본 연구는 코칭 전공 대학원생들이 코칭을 전공으로 선택한 이유를 대학원생 들의 관점에서 살펴보고 유형화하여 이들이 실질적으로 코칭 전공 대학원에서 갈망하는 요구를 이해하기 위한 목적으로 진행되었다. 이를 위하여 인간의 주관 적 인식을 분석하기에 적합한 Q방법론을 통해 연구 문제를 분석하였다. 본 연구 에서는 국내 코칭 관련 학과 대학원 석·박사과정 재학 또는 졸업한 사람들 중 목적표집 방법으로 선발된 총 21명의 P표본을 대상으로 40개의 진술문을 구성 하여 연구가 진행되었다. 연구 결과 총 4개의 유형으로 분석되었고 유형Ⅰ은 ‘경 제적 수입보다 사람을 중요시하는 기여형’, 유형Ⅱ는 ‘네트워킹보다 스스로를 중 요시하는 자아 계발형’, 유형Ⅲ은 ‘스펙을 통해 코칭의 학문 발전을 중요시하는 준비형’, 유형Ⅳ은 ‘코칭 철학을 믿는 긍정적 영향력 발휘형’으로 나타났다. 본 연구 결과는 유형화된 대학원생의 코칭전공 선택에 관한 주관성 특성을 바탕으 로 이후 국내 코칭의 학문적 발전을 위해 코칭 관련 학과 대학원의 체계적인 교 육과정 방향성의 틀로 활용될 수 있으며, 나아가 국내 코칭 정착과 발전에 필요 한 기반이 될 수 있을 것이라 기대한다.

Insect cuticle is an extracellular matrix formed primarily from two different biopolymers, chitin and protein. During each molt cycle, a new cuticle is deposited simultaneously with degradation of the old cuticle by molting fluid cuticle degrading-enzymes, including epidermal chitinases (CHTs). Insect CHTs, belonging to family 18 glycosylhydrolase (GH18), have been classified into at least eleven subgroups based on phylogenetic analyses, and group I (CHT5) and group II (CHT10) epidermal CHTs present in molting fluid. In this study we report the physiological function of MaCHT5 and MaCHT10 in the Japanese pine sawyer, Monochamus alternatus. RNAi for either MaCHT5 or MaCHT10 resulted in larval-pupal and pupal-adult molting defects, in which the insects were unable to shed completely their old cuticle and died entrapped in their exuviae. Furthermore, TEM analysis revealed a failure of degradation of the old cuticle in both MaCHT5- and MaCHT10-deficient pharate adults. In the old pupal cuticle, the chitinous horizontal laminar and vertical pore canal essentially remained intact in the endocuticular layer. These results indicate that both CHTs are required for turnover of the chitinous old cuticle, which is critical for completion of insect molting. We also discuss the possible function of two spliced variants of MaCHT10, MaCHT10a and MaCHT10b.

Insect eggshell and cuticle/exoskeleton play vital roles in protecting them from natural environmental stresses. However, these chitinous cuticular extracellular matrices must be degraded at least in part during embryo hatching and molting/ecdysis periods to accommodate continuous growth all the way to the adult stage. In this study, we investigated the functional importance of groups I and II chitinases, TcCHT5 and TcCHT10, in the turnover of chitinous cuticle during both embryonic and post-embryonic development in Tribolium castaneum. RNAi and TEM analyses revealed that TcCHT10 is required for digestion of chitin in the serosal cuticle for embryo hatching as well as in the old cuticle during post-embryonic molts including larval-pupal and pupal-adult metamorphosis. TcCHT10 appears to be able to substitute for TcCHT5 in all these vital physiological events except for the pupal-adult molting in which TcCHT5 is indispensable for complete digestion of chitin in the old pupal cuticle.

The Blastobasidae is microlepidopteran, narrow-winged moth and account of more 500 species among 23genera worldwide. This family is defined by the following characteristics: i) presence of antennal pecten in head; ii) Rs and M1 separate in the hindwing; iii) valva with well-developed digitate saccular processus in male genitalia; iv) aedeagus with a band-like internal sclerite.

The development of biochemical pesticides to replace existing chemical pesticides and the search for new substances with potential insecticidal ability are continuously required. The purpose of this study is to control myzus persicae with pyrethrum biochemical pesticide containing of high concentration of pyrethrin as an active ingredient. Pyrethrum dried powder was extracted with 95% ethanol for different periods of time and it was confirmed that the pyrethrin content increased as the extraction period increased and the extraction conditions for 20 days had the highest yield with 0.2% pyrethrin. We produced a prototype containing 0.13% pyrethrin content (pyrethrin II: 0.06%, pyrethrin I: 0.06%). and in 200 fold dilute spray condition. It showed a 77.2% corrected mortality against myzus persicae which is comparable to chemical pesticide. We are continuously researching to reduce the decomposition of active ingredients and look for additives to increase the insecticidal activity.

Reticulitermes speratus (Kolbe) is economically important pest in East Asia including Korea, Japan and China where they infest wooden structures in urban areas. In this study, we report the first record of R. speratus speratus from Korea, which was verified using soldier morphology and molecular characteristics obtained from a mitochondrial gene. R. speratus speratus Kolbe, 1885 (Blattodea: Rhinotermitidae) are found in several provinces, mainly southern regions in Korea, whereas R. speratus kyushuensis are distributed throughout the country. Our morphological comparison showed that R. speratus speratus can be distinguishable from R. speratus kyushuensis by the ratio of the posterior postmentum width to length. In the molecular comparison, R. speratus speratus revealed genetic differences of 3.06% (range 2.60–4.10%) from R. speratus kyushuensis using cytochrome oxidase subunit II gene sequences.

The salivary glands of hard ticks consist of three types (type I, II, and III) of acini according to their functions and location. The type II and III acini play critical roles in tick salivation, which is likely controlled by a variety of neuropeptides or neurotransmitter via interaction with their receptor, G protein-coupled receptors (GPCRs). Orchestration of dopamine receptor (D1) and invertebrate specific D1-like dopamine receptor (InvD1L) located in type II and III acini precisely control tick salivary secretion via collection of primary saliva in the lumen and expulsion of collected saliva, respectively. The two dopamine receptors (D1 and InvD1L) in Haemaphysalis longicornis were identified as 1278 bp (426 aa) and 1362 bp (454 aa) in length, respectively. Both dopamine receptors were functionally analyzed through Ca2+ and cAMP assay using the heterologous expression system. The transcripts of D1 and InvD1L were profiled from synganglion and salivary glands of female ticks (unfed, 3/18/60/96 post blood meal and replete). D1 and InvD1L were significantly upregulated in the early phase of blood feeding from female H. longicornis. Salivary secretion induced by dopamine was significantly reduced by RNAi of D1 and InvD1L. Interestingly, RNAi of two dopamine receptors induced a significantly longer period of blood feeding in female ticks, which were significantly lighter after feeding than control. Taken together, it was suggested that D1 and InvD1L play critical roles in early and late phase of tick blood feeding for feeding capability.

Due to the concerns over their environmental and health impacts, there have been attempts for shift towards biorational pesticides from synthetic pesticides. Among them, plant essential oils have emerged as promising active ingredients. Due to the complex interactions among their constituents, the bioactivities of essential oils can vary depending on the compositions, which often undermine their stability in efficacy. Here, we present a model-based optimization approach to develop reliable rosemary oil-based biorational pesticide, against two-spotted spider mites, Tetranychus urticae Koch. The ecotoxicity against Daphnia magna and foliar phytotoxicity against Phaseolus vulgaris were also evaluated. Our quadratic models accurately predicted miticidal activity, ecotoxicity, and phytotoxicity. We aimed to maximize, minimize, and minimize these parameters, respectively. We employed seven multi-objective evolutionary algorithms in Matlab. Among them, the nondominated sorting genetic algorithm II with adaptive rotation based simulated binary crossover (NSGA-II-ARSBX) performed best. We experimentally determined the thresholds for miticidal activity and phytotoxicity, based on the current approval process for agricultural pesticide products in Korea. After applying the thresholds, we validated the obtained viable solutions. Our study offers a novel framework to enhance the reliable and responsible use of essential oils as biorational pesticides.

Silicon (Si) has the potential to improve plant growth and stress tolerance. The study aimed to explore Si-involving plant responses and molecular characterization of different Si-responsive genes in alfalfa. In this study, the exogenous supplementation of Si enhanced plant growth, and biomass yield. Si-acquisition in alfalfa root and shoot was higher in Si-supplemented compared to silicon deficient (-Si) plants, implying Si-acquisition has beneficial on alfalfa plants. As a consequence, the quantum efficiency of photosystem II (Fv/Fm) was significantly increased in silicon-sufficient (+Si) plants. The quantitative gene expression analysis exhibited a significant upregulation of the Lsi1, Lsi2, Lsi3, NIP5;1, and NIP6;1 genes in alfalfa roots, while BOR1, BOR4, NIP2, and NIP3 showed no significant variation in their expression. The MEME results further noticed the association of four motifs related to the major intrinsic protein (MIP). The interaction analysis revealed that NIP5;1 and Lsi1 showed a shared gene network with NIP2, BOR1, and BOR4, and Lsi2, Lsi3 and NIP3-1, respectively. These results suggest that members of the major intrinsic proteins (MIPs) family especially Lsi1, Lsi2, Lsi3, NIP5;1, and NIP6;1 genes helped to pass water and other neutral solutes through the cell membrane and those played significant roles in Si uptake and transport in plants. Together, these insights might be useful for alfalfa breeding and genome editing approaches for alfalfa improvement.

The disposal of organic pollutants is one of the important research topics. Some of the studies in this field are based on the degradation of organic pollutants with a catalytic agent. The cobalt tetraoxide/peroxymonosulfate system is an important catalytic system used for the radical degradation of organic pollutants. To increase the catalytic efficiency of such reactions, graphitization of activated carbon used as a support solid and nitrogen doping to the carbon structure are commonly used methods. In this study, cobalt tetraoxide production, N-doping and graphitization were carried out in a single step by heat treatment of activated carbon doped with the phthlocyanine cobalt (II) complex. The catalytic performance of the catalyst/ peroxymonosulfate system was investigated by changing the pH, catalyst, and PMS concentration parameters on rhodamine B and 1,3,5 trichlorophenol, which were used as models. It was seen that the catalysts had 97% activity on rhodamine B in 16 min and 100% on 1,3,5 trichlorophenol in 6 min. It was observed that the catalysts continued to show high catalytic activity for five cycles in reusability studies and had a very low cobalt leaching rate. These results are in good agreement with previously published studies. In line with these results, the synthesized N-doped graphitic carbon/Co3O4 catalyst can be used as an effective catalyst for wastewater treatments.

본 연구는 전라북도 남원시, 장수군과 경상남도 함양군의 경계에 위치한 백두대간 봉화산의 관속식물상을 파악하여, 생물다 양성 보존 및 외래식물의 확산 방지를 위한 기초자료로 활용하기 위하여 조사를 실시하였다. 국립수목원 산림생물표본관에 소장된 봉화산 표본 검토와 함께, 2020년 4월부터 2023년 7월까지 총 15회에 걸쳐 현지조사를 수행하였다. 그 결과, 본 조사 지역의 관속식물은 97과 279속 409종 16아종 32변종 9품종의 총 466분류군으로 조사되었다. 이 중 특산식물은 8분류군, 적색목록 중 취약(VU)에 속하는 종은 1분류군으로 확인되었다. 식물구계학적 특정식물종은 총 57분류군으로 Ⅴ등급에 1분류 군, Ⅲ등급에 9분류군, Ⅱ등급에 14분류군, Ⅰ등급에 33분류군이 각각 발견되었으며, 외래식물은 24분류군, 그리고 생태계교 란야생종은 1분류군이었다. 분류군의 유용성 조사에서 식용 353분류군, 섬유용 3분류군, 약용 71분류군, 관상용 21분류군, 목초용 108분류군, 공업용 2분류군, 잡용 7분류군, 용재용 11분류군으로 나타났다.

남해 금산을 방위, 지형, 해발고, 접근성 등을 고려하여 7구간으로 나누어 식물상을 조사한 결과 관속식물은 117과 373속 602종 5아종 72변종 14품종 693분류이 출현하였다. 이 중에서 양치식물은 11과 24속 45종 1변종의 46분류군, 나자식물은 4과 6속 12종 3변종 15분류군, 피자식물은 102과 343속 545종 5아종 68변종 14품종으로 632분류군이 출현하였다. 산림청에서 지정한 희귀식물은 18과 18속 20종 1변종 21분류군이 출현하였 다. CR등급은 물부추, 자주땅귀개, 칠보치마, 석곡 등의 6분류군, EN등급은 대흥란, VU등급은 끈끈이주걱, 애기등, 땅귀개 등의 4분류군, LC등급은 고란초, 개족도리풀, 히어리 등의 8분류군, DD등급은 단풍박쥐나무와 토현삼 등이 출현하였다. 한국특산식물은 14과 17속 17종 1변종 18분류군이 출현하였다. 식물구계학적 특정식물 58과 91속 102종 1아종 4변종 107분류군으로, Ⅴ등급은 물부추, 애기등, 칠보치마 등의 11분류군, Ⅳ등급은 끈끈이주걱, 히어리, 긴오이풀 등의 11분류군, Ⅲ등급은 바위손, 큰지네고사리, 토현삼 등의 23분류군, Ⅱ등급은 속새, 고란초, 다정큼나무 등의 13분류군, Ⅰ등급은 실고사리, 발풀고사리, 나래회나무 등의 49분류군으로 출현하였다. 외래식물은 28과 55속 64종 1아종 65분류이 출현하였고, 도시화 지수는 11.2%, 귀화율은 6.1%로 나타났다. 이는 우리나라 평균 귀화율 10.3%보다 낮게 출현하였다.