In this study, we proposed a novel concept of liquid level indicator that can stably measure the liquid level even with changes in liquid density by enormous temperature changes. This uses a distributed optical fiber sensor based on Rayleigh scattering with high spatial resolution. Additionally, taking advantage of the flexibility of an optical fiber sensor, we introduced a bendable supporting structure and made it possible to freely adjust the angle for ease of installation of the liquid level indicator. In the proof-of-concept experiment using a prototype incorporating the proposed concept, we confirmed that the interface location can be effectively detected by utilizing the difference in heat transfer characteristics between liquid and gas phases, demonstrating the feasibility of liquid level measurement. Such a liquid level indicator is expected to enable more accurate level measurements in environments with huge temperature changes and to be conveniently used even in situations where installation and maintenance of the level meter are challenging due to complex internal structures.

양산시 동면 금산리 일원의 공사현장 사면 3개 지점에서 미고결 퇴적층을 절단하는 단층이 확인되었으며, 노두 단면에서 관찰되는 단층의 상세 구조분석을 수행하였다. 이곳 금산리 지점은 기존에 제4기 단층운동이 보고된 가산단층 지점으로부터 북쪽으로 약 0 .6 k m 떨어진 곳에 위치한다. 관찰된 총 6조의 단층들은 14o-32oE 주향을 가지고 3조의 단 층들은 77o-87oNW, 나머지 3조의 단층들은 53o-62oSE로 경사진다. 단층에 의해 절단된 미고결 퇴적층은 동편의 금정산 에서 유래된 선상지 역암으로 주로 화강암 또는 화산암 기원의 직경 0.5m 이상의 거력으로 구성된다. 단층면 상에 발 달하는 단층조선은 역이동성 성분이 포함된 우수향 주향이동단층 운동감각을 지시하며, 이러한 변형특성은 한반도 현생 응력환경인 동북동-서남서 압축응력과 부합한다. 사면에서 관찰되는 기반암과 미고결 퇴적층과의 부정합면을 기준으로 산정한 단층의 겉보기 수직변위는 동편이 15 m, 서편이 1 m이다.

본 연구에서는 식품접객업소에서 시중 판매되는 더치커 피의 미생물 오염도를 조사하여 유형에 따른 차이를 살펴 보고자 하였다. 경기도 내 커피전문점에서 유통되는 더치 커피 70건을 대상으로 위생지표균, 식중독균, 진균류 오염도를 조사하였으며 추출방법에 따른 일반세균과 진균류의 오 염도를 비교하였다. 일반세균수는 0.74-6.21 log CFU/mL 범 위로 검출되었으며 식품 유형에 따른 평균 검출량은 식품 접객업소 조리식품(3.04 log CFU/mL)이 액상커피(2.08 log CFU/mL)보다 높게 나타났다. 액상커피는 검출된 6건 중 3건이 세균수 기준을 초과하여 부적합이었다. 식중독 원 인균은 70건 모두 불검출이었다. 진균수는 0.70-4.00 log CFU/mL 범위로 검출되었으며 식품유형에 따른 차이는 없 었다. 추출방법에 따른 미생물 오염도는 원두 혼합 여부 와 추출 시간에 따라 비교하였으며, 일반세균수와 진균수 모두 유의적인 차이는 없었다(P>0.05). 시중 유통되는 더 치커피는 일반세균과 진균류에 대한 오염도가 높으므로 위생적인 관리와 적절한 온도 유지를 통해 미생물 오염을 줄이는 것이 중요하며 안전성 확보를 위해 지속적인 모니 터링이 필요할 것으로 생각된다.

저탄소 공정을 이용한 추출 기술인 초음파, 마이크로파 및 초고압 추출 공정기술의 이산화탄소 배출량(TCO2)과 얻어진 저분자 진세노사이드 총량의 상관관계를 비교하였다. 기존의 공정인 열수 추출 공정의 TCO2 배출량은 약 0.4 TCO2로 나타났다. 마이크로파 추출 공정의 경우 0.1437 Ton 당 CO2를 배출하는 것으로 확인 되었다. 또한, 초음파 추출 공정의 경우 0.0862 Ton 당 CO2를 배출하는 것을 확인 하였으며, 초고압 추출 공정의 경우 0.1014 Ton당 CO2를 배출하는 것을 확인 하였다. 저탄소 공정별 저분자 진세노사이드의 전환된 양을 측정한 결과 마이크로파 추출 공정의 경우 약 246.65% 정도 증진된 것을 확인 할 수 있었다. 또한, 초음파 공정의 약 275.71% 증진된 결과를 보였다. 초고압 추출 공정의 경우에는 약 295.21% 증진된 결과를 얻었다. 전체적으로 열수 추출 공정의 경우 얻어진 저분자 진세노사이드가 적은 반면 CO2 배출량이 매우 높은 것을 확인하였다. 반대로, 저탄소 추출 공정인 마이크로파, 초음파 및 초고압 공정의 경우 얻어진 저분자 진세노사이드의 양이 높으며, 방출되는 CO2의 양이 기존의 재래 방법보다 적은 것을 확인 하였다. 따라서, 저탄소 추출 공정인 마이크로파, 초음파, 초고압 추출 공정을 통해 인삼을 효과적으로 추출을 할 수 있으며, 친환경 저탄소 공법을 통해 CO2 발생량을 억제하여 경제적으로 천연물을 추출할 수 있을 것으로 사료된다.

[1,2,4]-Triazole derivatives were synthesized by 5 steps. Benzimidazole was refluxed with ethyl chloroacetate to give 1H-benzimidazole-acetic acid ethyl ester (1) over 52% yield. Ester (1) was refluxed with hydrazine hydrate in the presence of ethanol to afford 1H-benzimidazole-1-acetic acid, hydrazide (2). 5-Benzoimidazol-1-ylmethyl-4H-[1,2,4]triazole-3-thiol (4) was made via coupling of compound (2) with methyl isothiocyanate, followed by cyclization of 1H-benzimidazole-1-acetic acid, 2-[(methylamino) thioxomethyl]hydrazide (3) on reflux, and finally the target compounds (6a-6v) were synthesized by general substitution reaction. Compounds (6a-6v) were screened for T-type calcium channel blocker using the fluorescence assay by FDSS6000. All compounds (6a-6v) did not show better activities than control compound, mibefradil.

한반도 동남부에서 가장 규모가 큰 마이오세 포항분지의 남서부를 대상으로 상세한 지질도 작성과 지질구조를 분석하여 분지 발달사를 해석하였다. 포항분지 남서부는 북북동 방향의 분지 내 지구내지루에 의해 기하와 변형사가 서로 다른 서쪽의 보문구역과 동쪽의 오천구역으로 나누어진다. 보문구역은 포항분지 초기 확장 이후 거의 변형되지 않은 반면, 오천구역은 충전물의 퇴적 이후에도 후기 변형이 지속·중첩된 지역이다. 따 라서 보문구역은 포항분지 최초 확장 형식에 대한 중요한 정보를 제공한다. 보문구역은 북북동 방향의 정단 층들과 북북서(북서) 방향의 우수향 주향이동단층 분절들이 지그재그로 이어진 서편 경계단층에 의해 침강이 주도되었으며, 이 경계단층은 남쪽으로 한반도 동남부 마이오세 지각변형의 서쪽 한계선이며 우수향 주향이 동성 주변위대로 알려진 연일구조선과 연결된다. 따라서 포항분지는 17-16.5Ma 경 활동을 시작한 연일구조 선 북쪽 말단에 우향스텝 형태로 생성된 북북동 방향의 정단층 또는 사교이동단층에 의해 서북서-동남동 방 향으로 확장된 횡인장단층말단분지로 최초 침강된 것으로 해석된다. 이로 인해 보문구역에는 서쪽으로 갈수록 분 지의 깊이가 더 깊어지는 북북동 방향의 비대칭 지구 또는 반지구가 생성되었다. 이후 포항분지의 확장과 변 형은 오천구역을 포함하는 보다 동편 지역으로 이동하였다.

Cosmetic activities of the leaves of Curcuma longa L. were compared according to different drying methods, to expand the use of the by-products of Curcuma longa L. The highest extraction yield of 29.4% was obtained from vacuum drying process (VD), whose value was very close to 29.2% from freeze drying process (FD). Relatively lower extraction yield were observed as 24.5% and 25.3% from the raw leaf (RL) as control and hot air drying process (HD). The cytotoxicity was observed lower FD and VD than RL and HD. It shows that cytotoxicity was closely related to cosmetic activities such as tyrosinase and melanin inhibition activities by showing much better cosmetic activities of the extracts from both FD and VD than those from the RL and HD. It was interesting that was differences of the cosmetic activities and cytotoxicity between FD and VD, which implies that VD method should be a more efficient process than FD method in terms of drying time and operation costs: 6 hours vs 24 hours and 3-5 times higher extraction costs in drying. It was observed that VD is more excellent dry method than others. This result could be utilized to effectively dry other soft plant biomass.

This study was performed to enhance contents of low molecular ginsenoside using steaming and fermentationprocess in low quality fresh ginseng. For increase in contents of Rg2, Rg3, Rh2 and CK in low quality fresh ginseng, a steam-ing process was applied at 90℃ for 12hr which was followed by fermentation process at Lactobacillus rhamnosus HK-9incubated at 36℃ for 72h. The contents of ginsenoside Rg1, Rb1, Rc, Re and Rd were decreased with the steaming associ-ated with fermentation process but ginsenoside Rg2, Rg3, Rh2 and CK increased after process. It was found that under thesteaming associated with fermentation process, low molecule ginsenosides such as Rg2, Rg3, Rh2 and CK were increased as3.231㎎/g, 2.585㎎/g and 1.955m/g and 2.478㎎/g, respectively. In addition, concentration of benzo[α]pyrene in extracts ofthe low quality fresh ginseng treated by the complex process was 0.11ppm but it was 0.22ppm when it was treated with thesteaming process. This result could be caused by that the most efficiently breakdown of 1,2-glucoside and 1,4-glucoside link-age to backbone of ginsenosides by steaming associated with fermentation process. This results indicate that steaming pro-cess and fermenration process can increase in contents of Rg2, Rg3, Rh2 and CK in low quality fresh ginseng.

According to previous reports, antioxidant activities of Codonopsis lanceolata could be increased by a steaming process. This study was performed to improve its antioxidant activity and skin whitening activities of C. lanceolata by high pressure and stepwise steaming complex process. The complex processed C. lanceolata showed highest free radical scavenging acitivity as 45.21%, and for phenol and flavonoid contents, complex processed C. lanceolata contained higher than those from conventional extraction process or steaming process alone. The Cytotoxicity of all C. lanceolata extracts also showed low cytotoxicity against human fibroblast cell (CCD-986sk) as 4.49 ~ 10.40%. In whitening activity, high inhibition of tyrosinase activity was estimated as 25.08% by adding the extracts from complex process. We found that whitening and antioxidant activity of complex processed C. lancolata extract was higher than those obtained from conventional extraction and a steaming process because various kinds of antioxidant compounds could be easily released by combined process, compared to one of each process.

This study was performed to enhance contents of low molecular weight ginsenoside Rh2 and Rg3 using an ultra high pressure and steaming process in wild cultured-Root in wild ginseng. For selective increase in contents of Rg3 and Rh2 in cultured wild ginseng roots, an ultra high extraction was applied at 500MPa for 20 min which was followed by steaming process at 90℃ for 12 hr. It was revealed that contents of ginsenosides, Rb1, Rb2, Rc and Rd, were decreased with the complex process described above, whereas contents of ginsenoside Rh2 and Rg3 were increased up to 4.918 mg/g and 6.115 mg/g, respectively. In addition, concentration of benzo[α]pyrene in extracts of the cultured wild ginseng roots treated by the complex process was 0.64 ppm but it was 0.78 ppm when it was treated with the steaming process. From the results, it was strongly suggested that low molecular weight ginsenosides, Rh2 and Rg3, are converted from Rb1, Rb2, Rc, and Rd which are easily broken down by an ultra high pressure and steaming process. This results indicate that an ultra high pressure and steaming process can selectively increase in contents of Rg3 and Rh2 in cultured wild ginseng roots and this process might enhance the utilization and values of cultured wild ginseng roots.

This study compared the contents of low molecular ginsenoside according to fermentation process in low grade fresh ginseng. Low grade fresh ginseng was directly inoculated with a 24 h seed culture of Bifidobacterium Longum B6., Lactobacillus casei., and incubated at 36℃ for 72 h. Bifidobacterium Longum B6 was specifically was found to show the best growth on 3,255×106 CFU/ml after 48 h of fermentation. The content of ginsenoside Rb1, Re and Rd were decreased with the fermentation but ginsenoside Rh2 and Rg2 increased after fermentation process. In the case of low molecular ginsenoside conversion yields were 56.07% of Rh2, 12.03% of Rg3 and 77.11% of Rg2, respectively. In addition, compound-K was irregular conversion yield as long as 72 h of fermentation. This results indicate that fermentation process could increase the low molecular ginsenoside in low grade fresh ginseng.

In this study, we investigated the cosmetic application of Acer mono sap through an ultra-high pressure process. Exposing Acer mono sap to a ultra-high pressure process resulted in 90.1% cell viability of human normal fibroblast cells (CCD-986sk) when added at the highest concentration. Acer mono sap also showed the hightest free radical scavenging activity after the ultra high pressure process. The melanogenesis inhibition rate in cloned M-3 cells was 59.0%. Tyrosinase was inhibited at a rate of 87.2% by adding 100% HPAMS. Anti-wrinkle activity was 78.1%. Acer mono sap showed enhanced storage following the ultra high pressure process. These results indicate that Acer mono sap may be a source for functional cosmetic agents capable of improving antioxidant, whitening, and antiwrinkling effects.

In this study, we investigated antioxidant activities and whitening effects of Acer mono sap by encapsulation of nanoparticles. Acer mono sap was through ultra high pressure process and then encapsulated by lecithin. Nano-encapsulated The nanoparticles of Acer mono sap showed highest free radical scavengering effect as 89.7% in adding sample (1.0 mg/ml), compared to sap of non-encapsulation. It was showed strong inhibition effect on melanin production test by Clone M-3 cells as 47.8%. High inhibitory of tyrosinase was also measured as 85.8% by adding lecithin nano-particle of 1.0 mg/ml. The nano-particles also showed 14.8% of low cytotoxicity against human normal fibroblast cells in adding 1.0 mg/ml of the highest concentration. These results indicate that Acer mono sap may be a source of cosmetic agents capable of improving whitening effect and antioxidant activites.