As diethylnitrosamine (DEN) effect on cell proliferation, DNA damage and stem cell marker(s) expression have been largely unknown in mouse normal hepatocytes (AML-12 cells) cultured over a short-term period, this study was conducted to examine the cell proliferation, Ataxia telangiectasia mutated (ATM) and epithelial cell adhesion molecule (EpCAM) and Neighbor of Punc E 11 (Nope) expression in AML-12 cells treated with DEN for 24 and 48 h. Cells were treated with DEN (25-800 μg/mL) and cell phenotype was determined, and the MTT assay was used to quantify the proliferation of cells treated with DEN. Expression and distribution of ATM in AML-12 cells were determined by indirect immunofluorescence microscopy. And Western blot analysis of EpCAM and Nope was performed. Cell viability was significantly increased in response to all doses of DEN treatment compared to control at 24 h (p<0.05 or p<0.01). However, there was no significant increase at 48 h, even though it showed increased trend. Immunofluorescence staining of ATM showed that there was an increase of ATM expression at doses of 50, 100 and 200 μg/mL of DEN treatment, showing strong nuclear staining. Furthermore, Western blot analysis showed that DEN treatment showed increased trend of EpCAM and Nope expression. Taken together, DEN treatment increased cell proliferation in AML- 12 cells, and it was associated with increased ATM expression.

While many scholars paid attention to L2 learner beliefs about grammar learning and error correction, there has been little research on both teacher and learner beliefs about types of grammar instructions in Korean EFL contexts. This study aimed to investigate the beliefs about types of instructions as well as grammar learning and error correction using a questionnaire adapted from Jean and Simard (2011). A total of four hundred and eighty-seven participants in Korea participated in the study and were divided into five groups: three teacher subgroups (non-native Korean high school, non-native Korean college, and native college) and two student subgroups (high school and college). The participants in general highly valued accuracy in L2 learning, but they disliked grammar learning or teaching. While both students and teachers believed that grammar instructions such as discovery learning, and mechanical practice could be useful in grammar learning, Korean teachers reported more positive views on grammar instructions than Korean students. The majority of the participants reported that they had a tendency to prefer error correction in the written production rather than in the spoken one. The results also revealed opinion gaps among the subgroups. The pedagogical and theoretical implications will be discussed.

This study aimed to investigate antioxidant activity of various extracts from fruiting bodies and mycelia of two Phellinus linteus strains and P. baumii. The Phellinus strains have cultivated on oak and mulberry logs. The fruiting bodies species were harvested from each Phellinus strain and used in this study. The tested items include: 2, 2’-azinobis[3-ethylbenzthiazoline]-6-sulfonic acid (ABTS), free radical scavenging assay and determination of total phenolics contents (TPC), ferric reducing antioxidant power assay (FRAP), and DPPH (1, 1-diphenyl-2- picrylhydrazyl) radical-scavenging activities. Different extractions with 60% Ethyl alcohol, 70% methyl alcohol and heat water were done on the mycellial and fruiting bodies samples of the mushroom species. The methyl alcohol extraction from fruiting body of P. linteus KACC93057P displayed the highest antioxidant activity on ABTS, FRAP, and DPPH assays. The ethyl acetate fraction was concentrated and subjected to an ODS column chromatography, followed by Sephadex LH-20 column chromatography. Finally six compounds 1-6 were detected by preparative reversed-phase HPLC.

To determine the medicinal properties of Pleurotus cornucopiae and Safflower Oil (PS), the osteoblast effect was investigated. PS can prevent estrogen deficiency-induced bone loss. It was taken the measurements of biochemical factors such as serum osteocalcin, alkaline phosphatase, IGF-1, and bone status. Forty adult female Sprague-Dawley rats were randomly assigned to four equal groups for 12 weeks of treatment: (1) sham operation (SHAM) + vehicle; (2) ovariectomy (OVX) + vehicle; (3) OVX + 17β-estradiol (25 μg/kg); (4) OVX + PS (300 mg/kg/d, PO). At the end of the experiment, bone turnover, and trabecular microarchitecture were assessed by micro-computed tomography (micro-CT), and histopathology, respectively. Ovariectomized rats experienced remarkable increases in global volumetric bone density, and trabecular microarchitecture deterioration. These OVX-induced pathological changes are reversible in that most of them could be mostly corrected upon 17β-estradiol treatment. PNS treatment significantly prevented bone mass loss and microarchitecture deterioration. Moreover, PS enhanced osteoblast activity but suppressed osteoclast turnover, as evidenced by decreased levels of serum C-terminal telopeptides of type I collagen and elevated levels of alkaline phosphatase. PS mitigates estrogen deficiency-induced deterioration of trabecular microarchitecture.

Two dimensional finite element method with quadrilateral basis functions was applied to the spherical high order filter on the spherical surface limited area domain. The basis function consists of four shape functions which are defined on separate four grid boxes sharing the same gridpoint. With the basis functions, the first order derivative was expressed as an algebraic equation associated with nine point stencil. As the theory depicts, the convergence rate of the error for the spherical Laplacian operator was found to be fourth order, while it was the second order for the spherical Laplacian operator. The accuracy of the new high order filter was shown to be almost the same as those of Fourier finite element high order filter. The two-dimension finite element high order filter was incorporated in the weather research and forecasting (WRF) model as a hyper viscosity. The effect of the high order filter was compared with the built-in viscosity scheme of the WRF model. It was revealed that the high order filter performed better than the built in viscosity scheme did in providing a sharper cutoff of small scale disturbances without affecting the large scale field. Simulation of the tropical cyclone track and intensity with the high order filter showed a forecast performance comparable to the built in viscosity scheme. However, the predicted amount and spatial distribution of the rainfall for the simulation with the high order filter was closer to the observed values than the case of built in viscosity scheme.

Insecticidal susceptibility of Western flower thrips, Frankliniella occidentalis, was investigated under laboratory and greenhouse conditions using commercial insecticides at their recommended concentrations. Neonicotinoid insecticides, especially acetamiprid SP, clothianidin SC, imidacloprid WP and thiacloprid WG were not toxic to F. occidentalis collected from horticultural crops. Excepting the insecticide of spinosin or pyrrole class, there was a little class of insecticides for the effective control of F. occidentalis in horticultural crop areas. Considering all the results in this study, spinosin insecticides including over 4 and 10% of spinetoram and spinosad, and pyrrole insecticide including over 5% of chlorfenapyr should be recommended for the effective control of F. occidentalis

The aim of this paper is to illuminate some aspects of the Jeju 4.3 Grand Tragedy (the Jeju Tragedy) education and informing process. We hope to not only with these aspects show some aspects of moral origin of the Jeju World Peace Island movement, but to share a culture of peace with world citizens through world civilization education cross the Pacific at a local, national and global level. In addition bring liberty to the cause of national freedom of the SAM IL movement of 1919 in Korea. it actualizes itself as a peace buffer zone locally, nationally and globally through the “Jeju World Peace Island Treaty JWPIT,” based on international agreement of the Six Talks Nations because “it is believed that that they (Jeju islanders) will follow the pattern of the SAM IL movement of 1919 with the participants imbued with the spirit of sacrifice to life and liberty to the cause of national freedom (G-2 Summary Report)” as a moral origin of Jeju World Peace Island. Globally, one New Haven teacher has been working on an elective course called “The Power of Culture in a Multicultural World” in which to use social studies tools to investigate the meaning and importance of culture. Jeju offers an excellent opportunity to explore a culture that few New Haven students are familiar with. It will be for students to use the Jeju idea of turning troubles into opportunities, as a vehicle for addressing local issues” (“New Haven Learn- Teach Jeju 4.3,” New Haven Independent: March 29, 2017). Another New Haven teacher in his history class explains the different components of genocide. “Although the Jeju 4.3 events are not deemed genocide by the international community, using this as a lens to understand broader concepts seemed really exciting.” Jeju offers educators an opportunity to teach the curriculum but instead of gratuitous death and violence, there is a legacy of hope, healing, reconciliation and remembrance.” (Ibid, New Haven Independent: March 29, 2017). Locally and partially on the global level, “The Resurgence of Jeju 4.3 Social Healing Advocacy of “PETITION FOR A JOINT SOUTH KOREA AND UNITED STATES JEJU 4.3 INCIDENT TASK FORCE TO FURTHER IMPLEMENT RECOMMENDATIONS AND FOSTER COMPREHENSIVE AND ENDURING SOCIAL HEALING THROUGH JUSTICE” (March 27, 2014). The petition supports a combined solution to social healing for numerous survivors of Jeju 4.3. Based on interviews with members of the Jeju 4.3 Victim Family Association, 20% of them are now elderly and require expensive medical care and assistance. Many continue to suffer from psychological trauma (PTSD). It serves as a beacon of international peace in the same vein as May 24, 2015’s event, “Women Cross DMZ Demilitarized Zone.” Jeju itself aims to serve as a “Peace Buffer Zone” between the two Koreas among 6 Nations Talks within the framework of the Jeju World Peace Island Treaty, similar to Costa Rica. Never before has the need for a Peace Island in the region been so vital, with China stretching its reach across the ocean, and President Trump flexing his muscles against North Korea, the time has come for serious peacemaking, that is, prevention of conflict. It’s a real time to think about actualization of Jeju World Peace Island Treaty for Peace between two Koreas.

This study aims to acquire basic data for creating a new landscape forest by examining and analyzing both current and desired images of a forest in a rural neighborhood park. Regarding the forest in Janggye Neighborhood Park, located in Janggye-myeon, Jangsu-gun, Jeollabuk-do Province, this study conducted a questionnaire, using one-on-one interviews, to survey residents in Janggye-myeon who visited the park, and a total of 58 questionnaire copies were used for analysis. The current image about the forest was analyzed as giving a common and non-mysterious impression, and the subjects did not notice seasonal changes very much. Overall, the residents had negative images about the current forest. The desired image of the forest is a forest that is amiable, beautiful, comfortable, safe, and pleasant. A t-test was conducted to identify differences between the current image and the desire image, and its results showed that there were significant differences in most variables. The factor group implying the forest image consists of fascination dimension and diversity dimension. When factor scores of the current image and the desired image were compared, there were bigger differences in the fascination dimension scores than in the diversity dimension scores. As a result, it is drew that it would be much more effective to prioritize images related to fascination dimension in creating a forest landscape in Janggye Neighborhood Park.

The purpose of this study is two-fold: (1) to find context-specific motivational factors in Korean college contexts within the framework of Gardner’s integrativeness and Dörnyei’s (2005, 2009) L2 Motivational Self System; (2) to examine how those motivational factors explain motivated behavior. For this purpose, motivation data by 787 students was analyzed. The factor analysis revealed eight factors in Korean context: promotional instrumentality, bad learning experience, ought-to L2 self, integrativeness, elusive ideal L2 self, ethnocentrism, intercultural avoidance, and international posture. The regression analysis showed that four motivational factors, bad learning experience, ought-to L2 self, elusive ideal L2 self, and promotional instrumentality, significantly explained the participants’ motivated behavior. The findings indicate that there existed context-specific motivational factors which could better explain success of L2 in Korean EFL college contexts. The theoretical and pedagogical implications were provided at the conclusion.

Free radicals originate due to the radiolysis of cytoplasmic water with low “Linear Energy Transfer” (LET) radiations. Naringenin (Ng) is a natural antioxidative compound found in citrus fruits. This study revealed that Naringenin (Ng) reduced the radiation damage of critical organs by scavenging oxidative free radicals. In the study, Ng was orally administrated to rats daily for 7 consecutive days, prior to whole body exposure to gamma-rays. The scavenging efficacy was evaluated biochemically by measuring the concentration of cytotoxic byproducts and the activity of enzymes relevant to oxidative free radicals, after extracting the organs from the exposed rat. We observed increased levels of malondialdehyde (MDA) concentration, and decrease in the activities of superoxide dismutase (SOD) and catalase (CAT) in the exposed control group. However, pretreatment with Ng significantly reduced the MDA concentration, and increased the activities of SOD and CAT, as compared to the control group, due to the free radical scavenging by Ng. The results indicate that Ng administration prior to irradiation could protect critical organs from radiation damage.

In recent years, the tourism experience of tourists has been impacted seriously with the increase in the number of tourists. Psychological Carrying Capacity (PCC) as an important part of Tourism Carrying Capacity (TCC), is an important criterion for measuring the quality of tourism experience. It is also an important and necessary means of tourism management. Daerungwon, as a symbol of the Gyeongju city, has not been studied on TCC. In this study, the number and the satisfaction degree of tourists in Daerungwon were investigated hourly. Then the mathematical relationship between the two variables was established with regression analysis method. Based on these, the study researched the change rules of the PCC in Daerungwon. The results showed that: (1) The satisfaction degree curve presented inverted “U” shape rather than monotonically decreasing shape with the increase of the number of tourists in Daerungwon. (2) When the instantaneous number of tourists is 193, the satisfaction degree of tourists is the highest. When the instantaneous number is 382, the descent speed of satisfaction degree is the fastest. The best PCC in Daerungwon is 1,198 persons per day. And when the number of tourists reached 2,372, the satisfaction degree will drop quickly. Finally the study verified the influencing factors of PCC from the view of tourists and put forward suggestions on management based on PCC research results. The achieved results can provide the references to the tourism management of Daerungwon.

Hematometra is defined as the accumulation of blood and blood clots in the uterus. An 8-month-old, intact female Poongsan dog presented with continuous severe hemorrhagic vulvar discharge and anemia. The initial diagnostic evaluation included abdominal ultrasonography, radiography, and physical and laboratory examinations. The electrolyte levels were low: Na ion levels were 128 mmol/L (reference range, 141 - 152 mmol/L), and Cl ion levels were 99 mmol/ L (reference range, 105 - 115 mmol/L). At presentation, the white blood cell count was 60.19 × 103/μL (reference range, 5.05 – 16.76 × 103/μL), packed cell volume was 11.8% (reference range, 37.3% - 61.7%), hemoglobin levels were 4.6 g/ dl (reference range, 13.1 - 20.5 g/dl), and platelet count was 48 × 103/μL (reference range, 148 - 484 × 103/μL). Based on the results of the complete blood count, the dog was given lactated Ringer’s solution and a whole blood transfusion. On abdominal ultrasonography, the left uterine horn was enlarged and filled with echogenic fluid. On laparotomy, the uterus was enlarged and showed an accumulation of blood and blood clots. The incised uterine horn revealed that the endometrium was filled with blood and blood clots and had a cystic appearance. Additionally, thick and yellowish pus had accumulated in the uterine cavity. Gross findings revealed a definitive diagnosis of cystic endometrial hyperplasia- pyometra complex with severe hemorrhage, termed hematometra. This case was the first report of severe hematometra in a young Poongsan dog.

This report describes the different responses to dapsone treatment in two cases of sterile nodular panniculitis (SNP). Two dogs were presented with ulcerative skin lesions, painful and erythematous papules, and nodules. History and physical examination revealed systemic signs such as pyrexia, lethargy, depression, and anorexia, in addition to ulcerated and ruptured nodules on the skin. The dermatological diagnostics included clear taping, trichogram, skin scraping, impression smears, fungal and bacterial cultures, and histopathology and special stainings of multiple punch biopsies obtained from the skin lesions. Based on the clinical and histopathologic findings, the absence of microbiological infection, and the positive response to immunosuppressive therapy, both the dogs were diagnosed with SNP. Although both dogs had been treated with various immunosuppressive drugs including prednisolone, cyclosporine, azathioprine, and triamcinolone, therapy was switched to dapsone due to recurrent dermatological signs and presumed steroidinduced hepatotoxicity. The clinical responses to dapsone were opposite in the two cases. In the first case, combination therapy with prednisolone and cyclosporine was effective in attenuating ulcerative lesions, while dapsone alone did not control the clinical signs. In contrast, in the second case, the therapeutic response to the common immunomodulatory drugs such as prednisolone, triamcinolone, and azathioprine was inadequate. Interestingly, dapsone alone was effective in controlling the clinical signs without causing undue side effects. Although the usefulness of dapsone for the treatment of canine SNP is unknown, it may be considered in mild to moderate cases of SNP when the use of steroids is not recommended due to its low efficacy or side effects.

One of the important advantages of Thermosonics is that it can be applied to complex structures such as a turbine blade as a convenient and quick screening test method. For a reliable thermosonic test, the vibrational characteristics of the system comprising the tested structure and the clamp at ultrasonic frequency range should be identified. Therefore, this study presented the analysis results of frequency response functions and mode shapes of the turbine blade and clamp system and investigate the possibility of the reliable excitation system for the thermosonic test.