표고버섯 과립의 품질 특성에 결합제가 미치는 영향을 조사하기 위하여, 버섯분말에 결합제로 corn starch, lactose, gelatin, gum arabic 및 dextrin(DE=23)을 각각 첨가하고 버섯열수추출액으로 반죽하여 압출과립을 제조한 다음 과립의 이화학적 특성을 측정하였다. 과립의 용해도는 gum arabic >gelatin>lactose, dextrin>corn starch 첨가구 순이었다. L값은 corn starch l

The extracts of Pinus densiflora sawdust by n hexane, ethyl acetate and methanol solvent were investigated to identify their mycelial growth inhibition against Lentinus edodes. The yields of n hexane soluble fraction, ethyl acetate-soluble fraction, and methanol soluble fraction from P. densiflora sawdust were obtained 1.36%, 2.21% and 4.03% using organic solvent, respectively. The mycelial growth inhibition of L. edodes was the greatest for n hexane extract, ranging from 36.5% to 47.6% at concentrations of 125 ppm to 1,000 ppm, with the values for all concentrations significantly different from one another. After direct extraction of P. densiflora sawdust using n hexane, ethyl acetate and methanol, each extract was separated into three fractions by silica gel column chromatography and then the fractions were isolated on the values of Rf by thin layer chromatography. The mycelial growth inhibition against L. edodes was recognized in the fractions II (33.5%) and III (37.6%) of n hexane extract, the fraction II (21.4%) of ethyl acetate extract and the fraction II (26.4%) of methanol extract. The fractions III of n-hexane extract showed the highest growth inhibition among the nine fractions of the organic solvent extract.

표고버섯을 새로운 형태의 식품으로 개발하기 위한 기초 자료와 천연보존료로의 이용 가능성을 검토하고자 물과 에탄올로 항균성 물질을 추출하여 몇 종의 병원균, 식중독균, 식품과 관련이 있는 세균 및 효모등 10균주에 대하여 항균성 여부와 추출물의 최소저해 농도를 밝히고, 표고버섯추출물을 용매 계통 분획하여 항균활성을 살펴보았다. 항균성 검색에 사용된 물과 에탄을 추출물 모두에서 대부분의 세균은 항균활성이 나타났으나, 효모와 젖산균에서는 나타나지 않았

The availability of enokitake cultural waste for Lentinus edodes cultivation was investigated, although hardwood sawdust has traditionally been used as a substrate for this fungus. Firstly, physiochemical characteristics of cultural waste were analysed. Secondly, mycelial growth characteristics and fruiting yields of L. edodes on waste treated in some methods were determined. Physiochemical characteristics of enokitake cultural waste showed that the millwaste complex was a little degraded by enokitake fungus and suggested the probability that most component lost by enokitake could be rice bran. Mycelia of L. edodes grew and fruited well on waste supplemented by fresh rice bran and Quercus sawdust although didn't on waste only. Mycelial growths of these fungi on waste were accelerated when supplemented by rice bran to the percent of 40(w/w) but decreased or suppressed at above ratios(30, 40%, w/w). Supplementations of oak sawdust at above 40%(w/w) of the waste and rice bran at 20%(w/w) of the sawdust allowed such a good mycelial growth as to be selected as a pertinent mixing ratio for fruiting medium. A fruiting yield on enokitake cultural waste supplemented by oak sawdust (at 40% of the waste, w/w) and rice bran (at 20% of the sawdust, w/w) was not inferior to that on oak sawdust supplemented by rice bran only (at 20% of the sawdust, w/w). These results indicated strongly the potentiality of enokitake cultural waste as raw materials for shiitake cultivating substrates.

Changes of free sugars and sugar alcohols of fresh shiitake were measured during storage. Shiitake were stored at 1 and -4, with non-packaging, wrapping, 0.03mm polyethylene(PE)film packaging(unpunched and punched, unprecooled and precooled before storage), 0.05mm PE film (vacuum packaging). Chanties of glycerol, arabitol and arabinose contents were not marked during storage. The content chanties of -rhamnose, fructose, glucose, mannitol, sucrose and maltose were the smallest until 40days storage and were increased significantly after 40 days and then decreased significantly after 50days. Content of -rhamnose during storage when was treated unprecooling, 0.03mm PE film punched and unpunched at -4 was the highest 25.08% and 34.86%, respectively in 50~60days. Content of mannitol was not changed at -4.

표고의 다공(多孔) 접종재배(接種栽培)에 의한 접종량(接種量)의 증가(원목(原木) m3 당 3.0kg - 6.0kg)가 균사(菌絲)의 초기 발육 상태인 활착(活着), 만연(蔓延)과 숙도(熟度) 그리고 우실체(宇實體)의 생산성(生産性)인 5년간 발생량과 개체당 중량에 미치는 영향을 조사한 결과 다음과 같은 결론을 얻었다. 1 다공(多孔) 접종재배(接種栽培)에 의한 종균(種菌) 접종량(接種量)의 증가는 종균(種菌) 활착율(活着率)에 영향을 주지 않았으며 균사(菌絲) 만연율(蔓延率)과 균사(菌絲) 숙도율(熟度率)을 증가시켰으며 결과적으로 자(字) 실체(實體)의 발생량도 증가되어 대조구인 골목m3당 접종량(接種量) 3.0kg구의 발생량은 16.34kg/m3이었으나 접종량(接種量) 6.0kg구에서는 7.7% 증가되어 17.60kg/m3 이었다. 2. 다공(多孔) 접종재배(接種哉培)에 의한 종균(種菌) 접종량(接種量) 증가는 자실체(字實體)의 조기 발생을 나타내었다. 자실체(字實體)의 2년차와 3년차의 건조 중량 발생량은 대조구인 골목 m3당 접종량(接種量) 3.0kg구는 5년간 발생량의 62.7%인 10.25kg/m3인 반면 비교구인 접종량(接種量) 5.0kg구-6.0kg구는 74.0%-82.7%인 12.82kg/m3-14.56kg/m3으로 집중적으로 발생하였고 4년차와 5년차에서의 발생량은 급격히 감소하였다. 3. 표고 자실체(字實體)의 월별 발생량은 다공(多孔) 접종재배(接種栽培)에 영향을 받지 않았다. 자실체(字實體)는 5월-6월과 8월-9월 동안에 가장 많이 발생하였으며 발생량은 당해 년도 발생량의 11%-33%과 16%-28%를 각각 차지 하였다. 4. 다공(多孔) 접종재배(接種栽培)에 따른 접종량(接種量)의 차이에 의한 자실체(字實體) 개체당 평균 중량(건조 중량)은 일정한 경향이 없었으나 골목 m3당 접종량(接種量) 4.0kg구가 1.63g로 가장 큰 것으로 나타났다.