The purpose of this study was to determine the free amino acid contents, total phenolic contents and antioxidant activity in raw Sesamum indicum seeds (cv. Kopum and cv. Mihuk) and their sprouts germinated for 7 days. Total free amino acid contents of sprouts (29.34±3.3 mg/g DW) were significantly higher than that of raw seeds (6.85±0.39 mg/g DW). All individual free amino acid, including asparagine, alanin, arginine, and leicine were significantly increased in the sesame sprout. And also germinated sprout significantly increased the total phenolic contents (2.2±0.3 mg GAE/g) that resulted in the increased DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging capacity. Subsequently, two varieties of sesame and its sprouts were analyzed for their phenolic constituents using high-performance liquid chromatography (HPLC). Catechin, sinapic acid and salicylic acid were identified as the major phenolic acid presented in sesame sprout. However, the major biological constituents sesamin and sesamolin content were significantly decreased during germination.

Sesame (Sesamum indicum L.) is one of the most important oilseed crops, having seeds and its edible oil that are highly valued as a traditional food. Many studies have examined the health value of sesame seeds and oil. Among the bioactive components in sesame seeds, lignans and tocopherols have been identified as the major antioxidants responsible for the resistant oxidative deterioration of sesame seeds and oil. These same antioxidants have been reported to have protective effects against human disease such as neurodegenerative disease. This review summarizes the chemical properties of lignans including lipid-soluble lignans, lignan glucosides and tocopherols, and their bioactivities such as antioxidativity and neuroprotection. We also review the biosynthesis of lignan in sesame seeds and transformation of lignans during food processing in sesame oil. Sesame seeds and its antioxidants may be a potent natural agent with both therapeutic applications and use in preventing human illness such as neurodegenerative disease.

This experiment was conducted to develope optimum cultural system of sesame under greenhouse condition with the comparison of open field cultural condition. The relatively higher adaptable varieties under greenhouse condition were white sesame "Pyoungan" and black sesame "Jinki" which showed higher yield potential and disease resistance. To set up standardized cultural system under greenhouse condition, we conducted several experiments of optimum sowing date, planting density and pinching time etc. When it came to sow sesame seed soon after early cultivated crops were harvested, the yield potential was higher than those of other sowing dates. Therefore, "June 30th" was optimum sesame sowing date in the greenhouse cultivation system. We also found out the space of 40㎝ between rows and 30cm(a few branch type) or 40cm(many branch type) between plants was better than those of other treatments. Optimum pinching time was 25 days after first flowering date when 1000 seeds weight and yield potential showed higher than other treatments.

This experiment was conducted to develope optimum seedling quality for the sesame transplanting system. To determine optimum seedling quality condition, we conducted several experiments. At the optimum pot selection experiment for the sesame transplanting, plastic pot with 105 holes showed relatively better early growth status of sesame than those of other plastic pots with 72, 128, 164 and 200 holes. And the soil combination, 'Bunong + Plant World' which is mixed 50% respectively showed higher germination rate, lower disease and lodging rate than those of other nursery soils at the determination of optimum nursery soil mixtures. We also conducted one experiment to find out seedling duration for the optimum transplanting seedling quality. According to the result, the seedling stage with about 4 or 5 regular leaves which came to 40 days after sowing showed higher number of capsule per plant and yield potential. Those results will help to develop mechanized sesame seedling transplanting system to reduce labor and increase net income for the sesame cultivation farmers.

This experiment was conducted to find out optimum priming treatment conditions to the sesame seed as a preliminary study for enhancing sesame germination properties. Effective priming agents and concentrations for sesame seed were K3PO4 , 200 mM and PEG6000, -1.0 MPa respectively. Optimum priming temperature and duration were 15~circC , 4 days in view of germination speed and germination percentage. PEG6000 with -1.0 MPa was selected as an efficient priming treatment condition at 15~circC , 4 days. This study suggested that priming treatment to sesame seed would be an effective technique enhancing sesame seed germination and shortening time to the T50 at the field condition, but the efficiency of priming treatments to the sesame seed would be strongly dependent on individual or integrated conditions of priming agent, concentration, temperature and duration etc.

Fifty-four sesame oils were assayed for authenticity by measurement of linolenic acid content (which is less than 0.5% by weight in genuine oil). Sesame oils (A-F) from major companies and oils (G-J) extracted from sesame seeds in our laboratory were used as standards for comparison. By fatty acid composition analysis, 33 of 54 samples showed levels of linolenic acid more than 0.5% by weight. In addition, the ratio of linoleic acid to oleic acid (C18:2/C18:1) in samples A-F ranged from 1.05 to 1.12, whereas the 54 collected samples showed a wide range of ratios, from 0.92 to 2.21.

This study was conducted to evaluate the growth characters and yield components of 18 collected sesame cultivars to get basic information on the variation for the sesame breeding using principal component analysis. All characters except days to flowering, days to maturity and 1,000 seed weight showed significantly different. Seed weight per 10 are showed higher coefficient of variance. Capsule bearing stem length and liter weight showed positive correlation with seed yield per 10 are. The principal components analysis grouped the estimated sesame cultivars into four main components which accounted for 83.7% of the total variation at the eigenvalue and its contribution to total variation obtained from principal component analysis. The first principal component (Z1 ) was applicable to increase plant height, capsule bearing stem length and 1,000-seed weight. The second principal component (Z2 ) negatively correlated with days to flowering and maturity by which it was applicable to shorten flowering and maturity date of sesame. At the scatter diagram, Yangbaek, Ansan, M1, M2, M4, M7 and M9 were classified as same group, but M10, Yanghuk, Kanghuk, M5, M6, M12 and M13 were classified as different group. This results would be helpful for sesame breeder to understand genetic relationship of some agronomic characters and select promising cross lines for the development of new sesame variety.

Phytochemical investigation of the sesame dregs of Sesamum indicum was conducted by open column and prep-HPLC chromatography. Two phytosterols (1 and 2) and two lignans (3 and 4) were isolated from the MeOH extracts of sesame dregs, and identified as β-sitosterol (1), daucosterol (2), sesamin (3), and sesamolin (4) by spectral analysis. Although these compounds were already isolated from sesame, it is important that they were still main phytochemical components in the sesame dregs.

To obtain some information on the change of antioxidant components of seeds during grain filling stage as affected by the sowing dates, lignan compounds were investigated according to days of flowering under different sowing dates. Sesamin and sesamolin contents showed significantly different by days of flowering and varieties. Both of sesamin and sesamolin content increased after flowering and reached highest at 40 days of flowering, but they started to decrease thereafter. Sesamin and sesamolin contents of sesame seeds changed with sowing dates. Generally, late sowing date of May 30 showed relatively higher accumulation rate of sesamin and sesamolin contents rather than other sowing dates, but overall patterns were a little different by varieties and lignan compounds. In case of variety Yangbaek, sesamin and sesamolin contents showed relatively higher at sowing date of May 30, but variety Yanghuck showed higher sesamin and sesamolin contents till 20 days of flowering when sowing date of May 30, but it showed to change that both lignan contents were relatively higher under sowing date of May 10.

This study evaluated the effect of organic fertilizer on the yield of sesame and the movement of nitrogen and carbon in soil under mulching cultivation. The seven fertilizer treatments investigated are as follows: 1) 8-4-9 kg N-P2O5-K2O 10a-1-control, 2) 80% of control treatment N from mixed expeller cake (MEC)-MEC 80%, 3) 100% of control treatment N from MEC-MEC 100%, 4) 80% of control treatment N from mixed organic fertilizer (MOF)MOF 80%, 5) 100% of control treatment N from MOF-MOF 100%, 6) 80% of control treatment N from latex coated urea complex fertilizer (LCU)-LCU 80%, and 7) no nitrogen. The soil mineral N available in the applied organic MEC and MOF fertilizers was less than the available N in the applied urea fertilizer during the early growth stage of sesame but the amount was observed to increase with time. The concentration of nitrate-N in leached solution 35 days after application was highest at 75 mg l-1 in the control, followed in descending order by MEC 100%, MOF 100%, LCU 80%, MOF 80% and MEC 80%. The concentration of nitrate-N in the leachate in all treatments was observed to rapidly decrease 45 days after fertilizer application and recorded at less than 20 mg l-1 65 days after application. The concentration of dissolved organic carbon in the leached solution and organic carbon in the soil of the organic fertilizer plots was higher than in the urea- and LCU-fertilized plots during the whole growing period. The yields of sesame applied with MEC and MOF organic fertilizers were 2 to 7% higher than those fertilized with urea. The amount of retained mineral N or organic carbon in the soil is higher in the LCU and organic fertilizer plots than in the urea-fertilized treatments, which resulted in the reduction of nitrate leaching, increasing N efficiency and sesame yield.

Application of genotype by environment (G~;~times~;E ) interaction would be used for identifying optimum test condition of the varietal adaptation in the establishment of breeding purpose. Yield and yield components were used to perform additive main effect and multiplicative interaction (AMMI) analysis. Significant difference for G~;~times~;E interaction were observed for all variable examined. For yield, 0.18 of total sum of squares corresponded to G~;~times~;E interaction. Correlation analysis was carried out between genotypic scores of the first interaction principal component axis (IPCA 1) for agronomic characters. Significant correlations were observed between IPCA 1 for yield and capsule bearing stem length (CBSL), number of capsule per plant (NOC). The biplot of grain yield means for IPCA1 which accounted for 34% of the variation in total treatment sums of squares showed different reaction according to G~;~times~;E interaction, genotypes and environments. Taegu showed relatively lower positive IPCA1 scores, and it also showed smaller coefficient variation of yield mean where it is recommendable as a optimal site for the sesame cultivar adaptation and evaluation trial. In case of variables, Yangbaek and M1 showed relatively lower IPCA1 scores, but the score direction showed opposite each other on the graph. Ansan, Miryang1, Miryang4, and Miryang6 seemed to be similar group in view of yield response against IPCA1 scores. These results will be helpful to select experimental site for sesame in Korea to minimize G~;~times~;E interaction for the selection of promising genotype with higher stability.

참기름, 들기름에서 벤조피렌의 생성경로 및 감소화 방안을 확립하고자 본 연구를 시도하였다. 산지 및 종류에 따라 다소간의 차이는 있었으나 근본적으로 국내산 및 수입산 참깨, 들깨에는 각각 및 의 벤조피렌이 함유되어 있었다. 이 참깨, 들깨를 에서 20분간 볶은 후에는 각각 및 의 벤조피렌이가 검출되었고, 이를 압착한 후 얻어진 기름 중에서는 각각 및 의 벤조피렌이 검출되었다. 이는 볶음-압착공정을 밀폐형으로 수행할 경우에는 외부의 공기를

참깨 연작장해 경감 녹비작물을 선발하기 위하여 9월 하순~10월 중순에 호밀, 헤어리벳치, 귀리 등을 파종 후 이듬해 5월 상순 환원하여 2004년부터 3년간 시험한 결과, 윤작에서 생육 및 수량이 양호하여 단일연작 48.9kg/10a 대비 33% 증수되었으며, 연작 간에는 녹비작물재배 시 양호하여 10~15% 증수되었고, 녹비작물재배 간에는 호밀재배에서 가장 양호한 경향이었다. 윤작 시 연작에 비하여 병발생, 토양의 시들음병균 밀도가 낮은 경향이었으며, 연작 간에는 녹비작물재배 시 양호한 경향이었다. 녹비작물재배 간에는 호밀과 헤어리벳치재배에서 양호하였고, 공극률은 윤작과 연작의 호밀재배에서 가장 높았다. 연작기간이 경과할수록 시들음병 및 역병 발생이 많았으며, 연작 간에는 호밀재배에서 가장 적게 발생되었고, 수량도 가장 많았다.

This study was conducted to evaluate the effect of slow release fertilizers (SRF), crotonylidene diurea (CDU) and latex coated urea (LCU), on nitrogen (N) use efficiency (NUE) and nitrate-N leaching in a silty clay loam soil under polyethylene film mulching (PFM) for sesame cultivation. In PFM plot, concentrations of NO3-N and NH4-N in SRF applied soils were less than that in the urea plot during the whole growing period. However, NO3-N and NH4-N in all the non-mulched plots (NM) were not significantly different. Urea-N in soil treated with SRF was higher than urea plot until 50 days after application and was comparable in all the treatments after 50 days. NO3-N concentrations in leached solution in 21 days after urea fertilization in PFM and NM were 26 mg L-1 and 83 mg L-1 , respectively. However, NO3-N in leached solution at applied CDU and LCU was less than that of urea similar to nitrate concentration in soil. NO3-N in leached solution in applied CDU and LCU in 44 days after application was about 25% lower than that urea plot and PFM, while the NO3-N concentration of CDU and LCU treatment in NM did not changed. Application of SRF increased the yield of sesame and N recovery compared to urea and there was a little difference between SRF and N levels. In conclusion, application of 80% N level with SRF increased N recovery and reduced nitrate leaching without reduction of yields compared with urea application.