The aim of this study was to evaluate the biological mechanism of orally administered fly maggot ethanol extracts (EM) on hypocholesterolemic in rats fed a high-cholesterol diet. SDS male rats were divided into four groups (EM dose control=0, 5.0, 7.0, and 9.0 mg/100 g BW) and were treated for 6 weeks. EM groups revealed a significant reduction in serum triglyceride, total cholesterol, and low-density lipoprotein-cholesterol when compared with the control group. HMG-CoA reductase activity in EM groups were significantly lower than those of the control group, but total sterol, neutral sterol, and bile acid excretion were significantly increased in EM groups when compared with the control group. It was discovered that EM suppress the expression of SREBP-1⍺ and SREBP-2 mRNA in the liver tissues of high-cholesterol diet fed rats, while simultaneously increasing the expression of PPAR⍺ mRNA. This finding indicates that EM has a hypocholesterolemic effects in rats fed a high-cholesterol diet, by regulating cholesterol metabolism-related biochemical parameters and SREBP-1⍺ SREPB-2 and PPAR⍺ gene expression.