Sensory electrophysiological recording techniques such as EAG (electroantennogram), GCEAD (coupled gas chromatograph-electroantennogram detection) and SSR (single sensillum recording) have been useful in the chemical ecology studies of insects and plants. Numerous pheromones and other semiochemicals have been identified through GCEAD analysis, and the response profiles of antennae and individual olfactory receptor neurons have been characterized by using EAG and SSR techniques. In this talk, the practical aspects of these techniques are presented in detail. Standard setup and procedure of each electrophysiological recording technique, and important parameters and proper data analysis methods will be introduced as well as its applications. Common mistakes and limitations of these techniques will also be discussed.

Spotted wing drosophila (SWD), Drosophila suzukii, is a serious pest of berry fruits and grapes in East-Asia and USA. SWD female oviposits eggs into the skin of ripening fruits, and the larvae dig a burrow inside the fruits. Secondary infestation by other insects and pathogens to the oviposition spots cause substantial economic damages worldwide. SWD has recently been spreading to Europe and USA, and it is becoming a global threat in terms of biosecurity. A quite useful paper has been published in Journal of Chemical Ecology in the most recent issue (Vol. 44, #10) which is titled ‘Advances in the chemical ecology of the spotted wing drosophila (Drosophila suzukii) and its applications’ written by Cloona et al. This article introduces chemical constituents from many odor sources such as fermentation, yeast, fruit, and leaf sources, and commercial lures and traps developed using these compounds. Authors said that the current food-based lures and trap technologies are not expected to be specific to SWD and thus capture large numbers of non-target drosophilids. Attractive and aversive compounds are being evaluated for monitoring, mass trapping, and for the development of attract-and-kill and push-pull techniques to manage SWD populations. (Supported by PJ011756022018, RDA)

Effective phytosanitary fumigation can prevent the introduction of exotic insects into new areas where they become pests. Traditionally Probit 9 level control (99.9968%) was considered as a stand-alone quarantine treatment. However, unacceptable phytotoxic damage often associated with high-dose treatment to realize Probit 9 level control of a large number of pests has often restricted its practical application. Therefore, quarantine security is being achieved for some commodities using a “systems approach”, where quarantine pests are cumulatively reduced to acceptable levels using independent, pre- and postharvest measures that comprise a systems that effectively mitigate the pest. Preharvest reductions in population through the study and application of chemical ecology involving insect behavior modifying chemicals can be an integral part of systems approach. This results in low pest prevalence prior to end point treatment, where pests may be effectively controlled using a less-than-Probit 9 level, low-dose treatment. In this talk, I discuss the chemical ecology of invasive quarantine pests including Drosophila suzukii and its perspective in the effective end point fumigation treatment using ethyl formate.

최근 기후변화로 인하여 다양한 산림곤충들이 대면적으로 발생하여 산림수목에 큰 피해를 주고 있다. 그러나 산림해충의 정확한 발생모니터링이 이루어지고 있지 않으며 대부분의 방제 또한 유기합성농약에 의존하고 있다. 페로몬은 해충의 정확한 발생 모니터링뿐 아니라 대량포획 및 교미교란에 의한 친환경 방제방법으로 널리 활용 될 수 있다. 특히 산림은 다양한 생태계로 구성되어 있기 때문에 유기합성농약을 줄이거나 대체 할 수 있는 방제방법이 시급한 실정이다. 본 발표에서는 주요 산림해충인 천공성 해충류, 곰솔의 주요 해충인 솔껍질깍지벌레, 나비목 해충류에 대한 근래 연구동향에 대해 소개하고 미래의 산림해충 화학생태학의 연구 방향에 대한 제언을 하고자 한다.

Bacterial cells communicate with each other through their specific chemical languages, by which bacteria regulate a set of genes in a multicellular behavior, including antibiotics, biofilm, motility, and virulence. The twitter communication in Gram positive bacteria is mediated by diffusible signal molecules, N-acyl homoserine lactones (HSLs), that differ with respect to the length, saturation and substitutions of the side-chain. In Gram negative bacteria, diffusible peptides control the physiological phenotypes. In this presentation, some general examples of bacterial twitter communication, such as Vibrio fischeri, Burkholderia glumae, Agrobacterium tumefaciens, and Serratia marcescens will be presented. The contribution of bacterial communication to pathogenicity of Pantoea ananatis, our model bacterium causing bacterial rice sheath rot and onion center rot diseases, will mainly be discussed.

Conventional chemical ecology approach for identification of semiochemicals from natural sources is based on isolation of active ingredients after fraction and bioassay. Instead, we identify potential semiochemicals using reverse chemical ecology approach. In this way, we discovered an odorant receptor (CquiOR136) for DEET, which was not know how and why it work. Additionally, we identified a link between this synthetic repellent and methyl jasmonate, thus suggesting that DEET might work by mimicking defensive methyl jasmoante from plant. Moreover, we serendipitously found that a receptor, CquiOR36, responded to an old sample of nonanal in ethanol. Careful investigation led us to identify the active ligand as acetaldehyde (derived from ethanol) from “contaminants” in the old nonanal sample. We explored that acetaldehyde in a wide range of dose both attracts gravid mosquito and stimulate them to lay eggs in oviposition trays.

Herbivorous insects use plant metabolites to inform their host plant selection for oviposition. These host-selection behaviors are often consistent with the preference-performance hypothesis; females oviposit on hosts that maximize the performance of their offspring. However, the metabolites used for these oviposition choices and those responsible for differences in offspring performance remain unknown for ecologically-relevant interactions. In this time, I will talk about the host-selection behaviors of two sympatric weevils, the Datura (Trichobaris compacta) and tobacco (T. mucorea) weevils in field- and glasshouse-experiments with transgenic host plants specifically altered indifferent components of their secondary metabolism. In addition, I will show that adult females are able to choose the best host plant for their offspring and use chemicals different from those that influence larval performance to inform their oviposition decisions.

Chemical ecology has been useful in various aspects of biosecurity that requires dynamic multidisciplinary practices. Some techniques and research outcomes have already been incorporated as routine practices in biosecurity, while many others are yet to come. Many pheromones and other semiochemicals have been being used as highly sensitive, species-specific detection and surveillance tools. When different species interact each other, specific volatile chemicals are often produced. Detection of these specific signature volatile compounds can also be used as an early diagnostic tool. Highly sensitive and discriminating olfactory tissues can be used as low-level chemical sensors. Risk analysis is an important component of biosecurity program. The range of host plants may be predictable by using information on ORN response profiles of a given species and on volatile chemical profiles of the candidate plants. In a study using two Cydia species as a model system, we hypothesize that combinational input from two different groups of olfactory receptor neurons (ORNs), antagonist ORNs and agonist ORNs, may play a key role in discriminating host plants from non-host plants. Semiochemicals can be used as delimitation and eradication tools against invading species. For example, mass trapping with pheromone traps, combined with some other means such as sterile insect technique, effectively eradicated the painted apple moth, an invasive species, in New Zealand. A cooperative research program among New Zealand, USA and Australia is currently underway to use semiochemicals to eradicate the light brown apple moth invading California. Chemical ecology in aquatic environment has been poorly understood. Semiochemicals for marine organisms would also be useful to improve marine biosecurity.

벚나무응애의 기초적인 생활사와 발생실태를 조사하고, 과원에서의 벚나무응애 발생유무와 각종 살비제에 대한 방제효과를 조사하였다. 벚나무응애는 벚나무에서 4월 중순부터 11월 중순까지 발생하였으며, 6월 발생최성기를 나타내었다. 조사원 20개구의 관행복숭아원에서는 발생과원율이 35%로 나타났으나, 5개 지역 25개구의 관행사과원에서는 벚나무응애가 전혀 발견되지 않았다. 벚나무에서 월동처로의 이동시기는 10월 중순부터 11월 상순까지였고 활동개시기는 4월 상순이었다. 월동기간 동안 사충율은 약 54.9%정도였다. 각 처리온도구 16, 19, 22, 에서의 난기간은 각각 14.3, 9.2, 6.8, 4.0일이어고, 유충기간은 6.8, 5.0, 2.8, 2.3일, 전약충기간은 6.5, 4.2, 2.6, 2.0, 후약충기간은 6.9, 4.7, 3.3, 2.3일로서 고온일수록 발육기간인 단축되었다. 암컷성충의 수명은 갹 온도별로 각 67.7, 60.7, 46.4, 34.0일이었으며, 산란수는 각 21.2, 44.3, 54.4, 64.7개 였다. 발육영점과 유효적산온도는 난이 , 56,88일도, 유충은 , 28.76일도 전약충이 , 25.59일도, 그리고 후약충이 12.37, 29.3일도였다. 실내에서 성충에 대한 살비제별 방제효과 시험에서 아씨틴, 피리다벤, 프로지, 테부펜피라드, 펜피록시메이트 약제 처리구는 97.6%~100%로 우수한 방제효과를 나타내었으나, 치아스과 비스펜은 3.6%, 14.4%로 저조하였다. 난에 대한 방제시험에서는 무처리의 부화율 97.7%에 비해 방제가가 아씨틴이 84.1%로 다소 낮았고, 그외는 모두 98%이상으로 높았다. 난과 유충의 발육임계온도는 각각 평균 약와 였다.을 보였다. BT제로 도태한 개체군은 BT제에 대해서는 24배로 높은 저항성을 보였지만, 다른 계통의 약제에서는 1.1~2.0배로 비교차 저항성을 보였다. 생각되어진다.를 비롯한 다른 연구에서 학교급식에 종사하는 급식종사원의 급여 및 승진에 대한 만족도가 매우 낮게 나타났으므로 이에 대한 보다 적극적이고 체계적인 방안이 마련되어야 급식종사원은 급식에 대한 긍지와 자부를 느끼며 학교급식 업무를 효과적으로 수행하여 보다 질 좋은 급식을 아동들에게 제공할 수 있게 될 것으로 사려된다. 끝으로 본 연구는 대구 \ulcorner경북지역을 대상으로 한 연구이므로 전국을 대상으로 한 많은 연구가 계속되어 조리종사원의 직무만족에 관한 연구가 다양한 측면에서 이루어져야하겠다.지점의 특성에 따라 가장 경제적인 방법을 선택하는 것이 바람직하며, 특히 장비구매 설치 시 다양한 종류의 제품을 선택하는 것은 차후 장비 관리에 어려움을 겪을 소지가 있어 가능한 우수한 제품을 선택하되 동일 제품 사용을 권장한다. 따라서 위의 망구축이 이루어져 현재 기상청이 설치 운영하고 있는 측정 장비에 의해 취득한 기상자료를 공동 활용하여 표출하면 더욱 상세한 자료의 획득과 활용이 기대되어 진다. 또한, 금번 논문에서는 산불위험지역의 격자점(15km)내에 최소한 1대의 AWS 설치방안을 제시하였지만, 금후에는 15km내에서도 능선, 계곡 등 구체적인 위치확정을 위한 선행연구가 실시되어야할 것으로 판단된다.의해 원고는 결과발생지인 미국법원의 재판관할을 강제할 수 없을 것이다.

In this paper, the author gave a brief review on the meaning and background involving the growth of chemical ecology. Semichemicals which might be developed as insect control techniques incorporating in IPM program were described. The relevant semichemicals were grouped under separate topics including intraspecific semiochemicals, or pheromones (sex pheromones, alarm pheromones, and epidiectic pheromones), and interspecific semiochemicals, or allelochemics (allomones of Plant origin, and kairomones favoring natural enemies). Here, the author dealt with those of practical aspects only. The prospects of chemical ecology in insect pest management were also proposed.