느타리버섯은 시중의 재배기술이 발전하여 느타리와 큰느타리의 전체 생산 및 소비가 버섯전체생산의 약 50%를 차지하여 재배품종의 주류를 형성하고 있다. 하지만 UPOV 및 FTA 등의 문호개방에 따라 로열티 지불 등에 대응할 수 있는 품종육성이나 신기술 개발이 시급한 실정이다. 본 연구에서는 느타리버섯의 성장단계별 유전적 발현을 분석하기 위해 cDNA 라이브러리를 성장단계별 10단계로 구분하여 12,342 ESTs를 구축하였다. 기존에 등록된 29,211 EST와 함께 분석한 결과 contig 4,939로 40%를 보여주었다. 이것을 기능별로 분류하였을 때 생물학적 과정 (Biological process) 27%, 세포 요소 (Cellular component) 35%, 분자 기능 (Molecular function) 38%로 분포하였다. 생물학적 과정에서는 생리학적 과정과 세포 과정이 각각 50%와 46%, 세포 요소에서는 세포와 세포부분이 각각 35%, 분자 기능에서는 촉매활성과 결합력이 각각 44%와 40%를 차지하였다. 유전자군별로 다양한 발현 패턴을 보여주었으며 조직특이적 발현은 약 13%를 나타내었다.

Perilla is a genus as a member of the mint family Lamiaceae which is known to contain lots of volatile metabolite. Perilla has been called as ‘deulkae’ indicating ‘wild sesame’ that means it has been maintained in Korea with long history. It has been very friendly used as edible oil and as fresh leaf vegetable. Perilla oil is valued for its medicinal benefit because it contains best amounts of unsaturated fatty acids, especially for the alpha-linolenic acid, known to omega-3 fatty acid, among all of the plant oils. It also include many beneficial phytochemicals. However, little study is conducted on their genetics. Here, we announce construction of well normalized and full length enriched-perilla cDNA library from a whole plant of one cultivar ‘Youngho-deulkae’ and their sequence characterization to provide useful resources for genetics, breeding and metabolite engineering. By sequencing of 5,760 cDNA clones, we 5,438 high quality EST sequences. Sequence trimming and assembly resulted 3,995 unigenes which consists 1,004 contigs and 2,991 singletones. Unigenes that showed little homology at the DNA sequence level with known genes in other plants even though they showed similarity at the protein domain level based on BLASTN, BLASTX, and TBLASTX. This study may provide good resources for initiation of further genomics, comparative genomics, functional genomics such as metabolic engineering and molecular breeding.

본 연구에서는 인삼 잎으로부터 정제한 mRNA를 이용하여 cDNA library를 제작하였다. 이 cDNA library로 부터 349개의 에너지 대사 관련 유전자를 선발 하였다. 에너지 대사 관련 유전자의 평균 사이즈는 0.49 kb이며, 에너지 관련 유전자들의 세부 기능별 발현을 분석한 결과 aerobic respiration(48.4%), accessory proteins of electron transport and membrane associated energy conservation(17.2%), glycolysis and gluconeogenesis(3.4%), electron transport and membrane associated energy conservation(2.9%), respiration(2.0%), glycolysis methylglyoxal byp-ass(1.7%), metabolism of energy reserves(0.6%)와 alcohol fermentation(0.3%)의 분포를 보였다. 인삼 잎에서 발현되는 유전자중 가장 많이 발현된 Chlorophyll a/b binding protein of IhcII type I(36.6%), Photosystem II oxygen-evolving complex protein(6.6%) 등이 발현되었다.

Hairy roots were induced from Korean ginseng (Panax ginseng C. A. Meyer) root explants and studied for their gene expression. A total of 3,000 ESTs (expressed sequence tags) from ginseng hairy root were determined and about 2,700 ESTs have a length of readable sequence, which result in 1,352 unique ESTs sequences. The 879 ESTs showed significant similarities to known nucleotide or amino acid sequences in other plant species, which were divided into eleven categories depending upon gene function. The remaining 473 sequences showed no significant matches, which are likely to be transcripts or to be matched to other organisms. The results indicated that the analysis of the ginseng hairy root ESTs by partial sequencing of random cDNA clones may be an efficient approach to isolate genes that are functional in ginseng root in a large scale. Our extensive EST analysis of genes expressed in ginseng hairy root not only contributes to the understanding of the dynamics of genome expression patterns in root organ but also adds data to the repertoire of all genomic genes.