Background : Radish sprouts or young seedlings are important nutritional vegetables in Asian countries. In the present study, we investigated plant growth and levels of glucosinolate accumulation in radish sprouts in response to treatments with different carbon sources. Plant growth and accumulation of glucosinolates were inversely correlated in treatments with different carbon sources. Methods and Results : All the carbon sources used in this study inhibited the growth of shoot and root in radish sprouts and facilitated significantly the accumulation of glucosinolates. Seven different glucosinolate compounds were detected in radish sprouts treated with different carbon sources. The total as well as individual amounts of all the identified glucosinolates increased after treatments with different carbon sources, except for 4-methoxyglucobrassicin and glucoiberin. In particular, the supplementation of sucrose, galactose, and glucose resulted in highest glucosinolate accumulation in radish sprouts. Radish sprouts treated with sucrose showed the highest levels of total glucosinolates, which was 1.22-fold higher than that of untreated sprouts. Furthermore, sucrose treatment resulted in higher production of gluconapoleiferin, glucoerucin, glucoraphasatin, and glucobrassicin compared with that in untreated sprouts, and the treatment of galactose and glucose similarly enhanced glucosinolate production when compared with untreated sprouts. Conclusion : seven glucosinolates were identified by HPLC and great differences in glucosinolates levels were observed in radish sprouts in response to different carbon sources. The glucosinolate production was positively affected by most of the stimuli used in this study. The results presented herein provide information about optimal cultivation conditions, particularly the suitable carbon sources, that will enhance glucosinolate production in radish sprouts.

Background : Fagopyrum esculentum Moench (common buckwheat) is an important pseudocereal due to high agricultural and medicinal values. It contains various minerals, fiber, and flavonoids. Additionally, flavonoids in buckwheat have various health effects. Thus, this study is aim to optimize the concentration of chitosan, salicylic acid (SA), and jasmonic acid (JA), for the production of phenolics in germinated buckwheat using high-performance liquid chromatography (HPLC). Methods and Results : The treatment with 0.1% chitosan increased the accumulation of all 7 phenolic compounds compared with the control, 0.01 and 0.5% chitosan treatments (p < 0.05). Furthermore, the germinated buckwheat treated with JA at the specific concentrations of 50, 100, and 150 μM increased the accumulation of total phenolic compounds. The germinated buckwheat grown in 150 μM of JA showed the highest amount of total phenolics which was approximately 2.47 times higher than that of control. Particularly, the accumulation of gallic acid, rutin, catechin, chlorogenic acid, and (-)-epicatechin were approximately 2.00, 2.38, 1.76, 2.81, and 7.95 times higher in JA-treated buckwheat than in the control buckwheat samples. A total of seven phenolics, including gallic acid, catechin, chlorogenic acid, caffeic acid, (-)-epicatechin, benzoic acid, and rutin, were detected in germinated buckwheat. Apparently, JA and chitosan treatment enhanced the accumulation of phenolic compounds in the germinated buckwheat. Particularly, the treatments with 0.1 % chitosan and 150 μM JA were the most effective on the accumulation of phenolic compounds. According to the time-course analysis, a 72 h chitosan treatment enhanced the production of phenolics. Similarly, the germinated buckwheat treated with 48 and 72 h showed the accumulation of higher levels of phenolic compounds than the control buckwheat. Conclusion : This study aimed to optimize the concentrations and treatment period of elicitors, chitosan and JA, for the enhanced production of phenolic compounds in germinated buckwheat. Thus, these results might help build sturdy strategies to enhance the production of phenolics in germinated buckwheat as a good nutritional source for human consumption.

Background : Light-Emitting Diodes (LEDs) have been reported to alter the composition of the secondary metabolites present in many plants. For example, light exposure has been reported to significantly affect secondary metabolite biosynthesis in plants, and irradiance levels have been reported to affect the concentration and production of both phenylpropanoids and carotenoids. Therefore, the objective of the present study was to determine the most effective LED light source in enhancing growth and secondary metabolites (polyphenols and carotenoids) and investigate the effect of LED illumination on production the of primary and secondary metabolites (polyphenols and carotenoids) in Vigna unguiculata L. Walp. sprouts Methods and Results : In order to determine the effect of light-emitting diodes (LEDs) on plant metabolism, the present study examined the primary and secondary metabolite profiles of Vigna unguiculata L. Walp. sprouts that were exposed to red, blue, white, or a combination of red and blue LEDs using high-performance liquid chromatography (HPLC), electrospray ionization-mass spectrometry (ESI-MS), gas chromatography-mass spectrometry (GC-MS), and gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). A total of 39 hydrophilic compounds were identified and quantitated using GC-TOF-MS, and six phenylpropanoids and six carotenoids were quantified using HPLC. The plants grown under blue LED light contained the highest level of total carotenoids (253.72 ± 17.27 ㎍/g) and phenylpropanoids (2,600.51 ± 4.90 ㎍/g). Thus, the current study provides a new approach for enhancing the carotenoid and phenylpropanoid production of V. unguiculata. Conclusion : This study suggests that blue LED light source is the most appropriate for the sprout growth and production of phenolic compounds and carotenoids in cowpea sprouts. Furthermore, these findings confirm that HPLC, GC-MS, and GC-TOF-MS are suitable for investigating metabolic relationships and offer a tenable strategy for enhancing secondary metabolite production using LED light sources.

Background : Basil is well known for its use in cooking, however, the herb is also noted for its value in traditional medicine. Basil is a digestive stimulant with antimicrobial, antibacterial, anticonvulsant and anticarcinogenic properties. Basil contains high levels of phenolic acids that contribute to its strong antioxidant capacity and the substantial concentrations of rosmarinic acid, in particular, have been associated with the herb’s medicinal qualities. Rosmarinic acid is noted in the literature to be the most prevalent basil phenolic but other caffeic acid derivatives, such as chicoric acid, are also found in high concentrations. But, no previous study about other phenolic compounds accumulation except previously mentioned. Thus, the aim of this study is to explain that basil accumulates other phenolic compounds except previously mentioned. Methods and Results : 6 basil cultivars, ‘Holy’, ‘Lemon’, ‘Cinnamon’, ‘Dark Opal’, ‘Bush’ and ‘Genovese’, were grown at experimental field of Chungnam National University. These plants leaves were freeze-dried at -80℃ for 72 h and then ground into a fine powder using a mortar and pestle. 100 ㎎ of the sample was weighed into 15 ㎖ tube and 2 ㎖ of 80% (v/v) methanol was added. Next, the tube containing the powder was allowed to be sonicated for 1h at 35℃, and then centrifugation was performed at 12,000 rpm for 10 min. The supernatant was filtered with a 0.45 ㎛ PTFE syringe filter and analyzed by a Futecs model NS-4000 HPLC apparatus. 12 phenolic compounds were identified and quantitated in the different cultivar leaves of basil through comparison of retention time, spike tests and external standard calibration curves using HPLC. Gallic acid, 4-hydroxybenzoic acid, catechin hydrate, chlorogenic acid, caffeic acid, p-coumaric acid, ferulic acid, benzoic acid, rutin, trans-cinnamic acid, quercetin, kaempferol were identified. Among 6 basil cultivars, ‘Dark Opal’ show the highest accumulation of phenolic compounds, particular benzoic acid (6.32 ㎎/g dry weight), rutin (1.13 ㎎/g dry weight) and 4-hydroxybenzoic acid (0.4 ㎎/g dry weight) are higher than other cultivars. Conclusion : The results presented herein provide information about variation of phenolic compounds in 6 basil cultivars, ‘Holy’, ‘Lemon’, ‘Cinnamon’, ‘Dark Opal’, ‘Bush’ and ‘Genovese’.

Background : Angelica acutiloba is a perennial herb from the family Umbelliferous. The root was used as a substitute for the crude drug. As a by-product of making cheese from whey, it has many proteins and nutritional components, but it has a drawback in that it must be stored in a refrigerator because the storage facility is short. In order to solve such problems, an attempt is made to solve the environmental and economic problems by producing a liquid fertilizer fermented with a large amount of whey. Methods and Results : In this study, we studied the fermentation of wheat germ with lactic acid bacteria (Lactobacillus brevis) and yeast (Saccharomyces cerevisiae). The preliminary experiment was conducted to determine the test concentration of 300 fold dilution and 500 fold dilution. As a result, the application of 300 times dilution liquid prepared by fermentation of whey using lactic acid bacteria showed higher growth and higher growth rate with higher number of leaves and branches. Growth response of the 500 fold dilution was higher than that of the control, but the growth of 300 fold dilution showed the highest. Conclusion : Therefore, it is considered that the 300 fold dilution of Lactobacillus can be used as a substitute for compost when cultivating medicinal plants.

Background : Sorghum (Sorghum bicolor) is a perennial plant of the Gramineae, followed by rice, wheat, corn and barley, the fifth major food crop in the world. Sorghum is mostly used in Korea for cereals using seeds or for making food directly. However, sorghum is not directly used for food in foreign countries, but plays an important role in the development of cosmetic and pharmaceutical materials and as a bio-energy crop. Most of the sorghum cultivated in Korea does not use the remainder after harvesting seeds, so research on other uses is needed. Therefore, we carried out this experiment in order to identify the characteristics of domestic genetic resources and to select the genetic resources with excellent growth. Methods and Results : Samples were tested for their growth characteristics by the Agricultural Genetic Resources of National Institute of Agricultural Sciences. Growth characteristics of heading, total length, stem length, stem width, leaf length, leaf width, length of ears and width of ears were investigated. After 10 weeks of sowing, 45% of the outbreaks occurred and showed a quick heading. The total length of the sorghum ranged from 65.7 ㎝ to 341.0 ㎝, and the stem width ranged from 0.2 ㎝ to 2.6 ㎝. Leaf length ranged from 33.3 ㎝ to 92.4 ㎝ and leaf width ranged from 3.8 ㎝ to 12.2 ㎝. The length of the ears was in the range of 8.0 ㎝ - 52.7 ㎝, and the width of the ears was in the range of 5.2 ㎝ - 27.0 ㎝. K131301, K178840, K258654 and K255198, which are expected to bear a large number of seeds with high biomass content and large size of leaves and plants, were selected. Conclusion : Through this study, we can understand the growth characteristics of the sorghum in Korea and select excellent genetic resources.

Background : Ixeris strigosa is a herb which has used leaves and roots in Korea. Recently, the cultivation area and food imports have been increased because it has been used as an functional crops for anti-obesity. However its cultivation method has not been developed and it is difficult to produce with good quality. Therefore, this experiment was carried out to determine the optimum seedroot size, plant number per hill, planting time, planting density, planting method and mulching materials for improving the cultivation method suitable for domestic environment. Methods and Results : In order to identify the proper cultivation method, I. strigosa was collected in Dangjin, Chungnam in 2016 and the yield and crop characteristics were investigated at the end of October. The seedroots were cultivated by size such as large (length 30 ㎜, thickness more than 1 ㎜), medium (30 ㎜, 1 ㎜ - 1.5 ㎜) and small (30 ㎜, less than 1 ㎜). As a result, the production of the medium-sized seedroot was highest at 950 ㎏/10a. Based on these results, medium-sized seedroots passed winter in previous year were used when plant number per hill, planting time, planting density, planting method and mulching materials were investigated. At plant number per hill, 3 seeds/hill was the highest at 836 ㎏/10a among 1, 3 and 5 seeds/hill. When the seedroots were planted at intervals of one month from mid April to mid August, the yield was the highest at 1,647 ㎏/10a at the middle of May. When the planting densities were 10 × 10, 20 × 10, 20 × 15 and 20 × 20 ㎝, 10 × 10 and 20 × 10 ㎝ had highest production. And two methods for planting have been used, one was to plant at right angles and the other to lay down. As a result, the latter yield was 612 ㎏/10a. Finally, when comparing non woven fabrics, color combination polyethylene film, black polyethylene film and no treatment among the mulching materials, black polyethylene film made the highest yield at 1,044 ㎏/10a. Conclusion : For improving the cultivation of I. strigosa, this study determined the optimum seed size, plant number per hill, planting time, planting density, planting method and mulching materials. In conclusion, the production was the best when the plant was laid down on a black polyethylene film at intervals of 20 × 10 ㎝ at the middle of May, using 3 medium-sized seeds per hill. These results are expected to contribute to the productivity and income farmers of I. strigosa.

Background : Atractylodes macrocephala is a perennial herbaceous plant belonging to the family Asteraceae and should be cultivated in field soils with good water dripping due to plant characteristics. However, cultivating farmers mainly have recently been cultivated in paddy soil due to their regional characteristics, which causes the decrease in yield due to poor drainage. Therefore, this study was carried out to investigate the cultivation in high ridge and subsoil breaking effect for stable paddy soils cultivation technology of A. macrocephala. Methods and Results : Soil was paddy soils in the fall of 2017, and the pH (1 : 5) was 6.61 ± 0.15 as a result of chemical and physical properties. EC was 0.49 ± 0.05 dS/m, and organic matter content was 28.69 ± 69 g/㎏ and effective phosphoric acid was 306 ± 17.8 ㎎/㎏. As a result of the soil layering survey, the surface layer was 0 - 26 ㎝ deep as paddy soil mounded with sandy loam in the past. In the surface layer, there was a light layer after 17 ㎝ depth, and volume density was 1.71 ± 1.3 g/㎤. The porosity of the plow pan was 33.41 ± 2.34%. The cultivation methods were cultivation in high ridge (30 ㎝ or more) and level row (10 ㎝ or less) at 2 levels and 3 repetitions. In subsoil breaking, the depth of the plow pan was increased from 17.1 ± 0.5 ㎝ before treatment to 31.1 ± 3.6 ㎝ after treatment and the hardness was 24.8 ± 1.5 ㎜. In the case of rotary plowing, the depth of the plow pan was 17.1 ± 1.9 ㎝ before treatment and 26 ± 2.4 ㎝ after treatment and the hardness was 25.8 ± 2.9 ㎜. The medium growth characteristics of A. macrocephala per treatment showed the tendency of increase in plant length, culm length, number of nodes, number of leaves, and fresh weight in level row cultivation after subsoil breaking. Root growth of cultivation in high ridge after subsoil breaking tended to be good with rhizome weight of 11.6 g per hill. The survival percentages were 98.8 - 100% and the bolting rate was 93.4 - 96.2% Conclusion : In cultivation in high ridge after subsoil breaking in the paddy field of Gangwon area, the decrease of yield of A. macrocephala due to drainage was expected to be alleviated, but final conclusion should be drawn after analyzing soil temperature and soil moisture data.

Background : This study was carried out to introduce Atractylodes macrocephala as a new income element in Gangwon area and to develop the technology necessary for stable quality seedling production. Methods and Results : For the production of high quality seedlings of Atractylodes macrocephala, seedling growth characteristics were investigated according to the types of plug cell size and seedling raising period. Atractylodes macrocephala seeds were sown on February 14, 2018 in 72, 105, 128, 162, and 200 plug trays. The emergence period after sowing was March 2, and the final occurrence rate was 76.6 - 79.5%. The number of days of emergence took 18 to 20 days from sowing date. Growth of seedlings tended to be better with less number of plug trays, such as seedling height, seedling width, leaf length, leaf width and leaf number. On the other hand, roots (net formation) increased rapidly as the number of plug trays increased. After 60 days, the matured seedling rate was good at 75.5 ± 8.4% for the 200 plug tray and 72.5 ± 4.1% for the 162 plug tray. The net formation ratio of matured seedling was the best in 60 days of seeding in 162 plug trays. The rooting rate was 98.0 ± 2.1 - 99.3 ± 1.2% when seeded for 60 days or more regardless of the type of plug tray. Conclusion : In order to produce efficient and stable seedlings in the cultivation of Atractylodes macrocephala in Gangwon area, it was considered to be advantageous for 60 days of seedling settling in the plug trays of more than 162 and less than 200.

Background : This study was conducted to investigate the effect of soluble silicate and germanium on the characteristics of soil and growth in ginseng (Panax ginseng C. A. Meyer) cultivated in blue-white plastic houses. Methods and Results : Seedlings (n = 63 per 3.3 ㎡) of Korean ginseng cultivar were planted on April 10, 2015. Shading material of plastic house [8 m (W) × 3.5 m (H) × 50 m (L)] was blue-white vinyl. From May to September of each year, 1,000 times diluted sol. of germanium and silicate and these mixed solutions were drenched once a month. The chemical properties of the soil are as follows : pH, EC, organic content, and P2O5 of the soil were within the proper range, and the Mg content of the Ex-cations was lower than the standard value in 3 treatments except treatment of germanium 1,000 times. The total nitrogen content was 0.13 - 0.14%, which was higher than the optimum value. The number of soil microorganisms (Actinomycetes etc.) was higher in the treatment than in the non-treatment of inorganic components. The photosynthetic rate of ginseng leaves was 2.25 μmole CO2/㎡/s in the non-treatment and 2.91 to 3.17 μmole CO2/㎡/s in the inorganic treated. The growth characteristics of the aerial parts (leaf and stem) of the ginseng plants were generally better than that of non-treatment. Especially, in the treatment with silicate 1,000 times showed better growth, leaf area and leaf width growth. The growth of the underground part (root weight etc.) were also better than non-treatment. Specially, at 1,000 ppm treatment with soluble silicate, the root length and diameter were longer and the weight was was 72.2 g per plant. The ginsenoside content of each treatment is under analysis. The disease incidence (Alternaria panax etc.) rate was 20.1% in the untreated and 6.4% to 10.0% in the inorganic components treatment. Conclusion : By soil drench of inorganic compononts such as soluble silicate in ginseng blue-white plastic house cultivation, the growth of the aerial and root parts in ginseng were more improved than non-treatment. There are a lot of soil microorganism and less disease.

Background : Ginseng is an important agriculture plant in Korea. However, plant yield is reduced by pathogens. Cylindrocarpon destructans and Fusarium solani are responsible for root-rot and replant failure of ginseng in Korea. Because of root rot pathogen, the productivity decreased and repeated cultivation is difficult. Methods and Results : The number of Cylindrocarpon destructans and Fusarium solani in soils can be measured by real-time PCR. This methode makes it possible to select of land for caltivation of ginseng. The specific primers of C. destructans and F. solani were synthesized from β-tubulin region. The equation of the standard curve between the colony forming unit(cfu) and the Ct value in the C. destructans was y (Ct value) = -1.608X (cfu) + 39.325. The equation of the standard curve between the colony forming unit (cfu) and the Ct value in the Fusarium solani was y (Ct value) = -1.608X (cfu) + 39.077. This method makes possible to rapidly exactly measure the number of pathogens in soil. C. destructans, a ginseng root rot fungus, was detected in soil samples of 32 (16%) in soil samples. 35.5% of paddy field, 34.3% of paddy field, 64.1% of field, and 65.6% of paddy field were found in perennial plant. Conclusion : As a result, the major causative agent of ginseng root rot was Cylindrocarpon and the onset density was 102 cfu/g in soil. There was no significant difference in density between fusarium and disease.

Background : Turmeric is a subtropical plant, which has a high temperature for growing, but if it continues to be high temperature, it will be turned into heat degradation phenomenon. In order to mitigate damage caused by high temperature, we investigate the damage caused by high temperature. Methods and Results : This study was carried out in the experimental packaging (April 2018 seeding) of the Gyeongnam provincial medicinal resource research institute and eight systems of Turmeric (Curcuma longa) As the main research items, temperature characteristics of treatment distinction and high temperature damage rate were investigated. From July to August 2018, the main weather condition was over 35℃ for more than 21 days and showed abnormal high temperature. CUR11 showed the highest mortality rate (9.1%), and CUR10 (44.8%) showed the highest mortality rate. The average number of CUR11 strain was 23.2%. The mortality rate was 4.9% at the 60 th day of the emergence period and 40.2% at the 75 th day after 2 weeks. The higher the loss rate, the higher the damage rate. When the coating was maintained, the coating surface showed a high temperature of 50℃ or higher. A test was conducted to confirm the heat-reducing effect by coating, as a result of the test, there was no significant difference in the removal rate compared to the control. Conclusion : In order to alleviate the effect of high temperatures on the growth of turmeric, it was found that the heat loss caused by the coating did not appear much in case of turmeric.

Background : When the Platycodon grandiflorum is applied before the rainy season, the increase of the incidence of the Platycodon grandiflorum root rot disease increases greatly. This experiment was carried out to reduce the incidence of rot root rot disease through the lodging protecting method. Methods and Results : Three-years-old Platycodon grandiflorum was subjected to four treatments with no treatment, net installation, cutting stem, and removing bud. No treatment was used as a control, and as a further control, netting was used in the Platycodon grandiflorum to set up a treatment with almost no lodging. In the case of cutting stem, the stem was cut off in the middle of June, leaving more than 60 ㎝ before flowering. In the case of removing bud, blooming just before, the bud was removed. As a result, the coverage rate was the highest at 36.9% in the non - treatment area in the middle of July after the rainy season and 0.4% in the net installation. Compared with the case of cutting a lot of stems, 12.7% of the stem was covered with stones, whereas the stalk was 31.8%, which was close to the untreated stomach. As a result of the change of morbidity rate per treatment, it showed a morbidity rate of 49.7% in case of net installation, compared with 60% or more morbidity rate in case of untreated. Conclusion : As a result, an anti-lodging technic has helped prevent the onset of root rot disease. Further research on how to prevent the lodging of Platycodon grandiflorum using cutting stem will be needed.

Background : Rehmannia glutinosa is a perennial herb belonging to family Scrophulariaceae. This study was breeding of R. glutinosa cultivar with insect tolerance and high-yielding. Methods and Results : ‘Chunggang’ is developed by the medicinal crop breeding team of National Institute of Horticulture and Herbal Science (NIHHS), Rural Development Administration (RDA), during the period from 2005 to 2017. The reproduction of Rehmannia glutinosa has been accomplished mainly by vegetative propagation with its seedlings. This cultivar was selected from seedling of RG10. The plant type of Chungang is some rising from ground. Regional yield trials conducted at three site from 2016 to 2017. The root yield of ‘Chunggang’ was 22.3 ton per hectare, which was increased 11% compared with ‘Jihwang 1’ (check variety). Also, ‘Chunggang’ has higher insect tolerance compared with ‘Jihwang 1’. Conclusion : ‘Chunggang’ is a insect tolerance and high-yielding Rehmannia glutinosa cultivar.

Background : Astragalus membranaceus belonging to the family of Leguminosae have been utilized as a traditional medicine. The aim of this study is to elucidate the basic information for breeding to superior Astragalus membranaceus cultivar. Methods and Results : Selection lines were developed by the medicinal crop breeding team of National Institute of Horticulture and Herbal Science (NIHHS), Rural Development Administration (RDA). The root of A. membranaceus lines were harvested in late October 2017. Calycosin-7-O-β-d-glucoside and calycosin were anlyzed by HPLC. The root yield of six selection lines were increased 8.9 - 74.8% compared with ‘Aseong’ (check variety). Also, the plant height of seven selection lines were shorter than ‘Aseong’. The calycosin–7-O-β -d-glucoside content was higher in six selection lines than ‘Aseong’. The calycosin content was higher in all selection lines except 1 line (1508 - 03) than that of ‘Aseong’. Conclusion : Three superior lines with short height and high yield were selected. These superior lines will conduct advanced yield trial to make varieties.

Background : The study is investigated the development of high-quality standard variety, character of gene resources, growth and yield production to collecting varieties in Agastache rugosa O. Kuntze containing antioxidant agriculturally. Methods and Results : Character of gene resources investigated collecting 16 varieties (Jeonnam Naju 1, 2, 3 (NJ), Gwangyang 1, 2 (GY), Sooncheon 4 (SC), Boseong 1, 2 (BS), Yeosu (YS), Jangheung (JH), Jindo (JD), Gyeongnam Hamyang (HY), Gyeongbuk Bonghwa (BH), Chungbuk Eumseong (ES), Cheongju (CJ), Gangwon Cheolwon (CW). Method of cultivation were conducted under sowing dates of plant a seedling were April 24. Transplanting with Agastache rugosa O.K. seeds in June 27 by growing pot seedling for 60 days in 2017. Fertilization application were standard application (N-P-K-Compost applied at 12-16-10-600 ㎏․10a−1). N-K applied at 60% of basal fertilizer, 40% of top dressing were two times (N-K) application. Planting density were spaced 30 ㎝ apart in rows 20 ㎝ apart with with non-woven fabric mulching cultivation. Plot design randomized block 3 repetition. Conclusion : Endemic characteristics of gene resources to collecting variety in leaf color (light green), leaf pliable (softness) appeared 8 lineage, leaf color (deep green), the contrary leaf pliable (roughness) showed 8 lineage. Flower color bloomed in light purple 8 lineage flower deep purple color come into 8 lineage. Also, the period of maturity ripened Sept. 27 - 31. on 8 lineage (BS2, GY1, JD, HY etc.). 3 lineage (YS, CJ, CW) attained to maturity on Oct. 14 - 16. Growth of aerial part increased on long length, large width of leaf, heavy dried weight of leaf and weight of seeds 10 lineage SC4, GY1, 2, NJ2, 3, BS1, 2, CJ, ES, BH etc. but short length, small width of leaf, declined in light dried weight of leaf and weight of seeds 2 lineage YS, CW.

Background : This study examines the main growth characteristics, physiological stress, or diseases damage for 3 and 4-years-old ginseng in the new lines cultivated in Gyeonggi for development of new lines of Ginseng. Methods and Results : Eumseong 13, 14 and 15, which are two-years-old seedling in the new line ginseng were transplanted in 7 lines × 9 rows (70 plants)/1.62㎡ in 2015 along with Gumpoong and Yeonpoong as the control lines while Eumseong 16 and 17 as well as Chunpoong as the control line were transplanted in the same way in 2016. The ginseng shading was installed with the front post of 170 ㎝ high and the rear post of 100 ㎝ high using the rear line while as the sun blocking material, the aluminum shade plate was used. As for the new growth lines of 3 and 4-years ginseng in the new growth line, the aerial parts of the ginseng and underground part of ginseng were examined. In addition, the physiological stress such as the leaf discoloration, rusty root and root rot as well as the resistance against the disease were examined. It was found out that as for the aerial parts of 3-years-old ginseng in test line, Eumseong 16 and 17 were better in the growth than the control lines were while in 4 years old ginseng, Eumseong 13 and 14 were better in the growth than Gumpoong as the control line was. As for the root weight of the 4-years-old ginseng for the underground part, there was no significant difference compared with control line while in 3-years-old ginseng, Eumseong 16 and 17 were heavier. The leaf discoloration did not happen to both 3 and 4-years-old ginseng. As for the root rot, 3-years-old Eumseong 16 and 17 suffered less root rot in the same way as for the control line. 4-years-old Eumseong 14 and 15 had less root rot in the same way as for the control line. 3-years-old Eumseong 16 and 17 have less rusty root in the same way as the control line while as for 4-years-old ginseng, Eumseong 13, 14 and 15 showed a lot of rusty roots in the same way as the control line. Conclusion : Finally, considering the growth characteristics, physiological stress and root rusty of the aerial parts and underground parts of the ginseng, we selected Eumseong 16 and 17 as the excellent lines for 3-years-old ginseng.

Background : Boxthorn (Lycium chinense M. 2 n = 24) is a self-incompatibility crop of solanales. It can not be fertilized in the cultivation of single cultivars. Therefore it is necessary to select proper pollinizer. We developed a new variety, Cheonggeum, with Anthracnose-resistant and high yield. This experiment was carried out to select proper pollinizer through researching self-incompatible and Cross-compatibility. Methods and Results : The self-incompatibility test of Cheonggeum was investigated fertilization rate and fruit Characteristics by artificial selfing fertilization. Investigation of fertilization rate and fruit characteristics by artificial fertilization was carried out to test cross-compatibility of Cheonggeum with Cheongwoon, Cheongmeong, Hokwang, Cheonghong and Cheongkwang. Self-fertilization rate of Cheonggeum was 11.1%, self-incompatibility. In flowering period, Cheongkwang, Cheonghong, Cheongmeong, Hokwang and Cheongwoon were early in order. Flowering period of Cheonggeum was June 18. When Cheonggeum was bred as seed parent, the fertilization rate of Cheongmeong and Cheonghong were high. When Cheonggeum was bred as pollen parent, the fertilization rate of Cheonghong and Hokwang were high. Cross-compatibility of Cheonggeum with Cheongkwang or Cheongwoon was low. Anthracnose on fruits in open field was strong in Cheonggeum, Cheongmeong and Cheonghong. The infection rates on leaves to Eriophys macrodonis Keifer was middle in Cheonggeum, Cheongmeong, Hokwang, Cheonghong and Cheongkwang. But it’s was high in Cheongwoon. Conclusion : The proper pollinizer of Cheonggeum in open field was Cheonghong when considering flowering period, cross-compatibility, Anthracnose and Eriophys macrodonis Keifer.

Background : Boxthorn (Lycium chinense M. 2 n = 24) is a self-incompatibility crop of solanales. It can not be fertilized in the cultivation of single cultivars. Therefore it is necessary to select proper pollinizer. We selsected good line, Cheongyang 26, with Anthracnose-resistant and high yield. This experiment was carried out to select proper pollinizer through researching self-incompatible and Cross-compatibility. Methods and Results : The self-incompatibility test of Cheongyang 26 was investigated fertilization rate and fruit Characteristics by artificial selfing fertilization. Investigation of fertilization rate and fruit characteristics by artificial fertilization was carried out to test cross-compatibility of Cheongyang 26 with Cheongwoon, Hokwang, Cheongdang, Cheonghong, Cheongkwang and Cheonggeum. Self-fertilization rate of Cheongyang 26 was 19.3%, self-incompatibility. In flowering period, Cheongkwang, Cheonghong, Hokwang, Cheongyang 26 and Cheongdang were early. The fertilization rate of Cheongkwang, Cheonggeum, Cheongwoon, Hokwang and Cheongdang in order was high when Cheongyang 26 was bred as seed parent. The fertilization rate of Cheonggeum, Hokwang, Cheongwoon and Cheongdang in order was high when Cheongyang 26 was bred as pollen parent. Cross-compatibility of Cheongyang 26 and Cheonghong was very low because of inbreeding. Anthracnose on fruits in open field was strong in Cheongyang 26, Cheonghong and Cheonggeum. The infection rates on leaves to Eriophys macrodonis Keifer was high in Cheongyang 26 and Cheongwoon. Conclusion : The proper pollinizer of Cheongyang 26 in open field was Cheonggeum when considering flowering period, cross-compatibility, Anthracnose and Eriophys macrodonis Keifer. In rain-shelter plastic greenhouse, the proper pollinizer of Cheongyang 26 was Cheongwoon because it’s able to prevent Anthracnose in rain-shelter plastic greenhouse.

Background : Recently, there is an urgent demand for development of new varieties with enhanced resistance to various biotic/abiotic stresses. One generation of ginseng is four years, so it takes a long time to breed. To increase the efficiency of the ginseng breeding and save time and effort, it is necessary to build a ginseng core collection. This study examined the major growth characteristics of genetic sources for the establishment of core collection. Methods and Results : As plant materials, 187 ginseng lines collected in 2003 were used in this study. Ginseng Seeds were harvested at the end of July 2014, sown in mid-November, and cultivated in 2015 for one year in the field and then transplanted into the main field in 2016. All phenotypes including agronomic characteristics, seed yield, and physiological response to biotic/abiotic stresses were investigated according to the ginseng GAP and UPOV guidelines. The stem colors of the collected ginseng germplasm were classified as the five classes; light green, green, light purple, purple and dark purple, but most of them were purple or dark purple. The locations of distribution of anthocyanin coloration in stem were classified into the four classes; proximal end, proximal end and petiole part, the entire stem and the stem with purple not revealed, but most of them were petiole part or the entire stem. The shapes of leaflet were classified the four classes; the long elliptic, elliptic, slender type, and spatulate type. Most of surveyed lines were elliptic type. When the leaflet of ginseng was laterally cut, the shape was classified into the three types; concave type, plane type and convex type. Most of surveyed lines were red berry color. Conclusion : The genetic pool of ginseng is known to be narrow. The results of this study showed similar characteristics among the ginseng fields collected. In the future, we will carry out the survey about quantitative characteristics and correlations of ginseng genetic resources for establishment of Korean ginseng core collection.