Viral protein 2 (VP2) of porcine parvovirus (PPV) is responsible for inducing neutralizing antibodies in immunized animals. It is the major viral structural protein. In this study, novel subunit vaccines against PPV based on virus-like particles (VLPs) formed from VP2 proteins (PPV 13-7 Korean strain) were expressed in an insect baculovirus cell system and purified using Ni-NTA affinity column chromatography. These VP2 proteins assembled into virus-like particles (VLPs). They showed antigenic properties similar to those of natural PPV. In addition, they showed high hemagglutination (HA) titers (211 for PPV 13-7 Korean strain). This study provides a foundation for the application of the difference immunization of recombinant protein in the diversity of PPV VP2 genes and in vaccination against PPV in the future.

A field experiment was conducted to evaluate growth characteristics, forage production and crude protein yield according to cutting time of Soghum Sudangrass Hybrid, and decide ideal harvesting time for use of soiling and silage. Experiment design was arr

본 시험은 alfalfa파종시 겉뿌림 및 다른 파종방법들이 alfalfa의 생육. 건물수량.조단백질 생산량 및 잡초발생에 미치는 영향을 구명하고자 겉뿌림, 겉뿌림+갈퀴질, 겉뿌림+갈퀴질+쇄압, 경운+산파+쇄압 및 경운+조파+쇄 등 5개 처매구를 두고, 1988년 9월부터 1989년 10월까지 축산시험장 초지시험포에서 수행되였으며, 결과를 요약하면 다음과 같다. 1.경운방법으로 6개월후에 토양산도와 토양의 매화학적 특성은 개선되었다. 2 알팔파의 정착개체

This field experiment was carried out to determine the effects of fertilizer application time in spring on the growth, crude protein(CP) content and dry matter(DM) yield of grasses for soiling. Application times of fertilizer were March 10, 20, 30, April

This field experiment was carried out to determine the effects of fertilizer application time in spring on the growth, crude protein (CP) content and dry matter (DM) yield of gasses for grazing Application times of fertilizer were on March 10, March 20, M

The present study was conducted to investigate effect of dietary protected amino acid on milk yield and composition in dairy cow using meta-analysis. Total 21 research papers were employed in analysis, and mixed model was used for the analysis of effects. Effect of protected methionine (PM) and combination of protected methionine and lysine (PML) were investigated under two different levels of dietary crude protein (CP, <18% and >18%). For performance of dairy cow, milk yield, milk composition including milk fat and protein content and yield and 4% FCM (fat corrected milk) production were used for analysis. In case of milk yield, a trend of increment was found at PM supplementation at low CP (P=0.055). However, the effect of PM at high CP was detected as not significant (P>0.05). In case of milk protein, inclusion of PM at low CP showed significant decrement (P<0.05). However, there was no significant effect of MP on milk protein at high CP (P>0.05). Supplementation of MP at high CP level showed significant increment of milk fat (P<0.05). MP supplementation represented significant increment of 4% FCM production (P<0.05) regardless of dietary CP levels. Effects of PML on milk yield and composition at both of low and high dietary CP were not significant in this study. However, it seem to be that there was a possible positive effect of MPL application at high dietary CP on performance of dairy cow.

Rice as a typical silicified plants needs the intemal absorption of silica, especially during the growing period. The leaves and culm of plant are erected up by uptake of silicic acid. Sun light could reach the bottom leaves in the plant communities. In addition, the nitrogen uptake would be physiologically controlled by the application of silica and the quality of rice could be improved by reducing the quantity of protein in grian. In the field experiment designed, the rice cultivar was DonhjinⅠho, Daeanbyeo and transplanting day was May 25, 2007. N levels per ha were 100 and 200kg, respectively and liquid silica(ai 11%, manufactureed by Dongbuhiteck Co.) was sprayed on flag leaves according to 500fold and 1000fold with distilled water and 3 times at an interval of 1week from 30 days before heading. The rice culms treated with each liquid silica levels were shortened and panicle length were increase as compare with the control in the level of each N. The lodging was reduced in the plot that treated with the higher liquid silica application of 100kg as compared with 200kg plot in the filed. The rice yields of the plots treated with silica application were more increased than in the control plot. All yield components, except of panicle number in DongjinⅠ, increased as compare with 200kg plot in the filed. Changes measured by 2-DE in display expression of flag leaves and seed in relation to nitrogen treatment level and silica trements were similar in fashion, yet still displayed distinguishable differences in display strength spots.

Randomized field experiments were conducted to study the interactive effect of sulphur (S) and nitrogen (N) on seed, oil and protein yield of two cultivars of groundnut Arachis hypogea: cv Amber (V1) : cv Kaushal, (V2) . Two dosage levels of sulphur (0~;and~;20kg~;ha-1 ) and two dosage levels of N (23.5~;and~;43.5kg~;ha-1 ) in various combinations were tested as micronutrient treatments, T1,~;T2,~;and~;T3 . Results indicated significant enhancement of the yield components namely seed and oil yield as well as seed protein. Maximum response was observed with treatment T3 (having 20kg S and 43.5kg N ha-1) . Increase in seed and oil yields of 90% and 103% in V1 , and 79 and 90% in V2 , respectively were recorded as compared to the control treatment T1 (having 0kg S and 23.5kg N ha-1 ). Effect of S and N interaction was observed on protein, N and S content in seeds. The results obtained by these experiments clearly suggest that judicious balanced application of N and S could improve the yield.

Response of grain yield and milled-rice protein content to nitrogen (N) rates at various growth stages is critical for quantifying real-time and real-amount of applied N requirement for target grain yield and protein content. An experiment including 10 N rate treatments at transplanting, tillering and panicle initiation stages with four rice cultivars in 2003, 6 N treatments with two rice cultivars in 2004 and 2005 was conducted. Increase of N rates at PIS significantly increased both grain yield and milled-rice protein content but increase of N rates at tillering stage significantly increased grain yield but not milledrice protein content. Therefore, high grain yield and low milled-rice protein content would be difficult to obtain only by adjusting N rates at PIS. Internal N use efficiency (INUE) was 60.5 kg grain/kg N accumulation on an average over N treatments, cultivars, and experimental years, showing considerable reduction especially at high shoot N accumulation in the experimental year of low sunshine duration. Milled-rice protein content tended to increase almost linearly with increasing shoot N accumulation, but it revealed big variation even at the same shoot N accumulation at harvest. Milled-rice protein content decreased with increasing INUE. N accumulation in the milled rice increased at an almost constant proportion of 45.5 percent of the shoot N accumulated at harvest, showing slight decresing proportion with the increasing shoot N accumulation.

Response of grain yield and milled-rice protein content to nitrogen topdress (N) timing at panicle initiation stage (PIS) is critical for quantifying real-time N requirement for target grain yield and milled-rice protein content. Two split-split-plot experiments with three replications, one in 2004 and the other in 2005, were conducted in Experimental Farm, Seoul National University, Suwon, Korea. The experiments included three N rates at tillering stage (TS), three N timing treatments at panicle initiation stage (PIS) and two rice cultivars. The N rates at TS, N timing at PIS, and rice cultivars were randomly assigned to main plot, sub plot, and sub-sub plot, respectively. Results showed that the delayed N application at PIS reduced grain yield in 2004 and increased milled-rice protein content in both years significantly at 0.05 probability level. The calculated optimum N timing at PIS from pooled data by N rates and rice cultivars in two years was at 28 days before heading (DBH). However, real-time of N timing at PIS was dependent on plant growth and N status around PIS that in turn was dependent on applied N rates at TS. The optimum N timing at PIS was at 30 DBH for no N treatments at TS while at 27 DBH for 3.6 and 7.2 kg N/10a treatments and at 27 and 29 DBH for Hwaseongbyeo and Daeanbyeo, respectively. In general, earlier applied N at PIS resulted in lower milled-rice protein content but the highest grain yield was expected to be obtained when N topdress at PIS was applied at the time when shoot N concentration started to drop below about 23 mg/g due to dilution effect after transplanting. In conclusion, the results of our experiments imply that the currently recommended N topdress time (24DBH) at PIS in Korea should be reconsidered for the higher grain yield and the better quality of rice.

The effects of Rhizobium inoculant, nitrogen, phosphorus, and molybdenum on nodulation, dry matter production, yield attributes, pod and seed yields, protein and phosphorus contents in seed of pea (pisum sativum) var. IPSA Motorshuti-3 were assessed by a field experiment. Among the treatments Rhizobium inoculant in combination with 25kg P and 1.5kg Mo/ha performed best in recording number of nodules/plant, total dry matter yield, number of pods/plant, number of seeds/pod, 1000-seed weight, green pod yield, green and mature seed yields of pea. The highest green pod yield of 15.37 t/ha (97.05~% increase over control) and green seed yield of 9.6t/ha (69.31~% increase over control) were obtained by inoculating pea with Rhizobium inoculant in association with 25kg P and 1.5 Mo/ha. The effects of 60 or 120kg N/ha were comparable to Rhizobium inoculant in most cases. There were positive correlations among yield attributes, yield, protein and phosphorus contents in seeds of pea. From the viewpoint of yield attributes, yield, and seed quality, application of Rhizobium inoculant along with 25kg P and 1.5kg Mo/ha was considered to be the balanced combination of nutrients for achieving the maximum output from cultivation of pea in Shallow-Red Brown Terrace Soil of Bangladesh.

1. 경장 경경 주경절수는 5월 15일 파종에서 파종후 70일경에 거의 완성되었으며 파종시기가 20일 40일로 지연되므로서 완성에 이르는 시기가 10, 20일식 빨랐고 품종간에서는 충북백이 가장 빨랐다. 2. 협수는 5월 15일 파종에서 가장 많았고 분지협이 주경협보다 많았으며 6월 25일파에서는 주경협이 많았다. 분지협에서는 공협과 1입중간에서 충북백은 2,3입협율이 높았고 김두와 장단백일은 1,2입협율이 높았으며 익산은 1,3입협율이 높았다. 3. 주경절위별 착입수가 3-4절에서 가장 많았고 9-10절이 다음으로 많았다. 4. 만파에서는 수량이 떨어지며 5월 15일파에 비하여 6월 5일파와 6월 25일파의 감수율은 충북백 6% 14% 익산 9% 17% 김두 15% 26% 장단백일 18% 27%였다. 5. 단백질함량은 5월 15일 파종한 것이 가장 높았고 유지함량은 6월 25일 파종한 것이 높았으며 품종간에서 단백질은 장단백일이 유지는 익산이 가장 높았다.