The antibacterial effect amino acid-copper(Ⅱ) surfactant on fish pathogens was studied. Fish pathogens of Edwardsiella tarda, Vibrio anguillarum, Aeromonas hydrophila and Streptococcus sp. were selected, cultured in nutrient agar and adjusted at 2 × 10 5∼106 CFU/㎖ in phosphate buffer saline before the addtion of amino acid-copper(Ⅱ) surfactant with different concentrations. All tested pathogens died within 1 hour with 1 ppm of amino acid-copper(Ⅱ) surfactant. In comparison with formalin and ET, amino acid-copper(Ⅱ) surfactnat was more effective in antibacterial capacity.

중랑천 하류부에서 종확산계수를 추정하기 위하여 중량천 월릉교 부근의 구간을 1/20로 축소한 정상 수리모형을 이용하였다. 중랑천의 갈수시의 유량을 고려하여 실험을 실시하였으며, 염료는 Rhodamine B를 사용하였다. 염료의 농도-전도도 곡선을 구하였고, 수리모형에서의 전도도를 측정하여 이를 농도로 확산하였다. 종확산계수를 계산하기 위하여 최대농도와 최대농도의 도달시간 관계를 이용하였다. 수리모형 실험으로 측정된 종확산계수를 기존의 경험식들과 비교하였

유역사면 토양 내에서 지하수의 거동을 모의할 수 있는 TOPMODEL을 이용하여 월별 유출량을 추정하였다. 한국수자원공사에서 운영중인 사연댐 유역(124.5 )을 분석대상유역으로 선정하였으며, 국립지리원에서 작성한 1/5,000 수치지도와 지리정보시스템 도구인 Arc/Info를 이용하여 지형인자 등을 분석하였다. TOPMODEL을 이용한 월별 유출량에 대한 모의결과를 관측치와 비교할 때 재현성이 우수한 것으로 판단되었다. 격자 크기에 따른 영향을 검토하

The lacquer is one of the most important resources for endogenous development and cultural reproduction in Wonju. However traditional and social network, which had been formed by lacquer farmers, group of lacquer-pickers, refineries, and lacquer-ware craftsmen, is being collapsed. It is due to the recent imports of raw lacquer from China and refined lacquer from Japan. Public sectors including Wonju city have continuously improved the potential of lacquer production by planting lacquers. However, the potential of lacquer production isn't under enough condition to realize its value. In this study, it is suggested that the efforts of public sectors to raise lacquer industry, focused on the improvements of lacquer production potential until now, should be concentrated on realization of the production potential by reconstructing social productive system which is composed of diverse groups involved in lacquer ware production.

The relationship between the distribution of sea surface temperature(SST) and dinoflagellate(Cochlodinium polykrikoides) bloom areas were studied. The SST data were derived from the infrared channels of AVHRR(Advanced Very High Resolution Radiometer) sensor on NOAA(National Oceanic and Atmospheric Administration) 12 and 14 satellites during 1995-1998. The initial water temperature at C. polykrikoides bloom was about 21℃ at the coastal areas of the South Sea and along the shore of the East Sea of Korea during the summer season of 1995. The northern limit of red tides was coincident with that of 21℃ isothermal line in the East Sea. The red tides that initially bloomed at the coast of Pohang on September 21, 1995 moved to the coast of Uljin on September 26, 1995. The skipped appearance of the red tides in the areas between Pohang and Uljin was due to the East Korean Warm Current, which was moving offshore from Pohang to approach to Uljin. The cold water which was formed by tidal front in the western coast of the South Sea and by upwelling water from deep layer in the southeastern coast of the Korean peninsula played a role in blocking the spreading of red tides during summer season in 1997 and 1998. In conclusion, the distribution of red tides appeared to be dependent on the initial water temperature at red tides bloom. The SST at the red tides varied from 21℃ to 25℃ ; 21℃, 23℃, 24℃ and 24-25℃ in 1995, 1996, 1997 and 1998, respectively.

This study is to develop the technology and the ways of the practical use of the integrated digital map of and Electronical Navigational Chart(ENC) and Digital Terrain Map(DTM) for the effective and scientific based conservation, development and management. In this study, as preliminary studies to make eventual integrated maps, the concept of coastal areas are defined and the characteristics of digital maps developed by Korean Geography Institute and National Marine Investigation Institute are carefully analyzed. A test coastal map was developed based on the integrated digital map, a high resolution satellite image and Global Positioning System. Results from the edge matching analysis of coastal lines shows 8 meters difference in maximum. The problems, causes and solutions for the edge matched differences are described. Furthermore, the practical value of utilization, future use and various field of application are described based on the integrated digital map data base of coastal area.

무경운 담수표면산파재배시 잡초성벼의 발생양상과 비선택성 및 토양처리제를 이용한 잡초성벼 방제 결과는 아래와 같다. 1. 무경운에 의해 토양표면에서 월동한 잡초성벼의 생존율은 92.7%였고 재배벼는 4.3%였다. 2.토양내의 잡초성벼 발생심도는 건답에서 6.8cm, 무논에서 3.0cm, 그리고 무경운답에서 1.5cm로, 무경운답의 잡초성벼 발생은 건답과 무논에 비하여 토양 표층부위에서 주로 발생하였다. 3. 4월 하순의 우리나라 중부지역 평균기온인 13℃ 에서 잡초성벼 발아에 소요되는 일수는 14-15일이었다. 4. 무경운상태에서 포장 표면위에 떨어져 있는 잡초성벼의 발아를 유도한 후 비선택성 제초제인 paraquat 처리한 결과 92.2%의 잡초성벼를 방제할 수 있었으며, 토양처리제 중 가장 높은 출아억제를 보인 제초제는 oxadiazon으로 53.3%의 방제 효과가 있었다. 5. Paraquat과 oxadiazon의 체계처리에 의해 96.4%의 잡초성벼를 방제할 수 있었다.

Yields of various solvent extracts for Zanthoxylum schinifolium and Z. piperitum leaves were higher in ethanol extract layer. Ethanol Extract in sancho and chopi leaves was fractionated with different solvents, such as hexane, chloroform, ethyl acetate, butanol and water, yields of hexane fraction was higher. In the solvent extracts using methanol, ethanol, hexane, chloroform, and water, ethanol extract showed the most effective antimicrobial activities. Ethanol extract in sancho and chopi leaves was fractionated, the most antimicrobial activities of sancho leaf were ethyl acetate fraction, and chopi leaf were chloroform fraction.

To study the potential of the Zanthoxylum schinifolium and Z. piperitum leaves, as raw materials for functional food and medicine, apart from male and female, chemical components were carried out. Among general components of sancho and chopi leaves, moisture and crude protein were higher sancho leaf than chopi leaf, but total sugar and crude fat were higher chopi leaf than sancho leaf and the components of major minerals were K, Ca, Mg and Na. Among free sugars, glucose(0.24% and 0.21%) and sucrose(0.19% and 0.27%) were the highest contents in sancho and chopi leaves(male and female), respectively. The organic acid were isolation and identification as malic acid and citric acid, citric acid is higher than malic acid. The total amino acid of sancho and chopi leaves contained proline and glutamic acid in male and female sancho and female chopi leaves, glutamic acid and aspartic acid in male chopi leaf highly in order. The fatty acid contents of four samples were high 15.16%, 9.76%, 8.78% and 9.29% of linolenic acid, respectively. Among many volatile compounds, limonene(13.25% and 19.16%) and citronellal(34.37% and 29.66%) were predominant flavor compounds in sancho and chopi leaves(male and female), respectively.

Today freeway is experiencing a severe congestion with incoming or outgoing traffic through freeway ramps during the peak periods. Thus, the purpose of this study is to identify the traffic characteristics, analyze the relationships between the traffic characteristics and finally construct the delay predictive models on the rap junctions of freeway with 70mph speed limit. From the traffic analyses, and model construction and verification for delay prediction on the ramp junctions of freeway, the following results were obtained : ⅰ) Traffic flow showed a big difference depending on the time periods. Especially, more traffic flows were concentrated on the freeway junctions in the morning peak period. ⅱ) The occupancy also showed a big difference depending on the time periods, and the downstream occupancy(Od) was especially shown to have a higher explanatory power for the delay predictive model construction on the ramp junctions of freeway. ⅲ) The delay-occupancy curve showed a remarkable shift based on the occupancies observed : Od/〈9% and Od/≥9%. Especially, volume and occupancy were shown to be highly explanatory for delay prediction on the ramp junctions of freeway under Od/≥9%, but lowly for delay prediction on the ramp junctions of freeway under Od/〈9%. Rather, the driver characteristics or transportation conditions around the freeway were thought to be a little higher explanatory for the delay prediction under Od/〈9%. ⅳ) Integrated delay predictive models showed a higher explanatory power in the morning peak period, but a lower explanatory power in the non-peak periods.

본 연구에서는 조기 난소 부전증 환자를 대상으로 난포 자극 호르몬 수용체 유전자의 돌연변이와 발현 양상을 분석하였다. 돌연변이 분석을 위해 환자의 말초혈액에서 genomic DNA를 분리하고 nucleotide 566을 포함하고 있는 exon 7에 특이적인 primer쌍을 이용하여 중합효소 연쇄 반응을 시행하였다. 전기 영동으로 반응산물을 확인한 다음, 돌연변이 여부를 조사하기 위하여 제한효소 절단분석 (Restriction Fragment Length