KAS360-22 종자에 1998년 250 Gy의 감마선을 조사하였다. 그 후대에서 미이라병에 저항성을 가지는 계통을 선발･육성하여, ‘원율’로 명명하고 2009년에 최종적으로 품종보호권을 출원 신청하여 2012년 등록 완료하였다. 원품종 KAS360-22의 종피색이 노란색인데 반하여 신품종 ‘원율’은 갈색을 나타냈다. 100립중은 재래종 KAS360-22이 17.2 g, ‘원율’은 27.5 g으로 종자무게는 증가하였으며, ‘일품검정콩’(27.4g)과는 별다른 차이를 보이지 않았다. 신품종 ‘원율’의 10a 당 수량은 미이라병 발병 조건에서 233.3 kg으로 재래종 KAS360-22 (24.9 kg)에 비교하여 10.6배 가량 높았고, 대조구 ‘일품검정콩’(121.4 kg)에 비교하여도 1.9배 가량 높아 밥밑용 콩으로 안정적인 수량 확보가 가능할 것으로 사료된다.

NGS costs are decreasing rapidly, and beneficial application of the technology to plant genomics seems inevitable. Trying to interpret the agriculturally important traits like yield is actively in progress all across the globe. However, the current stage of bio-informatic technology as applied to the interpretation of agricultural trait appears not yet at a level of maturity to justify widespread plant genome sequencing for user-friendly molecular breeding. It is necessary to construct dense mutation block (DMB) based molecular breeding system for selecting plants with optimal agricultural performance; as well as for identifying useful quantitative trait loci (QTLs). Firstly, we screened and selected DMBs-specific INDEL markers obtained from SNV density profiles using 42 genome sequences of Korean cultivar and public sequences of 24 japonica rice cultivars. Secondly, we analyzed the genetic similarity between 288 Korean cultivars using 113 DMB-specific INDEL markers, which could differentiate on agarose gel by PCR. And we are going to integrate 360 INDEL markers up to 30 per each chromosome. Finally, we selected 40 founder lines considering the importance of the breeding, the purpose of use, and plant ecotype. To construct rice nested association mapping population we crossed each founder lines with the pollen of Hwayoungbyeo which was most commonly used in korean rice breeding program. F2 seed multiplication and generation iteration are ongoing.

A 41-year-old woman arrived at our emergency department with semicomatous mentality due to aborted cardiac arrest from ventricular fibrillation, which occurred during resting state. Prolonged QT interval in condition of hypokalemia and hypomagnesemia was observed on initial electrocardiography. However, even after normalization of serum potassium, magnesium and mentality, QT prolongation was sustained. In addition, genetic analysis showed KCNH2 mutation which was consistent with type 2 congenital long QT syndrome (LQTS). In this case, the patient was treated with an implantable cardioverter defibrillator and genotype specific medical therapy with potassium channel opener.

The world is facing a serious food and energy crisis. Plant mutation breeding has played an important role in overcoming this crisis and maintaining world stability. New techniques are required to achieve faster and more effective breeding. At RIKEN, we have developed a unique technology for mutation induction by using heavy-ion beams from particle accelerators at the RI Beam Factory. This development was achieved through an efficient synergistic link between agricultural science and accelerator physics. The use of ion beams for mutagenesis has a number of advantages: the approach has low exposure levels and high survival rates with high mutation rates, and it creates a wide variety of different mutations. Because heavy-ion beams provide a very high amount of energy, even a single ion is enough to significantly damage a gene – in fact, the beams have enough energy to break the double strand of the DNA. The technique is also very useful in producing mutants that lack just a single gene; multiple propagation technology can be used to convert these mutants into new cultivars. Examples of such breeds include ‘Safinia Rose’ (petunia), ‘Temari Bright Pink’ (vervena) and ‘Olivia Pure White’ (dianthus). The development period for producing new varieties is only 2 years. Over last decade, molecular biology has made great advancements through technological innovation. We use high-throughput DNA sequencing techniques such as next-generation sequence instruments and microarray technologies for analysis of gene mutations. Mutants have become more and more useful and important in modern genetic studies, enabling the discovery of genes that control important traits, and revealing the functions and mechanisms underlying their operations. The discovery of genes using mutants may lead to the emergence of a new field in biology, ‘Mutagenomics’.

재래종 서리태는 밥밑용 콩으로 한국인에게 선호되고 있으나, 숙기가 느려 수량성이 떨어지고 콩알이 커서 청소년들에게는 기피 경향이 있다. 서리태의 특성을 개량하고자 1994년 수집한 서리태 종자에 감마선 250 Gy를 조사하여 돌연변이 육종을 시도하였다. 그 후대에서 숙기가 빠르고 수량성이 높으며 콩알 크기가 작아진 유망 돌연변이 계통을 선발하여 ‘조생서리’로 명명하고, 2005년도에 품종보호권 및 국가품종목록등재 신청을 하였으며, 2008년도에 등록을

Recently, giant embryonic rice and functional rice food are preferred by more consumers, which are attributed to the fact that the embryo has high concentrations of essential amino acids, fatty acids, and vitamins relative to other parts of rice grains. In this report, the heredity and stability of giant embryo mutations in successive generations were analyzed regarding a giant embryonic line, 'P47', induced by T-DNA insertion and a F2 population from a cross between 'P47' and 'Junam'. The mutant lines with increases of 1.5, 1.7 and 1.8 times on embryo length, width and 100-embryo weight to those of the control showed stable inheritance across three generations. The continuous frequency distributions of embryo size in the F2 population showed that the embryo size is a quantitative trait of polygene controlled. In addition, wide range of transgressive segregations of six traits affecting embryo size confirmed exchange of genetic materials and recombination between genes controlling embryo size. Five giant embryo mutant lines selected from the F2 population will be used for artificial selection and improvement of giant embryonic varieties.

Encouraging progress in the mutation breeding has been achieved since a mutation induction has become increasingly important for a cultivar development for complex reproductive or propagating modes, the creation of new genetic resources, especially for crops with a narrow genetic base, and the use of mutants for a genomics study. In Korea, more than 35 cultivars have been released by using the mutation breeding method since the mid-1960s, and the released cultivars were mostly developed (76%) by exposing to radiations (gamma and/or X-ray). Most of the released mutant cultivars (74%) in Korea were food and oil seed crops, especially for improving agronomic traits such as yield, lodging tolerance, maturity, or functional compounds. Currently a high yield potential of cultivated crop cultivars is relatively less popular than before, but the expectation of value-added crops, from the farmer’s side, is in high demand. Accordingly, the mutation breeding program in Korea has assigned more resources to other crop species, including some flowering and ornamental plants. These flowering and ornamental plants are ideal systems for a mutation breeding because their favored traits such as flower color or shape or plant architecture can be visually monitored after a mutagenic treatment. Additionally, these plant species are genetically heterozygous and often propagated vegetatively, which allows for an isolation and selection of mutants within M1 generation. In Korea, a program for the development of potential cultivars of flowering and ornamental crops was launched with financial support from the Biogreen 21 project in RDA. Thisintegrated program which will be conducted by a diverse array of experts will focus on major flowering and ornamental crops in Korea such as rose, chrysanthemum, lily, carnation, orchids, and clover. The potential outcomes from the program will be new highly valued-added cultivars which will provide greater money gains to Korean farmers and lots of valued mutants used for a gene isolation of interest and reverse genetics or functional genomics. Appropriate strategies should be implemented to complete its goals successfully, which includes a)induction of a wide mutant spectrum, b)applications of new irradiation techniques, c)unraveling the complex genetic phenomena controlling mutant traits, and d)development of a mass production and an intensive export system for the developed cultivars.

"changhae" is a new Hibiseus variety developed by a mutation breeding by using a gamma ray irradiation. O dred seeds ofthe or iginal variety, "Suminokura", grown in a breeding field in the Namyangju, Gyeonggi Province were irradiatedwith a 100 Gy gamma ra

Ggoma' is a new Hibiscus variety released by a mutation breeding using a gamma ray irradiation at the Korea AtomicEnergy Research Institute (KAERI). One hundred seeds ofthe origmal native variety, 'Hongdansim 2', were collected from arounda 35 year old pl