Background : Black Non-Woven Fabric Mulch Culture was knowned increased crop Yield and saved weeding labor in Liriope Pilatyphylla Wang et Tang. But to the removal and planting labor is more needed, So some famers are avoidance that culting method. Methods and Results : So this study was experimented in order to selecting optimun removal time in Liriope Pilatyphylla Wang et Tang mulch culture. Removal time were conventional practices (in April next yesr), September, October and November. In early, Plant length, Root length, Leaf number and number of plants was the long and many by the sooner removal time and also, dry weight was heavier. Black non-woven fabric removal labor was saved by the sooner removal time. The main event of weeds were Cyperus serotinus Rottb and Portulaca oleracea L. In harvest time, Plant length and plants of numbers was the longest and heavier at conventional practice (in April next year) and November removed. Tuberous root number was the more in September removal, Because, the tuber was tall and long. Total1y consideration of the including weeds shooting, weeding labor and Growth and development situation, Black non-woven fabric removal optimum time was September or Conventional practices (in April next year). Conclusion : Black non-woven fabric optimum removal time was the september . In this experiment, increased yield 9, income 16 percent than conventional practices (in April next year).

Background : In the year 2015, the area under rhizome of Rehmannia glutinosa in Chungnam province increased to 65% (105 ha) of domestic total cutivation area and is recognized as income crop. Since proper strorage method of rhizome has not been established, rhizome decay rate is so high during storage. This experiment was carried out to establish proper strorage method for stable production of rhizome of Rehmannia glutinosa Methods and Results : Korea jiwhang was used for this experiment and rhizome was cured under vinyl house and room temperature for 1, 2, 3 days respectively. Rhizome was stored for 140 days at –2 - 0℃ after curing, and rhizome quality and saprogen were investigated after storage. Trimming loss was 11.3 - 21.9%, higher in longer curig time and rice hull packing. Moisture content was 70.0 - 75.1%, higher in shorter curing time and no rice hull packing. Curing efficacy was better in vinyl house than room temperature, decay rate by weight and number of rhizome was lowest in 48hrs curing and no hull packing as 0.5% and 2.0% respectively. Identified saprogen during storage was Fusarium oxysporum, Fusarium tricinctum, Penicillium verrucosum, Mucor racemosus. Conclusion : Curing efficacy was better in 48hrs treatment under vinyl house with lowest decay rate and rice hull packing increased decay rate considerd due to saprogen existing in rice hull. 4 saprogens including Fusarium oxysporum were identified during storage of rhizome.

Background : Bai Zhu (Atractylodes macrocephala Koidz.) has been generally harvested in 1-2 years after seedstock planting. Recently, seed is used increasingly for planting in spring and rhizome is harvested in 1 year. In 1 year open field culture, seed harvesting rate is low below 30% and supply and demand of seed is so hard. This study was carried out to improve seed harvesting for stable seed supply and demand in Atractylodes macrocephala Methods and Results : Seed of Atractylodes macrocephala Koidzumi was directly sown in open field, under tunnel and rain shielding condition in mid-April. Tunnel was installed from prebloom, mid-september to seed harvesting time, November using 240 ㎝ wires for 120 ㎝ by interval and 20 ㎝ by height in furrow to assure insect pollination and wind fertilization. In rain shielding condition, plant height was highest as 38.2 ㎝ and number of branches, number of nodes, and content of chlorophyll were higher as 7.8 ea/plant, 8.7 ea/plant, and 65.4 respectively compared to open field and under tunnel. Flowering time was earliest as 23rd September in rain shielding condition and in open field and under tunnel was 26th September. There is no blossom rot in rain shielding condition while blossom rot occurrence was highest in open field by 5 in degree. Seed setting rate was highest as 42.0% in tunnel cultivation and seed weight per plant and 1,000 seed weight were highest as 3.7 g/plant and 22.3 g respectively. Total seed yield was higher as 58% and 65% in tunnel and rain shield condition respectively compared to 29 ㎏/10 a of open field. Tunnel cultivation for seed production of Atractylodes macrocephala is more beneficial for lower installation cost. Conclusion : Tunnel cultivation for seed production of Atractylodes macrocephala is more beneficial due to low blossom rot degree by 1, higher 1,000 seed weight and seed setting rate as 19.0 g and 42%, increased seed yield, and lower installation cost.

Background : Ginseng (Panax ginseng C. A. Meyer) is a perennial crop grown for more than 4 years in the same place. Therefore, it is highly affected by soil environment, especially nutrients on soil. This study was carried out to investigate the effects of application levels of nitrogen, phosphate, potassium on growth and soil chemical properties of ginseng. Methods and Results : 0, 1, 4, 8, 16, 32 ㎏/10 a of Urea, fused superphosphate, potassium chloride were each treated at 3.3 ㎡ of the field and ginseng cultivar 'Geumpoong' was transplanted. Growth characteristics were investigated by growth period and soil chemical properties were investigated every 3 months. As the levels of treated nitrogen increased, root weight increased from 0 ㎏/10 a to 4 ㎏/10 a and then decreased. Electrical conductivity (EC) and the NO3 content tended to increase as time goes by. As the levels of treated phosphate increased, the SPAD content increased. Soil analysis of October showed that the NO3 content of phosphate 32 ㎏/10 a treatment increased to 80.25 ㎎/㎏. In the case of potassium 32 ㎏/10 a treatment, the emergence rate was 74% and root weight was 2.06 g, which were the worst in all treatments. When the levels of treated potassium increased, the EC tended to increase gradually and the pH was decreased. Conclusion : These results indicate that the application levels of Nitrogen, phosphate, potassium influence growth of ginseng and soil chemical properties. Further research will be needed to establish appropriate standards of soil chemical properties for ginseng cultivation.

Background : The geographical origin of Panax ginseng Meyer, a valuable medicinal plant, is important to both ginseng producers and consumers in the context of economic profit and human health benefits. We therefore aimed to discriminate between the cultivation regions of ginseng using the stable isotope ratios of C, N, O, and S, which are abundant bio-elements in living organisms. Methods and Results : The C, N, O, and S stable isotope ratios were measured by isotope ratio mass spectrometer, and then these isotope ratios profiling was statistically analyzed with chemometrics. The various isotope ratios found in Panax ginseng roots were significantly influenced by region, cultivar, and the interactions between these two factors (P ≤ 0.0002). In particular, δ18O was lower in ginseng roots grown at high altitudes (r = −0.47), while δ34S was higher in ginseng roots grown close to coastal areas (r = −0.48). Chemometric results provided discrimination between the majorities of different cultivation regions. Conclusion : Our case study extends the understanding about the variation of C, N, O, and S stable isotope ratios in ginseng root depending on cultivation region. Hence, the analysis of stable isotope ratios is a suitable tool for discrimination between the regional origins of ginseng samples from Korea, with potential application to other countries.

Background : Although ginseng has various bioactive compounds in it, there is lack of study on the variations of bioactive compounds in ginseng according to the cultivation soil and the applied fertilizer types (or amount). Therefore, this study aims to examine the variations of 37 fatty acids (FA) and 8 vitamin E (Vit-E) vitamers in 6-year-old ginseng root cultivated in different soil types with different fertilizers regimes. Methods and Results : The profiling of 37 FAs and 8 Vit-E vitamers in 6-year-old ginseng roots was measured by gas chromatography coupled with a flame ionization detector, and then these results were statistically analyzed with chemometrics. The FA and Vit-E content in ginseng roots varied significantly with respect to soil cultivation conditions due to organic fertilizer types and amounts used. Unsaturated FA in ginseng is approximately 2.7 fold higher than the saturated FA. Linoleic, palmitic, and oleic acids were the most abundant FAs found in the ginseng roots. Also, the major Vit-E vitamer found in ginseng root is α-tocopherol. In particular, the application of rice straw compost or food waste fertilizer was increased to create nutritionally desirable FAs and bioactive Vit-E in ginseng root. In addition, phytonutrient profiling coupled with chemometrics can be used to discriminate the cultivation conditions of ginseng. Conclusion : This study extends our understanding about the variations of FA and Vit-E in ginseng root depending on cultivation conditions. Hence, these results can be useful as basic information for reliable ginseng production containing high amounts of phytonutrients in a paddy-converted field.

Background : Ginseng is becoming depleted of virgin cultivation area due to the problem of replant failure. Ginseng farmers have become more burdensome in operating expense because they are more likely to go out to other cities in search of virgin cultivation area. In addition, the quality and yield of ginseng cultivated in one place for many years depend on the rapidly changing climate every year. The purpose of this study was to develop a method for continuous production of ginseng in a facility by solving the problems of replant failure and investigating basic soil composition and growth characteristics of ginseng for 2 - 6 years. Methods and Results : This study was carried out in a 90 ㎝ wide, 50cm deep and 22 m long bed made of sandwich panels in a 90% shaded facility for ginseng cultivation. In the lower part of the bed, a 100 ㎜ pipe for drainage and steam sterilization was installed, and the pearlite was filled at a height of 100 ㎜ as a drainage material. The soil for ginseng cultivation was put into the bed. Soil composition was tested in five combinations including virgin soil, yokto, peat moss, pearlite, and vermiculite with different composition ratios including control. The native seedlings were transplanted and grown from 2 years to 6 years. In the growth characteristics and yield of ginseng, the best treatments were virgin soil 55%, yokto 10%, Peatmoss 25%, Perlite 5% and 5% vermiculite. Also, the bulk density was reduced by 30% compared to the control. Soil pH and EC tended to increase slightly during all treatments. In the ginsenoside analysis, there were no unusual results for the soil composition and they were almost similar. Conclusion : As a method to continuously grow ginseng in the facility, we tried to grow ginseng by filling the soil in the bed. Soil composition should be within the range of chemistry and physics suitable for cultivation of ginseng, and it is necessary to analyze the economy and reduce the operating expense. In the future, researches on soil disinfection and nutrient management methods for continuous use should be continued.

Background : Sorghum is a major cereal food crop used in many parts of the world. It has been grown on a subsistence level by farmers, under various conditions of environmental stresses in the semi-arid tropics of Africa and Asia. This crop has received significant attention because of its ability to lower cholesterol in the blood, and its anti-dementia, antioxidant, and antimicrobial properties. It is possible to develop a functional and commercially viable sorghum variety by using superior cultivars of sorghum. The objective of this study was to construct a database of superior sorghum accession. Methods and Results : We used 250 sorghum accessions collected from different geographical bioregion in Korea. We determined various growth characters including germination rate and ear length of these accessions. To determine the antioxidant capacity, we measured the DPPH radical scavenging activity, ABTS scavenging activity, total phenolic contents, and total flavonoid contents. 189 accession showed higher germination (> 80%) than other accessions. Higher DPPH radical scavenging activity was observed in 11-SB-078 (RC50: 1.89 ± 2.88 ㎍/ ㎖), and higher ABTS scavenging activity was recorded in 11-SB-116 (RC50: 35.48 ± 2.42 ㎍/㎖) than other accessions. The ear length ranged from 15 ㎝ to 48 ㎝, the total phenolic contents ranged from 3 ㎎·GAE/g to 77 ㎎·GAE/g, and total flavonoid contents ranged from 0.09 ㎎·QE/g to 1.07 ㎎·QE/g. Conclusion : Among 250 sorghum accessions, we selected 10 with both superior agronomic characters and higher functional food quality.

Background : This study aimed to investigate the effect of elevated temperature by climate change on growth and ginsenoside contents of Korean ginseng, which requires low temperature during growth period. Methods and Results : Yunpoong was transplanted in temperature gradient chamber (TGC) for high temperature treatment and then growth characteristics and ginsenoside contents were investigated by growth period. Stem length, petiole length, leaf length and leaf width were increased as the temperature was higher than Ambient Temperature (Amb), but stalk diameter was decreased and small petiole length, number of petiole and leaf were no significant difference. Root growth, whereas, including length diameter and weight were decreased as the temperature was higher than Amb. Compared with Amb, root length, diameter and weight were decreased by 19.6%, 22.4% and 41.5% at Amb+6, respectively. The higher the temperatures are associated with the more ginsenoside contents. Compared to Amb, total ginsenoside contents were increased by 68% and 78% at Amb+4℃ and Amb+6℃, respectively. Almost all contents of individual ginsenoside, with the exception of Rg1, made a significant difference. Conclusion : When temperatures rises during growth period, the growth of ginseng was decreased but, ginsenoside contents were increased.

Background : Panax ginseng C.A. Meyer is a representative medicinal plants and it has been used in traditional medicine because the plant have many effective component such as saponins. To obtain a wild-cultivated ginseng (WCG) which is similar to wild ginseng (WG), this method usually performed in a mountain using seeds or seedlings of cultivated ginseng (CG) and WG. WCG is very expensive because it is difficult to cultivate. However, systematic cultivation method have not been developed compared to their high added value. Furthermore, very high price of WCG caused the problem that Panax notoginseng or Panax quinquefolium are sold as WCG in Korean market. This is concerned as a serious problem to consumers. In this study, we analyzed the agronomic and growth characteristics of the WCG cultivated in Korea. Methods and Results : We examined the WCG that was collected and sold by regional groups at the Korean market. The root age, growth conditions, and quality level of the cultivated WCG were confirmed. WCG samples were collected from five areas in Korea (Bucheon, Cheongju, Hoengseong, Judeok and Ulsan). The main root diameter, root shape index, rhizome length, and root weight showed high level of variation and they did not form annual rings. Conclusion : Agronomic and growth characteristics of WCG showed high variations according to cultivating regions.

Background : Wild-cultivated ginseng (WCG) prices are very different according to root ages. Generally, two methods are used to discriminate the root ages of Panax ginseng C.A. Meyer. The first method is the yearly determination method by the ring dyeing method, and the second method is the confirmation the number of stem vestiges in the rhizome. In this study, we analyzed the agronomic and growth characteristics of the WCG cultivated in Korea. In this study, to determine the appropriate root ages discrimination method for the determination of the root ages of WCG, the root ages of WCG and cultivated ginseng was examined. Methods and Results : We examined the cultivated ginseng (CG) and WCG that was collected and sold by regional groups at the Korean market. WCG does not form annual rings, which are clear and regular in wild ginseng. Therefore, it is impossible to identify the age of WCG by using the annual growth rings staining method. However, the age can be estimated by determining the number of stem vestiges in the rhizome. Conclusion : From the results of the Study on identification of root age for quality evealuation in WCG in Korea. Appropriate root ages discrimination method of WCG was confirmation the number of stem vestiges in the rhizome.

Background : For the GAP standard cultivation, weed occurrence pattern was investigated by planting density and mulching method of Bupleurum falcatum L. Methods and Results : Vinyl and rice straw were used for mulching. The planting density were 10, 20, and 30 cm, and the spacing between plants were 5 ㎝. The amount of weed emergence were examined twice at the end of July and at the end of September. The degree of importance was based on the Braun-Branquet’s dominance value distinction criterion. As a result, the kind of total weeds emerged during the cultivation of Bupleurum falcatum L. were identified as 20 species in 10 families. Amount of weed’s occurrence were the highest in rice straw covering after 10 × 5 ㎝ sowing, and it were lowest in vinyl mulching after 30 × 5 ㎝ sowing. The frequency of weed emergence was highest with 10 species of 8 families in rice straw mulching treatment. On the other hand, the treatment with the least emergence frequency of weeds was 30 × 5 ㎝ sowing after vinyl mulching, and weeds of 7 species of 6 families appeared. In the second survey in September, weeds of 15 species in 9 families were identified in the test field. The amount of weed’s occurrence were the highest in rice straw mulching after 10 × 5 ㎝ sowing, and it were the least in the case of vinyl mulching after 30 × 5 ㎝ sowing. The frequency of emergence of weeds was highest in rice straw mulching after 20 × 5 ㎝ sowing, and the kind of weeds were 11 species of 8 families. The treatment of the lowest emergence frequency of weeds was 30 × 5 ㎝ sowing after vinyl mulching. Three kinds of weeds occurred. Conclusion : From the above results, it is expected that we can suppress the weed occurrence at the planting density of 30 × 5 ㎝ after vinyl mulching at the sowing of Bupleurum falcatum L.

Background : This experiment was conducted to select suitable industrial crop after Panax ginseng cultivation under the greenhouse in the middle area. Methods and Results : This experiment was conducted in greenhouse of Chungbuk Agricultural Research Service and extension services from 2015 and 2016 years. Ginseng grown four years in greenhouse. After cropping of Panax ginseng was transplanted Cynanchum wilfordii, Platycodon grandiflorm, Dioscorea opposita, and Codonopsis lanceolata. Non-cultivated field that were grown sesame. Cynanchum wilfordii, Platycodon grandiflorm, and Codonopsis lanceolata was collected in the Chungbuk area and Dioscorea opposita was collected in Andong-si. Each industrial crop after panax ginseng cultivation under the greenhouse was sowing from late April to early May 2015 years. The growth and yield of crop examined in the experiment were good on the whole except Codonopsis lanceolata without disease due to continuous cropping of Panax ginseng. The yield of Cynanchum wilfordii, Platycodon grandiflorm, Dioscorea opposita, and Codonopsis lanceolata was 803kg, 1,393kg, 1031kg, 412kg per 10a respectively, and the every decline yield index was by 1%, 8%, 12%, and 31% respectively. The proper crop in after cropping of Panax ginseng was cynanchum wilfordii, which was yielded 1,393kg per 10a by fresh tuber and it was 1% decline compared to the non-cultivated field. Conclusion : In this study, Cynanchum wilfordii, Platycodon grandiflorm, Dioscorea opposita was recommended as a crop after Panax ginseng cultivation under the greenhouse in the middle area.

Background : Cudrania tricuspidata Bureau is a widely used medicinal perennial woody plant. Obtaining information about the genetic diversity of plant populations is highly important for conservation and germplasm utilization. In this study, we developed single nucleotide polymorphism (SNP) markers derived from chloroplast genomic sequences to identify distinct Korean-specific ecotypes of C. tricuspidata via amplification refractory mutation system (ARMS)-PCR analyses. We performed molecular authentication of twelve C. tricuspidata ecotypes from different regions using DNA sequences in the chloroplast TrnL-F intergenic region. Methods and Results : SNPs were identified based on the results of nucleotide sequence for the intergenic region of TrnL-TrnF gene (chloroplast). Molecular markers were designed for those SNPs with additional mutations on the second base from SNPs for amplification refractory mutation system-polymerase chain reaction (ARMS-PCR). HRM pattern analyses were performed using the Mx3005P QPCR System (Agilent Technologies, CA, USA). Conclusion : We collected 12 individual lines of C. tricuspidata from various region in South Korea and China. Based on the nucleotide sequence in the trnL-trnF intergenic region of these lines, six SNPs and a deletion of 12 bps were identified and 12 individual lines were able to be grouped in one Korean ecotype and two different ecotypes of chinese lines, chinese line 1 and 2. The SNP markers developed in this study are useful for rapidly identifying these specific C. tricuspidata ecotypes collected from different regions.

Background : Cucuma longa L., in the family Zingiberaceae, is distributed in tropical and/or sub-tropical regions mainly in India and China. This species is commonly called tumeric, powder is used as medicinal herbs and/or flavor enhancer. It has been cultivated in southern region mainly Jindo. However, it might be possible to extend cultivation region due to rise in average temperature. In order to select superior lines, agronomic characteristics is commonly used. Because this is not the ultimate solution, the DNA marker approach has benefited the modern plant breeding. Therefore an easy approach by using one kind of primer have been developed from random amplification of polymorphic DNA sequences (RAPD) to discriminate effectively between different cultivars of Cucuma species Methods and Results : DNAs were extracted from the harvested roots of Cucuma sp. using DNeasy plant Mini kit (Qiagen, Hilen, Germany). These plants cultivated from GARES (Hamyang) and used for PCR amplification. The relative concentration of the extracted DNA was estimated Nano Drop ND-1000 (NanoDrop Technologies, Wklmington, De, USA) and final DNA concentration was adjusted to 5.5 ng/㎕. In this study 9 primer pairs were tested on 8 Cucuma sp. These primers showed polymorphism in Cucuma sp. The cluster dendogram showed that the similarity coefficients ranged from 0.68 to 0.87, CUR02 turned out to be CUR11, and CUR16 is similar to CUR17. Conclusion : These finding could be used for further research on cultivar development by using molecular breeding techniques and for conservation of the genetic diversity of Cucuma species. These data on polymorphism difference based on RAPD will be give us invaluable breeding information by selection of superior lines.

Background : For cultivation of varieties of ginseng, the pure line selection method, which is to select the best among those cultivated in farms for pedigree breeding, replicated yield trials and regional adaptation trials before registering as a new variety, is widely used. Although there are 25 registered varieties of ginseng in Korea, the quality of ginseng is declining together with the amount of harvest being decreased by 15 - 20% due to the heat injuries and diseases from the warming & abnormal climate. Thus, the needs for development of disaster-resistant varieties with better chances of surviving through high temperature, salts and disease are increasing. Therefore, this study is to cultivate disaster-resistant varieties among those selected for their disaster tolerance and salt tolerance through regional adaptation trials. Methods and Results : As a result of examining the growth characteristics of the selected 2 - 5 year old varieties used in the study, among the 5-year old crops, Goryeo 4 and Eumseong 5 showed superior growth in both above and below aerial parts, and among the 4-year old crops, Eumseong 11 and Cheonryang showed superior growth while the growth in the below aerial parts were satisfactory in the order of Cheonryang > Eumseong 10 > Eumseong 11 > Eumseong 9. Among the 3-year old crops, the most superior growth in both above and below aerial parts was observed in Eumseong 14 with the weight of the below aerial part, root diameter and taproot length at 13.8 g, 11.8 ㎝ and 6.2 ㎝ respectively. Among the 2-year old crops, Eumseong 10 showed the most superior growth in both above and below aerial parts. Conclusion : Based on the above results, Goryeo 4 and Eumseong 5 among the 5-year old crops, Eumseong 11 among the 4-year old crops, Eumseong 14 among the 3-year old crops and Eumseon 10 among the 2-year old crops showed the most superior growth among the selected varieties. The growth characteristics of both above and below aerial parts in each year will continuously be monitored.

Background : Chrysanthemum indicum L. is used in medicine, for bee, ornamental as multivoltinism plant resources. Since ancient times, Chrysanthemum indicum L. had been widely used as drug and tea. To diversity and increase the demand of Chrysanthemum, it is breeding for medicinal herb, tea and ornamental flower. Therefore size and shape of plant, color of flower have been used for cross breeding. CI2013 lines collected from Jeju have been used for landscape and ornament. and CI2010 lines collected from Sancheong have been used for medicinal herb tea. As a result, many new lines were developed. Therefore it was carried out to use as a date for new varieties through the characterization of good lines. Methods and Results : 7 lines were investigated and these plants were forstered and cultivated from Gyeongsangnam-do Agricultural Research & Extension Services (Hamyang). Breeding Lines were CI201001, CI201002, CI201005, CI201006, CI201303, CI201307, CI201401. The shape of CI201303 and CI201307 were round, plant height is low and width is wide, it may be used for landscape and ornament. As the size of flower in CI201005 was big, it may be used for medicinal herb tea. Conclusion : CI201303 and CI201307 lines were good for landscape and ornament because of round shape. CI201005 line was good for medicinal herb tea because of big flower.

Background : Licorice has been used as a source of medicine and a food material in East-Asia. Recently, demand for licorice increased in market due to a growing interest in health. Thus we conducted breeding research to solve the problems associated with domestically cultivated licorice such as low productivity and low glycyrrhizin content. Methods and Results : We crossed European licorice (G. glabra L.; female parent) and Chinese licorice (G. uralensis Fisch; male parent) in the greenhouse in May 2007. In September 2007, crossed and germinated seeds were retrieved and sown in the greenhouse. In June 2008, stolons were separated from the F1 licorice seedlings and cultivated, resulting in 32 clonal lines of interspecific hybrids. Among them we selected good lines and then conducted the replicated yield trials (RYT) in 2012-2013 and local adaptability test (LAT) in 2014-2015. The results, GLYES9 showed that was elect of stem, oblong of leaf shape, red-brown of root color. Glycyrrhizin conten of GLYES9 (3.0%) was higher than G. uralensis (1.9%) at four regions from 2014 to 2015. GLYES9 was less than 10% in the desease of brown spot (G. uralensis was more than 30%). The root yield of GLYES9 was 4.31 ton per hectare, which was increased 193% compared with a check variety of G. uralensis. Therfore, we named GLYES9 as new cultivar ‘Dagam’. Conclusion : Depending on the above results, we have developed a new licorice cultivar ‘Dagam’ by the medicinal crop breeding team of National Institute of Horticulture and Herbal Science, RDA, in 2015. It showed brown spot disease resistant, high-glycyrrhizn content and high-yielding than colleted Glycyrrhiza spp.