Stathmooda masinissa is one of the most serious lepidopteran pests which damaging the furit of persimmon. Its pheromone composition has been idetified as (E4,Z6)-4,6-hexadecadienyl acetate (E4,Z6-16Ac) and (E4,Z6)-4,6-hexadecadien-1-ol (E4,Z6-16OH). We evaluated electrophysiological response of (E4,Z6)-4,6-hexadecadienal (E4,Z6-16Ald) and attractivenss of E4,Z6-16Ald by combination of two pheromone components. E4,Z6-16Ald evolked electrophysiological responses as same to that of two pheromones. The lure combined with E4,Z6-16Ald and two pheromone components attracted more male S. masiissa.

Pine wood nematode (PWN), Bursaphelenchus xylophilus causes devastating damage to pines worldwide. To develop new agent for controlling PWN, alkyloxyalcohols(A-H) were synthesized and nematicidal activity was tested against PWN. As a control, monochamol, which reported to have nematicidal activity was also tested. The nematicidal activity was diffferent according to carbon chain length in compounds. LC50 values of F, G and H were as same as that of monochamol. However A-E which have shorter carbon length than F-H showed weak nematicidal activity.

Pine wilt disease (PWD) induced by the pinewood nematode (PWN), Bursaphelenchus xylophilus, is a great threat to pine forests in Europe and East Asia. Identification of interrelation between Monochamus spp., the major vectors of PWD and PWN is the key factor for effective control of PWD because PWN is trasmitted to healthy pine trees only by Monochamus vectors. In this study, direct injection system is designed to apply PWN artificially to M. alternatus just before emergence to adults. After injection of PWN (300~500 nematodes in 10 μl of 1X PBS) using adapted glass pipets, the survival rate of M. alternatus and detection rate of live PWN were 80.0% and 68.8% at 168 h respectively. As a result, we demonstrated the artificial injection methods of nematode to Monochamus vectors for further study of coeffect between vectors and specific stage of PWN including 3rd stage dispersal juvenile.

전국 136개 시군에 있는 690개소 벼 관찰포에서 12종의 주요 해충을 6월 1일부터 9월 13일까지 15일 간격으로 총 8회 조사한 결과를 국가농작물병해충관리시스템(NCPMS)을 통하여 수집하였다. 2017년도 벼 주요해충 12종의 총 발생 추정면적은 158,942ha로 재배면적 대비 21.1%에 발생되었다. 발생면적이 가장 많았던 해충은 애멸구로 43,875ha(재배면적 대비 5.8%)이였고, 그 다음으로는 혹명나방 34,112ha(재배면적 대비 4.5%), 벼물바구미 22,802ha(재 배면적 대비 3.0%), 먹노린재 21,118ha(재배면적 대비 2.8%)순 이었다. 평년보다 발생면적이 많았던 해충은 애멸구, 멸강나방, 먹노린재로 애멸구는 평년보다 113%수준, 멸강나방은 평년대비 136% 수준, 먹노린재는 269% 수준이었다. 먹노린재의 발생이 점차 증가하고 있는 것을 확인할 수 있었다. 결론적으로 2017년 벼 주요해충의 발생정도는 전년 대비 96% 수준으로 유사하였지만, 평년대비는 56% 수준으로 발생이 낮은 경향임을 확인하였다.

Mediterranean fruit fly, Ceratitis capitata, is one of the most important quarantine pest worldwide. Fruit flies cause serious damage on orchard, and also can cause quarantine problem to fruit exportation. Recently, because of global warming, there is threats of fruit fly invasion to Korea, especially to Jeju island. So there should be a anticipative research about fruit fly control. Many physical quarantine treatment methods, like heat treatment and low-temperature treatment, are developed to control quarantine pests. Recent researches indicate that low-temperature treatment shows good efficacy on several quarantine pests without phytotoxicity. In this trial, we’ve applied the low-temperature treatment on citrus at laboratory and pilot scale containers to validate efficacy and phytotoxicity. Egg and larvae stage of C. capitata was completely controlled after 5 days and 8 days low-temperature treatment at 0℃ respectively, and there was no phytotoxicity on citrus. These results indicate that the low-temperature treatment can be applied to control fruit fly on citrus fruit.

Carbonyl sulfide is a chemical that is registered as a crop protection product in Australia due to microorganisms such as soil, plant roots, branches, fertilizers and compost. In order to develop new fumigants for domestic use, the susceptibility of Carbonyl sulfide fumigants to two agricultural pests was evaluated. The susceptibility of peach aphid and spotted mite, which is an agricultural pest, was evaluated. After 5 hours of treatment (10, 20, 40, 60, and 80 mg / L) . As a result, peach aphid larvae showed a high mortality rate of 97.6% at 10 mg / L and 100% at 20 mg / L or more. Larvae were found to have a rate of 95.8% at 80 mg / L. The insect mortality rate was 91.8% at 60 mg / L and 94.1% at 80 mg / L.

The susceptibility of the Cigarette beetle, which are pests in tobacco, to methyl bromide and phosphine fumigants was evaluated. Five concentrations of each methyl bromide were selected for all stages and treated for 4 hours. As a result, 100% larvae were observed in eggs CT 51.20, late larva CT 73.61, pupa CT 71.87 and adult 52.87 mg h / L, respectively. The LCT50 values for methyl bromide were 13.896 for eggs, 36.038 for late larvae, 25.172 and 21.758 mg / l, respectively. The phosphine treatment was carried out for 5 to 6 concentrations (0.025, 0.051, 0.099, 0.501, 0.999 and 1.500 mg / L) for 20 hours. As a result, egg and late larvae showed 100% larvae at CT 5.137 and 6.435 mg h / L, respectively, and 61.9% and 98.9% of larvae and adult larvae were similar to each other. Pupae showed 86.4% larvae at CT 10.520 and 100% larvae at over 13.777 mg / h of CT. The LCT50 values for phosphine were 0.317 for eggs, 0.649 for late larvae, 3.748 for pupa and 0.703 mg / l for adults.

Riptortus pedestris is a polyphagous species and an economically important insect pest of soybean. We investigated the phenology of R. pedestris at 13 locations - Jeonju, Naju, Goheung, Milyang, Gunwi, Andong and 7 locations of Jeju. Fish trap was used for monitoring R. pedestris. The traps were baited with lures containing a 1:5:1 ratio of myristyl isobutyrate, (E)-2-hexenyl (E)-2 hexenoate and (E)-2-hexenyl (Z)-3-hexenoate. There was difference the first adult catch and local temperatures among sampling sites. To determine spring emergence distribution and annual phenology pattern, we used the monitoring data and ambient temperature from each sampling sites. We compare the seasonal occurrence and spring emergence between 2016 and 2017 in each location.

Riptortus pedestris is one of economically important insect pests in soybean fields. The study was conducted at six constant temperatures of 15.8, 19.7, 24.0, 27.8, 32.6 and 35.5℃. We investigated the biological traits of R. pedestris adults. R. pedestris adults survived at all temperatures tested but female did not oviposit under 15.8℃. The longevity of R. pedestris male was longer than that of female. Oviposition model was constructed using nonlinear functions and the number of eggs produced by a cohort of females was estimated in relation to constant temperature and adult age. The life table parameters in relation to temperature were analyzed.

The oriental fruit fly is a major polyphagous insect pest with a worldwide distribution. The effects of temperature on stage-specific development were investigated at eight constant temperatures (13.0, 14.4, 16.2, 19.5, 23.8, 27.7, 31.8 and 34.8℃). Stage-specific lower developmental thresholds and thermal constants were determined using linear regression. The lower and higher temperature threshold (TL and TH) were estimated using the Sharpe-Schoolfield-Ikemoto (SSI) model. The daily adult emergence frequency of B. dorsalis was estimated in relation to adult age and temperature. Thermal performance was compared among B. dorsalis populations from different locations in Taiwan.

The oriental fruit fly, Bactrocera dorsalis (Hendel), has potential of serious damage to various vegetables or fruits, especially genus of Citrus, such as mandarin orange and tangerine in Korea, where its larvae do damages. Animal and Plant Quarantin Agency of Korea and Animsl Systematics Laboratory of Kunsans National University have collected the samples of oriental fruit fly in East Asian countries, Vietnam, Philippines, Myanmar, China, Cambodia, India Nepal and Laos. To confirm genetic differences and structure of B. dorsalis population samples collected from those countries, we analyzed 192 individuals from 28 locations over 7 countries using 15 microsatellite loci. In total samples, number of different alleles, number of effective alleles and Shannon's Information Index were 6.421±0.364 (standard error, SE), 3.664±0.137 (SE) and 0.902±0.030 (SE), respectively. Grand means (±SE) of observed and expected heterozygosity over all loci and populations were 0.534 (±0.018) and 0.597 (±0.017), respectively. Among all populations, Fst values ranged from 0.016 to 0.705 with averaging 0.194 (±0.026).

본 조사는 채집방법에 따른 곤충군집을 비교하기 위해 공검지 습지보호지역 내에서 쓸어잡기, 함정트랩, 버킷트랩 세 가지 방법을 이용하였다. 조사 시기는 5월 15~16일, 8월 3~4일, 11월 6~7일 총 3회 실시하였으며, 조사는 보호지역 중심부에서 실시하였다. 본 연구결과 세 방법으로 총 7목 51과 110속 120종 14,717개체가 채집되었다. 채집방법별로 쓸어잡기에서 4목 25과 36속 37종 331개체, 함정트랩에서 3목 12과 18속 19종 30개체, 버킷트랩에서 6목, 38과 76속, 84종, 14,121개체가 조사되었다. 군집 분석결과 다양도 지수는 쓸어잡기가 3.63으로 가장 높게 나타났으며 풍부도 지수는 버킷트랩이 8.69로 가장 높게 나타났다. 유사도 지수는 세 방법이 서로 0.2이하로 매우 낮았다.

참나무와 서어나무는 온대지역에 걸쳐 넓게 분포하고 있으며, 다양한 초식곤충의 먹이원이 되는 것으로 알려져 있다. 이 연구에서는 지리산국립공원 온대림에서 참나무림(반선)과 서어나무림(상선암)에서 초식곤충 활동량을 알고자 하였다. 각 조사지역에 0.1ha의 방형구를 설치하여 참나무와 서어나무의 분포를 확인 한 뒤 개체별로 초식곤충의 활동량인 잎 손상 지수를 측정하였다. 잎 손상 지수는 손상 퍼센트로 환산하여 분석하였다. 측정결과 전체 4,413장의 잎을 관찰하였으며, 참나무림에서는 2,683장, 서어나무림에서는 1,730장의 잎이 조사되었다. 초식곤충의 활동량은 참나무림과 서어나무림에서 각각 차이를 보였으며 서어나무림인 상선암에서 참나무의 식흔량과 서어나무의 식흔량 이 참나무림인 반선보다 모두 높게 나타났다. 이 연구결과를 바탕으로 온대림 우점 수종인 참나무와 서어나무에서 초식곤충의 활동량을 정량적으로 추정할 수 있을 것으로 생각된다.

One of the leading pests of rice, small brown planthopper (Laodelphax striatellus) can grow up to have either short or long wings, depending on conditions. However, under the same breeding conditions, the phenotypes of the long- and short-winged small brown planthopper observed to keep the first collected phenotype. To investigate the mechanism involved in wing dimorphism, metabolomic researches have been conducted. In this study, we observed several metabolites change, and the difference of metabolites could provide clues to the relationship between physiological changes in the small brown planthopper and ecological transport.

Mosquitoes are transmit many dangerous disease such as malaria, yellow fever and dengue fever. So far, chemical insecticides such as DEET have been mainly used to control mosquitoes, but there are many side effects. This study used ultrasonic sounds as an alternative to chemical insecticides. We found that Culex pipiens, which are common in Korea, exhibit avoidance behavior in a specific ultrasonic frequency. Through electrophysiological recording, we have inferred that avoidance behavior is caused by different from each other mechanisms depending on the ultrasonic frequency. Using immunohistochemical staining, we analyzed the expression pattern of auditory related genes in the chordotonal organ. Quantitative real time-PCR was used to compare the expression levels of auditory related gene depending on the time of exposure to ultrasonic sounds.

감귤그린병(Citrus Huanglongbing Disease)은 전 세계적으로 감귤산업에 가장 큰 피해를 주는 병해로 국내 미분포종인 귤나무이(Diaphorina citri Kuwayama)가 매개충으로 알려져 있다. 바이러스에 감염된 나무는 5~12년내에 대부분 고사하며 열매는 크지 못하고 색이 들지 않는 증상을 보여 상품성을 저해시킨다. 현재 접목, 묘목, 매개충등의 유입으로 전 세계적으로 확산되고 있는 실정이다. 귤나무이의 국내 분포가능성이 꾸준히 제시되면서 유입 대비를 위한 예찰방법 및 동정법 개발이 필요한 실정이다. 제주도에는 총 33종이 분포하는 것으로 알려져 있으며(권, 1983; Cho & burckhardt, 2017) 2016년부터 지금까지의 조사로 11종의 나무이와 10종의 기주식물을 확인되었다. 귤나무가 속해있는 운향과를 기주로 하는 나무이는 발견되지 않았다.

This study was conducted to clarify the faunistic information of the genus Choristoneura in Korea. The genus Choristoneura belongs to the tribe Archipini of the family Tortricidae, including 46 species in the world. Among them, Choristoneura albaniana (Walker) is the only one species with a Holarctic distribution (Dang, 1992; Fagua et al., 2014). In Korea, 7 species of the genus Choristoneura have been reported up to date. But taxonomic location of some species in the genus are still confused: e.g. transfer of Choristoneura evanidana to Archips; Choristoneura simonyi to Xenotemna and so on. Also, it is necessary to identify correctly the genus Choristoneura, especially known as the forest pest. In this study, we rearrange and re-place the species, including nomenclatural changes according the current study.

The genus Docirava Walker, [1863] belongs to the tribe Chesiadini of the family Geometridae with only 12 described species in the world. The genus could be distinguished from other genera of Chesiadini with the characters, which had been described by walker in original description as follow : “Proboscis slender, rather long….Legs long, very slender; hind tibiae with four rather short and slender spurs”. In this study, the genus Docirava was first recorded with a new species Docirava sp. nov. with DNA barcode. Also, all the available information including images for both of genitalia and adult, wing venation and descriptional study was provided.

Limacodidae, also known as slug moths, comprise about 1,000 species worldwide (Epstein et al., 1998). This moth group is characterized by two adult characteristics: the presence of the female legs with the recessed pad of sensilla trichodea underneath and the entire, disk-shaped papillae anales, and four characteristics in immature stages: the absence of the crochets in all instars of larvae; the presence of the extended pupal maxilla, contiguous with the labial palpus; the hard, ovoid cocoons, lid invisible when uneclosed; and the flat, thin eggs (Epstein, 1996). Since Fixsen (1887), a total of 19 genera and 26 species of Limacodidae have been recorded from Korea. In the present paper, we reported three species of Limacodidae new to the Korean fauna: Ceratonema butleri Kawada, 1930, Microleon decolatus Sasaki, 2016, and Isopenthocrates japona Yoshimoto, 2004.

A nepticulid leaf-mine ichnofossil is described on the basis of a fossil leaf of Fagus from the early Miocene Geumgwangdong Formation (ca. 21–14 million years ago) in Pohang basin. This mine trace is characterized by a linear-blotch type with clear centric frass trail of closely and randomly dispersed pellets filling the mine width in early stage. We found traces of possible egg case and exit slit from the fossil. These features are most consistent with those produced by Nepticulidae. Our record represents the only reliably-identified nepticulid leaf-mine on Fagus in Miocene. Nepticulid leaf-mines in Miocene and the leaf-mine fossils from the Geumgwangdong Formation are briefly reviewed.