This study was to investigate the improvement of immune activities of the extracts from Codonopsis lan-ceolata by stepwise steaming process and high pressure process. The phenol contents was 8.742㎍/㎎ which was higher thanthat from conventional extraction using 70% ethyl alcohol at 80℃ for 24hours. All of extracts at a concentration of 1.0㎎/㎖showed relatively low cytotoxicity on human normal kidney cell (HEK293) in range of 16 19%. The immune B and T cellgrowth was improved by extracts using the steamed and high pressure precess of C. lanceolata up to 180×10⁴cells/㎖ and96×10⁴cells/㎖, respectively. The extract prepared also greatly increased the secretion of both IL-6 and TNF-α from thestepwise steamed and high pressure process. This results can conclude that stepwise steamed and high pressure processeffectively released active biomaterials which could important role in enhancing immune activity in the body.
This study was performed to enhance contents of low molecular weight ginsenoside Rh2 and Rg3 using an ultra high pressure and steaming process in wild cultured-Root in wild ginseng. For selective increase in contents of Rg3 and Rh2 in cultured wild ginseng roots, an ultra high extraction was applied at 500MPa for 20 min which was followed by steaming process at 90℃ for 12 hr. It was revealed that contents of ginsenosides, Rb1, Rb2, Rc and Rd, were decreased with the complex process described above, whereas contents of ginsenoside Rh2 and Rg3 were increased up to 4.918 mg/g and 6.115 mg/g, respectively. In addition, concentration of benzo[α]pyrene in extracts of the cultured wild ginseng roots treated by the complex process was 0.64 ppm but it was 0.78 ppm when it was treated with the steaming process. From the results, it was strongly suggested that low molecular weight ginsenosides, Rh2 and Rg3, are converted from Rb1, Rb2, Rc, and Rd which are easily broken down by an ultra high pressure and steaming process. This results indicate that an ultra high pressure and steaming process can selectively increase in contents of Rg3 and Rh2 in cultured wild ginseng roots and this process might enhance the utilization and values of cultured wild ginseng roots.