Nuclear ribosomal DNA (rDNA) was analyzed to identify inter-specific genetic relationships among 8 Cymbidium species (Cymbidium insigne, C. ensifolium, C. marginatum, C. faberi, C. gyokuchin, C. kanran, C. forrestii, and C. goeringii). Nuclear rDNA including 2 internal transcribed spacer (ITS) regions and 5.8S, was amplified using polymerase chain reaction and sequenced. The sequences were compared via pair-wise multiple alignment to determine the genetic relationships among the studied species. The lengths of the ITS1, ITS2, and 5.8S regions were 235 bp, from 255 bp to 257 bp, and from 153 bp to 165 bp, respectively. Sequence similarities in the ITS region ranged from 78.7% between C. gyokuchin and C. kanran to 96.8% between C. ensifolium and C. kanran. A phylogenetic tree was constructed from nuclear rDNA nucleotide sequence data of the 8 cymbidiums and 1 outgroup species to estimate genetic relationships. The tree revealed that cymbidiums could be classified by their ecological traits, such as their temperature preference or inflorescence pattern. The phylogenetic data is applicable for identification, classification, and breeding of cymbidiums.

We conducted experiments to compare the catch rate of bigeye tuna and yellowfin tuna between circle hooks and straight shank hook in the Korean tuna longline fishery at the eastern and central Pacific Ocean from 2005 to 2007. We analyzed difference of fork length, survival and hooking location between a circle hook and a straight shank hook for both tunas, respectively. There was no difference in the mean fork length size of yellowfin tuna caught on the two type of hook but bigeye tuna was significant. In case of survival, there was no difference between two hook type, but the difference of hooking location was significant for both species. We also analyzed to find determinants of both tunas catch rate using generalized linear models (GLMs) which were used latitude, longitude, year, month, depth, hook type, bait type and so on as independent variables. Spatial factors, latitude and longitude, and temporal factors, year and month, affected catch rate of bigeye tuna and yellowfin tuna. And also, depth such as a marine environment factor was influenced on catch rate.

본 연구에서는 GC-MS를 이용하여 괴산군 지역에서 생산되고 있는 각종 고추에 대해 품종별로 capsaicin 및 dihydrocapsaicin의 함량을 조사하였고, 괴산청결고추조합에서 생산하여 시판되고 있는 고춧가루에 대해서도 매운맛 등급별 capsaicin과 dihydrocapsaicin의 함량을 조사하여, 고춧가루의 매운 정도를 capsaicin의 함량으로 표시하는 방법을 제시하고자 하였다. 고추의 품종별 capsaicin의 함량은 청양이 123.62 mg%, 한반도는 95.97 mg%, 건초왕은 84.00 mg%, 우리건은 70.62 mg%, 금빛은 55.36 mg%, 무한질주은 45.19 mg%, 독야청청은 43.98 mg%, PR대촌은 38.92 mg%, 대박나는 31.63 mg%, 신세계는 29.82 mg%, 장수왕은 28.23 mg%, 오광은 34.42 mg%, 대통은 25.18 mg%였으며, 시판 고춧가루제품의 경우 아주매운맛 등급이 101.98 mg%, 매운맛이 67.63 mg%, 보통맛이 37.74 mg%, 순한맛이 14.73 mg%이었다. 본 실험결과 및 아주매운맛, 매운맛, 보통맛, 순한맛의 4등급으로 분류되어 현재 시판되고 있는 매운맛 등급을 참고하여, capsaicin 함량에 따른 매운맛의 등급별 분류를 7등급으로 제시하였다. 즉 매운맛 1등급은 120 mg%이상, 2등급은 100~120 mg%, 3등급은 80-100 mg%, 4등급은 60-80 mg%, 5등급은 40-60 mg%, 6등급은 20-40 mg%, 7등급은 20 mg%이하로 분류하였다. 이를 바탕으로 고춧가루를 이용해서 만드는 김치, 고추장, 양념소스류 등의 가공식품제조 시 문제되는 매운맛의 비객관적, 불균일성을 개선하며 재현성을 유지할 수 있을 것으로 사료 된다.