The characteristics of rice starch by sampling six rice cultivars for making rice bread were examined. Six rice varieties exhibited different level of amylose content and the ratio of chain length distribution within the amylopectin cluster. Especially, examination by colorimetric measurement of starch-I2 complex showed the maximum absorption wavelength was the highest in Goamibyeo. The X-ray diffraction patterns of starch granules showed the traditional "A" type, except in Goami 2 and Goami 3 and there was difference in crystalline of rice starch. There were significant difference in the changes of swelling power of rice starches through temperature. We could also found that the six rice cultivars had different levels of the hydrolysis rate by 5% glucoamylase. These results could suggest that properties of rice starch impact on the level of loaf formation and specific gravity of rice bread after baking.
Rumex속 식물의 경쟁적 우세원인을 규명하고 친환경 제초제 등의 자원화 연구에 기초자료로 제공하고자 이 식물의 생육특성과 Allelopathy효과를 알아보았다. 돌소리쟁이 수용성추출액을 이용하여 벤트그라스 (Agrostis stolonifera subsp. stlolnifera), 배추(Brassica campestris subsp. napus var. pekinensis), 이탈리안라이그라 스, 토끼풀(Trifolium repens), 가시비름(Amarathus spinosus), 들묵새(Festuca myuros)등의 상대 발아율을 조사하 여 수용성 추출액이 농도가 높아질수록 발아가 억제되는 경향을 볼 수 있었다. 특히 제주도 위해식물인 가시 비름이 수용성추출액 50% 이상에서는 발아가 되지 않는 경향을 보여 선택적으로 가시비름에 많은 억제력을 보이는 것으로 연구되었다. 애기수영의 수용성 추출액을 이용하여 벤트그라스(Agrostis stolonifera subsp. stlolnifera), 도깨비바늘(Bidens bipinnata), 배추(Brassica campestris subsp. napus var. pekinensis), 크림슨클로버 (Trifolium incarnatum), 이탈리안라이그라스, 토끼풀(Trifolium repens), 등의 발아율을 조사하였는데 수용성 추 출액의 농도가 높아질수록 발아가 억제되는 경향을 보였으며, 배추의 경우 100% 수용성 추출액의 농도에서는 발아가 되지 않아 배추의 생장에 억제력이 높게 나타났다. 반면 평균발아일수는 증가하는 것으로 나타났다. Rumex속의 식물의 수용성추출액을 실험하므로써 식물의 선택적 생장억제력을 알수있었으며 이 실험자료를 토대로 친환경농가에서 대상 식물의 방제에 직접 사용해 볼 수 있고, 추후 친환경자재 개발의 기초자료 될 것으로 기대된다.
One objective of soybean breeders is to develop cultivars with elevated oleic acid content. Testing soybean seeds for oleic acid content is possible using gas chromatography (GC), however it is time-consuming and requires destructing the seeds. Using single seeds, we developed a near-infrared reflectance spectroscopy (NIR) calibration equation relating oleic acid measured with GC to oleic acid predicted by NIR. The slope of the regression line of oleic acid measured with GC on oleic acid predicted by NIR and the intercept was not different from zero. A set of 300 soybean seeds was used to calculate the NIR equation and an independent set of 100 soybean seeds was used for validation. This NIRS equation showed significant correlation between reference values and NIRS estimated values based on the SEP, r2, and the RSP of reference data to SEP. This research shows that NIR prediction of oleic acid using intact soybean seeds is accurate and rapid and should be especially useful for early generation screening.
In order to breed the useful mutants in black raspberry, cultivated in Gochang-gun, leaf-petiol explants from shoot-proliferating culture was regenerated after irradiated with 30, 50 and 70 Gy gamma-ray. The LD50 dose of leaf-petiol explants was identified as 30Gy after two months. Morphological characteristics of the variants were observed such as stem variation, existence and nonexistence of spine, spine color, anthocyanin content, width of leaf, thick of leaf compared with donor cultivar. Of the 701 mutants, 14 lines with the useful mutant characters were selected and random amplified polymorphic DNA(RAPD) was carried out to confirm mutation state at the molecular level with 20 primers. There are many bands be revealed only in mutants and disappear in mutants reversely. RAPD analysis revealed a high level of genetic diversity among Control and mutants. Compared to Control, No. 14 mutant showed the highest genetic diversity. Also, the co-dominant SSR markers have allowed the evaluation of heterozygosity. Valuable mutants obtained will be useful for developing new cultivars and studying gene function in molecular level.