The cotton aphid, Aphis gossypii (Glover), is one of the most serious pest in seed potato and various vegetable cultivation. The imidacloprid-resistant strain (IR) was over 200-fold more resistant to imidacloprid compared to a susceptible strain (S) as judged by LC50 values. The IR showed cross resistances to other neonicotinoid insecticides. IEF and 2DE analyses revealed that general esterase isozyme patterns in IR were almost identical to those of S. Nevertheless, a significantly overexpressed protein spot was detected in IR. To identify differentially expressed genes in IR, comparative transcriptome analyses based on GS-FLX were conducted using total RNAs extracted from both IR and S strains, which generated ca. 290 Mb reads for each strain. Generally, most nicotinic acetylcholine receptor subunit genes, such as alpha 2 and beta 1, were more transcribed in S than in IR. In contrast, only alpha 5 subunit gene was 1.8 fold more expressed in IR. Seven ATP-binding cassette (ABC) transporter genes were newly identified in A. gossypii, among which only ABCC9 gene was highly expressed in IR. Therefore, this ABCC subfamily, a member of the MRP subfamily which is involved in multi-drug resistance, could be one of the main factors associated with imidacloprid resistance in A. gossypii.

Biological control of Aphis gossypii by natural enemies was carried out in watermelon from April to June. Aphidius colemani was released 3 times at rate of 2/㎡ to control A. gossypii(April 24, May 8, and May 22 in 2007). In A. colemani-released plots, A. gossypii showed low population density of ＜ 0.6 per leaf with the highest parasitism of 57.2%, and 2.0~10.6% in the percentage of leaves with aphids. In control, the population of A. gossypii kept on increasing from early May and reached 653.2/leaf on may 29.

The cotton aphid, Aphis gossypii (Glover), is one of the most serious pest in seed potato and various vegetable cultivation. The imidacloprid resistant strain (IR) was over 200 fold more resistant to imidacloprid compared to the susceptible strain (S) as judged by the LC50 values and IR showed cross resistant to acetamiprid, thiamethoxam, thiacloprid, clothianidin. By using the suppression subtractive hybridization method, a imidacloprid resistant associated cDNA library was constructed in adult cotton aphid. In total 115 differentially expressed cDNA clones were obtained. Any point mutation detected in nicotinic acetylcholine receptor alpha 1~5 and beta 1 subunits in the IR. Based on IEF, the IR general esterase isozyme banding patterns were identical with that of S.

목화진딧물을 무궁화와 오이의 잎에서 개체사육하면서 생활사를 조사한 결과를 요약하면 다음과 같다. 1. 월동란은 4월중순하순에 부화하며, 부화율은 였다. 2. 5월하순6월상순에 유시충이 여름숙주로 이주하고 10월상순중순에 다시 겨울숙주로 이주하여 10월중순하순에 월동란을 낳았다. 3. 1년간 빠른 개체군은 22세대, 늦은 개체군은 6세대를 영위하였다. 4. 한세대의 성숙기간은 약 8일, 생식기간은 약 19일, 수명은 약 29일이었다. 5. 암컷한마리당 총산자수는 약 70마리, 최다산자수는 117마리 이었으며, 1일평균산자수는 3.7마리이고, 1일최다산자수는 17마리 이었다.