We used three-dimensional Matrigel culture system to examine the morphognesis of normal and malignant salivary glands cell in vitro including acinar cells(AC), myoepithelial cell(MC), salivary gland adenocarcinoma cells(SGT), mucoepidermoid carcinoma cells(MEC), and immortalized human salivary gland cells(HSG). For this purpose, normal and salivary gland tumor cells cultured in 3-D Matrigel, and characterized histologically and immunohistochemically, compared with same cells grown on monolayer culture and patient tissue from biopsy. 1. In three-dimensional Matrigel culture, HSG cells form acinar structure, SGT cells shows duct like structure, and other AC, MC and MEC cells dont' form any structure , and their morphology was different from that of monolayer cells. 2. Matrigel involved cell proliferation at a similar pattern to cells on plastic monolayer cell cultures, and monolayer cell revealed higher cell viability than that of Matrigel cultured cells. 3. All salivary glands cells on Matrigel or monolayer showed strong PCNA expression, and there is no expression difference in these cells. But some cells including myoepithelial cells in normal and salivary gland tumor tissue showing PCNA lavelling, so there is PCNA expression difference among normal and tumor tissue cells. 4. Actin expression was noted in AC cells on Matrigel, were rare expressed in the other cells except in MEC cells, and was present in myoepithelial cell and ductal cells of normal gland tissue. There is actin expression difference between tissue and cultured cells . 5. S-100 immunoreaction was moderateively positive in MC cells of monolayer culture, myoepithelial cells of normal tissue and pleomorphic adenoma, all cancer cells of mucoepidermoid carcinoma tissue, but significantly decreased in all salivary cells on Matrigel. 6. TGase 2 expression was prominent in MC cells of monolayer and Matrigel cultured, in myoepithelial cells of normal gland and pleomorphic adenoma, epidermoid cells of mucoepidermopid carcioma, and strong reaction in MEC and AC cells of monolayer and Matrigel cultured. 7. Expression of CK in monolayer culture showed strong reaction to CK6 in all sailvary gland cells, and mild reaction to CK10 and CK16 for all salivary cells, CK16 and CK19 expression in monolayer culture was similar to that of Matrigel culture. 8. CK6 and CK10 expression was strongest in AC and MC cells on Matrigel, and CK 4 was negative reaction in AC, SGT, MEC cells, strong reaction in MC cells but mild in SGT cells on Matrigel. Expression of CK was rare in HSG cells compared with other salivary gland cells, CK16 was prominent in SGT cells, CK10 and CK16 showed strongest expression in MEC cells of Matrigel. 9. Monolayer culture of HSG cell shwoing strong reaction to CK6, moderate to CK19 and mild to the others CK, but 3D cultured HSG cells reveal mild expression to CK16, and rare to others CK, intercallated duct in normal gland tissue showing strong to CK19, and mild to the others Ck, so there are CK expression difference in tissue, monolayer and 3-D cultured cells. 10. Monolayer culture of MEC cells represent strong reaction to CK6, mild to other CK, 3-D cells showing increased CK expression including CK6, epidermoid cells and intermediate cells in mucoepidermoid carcinoma tissue reveal positive to CK6 and CK16, mucous cell positive to CK10 and CK19, so Matrigel showed similar CK pattern compared to mucoepidermoid carcinoma tissue rather than monolThese data indicate that the interaction of salivary gland cells with basement membrane is an important factor in salivary gland development and cytodifferentiation, so this model system will be useful to study acinar or ductal differentiation in vitro.ayer cultred.

This study examines the relationship between multiple sales force management practices and performance within each of Miles & Snowꡑs strategy types(1978) and Walker & Ruekertꡑ s strategy types(1987). The findings are as follows : First, Prospectors seem to be associated with increased performance when they are utilizing a relationship selling strategy, internal sales force, moderate levels of supervision, outcome-based control system and incentive-oriented compensation system. Second, Analyzers seem to be associated with increased performance when they are utilizing a relationship selling strategy, internal sales force, outcome-based control system to deal with the instability in their strategic focus. Third, Low Cost Defender seem to be associated with increased performance when they are utilizing a relationship selling strategy, external sales force, low levels of supervision, outcome-based control system and salary-oriented compensation system. Fourth, Differentiated Defenders seem to be associated with increased performance when they are utilizing a relationship selling strategy, high levels of supervision, balanced(outcome+behavior) control system and salary-oriented compensation system.

국내 프로스포츠 산업이 침체기에 허덕이고 있다. 프로축구(K-리그)의 경우 게임당 평균 유료관중이 채 1만명이 안되는 실정이다. 프로야구 또한 마찬가지이다. 국민들에게 승부의 짜릿한 재미와 여가 생활을 즐기게 하는 프로스포츠 산업이 이젠 외면받기 시작한 것이다. 이에 본 연구에서는 프로스포츠 산업이 스포츠 마케팅만의 관점이 아닌 TQM의 개념과 방법론들을 적용하여 품질의 관점에서 원인을 분석하여 프로스포츠 산업이 관중(고객)에게 경기 서비스를 제공하고 있는 서비스 품질을 향상시킴으로써 고객만족을 통해 시장이 활성화 할 수 있는 방안을 마련하고자 한다.

The employees in information system organization are highly exposed to stress. However, there is little theoretical or empirical study on the effects of job stress on IS organizations. A major reason is because few researchers consider consequences of job

전통 사적공간의 효율적인 관리와 보존방안을 위하여 경기도 구리시에 위치한 조선 왕릉의 대표적인 능인 동구릉을 조사하였다. 조사된 식생을 군락으로 구분해보면 졸참나무-신갈나무군락 (Quercus serrata-Quercus mon-golica commnunity), 오리나무군락 (Alnus japonica community), 소나무군락 (Pinus densiflora community)으로 구분할 수 있다. 졸참나무-신갈나무군락의 하위군락으로는 아까시나

교량의 내진성능확보를 위한 유지보수계획을 수립하는데 있어서, 전체 생애주기비용의 최소화를 통하여 최적의 검사 및 수리회수의 결정방법을 제시한다. 생애주기 비용에는 초기비용, 파괴비용 뿐만 아니라, 검사 및 보수비용이 포함된다. 시간에 따른 내진성능저하모델을 손상함수를 이용하여 표현하였으며, 손상감지정도에 따른 Event Tree Analysis를 통하여 유지보수에 따른 파괴확률을 랜덤진동이론을 이용하여 산출한다. 예제로서 10경간 연속교의 최적유지보수방법을 살펴본 결과, 가속도계수가 증가하고, 연약지반일수록 최적유지보수회수는 증가하는 것으로 나타났다.

최근 우리 나라에서는 국립공원의 지정 목적을 효과적으로 달성하기 위한 정책들이 등장하고 있다. 가슴반달곰 보호를 위한 등산로 폐쇄, 병원균 확산을 막기 위한 애완동물 출입금지, 화재를 예방하기 위한 화기 반입 금지 등이 그 예이다. 이러한 정책들은 다소간 국립공원 방문객과 지역주민들에게 불편을 야기할 수 있으므로 그로 인한 저항과 반발을 줄이고 정책의 원활한 추진을 위해 대중 참여를 통한 의사결정의 중요성이 커지고 있다. 본 연구에서는 생태계 관리 정책을 수립하는 과정에서 흔히 발생하는 선호와 가치 사이의 갈등 문제를 국립공원을 사례로 설문 조사를 통해 분석하였다. 242명의 대학생이 참여한 본 연구의 결과, 응답자들의 절대다수(93.%)가 생태계 관리 정책을 수립하는 과정에 대중이 참여하는 것이 필요하다고 응답하였으며, 그 이유로는 대중이나 지역주민이 원하는 바를 정책에 적절히 반영할 수 있다는 점을 꼽았다(51 7%). 또한 생물종보존을 위해 개인의 재산권이 제한될 수 있지만. 그에 대한 보상이 이루어져야 한다고 응답하였다. 가슴반달곰에 대한 개인적 선호를 넘어서 절대 다수의 응답자(97.1%)가 반달곰 보호를 위한 등산로 폐쇄 정책을 지지하는 것으로 나타났다. 국립공원 내 식물 채취에 대해서는 과거부터 해 오던 일이라면 주민들의 식물 채취는 허가해야 한다는 의견이 많았다(53.3%). 본 연구의 결과는 국립공원 생태계 관리 정책 수립 과정에서 대중의 참여를 포함시킬 때 개인적 선호를 넘어 집단적 가치의 측면에서 판단하도록 유도하고. 대중에게 충분한 정보와 토론할 기회를 제공하는 것이 중요하다는 선행 연구의 결론을 지지하는 것으로 생각된다.

본 연구는 남한강 상류 평창군 일대의 하천을 대상으로 1999년 10월-2000년 8월까지 4차례에 걸쳐 Braun-Blanquet의 방법으로 수변식생의 구조를 파악하고자 수행되었다. 또한 현장에서 채취한 토양을 분석하여 하천주변의 식생과 토양과의 관계를 분석하였다. 평창군 일대의 하천에서 출현한 식물은 46과 128속 144종 26변종으로 총 170종이 출현하였으며, 대표적인 하천식생 군락으로 갯버들군락, 달뿌리풀군락, 고마리군락, 뺑쑥군락이 분포하고

This study was conducted to investigate dietary patterns and assimilation of dietary culture of Korean descendants in Yanbian, China compared to Korean. A dietary survey was conducted using one 24-hour recall method from 730 adults over 30 years of age in Yanbian, China and 695 adults over 25 years of age in Kuri, Korea. The average number of food items consumed per day was 14 in Yanbian and 20 in Kuri and there was a significant difference between the two regions(p<0.001). The foods consumed most frequently were rice, salt, the M.S.G. in Yanbian and rice, green onion and garlic in Kuri. The foods consumed most by amount were rice, cucumber, Soju in Yanbian and rice, Kimchi and grape in Kuri. The average number of dishes consumed per day was 6.4 in Yanbian, 9.4 in Kuri and the difference between the two regions was significant(p<0.001). Total number of dishes appeared in the survey was 253 in Yanbian and 494 in Kuri. The dishes consumed most frequently were cooked-rice, beverages, Kimchi, stir-fried eggplant in Yanbian and cooked-rice, Kimchi, coffee, soybean paste stew, in Kuri. The dish groups appeared most frequently were rice, stir-fried foods, vegetables and Kimchi in Yanbian and rice, Kimchi, beverages and fruits in Kuri. Male subjects in Yanbian Consumed alcohol frequently while adults in Kuri consumed coffee more frequently than Yanbian. The major meal patterns were rice+side dish, rice+soup, rice+side dish+Kimchi in Yanbian and rice+soup+side dish+Kimchi, rice+side dish+Kimchi, rice+soup+Kimchi in Kuri. Stir-fried foods, which were consumed 46.6% of all the meals, were dominant side dish in Yanbian, but stir-fried food, seasoned vegetables and grilled food appeared aver 10% in Kuri. There results show that variety of diet of Korean-Chinese in Yanbian was lower than Kuri. Korean-Chinese tend to keep traditional Korean dietary patterns of consuming rice as staple but were adapted to Chinese dietary patterns of consuming stir-fried side dishes. Efforts should be directed toward preserving Korean traditional patterns of dietary culture among Korean-Chinese population.

The main constituent of green tea, catechins have been reported to have numberous biological activites including antimutagenic, antibacterial, hypocholesterolemic, antioxidant and antitumor properties. In the present study, we examined the protective effect of catechin on UVB-induced skin damage. Catechin (3 mg/mouse) was topically treated to dosal area of SHK-1 hairless mouse daily for 2 weeks. UVB (100 mJ/㎠) was also treated soon after application of catechin alone or with catechin for 2 weeks. Catechin reduced UVB-induced infiltration of inflammatory cells, fibrosis of cells and collagen-fiber formation. In addition, catechin also prevented UVB-induced DNA fragmentation and apoptosis cell number, but not changed p53 level. Furthermore catechin inhibited UVB-induced cell proliferation. There results showed that catechin have preventive effect against UVB-induced skin damages, and these effects could contribute to the antitumor promotors activity.

본 연구는 자연생태관 조성계획지인 강원도 평창군 용평면 노동리 노동계곡을 대상으로 식생조사를 실시하여 자연생태관 조성에 필요한 기초자료를 제공하고자 수행되었다. 노동계곡의 조사구에서 출현한 관속식물은 총 56과 121속 152종 24변종 2품종이며, 본 조사에서 감소추세종으로 관중과 도깨비부채가, 한국특산종으로 노랑무늬붓꽃이 출현하였다. 식물군락은 자연군락인 신갈나무-당단풍군락과 인공군락인 일본잎갈나무군락, 잣나무군락으로 구분되었으며, 신갈나무-당단풍군

A survey was conducted to assess the food-purchase behavior of 197 Taejon full time housewives 30 to 49 years of age. Food purchase was assessed using a questionnaire and the resulting data were analyzed using SPSS programs. Demographic data revealed that most of the households have three to five family members and that the Engel Indices of the participating households were mostly between 10 and 30%. Noticeable results of the food purchase assessment are as follows. In the grain and grain products items, the low-education group purchased more rice and barley than the high-education group, whereas the high-education group purchased more glutinous rice, brown rice, bread, macaronis and spaghetti than the low-education group. In the vegetable items, the low-education group purchased more Chinese cabbage and radish than the high-education group, while the high-education group purchased more head lettuce, broccoli and sweet pepper than the low-education group. In the meat items, the beef purchase was higher in the high-education than in the low-education group, while the pork purchase was higher in the low-education than in the high-education group . In the processed meat items, ham was the most favorite purchase item regardless of the education level. In the fish and shellfish items, Pacific cod and Alaska pollack were purchased more in the low-education than in the high-education group, and salmon and dried icefish strip were purchased more in the high-education than in the low-education group. No items in fruit group showed significant differences in the purchase amount between the low-education and the high-education group although the latter purchased more imported-fruits such as melons, kiwis, grapefruits, and oranges. Dairy products such as milk, cheese and butter were purchased more in the high-education than in the low-education group. It was also found that both the number of food items and the consumption of foods coming from animals were higher in the high-education than in the low-education group.

본 연구는 계룡산국립공원 내의 군산보호구역의 산림식생을 대상으로 식물사회확적 방법으로 산림군락을 분류하고, 각 군락과 입지환경과의 상관관계를 분석하여 계룡산국립공원에 필요한 기초자료를 제공하고자 조사하였다. 계룡산 군산보호구역 내 총 94개 조사구를 분석한 결과, 식물군락은 졸참나무군락(Quercus serra-ta community)과 신갈나무군락(Quercus mongolica community)으로 구분되었다. 졸참나무군락은 해발고가 낮고 경사가 완만한 지역에, 신갈나무군락은 해발고가 높고 경사가 급한 지역에 분포하였다. 졸참나무군락은 하위군락인 때죽나무아군락(Styrax japonica subcommunity)과 소나무아군락(Pinus densiflora subcom-munity)으로 구분되었으며, 신갈나무군락은 하위군락으로 전형아군락(typical subcommunity)과 철쭉꽃아군락(Rhodoendron schlippenbachii scbcommunity)으로 구분되었다.