Grifola frondosa has been used as an herbal medicine for the treatment of cancer, diabetes mellitus and high blood pressure. In this study, functional polysaccharide was obtained from Grifola frondosa using four different extraction methods: hot water(HwFP), homogenize(HgFP), acid(AcFP), and alkali(AlFP) extraction methods. The effects of these extracts on KB and HepG2 cell lines were then examined for any anti-cancer activity. Alkaline extraction produced a yield of 0.175% and the total sugar content of the extract was 54.97%. We were able to confirm that the polysaccharide extracts from the mushroom produce an anti-cancer effect. The cytotoxicity of AlFP and AcFP against HepG2 cells were 22.86% and 28.88%, respectively, and the cytotoxicity of AlFP against the KB cell lines was 47.76% at a concentration of 1, 000 ㎍/㎖. Therefore, these results suggest that the optimum method for extracting functional polysaccharides from G. frondosa is the alkali extraction method.
매화의 품종과 건조방법에 따른 향기성분을 분석하여 매화차 제조에 맞는 재료와 처리방법을 찾고, 매화의 첨가량 및 침출 온도, 녹차 첨가 비율 등을 관능검사를 통하여 살펴본 결과, 매화의 휘발성 향기성분의 분석결과 총 17종이 검출되었으며 그 중 생화의 경우 총 15종이 검출되었다. 동결건조 시료에서는 건조과정중 1가지 성분이 소실되었고 1가지 성분이 다르게 검출되었으며, 음건의 경우4가지 성분이 소실되었고 1가지 성분이 다르게 검출되었다. 3가지