In this study, the humidity control effect of a counter-flow ventilator was analyzed in a greenhouse with high relative humidity at night in the winter season. A case of the counter-flow ventilator was 0.96 × 0.65× 0.82(W× D × H, m) and there were heat transfer element and two fans for air supply and exhaust in the counter-flow ventilator. Two counter-flow ventilators were used in this study and the setting humidity of the ventilators was 80%. The temperature and relative humidity at night(18:00-8:00) in the greenhouse were measured. In a greenhouse without a counter-flow ventilator, the average temperature and humidity was 14.9°C, 82.8%, respectively. When the counter-flow ventilator was operated, the corresponding averages were 15.1°C, 79.9%. The independent sample t test of monthly temperature and relative humidity showed no difference in temperature, and a significant difference in relative humidity with 1% of the significance level. Therefore, using the counter-flow ventilator helps to control relative humidity in greenhouse and increase yield.. And further research considering the pros and cons of using the counter-flow ventilator is needed.