복분자를 개별인정형 건강기능식품 기능성 원료로 개발하기 위하여 지표성분 표준화를 위한 ellagic acid의 분석법 설정과 분석법에 대한 검증(validation)을 실시하였다.그 결과 ellagic acid의 검량선은 R2=0.9999으로 좋은 선형성을 보였으며, 검출한계 0.6µg/mL, 정량한계는 1.9µg/mL였다. 회수율을 측정한 결과에서는 89.0-100.0%로 나왔고, 상대표준편차(RSD)는 0.05-0.14%를 보였으며, 일내와일간 분석에서 RSD는 각각 0.28-1.96%와 0.69-2.49%로나왔다. 따라서 본 연구를 통해 복분자 추출물의 지표물질인 ellagic acid의 분석법은 적합한 시험법임이 검증되었다.

Validation of ellagic acid as a marker compound in the standardization of black raspberry extract developed forfunctional health food, was attempted by an analytical method. Ellagic acid was validated for its LOD (limit ofdetection), LOQ (limit of quantitation), precision, accuracy, and recovery by HPLC relative to the black raspberryextract. It showed a high linearity in the calibration curve with a coefficient of correlation (R2) of 0.9999. The LODand LOQ were 0.6µg/mL and 1.9µg/mL, respectively. The results of the recovery test were 89%-100%. The intra-day and inter-day precision in the ellagic acid for black raspberry extract was 0.28-1.96% and 0.69-2.49%, respec-tively. Therefore, the application of ellagic acid was validated in analytical method as a marker compound in blackraspberry extract.

Abstract
 서 론
 재료 및 방법
  복분자 추출물 제조
  표준용액의 조제
  시험용액의 조제
  HPLC 분석
  분석법의 유효성 검증(Method validation)
 결과 및 고찰
  특이성
  직선성 및 검출·정량한계
  회수율
  정밀성
 요 약
 감사의 글
 References

• 채규서((재)고창복분자연구소) | Kyu Seo Chae
• 손락호((재)고창복분자연구소) | Rak Ho Son
• 박송이((재)고창복분자연구소) | Song Yie Park
• 김기안((재)고창복분자연구소) | Ki-An Kim
• 이태범((재)고창복분자연구소) | Tae-Bum Lee
• 권지웅((재)고창복분자연구소) | Ji-Wung Kwon Corresponding author