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pp.25-31
This study was performed to determine the purification yield, color value, and total anthocyanin content of purifiedanthocyanins from Bokbunja byproducts for analyzing the optimal conditions of purification processing by responsesurface methodology (RSM) based on HCl or trifluoroacetic acid (TFA) concentration as an acidulent and ethanolconcentration as an eluent. From the results of purification yield, color value, and total anthocyanin content of thepurified anthocyanins, it was shown that HCl was better than TFA for purification processing from Bokbunjabyproducts and the optimal concentration range of ethanol was 60-75%. The optimal purification conditions for HClor TFA, ethanol concentration, and overall desirability by analysis of multiple response surface methodology withthe same weighed value for each dependent variable were 0.84%, 73.12% and 0.77 for HCl acidulent and 1.00%,60.75%, and 0.96 for TFA acidulent, respectively.
