthalmia, antidiarrhea, antileukorrhea, and so forth. In addition, it has been used for traditional herbal medicine in East Asian countries, including Korea. In this study, we investigated the antioxidant and anti-inflammatory effects of Fraxinus rhynchophylla ethanol extract (FRE) in lipopolysaccharide (LPS)-induced murine macrophage Raw 264.7 cells with FRE pretreatment. We performed DPPH-assay, Western blot, and Reverse Transcription-Polymerase Chain Reaction (RT-PCR). FRE showed 85% free radical scavenging activity at concentrations of 80 µg/ml. Results of this study also showed that FRE down-regulates Cox-2 and iNOS expression in mRNA and protein level. In conclusion, crude ethanol extract of Fraxinus rhynchophylla exhibited antioxidant and anti-inflammatory activities, and it may potentially provide a valuable source of natural herbal agent to inhibit inflammation.
4,000원
11.
2012.12
구독 인증기관 무료, 개인회원 유료
To clarify the role of stem cells in hepatocarcinogenesis, octamer-binding transcription factor 4 (Oct4) expression was investigated in mouse liver and embryonic cell lineages. In vivo, at 14 days of age, ten ICR mice were divided into two groups and treated with saline or diethylnitrosamine (DEN), and were sacrificed at 6 h after treatment. Livers were fixed in 10% neutral phosphate buffered formalin, embedded in paraffin, sectioned to a thickness of 5 μm, and immunohistochemical analysis of Oct4 was performed. In vitro, mouse embryonic stem cells, hepatic progenitor cells and hepatocytes, representing 0, 22, and 40 days of differentiation, respectively, were treated with DEN at four doses (0, 1, 5 and 15 mM; G1, G2, G3 and G4, respectively) for 24 h and RNA was isolated; Oct4 and Gadd45a mRNA were investigated. In vivo, Oct4 expression was not detected in saline-treated livers. However, its expression was observed in hepatocytes of mice treated with DEN, showing cytoplasmic staining. In vitro, Oct4 expression differed significantly for G4 on day 0 (P<0.05) and for G2 on day 22 (P<0.01) and G3 and G4 on day 40 (P<0.05 and P<0.01, respectively) compared with G1 at each time point. Gadd45a expression differed significantly in G4 (P<0.01) on day 0 and G4 on day 40 (P<0.01), compared with that of G1 at each time point. Taken together, Oct4 expression was increased by treatment with DEN in hepatocytes, however, not in embroyonic stem cells and hepatic progenitor cells. This finding suggests that Oct4 expression may be modulated in hepatocarcinogenesis induced by DEN.
4,000원
Case Report
12.
2012.12
구독 인증기관 무료, 개인회원 유료
A three-year-old Russian Blue female cat was presented for long-standing infertility and recent development of male behavior. From one to three years of age, the cat had been bred, however, pregnancy never resulted. Abnormal enlargement of the uterus and ovaries was observed by abdominal sonography and radiography. An ovariohystectomy was performed. In the uterus, adenocaricnoma lesions were found and characterized microscopically by replacement of the endometrium with a population of large polyhedral cells with a tubulopapillary or nested arrangement and a supporting fibrous stroma with minimal vascularization. In addition, the pedunculated mass was diagnosed as leiomyoma, which was continuous with the myometrium, composed of closely packed sheets of spindle cells embedded in vascularized stroma, and covered with a discontinuous endometrial epithelium. The ovarian neoplasm was diagnosed as an ovarian interstitial cell tumor. Based on the histopathological characteristics, this case was diagnosed as a simultaneous occurrence of an ovarian interstitial-cell tumor and uterine adenocarcinoma and leiomyoma in a female cat. To the best of our knowledge, this report represents a rare case of multiple tumors in the genital organs of a cat.
3,000원
Short Communication
13.
2012.12
구독 인증기관 무료, 개인회원 유료
Hung Sun Koh, Beong Kug Yang, Bae Keun Lee, Kyung Hee Jang, Seong Teak In, Jong Hyek Lee, Kwang Seon Kim, Gu Hee Kweon
To identify subspecies of the sika deer, Cervus nippon, and to select C. n. hortulorum individuals for restoration of this subspecies in Korea, we obtained cytochrome b partial sequences (375 bp) of sika deer (C. nippon) from deer farms in northeastern China, North Korea, mainland Korea, and Jeju Island. Based on noninvasive samples, these cytochrome b sequences were compared with the corresponding haplotypes of sika deer, which were obtained from GenBank. We identified five individuals of sika deer from deer farms in northeastern China and North Korea as C. n. hortulorum, and found that interbreeding between individuals belonging to different subspecies is common on deer farms in far-eastern Asia. We concluded that the five C. n. hortulorum individuals on deer farms from northeastern China and North Korea, detected in this study, cannot be considered as pure descendants of C. n. hortulorum in the wild.
3,000원
14.
2012.12
구독 인증기관 무료, 개인회원 유료
Lactic acid bacteria (LAB) are known to promote activation of macrophages, selectively eliminate harmful intestinal bacteria that enter the body, and increase secretion of cytokines, chemokines, and inflammatory mediators that boost the immune system. In the current study, the immune-enhancing effects of five Leuconostoc stains (Leu. kimchii WK18, Leu. citreum DH33, Leu. mesenteroides DH34, SE34, and GY17) isolated from traditional Korean fermented food, Kimchi, were evaluated. Lactobacillus rhamnosus GG (LGG), which is known for its extraordinary immunopotentiating activity, was used as a reference strain. According to the results, several heat-killed Leuconostoc strains induced a higher level of NO, IL-1β, or TNF-α production than the LGG strain. Collectively, results of the current study suggest that Leuconostoc strains could enhance immune response through activation of macrophages, and could be significant references when verifying the possibility for use of Leuconostoc strains as probiotics, starter, dietary supplement, or immune-enhancing medicine.
3,000원
