Grifola frondosa has been used as an herbal medicine for the treatment of cancer, diabetes mellitus and high blood pressure. In this study, functional polysaccharide was obtained from Grifola frondosa using four different extraction methods: hot water(HwFP), homogenize(HgFP), acid(AcFP), and alkali(AlFP) extraction methods. The effects of these extracts on KB and HepG2 cell lines were then examined for any anti-cancer activity. Alkaline extraction produced a yield of 0.175% and the total sugar content of the extract was 54.97%. We were able to confirm that the polysaccharide extracts from the mushroom produce an anti-cancer effect. The cytotoxicity of AlFP and AcFP against HepG2 cells were 22.86% and 28.88%, respectively, and the cytotoxicity of AlFP against the KB cell lines was 47.76% at a concentration of 1, 000 ㎍/㎖. Therefore, these results suggest that the optimum method for extracting functional polysaccharides from G. frondosa is the alkali extraction method.
한국에서 주로 섭취되고 있는 주요 식이섬유질원으로서 장내 유익균의 생육을 촉진한다고 알려진 사과, 미역, 쑥, 머위를 천연 형태로 건조분말화하여 식이내에 5%, 15%가 되게 첨가한 후 노화가 진행된 흰쥐에게 4주간 급여하였다. 그 결과 β-glucuronidase는 셀를로스군과 미역 15% 첨가군에서 가장 낮은 활성을 나타냈으며 β-glucuronidase의 경우는 쑥 15% 첨가군에서 유의적으로 현저한 증가를 보였다. 부패산물인 인돌 함량 변화는 쑥 섭취군에서 현저하게 낮은 경향을 보여 β-glucuronidase와는 다른 양상을 보였다. 수분함량은 미역섭취군과 쑥5%, 머위15% 섭취군에서 높았으며 pH는 쑥 15% 섭취군이 가장 낮은 값을 보여주었다. 쑥 15% 섭취군이 장내균총 조성을 개선시킬 수 있을 것으로 보고되어 그 유의성을 밝히고자 하였으나 본 실험에서는 부패산물, 유해효소등과 장내균총의 조성과의 뚜렷한 관계는 발견하지 못하였다.
In order to investigate the extraction mechanism of barley tea in cylindrical packed column, pilot plant scale packed column was designed and constructed. And extraction conditions for steady flow in the packed column were searched. The main results of this study are as follows : 1. Circulation of the stock barley tea before the extraction running was indispensable in consideration of inequality, gas, particles existed in packed bed. Solid-liquid equilibrium was initially maintained after the circulation. 2. Flow direction of solvent must be up-flow for maintaining the constant bed height and flow rate during the extraction. Paessure drop in pucked bed was similar to decrease ratio of extract concentration. 3. The porosity of packed bed was in the range of 0.24∼0.36 according to the particle size. And it was decreased as the particle size became smaller.