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        검색결과 19

        10.
        2019.04 구독 인증기관·개인회원 무료
        Spotted wing drosophila, Drosophila suzukii, causes damage on several fruits include strawberry in Korea. D. suzukii lays egg inside fruit surface, and this behavior makes D. suzukii hard to detect, so many countries designated D. suzukii as a quarantine pest. Recently, Australian government demand methyl bromide fumigation on Korean export strawberry to prevent D. suzukii invasion. In this study, we tested methyl bromide to control D. suzukii, and also tested separate and concurrent treatments of methyl bromide to evaluate phytotoxicity on strawberry. When 40 g/m3 of methyl bromide was treated for 3 hours, all stages of D. suzukii were completely controlled. When treated separate and concurrent with cold temperature treatments of methyl bromide, there’s no specific phytotoxicity on strawberry. This result indicates that separate and concurrent treatments of methyl bromide do not cause phytotoxic effect on strawberry and methyl bromide is effective to control D. suzukii.
        11.
        2019.04 구독 인증기관·개인회원 무료
        Drosophila suzukii Matsumura (Diptera: Drosophilidae) is known as a pest that has a wide host range. This study was carried out to investigate the occurrence of D. suzukii by trapping D. suzukii in Korean strawberry greenhouse and its surrounding woodland from November, 2017 to April, 2019. As a result of trap monitoring, the greatest capture was recorded in fall and secondary peak was confirmed in summer in a lower capture. It was confirmed that there was a statistically significant difference between the occurrence among inside and outside of the greenhouse and the woodland. The strawberry greenhouse cultivation area of Korea is mainly located in the plain region, whereas most of the other host plants for D. suzukii are usually grown at the ridge of the mountain or hillside. Therefore, if considering the living environment of host plant, monitoring result that showed more capture in woodland than strawberry greenhouse would be explained.
        12.
        2018.10 구독 인증기관·개인회원 무료
        Spotted wing drosophila, Drosophila suzukii, is an important pest of grape and strawberry because it lays egg inside fruit by damaging fruit surface. Recently, D. suzukii has been widely spreaded and causes several problems, so many countries include Australia designated D. suzukii as an important quarantine pest. Because of this, Korean farmers have trouble to export strawberry. In this study, we tested ethyl formate and phosphine to control D. suzukii, and also tested concurrent treatment of ethyl formate and phosphine to reduce phytotoxicity and enhance efficacy. When treated 35g/m3 of ethyl formate for 4 hours, mortality of egg, larvae, pupae and adult stages of D. suzukii was 22.2%, 21.1%, 19.2% and 28.3%, respectively. When treated 1g/m3 of phosphine for 24 hours, all stages of D. suzukii was completely controlled, but caused phytotoxic effect on strawberry. When treated with 35g/m3 of ethyl formate and 1g/m3 of phosphine concurrently for 4 hours, efficacy has been increased with less phytotoxicity than separate treatment. This result indicates that concurrent treatment enhanced efficacy with less phytotoxicity.
        13.
        2018.04 구독 인증기관·개인회원 무료
        The effects of cold storage temperature and exposure duration on immature stages of spotted-wing drosophila (SWD) Drosophila suzukii (Diptera: Drosophilidae) on ‘Campbell Early’ grapes were examined to establish a phytosanitary control method. The immature stages (eggs, larvae and pupae) of SWD were all dead after a 6-day cold treatment at 1°C and 8-day cold treatment at 1.5 and 2°C. Small-scale tests using pupae, which were the most-cold tolerant stage, confirmed the validity of the selected temperature and exposure durations. Conversely, the 8- and 10-day at 1°C treatments showed 100% mortality, suggesting that these treatments can guarantee quarantine security against infestations of SWD on exported ‘Campbell Early’ grapes.
        14.
        2017.10 구독 인증기관·개인회원 무료
        The spotted wing drosophila (SWD), Drosophila suzukii, is classified exotic quarantine pest in Australia and EU, soSWD-free must be supported by evidence of surveys and phytosanitary measures in exported host agricultural commoditiesin Korea. From a quarantine control point of view, SWD is importantly considered as model insect pest for exotic fruitflies (Bactrocera dorsalis) in Korea as well because of similarity in ecological cycles. In evaluations of ethyl formate(EF) only and combined cold treatment to kill eggs and larvae of SWD, the combined EF fumigation applied at LCt50%(50% killed lethal concentration X time) and cold treatement (5℃) for > 5 days showed the promise to new conceptfor eradicating quarantine pest and these could be helpful to pre-develop exotic fruit fly management in Korea.
        15.
        2016.10 구독 인증기관·개인회원 무료
        We developed a simple molecular detection tool that rapidly and accurately identifies Spotted wing Drosophila (SWD) without sophisticated instruments or expertise. We first identified a gene that is present in the SWD genome but not in that of any other insect species. Then, we developed the loop-mediated isothermal amplification (LAMP) assay, which was designed based on genomic DNA of SWD specific gene. This LAMP assay detected only genomic DNA from SWD—not from Drosophila melanogaster. In addition, this assay could detect genomic DNA in SWD geographical strains collected from 8 different locations in Asia, Europe, Hawaii, and the USA. Our LAMP assay could be a useful detection tool for identifying SWD rapidly in the field. This work was carried out with the support of the Cooperative Research Program for Agriculture Science & Technology Development (Project No: PJ0116302016).
        16.
        2016.10 구독 인증기관·개인회원 무료
        The spotted-wing drosophila (SWD), Drosophila suzukii (Diptera: Drosophilidae), is an economically damaging pest that feeds on most thin-skinned fruits. In this study, we sequenced portions of the mitochondrial (mt) COI and ND4 genes from a total of 195 individuals collected mainly from Korea. A total of 139 haplotypes were obtained from the concatenated COI and ND4 sequences. A dataset combining GenBank sequences with our own data identified a total of 94 worldwide COI haplotypes with a maximum sequence divergence of 5.433% (32 bp). A rough estimate of genetic diversity in each country showed higher diversity in ancestral distributional ranges, but the invasion over Asian countries seems to have been substantial because haplotype diversity was only 2.35-3.97-fold lower in the USA, Canada, and Italy than that in the populations ancestral ranges.
        17.
        2016.04 구독 인증기관·개인회원 무료
        The spotted-wing drosophila Drosophila suzukii (Diptera: Drosophilidae) is an Asian species introduced into North America and Europe. It damages a wide variety of thin-skinned fruits. We sequenced the complete mitochondrial genome (mitogenome) of D. suzukii to better understand the mitogenomic characteristics of this species and understand phylogentic relationships of Drosophila. The 16,230-bp complete mitogenome of the species consists of a typical set of genes, including 13 protein-coding genes (PCGs), two rRNA genes, and 22 tRNA genes, and one major non-coding A+T-rich region, with an arrangement typical of insects. Twelve PCGs began with the typical ATN codon, whereas the COI began with TCG, which has been designated as the start codon for other Drosophila species. The 1,525-bp A+T-rich region is the second longest in Drosophila species for which the whole mitogenome has been sequenced, after D. melanogaster. Phylogenetic analysis with the 13 PCGs of the Drosophila species using Bayesian Inference and Maximum likelihood methods both placed D. suzukii at the basal lineage of the previously defined Melanogaster group, with a strong support.
        18.
        2015.10 구독 인증기관·개인회원 무료
        The spotted-wing drosophila (SWD), Drosophila suzukii (Diptera: Drosophilidae), originally distributed across a few Asian countries, including South Korea, has invaded North America and Europe, but is absent from Australia. In order to export the South Korean grape cultivar Campbell Early to Australia, its potential to serve as oviposition and development medium for SWD must first be determined. In this study, we determined the oviposition and development potential of SWD on Campbell Early, after elucidating the SWD life cycle and establishing an artificial diet-based mass-culturing system. An investigation of the life cycle under five temperature regimes (16, 19, 22, 25, and 28°C) showed that the durations of the egg, larval, and adult stages were shorten when temperature was increased from 16, 19, 22, 25, and 28°C, but pupal duration was shortest at 25°C and extended again at 28°C. A test of oviposition and development potential of SWD on Campbell Early grape clusters showed oviposition of 30.8 ± 6.8 eggs per cluster of injured grapes and 157.7 ± 16.2 eggs on a culture dish of artificial diet. However, in a similar experiment using uninjured grape clusters, only a single egg was deposited on the grape skin, which soon dried. In light of these results, newly harvested grapes left at vineyards during daily harvests are unlikely to serve as an oviposition and development medium for SWD, as long as the grapes remain uninjured.
        19.
        2014.10 구독 인증기관·개인회원 무료
        The spotted-wing drosophila (SWD), Drosophila suzukii (Diptera: Drosophilidae), was originally observed in a few Asian countries, but is now found even in North America and Europe. Genetic information on geographic variation and relationship may broaden our understanding of origin and migration. As a first step, in this study, a portion of mitochondrial COI gene was sequenced to understand genetic relationship and diversity in Korea. Sequencing of 104 individuals provided 57 haplotypes, with the maximum sequence divergence of 1.5%, suggesting high haplotype diversity and moderate sequence divergence. Comparison to GenBankregistered D. suzukii haplotypes (possibly from Spain, Portugal, USA) has shown 100% sequence identity to most of the haplotypes found in this study, but two USA sequences were found to be independent haplotypes, with the sequence divergence ranging from 0.5% ~ 1.4% from our samples in the 553-bp comparison. Phylogenetically, no separable group was found, but, population genetically, the only Chinese population, Sandong, was significantly differentiated (p < 0.05) from all Korean populations, without sharing any haplotype. Among 28 pairwise comparisons of Korean populations only two comparisons showed a significant genetic differentiation, indicating that no population in Korea is completely isolated. Geographically, one haplotype (SWDBA06) was relatively widespread (five among nine localities) and a few haplotypes were found in more than one locality, but most haplotypes were restricted in a locality as a single individual. Overall, high rate of per generation female migration (Nm = 0.75 ~ infinite) and low level of geographic separation (FST=0~0.40) among localities were characteristic. Current data is limited mainly to Korean localities, thus, an expanded study may provide further scrutinized analysis for the fly.