Salicornia europaea L. (SE) as a halophyte has been widely used as a seasoned vegetable with various biological activities, including anti-inflammatory and anti-diabetic activities. In particular, the desalted SE (DSE) has been considered an attractive alternative compared to SE for its anti-obesity effect. 1-Deoxynojirimycin (1-DNJ) is one of the known α-glucosidase inhibitors and has been reported to have similar biological activities with SE. This study reports the anti-obesity and anti-inflammatory activities of DSE fermented by Bacillus velezensis K26 (BVDSE) previously isolated from Korean fermented soybean paste and elucidated to produce 1-DNJ in 3T3-L1 adipocytes and RAW264.7 cells. BVDSE (5%) did not show cytotoxicity in 3T3-L1 cells and inhibited lipid accumulation, which was decreased to 23% in the differentiated cells. BVDSE did not inhibit the release of the pro-inflammatory cytokine, TNF-α, while it remarkedly inhibited IL-6 on lipopolysaccharide-induced RAW264.7 cells. Taken together, these results indicate that the fermentation of DSE with B. velezensis K26 as a 1-DNJ producer has more significant anti-obesity and anti-inflammatory effects than even non-fermented DSE, assuming the synergistic effects by 1-DNJ production from B. velezensis K26, by which it could be highlighted that BVDSE may be considered for use as a functional food supplement.
Total 24 endophytic fungal strains were purely isolated from Salicornia europaea L. native to representative saltern of the Korea, Gomso. Strains were partially identified using amplified internal transcribed spacer(ITS) regions and the phylogenetic tree were constructed. Furthermore, deduction of two diversity indices was done. Endophytic fungal isolates belonging to 9 genera, concretely in Aspergillus, Aureobasidium, Cladosporium, Fusarium, Gibberella, Macrophoma, Penicillium, Phoma, Stemphylium, and unidentified(Pleosporales), respectively. Especially, Aspergillus(29%), Penicillium(21%) and Fusarium(13%) were dominantly distributed genera from the roots of S. europaea. Among them, genera Aspergillus, Aureobasidium or Gibberella have been reported with their activities of plant growth promoting, induces systemic resistance in previous researches. This study is dealing with the securing the diversity of endophytic fungi that showing symbiotic relationship with their host halophyte S. europaea which take the counter stage as a new crop resource.
신선 퉁퉁마디의 저장성 향상을 위해 MAP에 적절한 포장재와 저장온도를 구명하고자 본 실험을 수행하였다. 저장중 생체중 감소는 10℃ 이하의 저온과 무공필름에서는 2% 이하로 유지되었으나 25℃의 유공필름처리는 4% 이상의 감소를 보이면서 품질저하가 발생하였다. 저장중 포장재내 대기조성은 필름 투과율에 비례하였는데 이산화탄소는 1~2%, 산소는 15% 이상으로 유지되었다. 25℃를 제외한 모든 처리에서 저장 중 포장재내 에틸렌 농도는 7일 이후 급격히 증가하였는데, 2℃에서 가장 높아 저온 장해가 발생한 것으로 추측되었다. 저장 최종일에 이취와 부패율은 5℃에서 가장 낮았으며, 포장재별로는 다른 양상을 보였는데 저장온도중 가장 낮았던 5℃에서는 50μm 두께의 ceramic 필름이 가장 낮은 이취와 부패율을 보였다. 외관상 품질로 본 저장수명 역시 5℃에서 50μm ceramic 필름으로 포장하였을 때 28일 이상으로 가장 길었다.