The anti-inflammatory effects of the glycosaminoglycan (GAG) derived from cricket (G. bimaculatus, Gb) were investigated in complete Freund’s adjuvant (CFA) treated chronic arthritis rat model. This GAG produced a meaningful anti-edema effect showing inhibition of C-reactive protein (CRP) and rheumatoid factor. This GAG also inhibited the atherogenesis and pro-inflammatory cytokine levels of VEGF production in HUVEC cells, IL-6, prostaglandin E2 stimulated lipopolysaccharide in LAW 264.7 cells and TNF-α production in normal splenocytes, with dose dependent manner. This GAG was also found to be an inducer of NO production from the HUVEC cells and a stimulator of endothelial nitric oxide synthase. In the histological finding, the LV dorsal root ganglion, linked to the paw treated Gb GAG, was repaired against CFA induced cartilage destruction. The combined Indomethacin (5 mg/kg)-Gb GAG (10 mg/kg) also more effectively inhibited CFA-induced paw edema at 3h, 2nd and 3rd day to levels comparable to anti-inflammatory drug, indomethacin.

rt is well known that glycosaminoglycans are 01' fllndamental importance to the processes 01' morphogenesis and cytodifferentiati on dllring the teeth development , With HlD-TCH-SP(High - iron diamine-thiocarbohydrazide-silvel protcinntc) , s lllfatcd glycosaminoglycans such as chondroitin sulfate and heparan slllfate have been localized a t the III trastrllctural level i n a wide variety of tlssues The pmpose of this stlldy were to examine slllfated glycosaminoglycan at llltrastrllctllral level fo 1' the phase of morphogenesis and cytodifferentiation of hllma n fetal tooth germs, and to detect the protein expression of slllfated glycosaminoglycan by immunoslot blot Human tooth gerll1s fl'Oll1 the a lveolar bone of twenty still born fetuses we1'e fixed in a mixtllre of 2% gllltaraldehyde/1% forma ldehyde, The 미 t 1'athin sections were stained witb HID-TCH-SP and were treated with 0, 05% solution of t esticular hyaluronidase to identify the histochemical properties 01' tbe HID-TCH-SP stain deposits , For semi quantitative protein assay , immllnoslot blot was done Sulfated glycocongugated deposits were localized in DEJ, peri tubular dentin , and mantle dentin matrix‘ enamel prism sheath , interrod area , and enamel matnx Heparan sll l띠 te deposits i n DEJ resisted to testicular hyalllronidase treatment prior to HID-TCH-SP staining, Immunoslot blot s howed that• chondroitin sulfate was detected higher in enamel and dentin extract, while heparan sulfate was relatively expressed in enamel and dentin extract, but rarely expressed in enamel or dentin extract It suggest ecl that chonclroi tin and hepa ran slllfate woulcl play an important role in the formation of DEJ, while chondroitin sulfate would in the clevelopll1ent of enamel prism sheath, enamel matrix, and mantle 01' peritublllar clentin of human fetal tooth germs,

It is well known that glycoproteins, glycosaminoglycans and proteoglycans are of fllndamental importance to the processes of morphogenesis and cytodifferentiation dllring the teeth development. With HID-TCH-SP(High-iron diamine-thiocarbohydrazide-silver proteinate), slllfated glycosaminoglycans sllch as chondroitin slllfate and heparan slllfate have been localized at the 1I1trastructurallevel in a wide variety of tisslles. 까le pllrpose of this study were to characterize slllfated glycosaminoglycan profiles of hllman fetal tooth genns at 비trastructurallevel for the phase of morphogenesis and cytodifferentiation, and to detect the protein expression of sulfated glycosaminoglycan by immunoslot blot. Human tooth germs from the alveolar bone of twenty still born fetuses were f1xed in a mixture of 2% glutaraldehyde/ l% fonnaldehyde. The ultrathin sections were stained with HIDTCH- SP, and some sections were σ'eated with 0.05% solution of testicular hyaluronidase to identify the histochemical properties of the HID-TCH-SP stain deposits. For semiquantitative protein assay, immunoslot blot was done. The obtained results were as follows 1. HID-TCH-SP staining showed sulfated glycocongugated deposits in DEJ, peritublllar dentin, and mantle dentin matrix, enamel prism sheath, interrod area, and enamel matrix. 2. Heparan sulfate deposits in DEJ resisted to testicular hyalllronidase treatment prior to HID-TCH-SP staining 3. In immunoslot blot, chondroitin slllfate was detected higher in enamel and dentin extraα ， while heparan slllfate was relatively expressed in enamel and dentin extract, but rarely expressed in enamel or dentin extract. From the aboving results, it was suggested that chondroitin and heparan sulfate would play an important role in the formation of D티， while chondroitin sulfate would in the development of enamel prism sheath, enamel matrix, and mantle or peritllblllar dentin of human fetal t∞th germs.