본 연구는 시설토마토 재배지에서 push-pull 전략을 이용해 담배가루이와 천적곤충의 행동을 제어함으로써 담배가루이(Bemisia tabaci)를 친환경적으로 방제하기 위한 기술을 개발하고자 수행하였다. 담배가루이는 노란색에 유인율이 가장 높았으며, 520 nm의 광원에는 유인반응을 복합광원인 450 + 660 nm의 광원에는 회피반응을 보였다. 천적곤충인 담배장님노린재(Cyrtopeltis tenuis)와 미끌애꽃노린재(Orius laevigatus)는 모두 520 nm의 광원에 가장 높은 유인반응을 보였다. 휘발성 물질로는 ocimene과 carvacrol에 대해 기피반응을, methyl isonicotinate에는 유인반응을 보였다. 토마토 온실에 메밀을 투입하였을 경우 천적곤충인 담배장님노린재의 밀도는 무처리 대비 15일간 약 16배로 높게 유지되었다. Push-pull 세부전략들을 종합 처리한 결과, 처리 50일 경과 후 담배가루이의 트랩당 밀도는 무처리 대비 3배 이상 낮게 나타났으며, 이에 따른 시기 별 방제효과는 시간이 경과함에 따라 증가하는 경향을 보인 가운데 최고 68.7%였다.

This study was conducted to develop a technology for environmentally friendly control of Bemisia tabaci by a push-pull strategy in tomato greenhouse. B. tabaci was most attracted to the light source of 520nm, whereas it showed an avoidance response to the complex light treatment of 450+660nm. Two natural enemies, Nesidiocoris tenis and Orius laevigatus were attracted to 520nm. B. tabaci was repellent to volatile organic compounds, ocimene and carvacrol, while methyl isonicotinate showed attractant response. When buckwheat was added into the greenhouse, the density of N. tenis was maintained at about 16 times higher for 15 days. As a result of the combined treatment of push-pull strategy, the density of B. tabaci was 3 times lower and the control effect increased with time and reached up to 68.7%.

경기지역에서 담배가루이의 발생 및 약제반응을 조사하였다. 담배가루이는 13개 시군에서 발생되었으며, 유전자(16S rRNA, MtCOI) 분석결과, 고양시 일부지역은 biotype B와 Q가 혼재되었으나, 그 밖의 지역에서 2005부터 국내 발생이 보고되었던 biotype Q로 나타났다. 채집곤충 및 기주식물의 total DNA에 대해 PCR을 통한 염기서열 분석과 southern hybridization 분석 결과 모든 지역에서 Tomato Yellow Leaf Curl Virus(TYLCV)는 검출되지 않았다. 5개 지역에서 채집한 6 개체군의 담배가루이를 실내사육하면서 유묘검정법으로 성충에 대한 살충제 감수성을 검정한 결과, 지역별로 다소 차이는 있었지만 네오니코티노이드계 약제의 경우 biotype B에게 높은 살충활성을 나타낸 반면 biotype Q에게는 상대적으로 낮은 살충활성을 나타내었다.

The green peach aphid(Myzus persicae) and sweet-potato whitefly(Bemisia tabaci) biotype Q are serious pests in hot pepper and cucumber. To optimize the formulation type of Clothianidin 1.8% granule, coated and extruded granule formulation types were tested against green peach aphid in hot pepper and sweetpotato whitefly in cucumber. Clothianidin 1.8% granules were incorporated in soil before transplant of hot pepper and cucumber. At recommend dose(540g ai/ha), the efficacy of Clothianidin 1.8% coated and extruded granules were 83.3% and 94.4% and continued until 50 days and 70 days against green peach aphid, respectively. Against sweet-potato whitefly, the efficacy of Clothianidin 1.8% coated and extruded granules were 65.7% and 96.3% at 32 days in cucumber, respectively. Clothianidin 1.8% extruded granule was safe on crops (hot pepper, cucumber, melon, cabbage etc.) at 540g ai/ha (recommend dose) and 1080g ai/ha. The results suggested that Clothianidin 1.8% extruded granule is promising as a best insecticide against green peach aphid and sweet-potato whitefly, because of the high efficacy and low phytotoxicity.

Sweet potato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is a serious pest of many economically important crops. The insect has developed resistance to chemical insecticides. Therefore, the development of microbial agent such as entomopathogenic fungi, Lecanicillium lecanii is necessary. Spores of L. lecanii Btab01 was collected after cultivation on solid PDA and liquid RB (rice bran amended with 2% molasses) media. The bioassay was carried out with B. taabci nymphs for 7 days at 25℃ and 60% relative humidity. Further, mortality was corrected with appropriate controls. The results revealed that spores obtained from RB medium caused high mortality (98.31%) compared to PDA medium (93.94%). Spore concentrations 105, 106, 107, and 108 colony forming units (c. f. u) ml-1 caused 5.81, 33.80, 77.27, and 98.54% mortality, respectively. The mortality (100%) was observed for 4 days when L. lecanii spores was mixed with 0.1 - 0.3% soybean oil. Hence, it is concluded from this study, L. lecanii Btab01 cultivated on RB medium can be recommended to control the nymphs of B. tabaci. Spore suspension can be expected to high efficacy when soybean oil was blended.

Dongbu HiTek had a focal group depth interview with pesticide sales dealers and farmers in 2007. With results, dealers assessed a white fly as a most difficult pest in up-land crop cultivation, and answered the increase and change of pests and pathogen from climate change as a most concerned event. Farmers pointed that important jobs in vegetable cultivation were the control of pest and pathogen (34%) and the prevention of pest and pathogen (24%). And difficult works was the control of pest and pathogen (24%), and the climate change (18%). When choosing the insecticide, the long lasting activity (44%) and the completeness of control (34%) were the important factors. Among customers’ claims related with white fly, claims on insufficient efficacy was dominant and occupied 86% in spite of efforts of producers, and it reflected that commercial agrochemicals did not meet the expectation level of the farmers. Common agrochemicals for the control of white fly are Neo-Nicotinoids in Korea today and CGAR of it is about 13% average increased per year from 2003. As Bio Type Q showed higher resistant level to the Neo Nicotinoids than other Bio type (Nauen et al. 2002), Bio Type Q would spread easily and counter-plan is needed.. Market size of agrochemical for white fly is assumed to be about 9.6 million US dollars. The market size is smaller than that of aphids or lepidopterous pests, but the market would increase because of suffering and failure in control due to resistance and resurgence problems. As the increase of vegetable consuming, host crop for the white fly is also increasing. As green house cultivation area of host vegetable increases, natural enemies to foreign invading pest like sweet potato white fly are limited. Also it is important that that management of pest and pathogen in green house environment. It is essential to build pest management model and to develop and commercialize agrochemicals according to the critical customer (farmer) requirement.

For effective control of insect pest which can outbreak in a field during crop cultivation, various control agents such as eco-friendly agricultural materials (EFAM) including microbial control agents and chemical pesticides have been applied at fields and these control agents may be treated simultaneous or sequential in the same field to suppress diverse pests and diseases. The agents may influence each other and control efficacy may also differ from interactions. Therefore we need to test compatibility of microbial control agents with other agricultural agents. In this study, we investigated influence of pre-treated EFAMs, which are registered for whitefly control in greenhouse, on germination, mycelial growth and control efficacy of Isaria javanica isolate against sweet potato whitefly. The results showed that a mixture of paraffin oil+cinnamon oil among 4 EFAMs highly reduced germination (8.9±1.3%~24.5±0.9%) and mycelial growth (0.81±0.01cm~0cm) of I. javanica. To investigate the effects of the treatment interval between EFAMs and I. javanica on sweet potato whitefly control, four different EFAMs were pre-treated 0, 1, 4, and 7 days before applying I. javanica. Pre-treatment of four EFAMs inhibited insecticidal activity of I. javanica against sweet potato whitefly. Therefore when EFAMs and a mycopesticide using I. javanica spray simultaneous or sequential, application of EFAMs need more than 7 days interval after treatment of mycopesticide at field.