1. 세계 26개국에서 도입된 녹두 유전자원 705점에 대하여16개의 형태적 형질 특성을 이용하여 각각 두 가지의 군집방법, 핵심집단 자원수 결정방법 및 표본추출 방법을 조합하여 총8개의 핵심집단(SUNPR, SUNPPr, UNPR, UNPPr, SUNLR, SUNLPr, UNLR, UNLPr)을 작성하여 원수집단과 비교한바 8개핵심집단 모두 원수집단과는 분산과 평균치에서 차이가 없었다. 2. Nei의 다양성지수는 8개 핵심집단 모두 원수집단 보다높았고, 특히 UNLPr이 가장 높았으며, UNPR은 다른 핵심집단에 비하여 상대적으로 낮은 지수를 보였다. 3. 원수집단과 핵심집단간 자원분포의 동질성을 파악하기 위하여 카이자승 검증을 한 결과 12개 양적형질과 1개의 질적형질(종피색)은 모든 핵심집단이 동질분포를 보였으나 3개의질적 형질 (배축색, 종피광택, 생육습성)은 핵심집단에 따라서동질분포를 하는 것과 그렇지 않은 것이 있었으며, 8개 핵심집단 중 UNPPr은 동질성이 가장 높았다. 평균차율(MD%)과일치율(CR%)은 8개 핵심집단 모두 유의한 수준을 보였다. 4. 8개의 모든 핵심집단이 원수집단과 평균치에서 차이가 없고, 높은 다양성을 과 동질한 분포를 보이며, 평균차율(MD%)과 일치율(CR%)이 유의한 수준을 보여 원수집단의 다양성을잘 나타내는 것으로 해석되었다. 5. 핵심수집단 크기(10%와 15%)에 따른 다양성은 유의한차이가 없었으며, 8개의 핵심집단 중 평균치, 동질분포, 일치율 및 도복내성을 고려할 때 UNPPr이 가장 좋은 핵심집단으로 사료되었다.

Mungbean is an important crop in Nepal. Heteropteran bugs, Nezara virudula, Dolycoris baccurum, and Melanacanthus scutellaris are significantly influencing qualitative and quantitative loss in soybean and mungbean. Though pesticide application is common practice for management of these bugs, pesticide avoidance by the bugs stalls proper management. As an alternative, trap cropping with variety mostly preferred by the bugs as trap crops along with the main crop is considered. Selection of most preferred mungbean variety was performed with six (VC 6173 A; VC 6153 B-20G; VC 3960 A-88; Kalyan; Pratikchha; Saptari local) relative to Saptari local variety. Significant difference was recorded in number of bugs occurrence among the six varieties with maximum number of bugs in VC 6173 (3 bugs/plant) which showed lowest yield. For application, three different trap crop designs were employed in the field. There was significant difference between the main crop i.e. variety Pratiksha and the variety VC6173A used as trap crop in two designs. In conclusion VC6173A is a probable trap crop and the two designs can be employed in the field.

In this study, we examined the variation of 30 phenolic compounds in the mungbean (Vigna radiata (L.) Wilczek) germplasms. Recent studies showed that the potential health benefits from the antioxidnant activity of the phenolic compounds. The average concentration of phenolic compounds among the 70 mungbean germplasms was 2424.9 μg g-1. Among the mungbean germplasms, 25 kinds of mungbean germplasms were higher than others. Specially, 69 (IT 182280) (3836.63 ㎍ g-1) and 63 (IT 180525) (3491.51 ㎍ g-1) revealed highest levels of the total phenolic compounds. On the other hand, 27 (IT 154078) (1624 ㎍ g-1) was revealed lowest concentration of the total phenolic compounds. Among the individual phenolic compounds, rutin (avg. 1776.09 ㎍ g-1) showed highest concentrations, pyrogallol (144.31 ㎍ g-1), gentisic acid (119.19 ㎍ g-1) and chlorogenic acid (78.24 ㎍ g-1) were relatively higher than other phenolic compounds. The germplasm of 69 (IT 182280) had the highest rutin (3156.87 ㎍ g-1) concentraion. While, the biochanin A (avg. 2.02 ㎍ g-1) and formonetin (avg. 2.61 ㎍ g-1) had the lowest levels among the mungbean germplasms. This study indicateded that determination of the functional substances in mungbean germplasms and it is more valuable for the future crop cultivation and food industries.

2,500여 녹두 유전자원 중에서 작물학적 가치를 가진 789계통을 선발하고, 2005년과 2006년에 각각 401, 388계통을 대상으로 배축색, 엽 크기 등 27가지 형질특성을 조사하고 종실의 vitexin과 isovitexin 함량을 분석하였다. 2005년 시험에 이용된 401계통의 평균 vitexin과 isovitexin 함량은 각각 8.7(1.1~13.4), 9.5(0.9~15.9) mg/g이고, vitexin과 isovitexin 함량의 상관계수(R2)는 0.958었으며, VC3890B 등 vitexin과 isovitexin 고 함유 자원 9계통을 선발하였다. 2006년 시험에 이용된 388계통의 평균 vitexin과 isovitexin 함량은 각각 10.2(2.0~15.9), 10.6 (0.6~17.6) mg/g이고, vitexin과 isovitexin 함량의 상관계수(R2)는 0.958었으며, VC 4096-2B-4-B-2-B 등 vitexin과 isovitexin 고 함유 자원 6계통을 선발하였다. 녹두 주요 형질 중에서 엽이 클수록, 등숙기간이 길수록 vitexin과 isovitexin 함량이 많은 경향이었다.

We isolated low temperature inducible genes using suppression subtractive hybridization (SSH) method and were able to obtain to clone MLT7 gene encoding peroxiredoxin and aminotransferase. The full-length cDNA of MLT7 is 1,049 bp with an open reading frame (ORF) consisting of 261 amino acid (aa). Genomic southern blot confirmed that mungbean genome has two copies of MLT7 gene. Northern blot analysis was also carried out for the gene expression during ABA, NaCl, drought, wounding and H2O2 stresses. The expression of MLT7 gene significantly decreased by ABA, NaCl and drought stress, but wounding and H2O2 stress significantly induced MLT7 gene expression. Especially, H2O2 strongly induced the MLT7 gene expression. The expression of MLT7 gene during low temperature stress started to increase in 3 h after treatment, and than slightly decreased and again increased at 24 h. Using GFP fusion vector, GFP-MLT7 was targeted both to mitochondria and chloroplast. However, it was mostly targeted to mitochondria and partially targeted to chloroplast. For the functional analysis of MLT7, MLT7 recombinant protein was heterologously expressed in E. coli. The MLT7 recombinant cells showed enhanced antioxidant activity compared to that of vector control cells.

다량의 페놀류 2차 대사산물과 다당류가 포함된 녹두 종자의 RNA를 Tris-buffer와 guanidium thiocyanate 용액을 이용하여 손쉽고 간편하게 고순도의 RNA를 분리하는 방법을 확립하였으며, 우리의 분리 방법을 이용하여 추출한 RNA를 사용하여 RT-PCR과 유전자 클로닝 등의 분석시험에 이용할 수 있었다.

Mung bean (Vigna radiata L.) is an increasingly important human food source, as well as a new functional agent, mainly due to its potent antioxidant activity. This study was conducted to determine antioxidant activity of fractions from mung bean seeds by measuring DPPH radical scavenging activity and to quantify the flavonoids by means of HPLC analysis. Vitexin and isovitexin were present in both ethanol and water extracts in highest amount. Flavonoids, vitexin and isovitexin were quantified from 195 germplasms of mung beans and their concentrations varied by 4.7 fold. Especially, the breeding line KM99004-4B-2 (Suwon28/KM94004), which has grown in Jeollanamdo Agricultural Research and Extension Services, showed the highest amount (15.88 mg/g) of total flavonoids. The vitexin portion was averaged 70.73±1.38%. High positive correlation (r=0.96***) between vitexin and isovitexin contents showed. However, the flavonoid content showed very low correlation with the 24 growth and ecological characteristics. Seed coats of mung beans had the highest flavonoid amount, showing 50~70 times more than cotyledons. Flavonoid contents in the seed, the cotyledon, and the seed coat were decreased as the seed imbibition time increased.

Combining ability study was carried out on the components of synchronization in maturity and determinate growth habit in mungbean, using 6~times 6 diallel cross. Both additive and non-additive gene effects were found conditioning the inheritance of days to first flower, days between first pod and 90% pod maturity (DDd1), plant height from first pod stage to 90% pod maturity (DDhl, DDh2, and DDh3). Only non-additive gene action was important in degree of determination from first pod stage to 90% pod maturity (DDd2). While only additive action was important in plant height at first flower. The predominant additive gene action was observed in all traits but non-additive was significant in only DDd2 . For synchronization in maturity, determinate growth habit, and their components, the best combiners were NM92, VCl560D, and NM89, whereas the best indeterminate combinations were NM92 ~times NM89, NM92 ~times VCl560D, and NM92 ~times ML-5.

Combining ability in mungbean was studied in 15 quantitative traits through a 6 ~times 6 diallel cross. Both additive and non-additive gene effects were found conditioning the inheritane of nodes of the first peduncle, clusters per plant, clusters on main stem and branches, pods per plant, 1000 seed weight, grain yield per plant, biomass, and harvest index. The additive gene action was found significant for nodes on main stem, average internodal length, branches per plant, pods per cluster, pod length, and seeds per pod. The predominace of additive genetic variance was observed in all traits. For grain yield and yield components, the best combiner were VC3902A, VC1560D and ML-5, while the best combinations were the crosses VC3902A ~times ML-5, VC1560D ~times ML-5, and NM 92 ~times VC1560D

본 시험은 녹두재배기술의 기초자료를 얻기 위하여 녹두의 지상부 형질, 수량구성요소, 수량 및 종실성분 변화를 구명코자 숙전 및 개간전에 질소, 인산, 가리의 시비량을 달리하여 시험하였던 바 그 결과를 요약하면 다음과 같다. 1. 경태는 토양류형에 관계없이 식물체 중앙부인 제5절간 > 기부절간 > 상위부위인 제9절간 순으로 굵었으며 특히 제5절간은 3요소시용과 상관이 있었다. 2. 식물체 상위 4개 절위의 엽병각은 숙전에서보다 개간전에서 증대되었다. 3. LAI는 어는 생육시기에서나 숙전에서 높았다. 특히 3요소 시용 및 3요소＋퇴비시용구에서 높았으며 개간전에서는 인산의 효과가 현저하였다. 4. 개화후 20일의 LAI가 클수록 건물중 및 종실수량은 많아졌으며 NAR은 낮아졌다. 5. 숙전에서는 가리시용, 개간전에서는 인산시용에 의한 주당협수의 증가로 종실수량이 증가하였다. 6. 종실성분중 유지함량과 단백질함량과의 부의 상관이 있었다. 7. 종실수량에 크게 영향하는 것은 개체당 협수로서 협수와 종실수량과는 정의 상관이 인정되었다.