1. 미소 해충의 자동 영상 인지 시스템 개발에 필요한 기초 자료를 확보하기 위해 화질에 따른 해충의 인지율을 조사하였다. 2. 곤충 영상은 스캐너를 이용하여 300, 600, 900, 1,200, 2,400, 4,800 dpi의 6단계 화질로 획득하였고 곤충 연구자에게 화질에 따른 인지율을 설문 조사하였다. 3. 나방류인 담배거세미나방과 배추좀나방의 경우 모든 dpi 에서 높은 인지율을 보였다. 4. 크기가 작은 포인세티아총채벌레와 담배가루이의 경우 모든 dpi에서 50% 미만의 낮은 인지율을 보였다. 5. 크기가 작은 총채벌레와 담배가루이 인지 시스템 개발에는 100 mm 매크로렌즈를 사용하거나 hand-held 형태의 디지털 현미경이 필요할 것으로 사료된다.

In this study, we isolated and identified an aggregation-sex pheromone from Monochamus saltuarius, the major insectvector of the pine wood nematode in Korea. Adult male of M. saltuarius produces 2-undecyloxy-1-ethanol, which is knownto be an aggregation-sex pheromone in other Monochamus species. We performed field experiments to determine the attractivenessof the pheromone and other synergists. More M. saltuarius adult beetles were attracted to traps baited with the pheromonethan to unbaited traps. Ethanol and (-)-α-pinene interacted synergistically with the pheromone. Traps baited with pheromone+(-)-α-pinene+ethanol were more attractive to M. saltuarius adults than traps baited with pheromone, (-)-α-pinene, or ethanol alone.Ipsenol, ipsdienol, and limonene were also identified as synergists of the aggregation-sex pheromone for M. saltuarius adults.

Allomyrina dichotoma is a typical pet beetles in Korea. From 2010, similar symptoms of milky spore disease were found collectively in grubs of the species reared in insect farms. They shared a specific symptom that the skin of last instar larvae was changed softer with opaque white and infested grubs eventually died. To clarify the cause of the symptom, we collected the larvae of A. dichotoma from five farms and examined intestinal bacterial florae of them using pyrosequencing technique. From those results, a member of Paenibacillus was found only in the larvae showing the symptom of disease. Through PCR analysis using a Paenibacillus specific primer set, we obtained the partial 16S rRNA gene sequence and confirmed the microbe as Paenibacillus sp. For detailed characterizing, a whole guts was extracted from each larva showing the sign of the disease and incubated at 70℃ for 15 min to isolate spore forming bacteria. After then, each content of guts was cultured on MYPGPNAL agar medium (12.5 μg/ml of nalidixic acid) at 30℃. The 16S rRNA gene sequence analysis for six isolate showed that they were closely related to P. rigui (97.5~98.0% similarity) and to P. chinjuensis (95.9~96.6% similarity). Additional tests including API test and cellular fatty acid composition analysis were performed, but the strain couldn’t be identified at species level, suggesting it may represent novel species of the genus Paenibacillus.

Due to their ease of sequencing and high levels of nucleotide diversity mitochondrial (mt) genes have long been favoured targets for investigations of species level issues in systematic entomology particularly species delimitation and diagnostic identification. Advances in sequencing technology and price reductions have now made routine sequencing of whole insect mt genomes feasible and thus the application of much larger datasets to these perennial problems can be attempted. I will present two examples – the development of diagnostic targets within forensically important blowflies (Diptera: Calliphoridae) from scans of whole mt genome variability and the determination of species status within the cotton bollworm (Lepidoptera: Noctuiidae: Helicoverpa) – to illustrate the ease and comparative cheapness of genomics level approaches to these age old problems.