A diversification of external morphology and genital characteristics among conspecific animal populations that are geographically divided is particularly important to study the context of evolution and it is regarded as the early stage of allopatric speciation. The aim of the present study is to understand the morphological variations of Callipogon relictus Semenov from the three different populations (South Korea, China, Russia) in the Eastern Palaearctic region. A morphometric analysis was performed based on 87 selected external and internal characteristics from 95 specimens. As results of the present study, South Korean specimens shows remarkable morphological differences in genital characteristics compare to Russian ones. In Principal Component Analysis (PCA), South Korean and Russian populations are divided into two groups and the Analysis of Variance (ANOVA) analysis indicate that these two populations have significant differences in 14 morphometric characteristics.

Halyomorpha halys(Stål, 1855) (Hemiptera: Pentatomidae), also known as Brown Marmorated Stink Bug (BMSB), is a recent invasive pest in America and Europe. It originated from East Asia such as Korea, Japan, China, and Taiwan. Genus Trissolcus (Hymenoptera: Scelionidae) which is regarded as important species for biological control of H. halys, was described 6 species previously in Korea. But biology and taxonomic character of this genus is poorly known in Korea. Herein, we report two species from Korea, T. japonicus Ashmead, 1904 and T. plautiae Watanabe, 1954. We have investigated the morphological characters and COI sequence, and conducted an experiment about parasitic preference on BMSB's egg. Morphological key and picture of two species, and result of sequence investigation and experiment will be presented.

Surveillance for introduction of subtropical moths through the inanimate pathway in the Korean Peninsula was carried from 2010 to the 2015 using the burket (light) trap in Jeju island and a nearby dependent islands. Each record typically includes the taxonomic identity, its country of origin, and relevant biological information. According to a study, Lampronia flavimitrella (Hübner) belonging to the family Prodoxidae, from the first reported in Korea. Interestingly, erebids moth, Asota ficus (Fabricius) was distributed in the subtropical area from the southern part of China to Sumatra. It has been also mentioned that the personal blog on the internet was colleted from Yeosu and Mt. Yudal-san (JN), respectively. Especially, there was a crambids moth, Bocchoris inspersalis Zeller, on the origin of subtropical Africa South of the Sahara, but we can't at this point give any evidence about the intercepted status of this species in Korea. We continuously investigated the moth fauna in Jeju. However, its possible of establishment and spread in the Korean fauna can't be ruled out. Thus, it is very important to our weather conditions and further study on the surveillance of these species are needed, considering its possible status as invasive alien species. To resolve this question, further researches should be done to determine the pathway by which these subtropical moths occurred in Korea.

Even though plant protections using chemical pesticides have several advantages, non-specific toxicities to other beneficial insects and humans and rapid development of tolerance and/or resistance of target pests to chemicals are major disadvantages. Recent researches suggested that using double stranded RNA (dsRNA) could be species specific and environmental friendly pest management protocols. However, efficiency of dsRNA treatments are known to be variable according to its application methods. For example, injections of dsRNAs to pests were known to be effective in all species. However, efficiencies of oral application of dsRNAs were known to be dependent on species. Thus, development of tools that could enhance the efficacy of orally treated dsRNAs are utmost important for widening usages of dsRNAs in plant protection. Recently, we found that the efficacy of oral treated dsRNAs to target pests could be enhanced by nano-technologies. I will show how applying nano-technology to dsRNAs enhance the efficiency of dsRNAs. (This work was carried out with the support of the Cooperative Research Program for Agriculture Science & Technology Development (Project title: Studies for biological characteristics of and control methods against the migratory locust, Locusta migratoria, Project No: PJ011630042016), Rural Development Administration).

Drosophila has been used as a model for studying various human diseases. Especially, it has been widely used for studying neurological disorders in humans. For example, many progressive neurological disorders such as Alzheimer's diseases, Parkinson’s disease, Amyotrophic lateral sclerosis were recapitulated in Drosophila. We have been studying DYT1 dystonais, an enigmatic movement disorder. We generated several different kinds for Drosophila models by expressing human Torsin1A genes with or without causative mutations. In addition, we employed various Omics tools to identify any genetic, proteomic, and metabolomic alterations. I will summarize our recent progression in using Drosophil as a model for studying molecular and cellular etiologies underlying DYT1 dystonia and in vivo functions of Torsin proteins.

Four sympatric tortricid (Lepidoptera) species in New Zealand, Epiphyas postvittana (Walker), Planotortrix octo (Dugdale), Cydia pomonella (L.), and Cydia succedana (Denis and Schiffermüller), were studied for the morphology of antennal sensilla and the chemical communication systems using scanning electron microscopy (SEM), electro-antennogram (EAG), gas chromatography-electroantenno-detection (GC-EAD), single sensillum recording (SSR) and field tests. Antennal flagella of adult have four main types of olfactory sensilla (s.); s. trichodea, s. basiconica, s. auricillica, and s. coeloconica in the four species. The s. trichodea subtype I was longest and male-specific in all the species. The antennae of males of the four species showed characteristic higher EAG, GC-EAD and SSR responses to 12, 14-carbon acetates or alcohol. Some of the selected chemicals selected from the electrophysiological experiments caused significant antagonistic or agonistic activities to the sex pheromone of each species in the field. In this study, the four species showed distinct chemical communication systems according to sub-family, which possibly serve as a factor in species isolation.

Some Japonica rice plant varieties show the resistance to the whitebacked planthopper (S. furcifera) and the varieties produce ovicidal compound, benzyl benzoate, to kill their eggs lying in the rice plant. We have tried to elucidate the mechanism for inducing the production of the benzyl benzoate by the Japonica rice varieties. The active 80% MeOH/H2O extract was chromatographed on ODS open column and separated into 6 fractions; 100% H2O, 20% MeOH/H2O, 60% MeOH/H2O, 80% MeOH/H2O, 100% MeOH, 100% EtOH. Of these 6fractions, 100% MeOH and 100% EtOH fractions evidently induced benzyl benzoate at 43.95μg/g of fresh rice plant(frp) and 31.04μg/gfrp, respectively. The active compounds were submitted to analyses by using NMR and GC- and LC-MS inorder to elucidate the structure. We find elicitors to induce with S. furcifera. The elicitors are in the female of S. furcifera and the structures of elicitors are species of phospholipid and glycerol.

Plant-feeding insects are a ubiquitous feature of terrestrial life. It follows that understanding the evolution of insect herbivory and its diversity is fundamental to understanding the evolution of terrestrial ecosystems. During my Ph. D. study, I found phylogenetic conservatism in the feeding mode (internal vs. external feeding) of Yponomeutoidea, one of the primitive lepidopteran superfamilies, as well as in their diet breadth and in the growth form and taxonomic affinities of host plants they used. In spite of their importance in terrestrial ecosystems, the evolutionary history of Lepidoptera has been poorly known. This mostly is attributable to their poor fossil record. My Ph. D. study provided better understanding to the lepidopteran fossils. Fossils are an essential resource in divergence time estimation using molecular clock methods. Once reliably resolved, dating data can be used in tracing the evolutionary history of Lepidoptera/plant associations. I introduce my works to take such advantages from fossils and phylogenetic studies.

Leafhopper subfamily, Typhlocybinae (Hemiptera: Auchenorrhyncha: Cicadellidae), caused direct damage by sap-sucking or indirect damage by transmission of viruses or diseases on the host plants, so typhlocybinae is one of the important pest groups in the world. Typhlocybinae distribute worldwide and a total of 471 genera and more than 4,300 species have been described. so far, 38 genera and 102 species were recorded in the Korean Peninsula. In this talk, we provide several taxonomic studies for the Korean typhlocybene fauna based on the genus-level reviews of the subfamily from the Korean Peninsula.

Biological control has been tried for integrated pest management. It is often comparable, safe, and environment-friendly, making itself an alternative for chemical agents. Filamentous microorganisms, i.e., fungi and streptomystes, produce many kinds of useful metabolites, and some of them have been developed as a biocontrol agent. However, they still have a long way because of the concern of manufacturing cost. Therefore, process development was intensively studied to meet cost-effectiveness. Operating conditions of bioreactor, e.g., agitation and aeration, had an effect on biological and physiological responses such as mycelial morphology, oxygen and nutrient transfer. Understanding relationship between operating parameters and microbial responses in terms of growth, substrate and oxygen consumption, and production yield was critical for process development. This study dedicated to build strategies for mass production of biological control agent using aerobic filamentous microorganisms.

Chemical communication is very important for survival and reproduction in insects. The successful survival of insects depends to a large extent on the ability to detect and recognize environmental signals. We can manage the insect pest populations by eco-friendly methods by understanding the chemical communication systems which lie among the host trees, pests, and natural enemies. Persimmon is the largest fruit tree in acreage in Korea. Many insect pests are attacking various part of persimmon. Major insect pests of persimmon in Korea are Riptortus pedestris, Halyomorpha halys, Plautia stali as hemipterans, Stathmopoda masinissa, Euzophera batangensis, Conogethes punctiferalis as lepidopterans, There are also some minor insect pests including Homona magnanima, Apolygus spinolae, Synanthedon tenuis, bark beetles et cetera. Some of these produce sex pheromones to attract conspecific females or produce aggregation pheromones to gather conspecific individuals. Interestingly, some insect pests are using the semiochemicals of other species, pheromones or floral scents, as kairomones to find hosts or oviposition sites. These relationships in chemical communications at persimmon ecosystem will be discussed using our research results and others so far.

Insect antennae play important roles in finding mates and in locating food source and oviposition sites. Riptortus pedestris is an important pest of soybean and sweet persimmon in Korea. The male R. pedestris adult produce the aggregation pheromone attracting the conspecific nymphs and both sexes of adults. The pheromone was known as a cue for food finding, but the 1st instar nymph can develop to the 2nd instar without food. This phenomenon may suggest that the 1st instar nymph may have different sensilla system from other instars. Thus, we investigated the morphology and distribution of antennal sensilla and antennal response to the aggregation pheromone (AG) of each nymphal stage using Scanning Electron Microscope (SEM) and Electroantennography (EAG). As expected, first instar nymph did not have sensilla trichodea 3 (T3) and chaetica 3 (Ch3) which existed in other instar nymphs. The antennae of the 1st instar nymph did not responded to AG, with no difference from control. For further elucidation of the functions of sensilla T3 and CH3, single sensillum recording to AG will be done.

The purpose of this systematic review is to investigate the effects of tape application on improving body conditions. The search strategy for this review included a literature search by members of the International Kinematic Academy in 12 countries between January 2014 and February 2015 using PubMed, CINAHL, Cochrane, Google Scholar, websites and national journals. The search words included“Kinesiotape, Kinesio tape, kinesiotaping, elastic taping, taping, functional taping, myofascial taping, sensomotor taping”. The review included all articles, even those published in different languages. These searches resulted in 821 publications. There are several effects of tape application were revealed such as improving blood circulation, lymphatic circulation, body range of motion, activation of mechanoreceptor and joint stability, and decreasing pain. No one negative about the positive effects of taping, however the more effort is required to find evidence of effects of tape application.

The purpose of this study is to explore the effects of mobilization of C0-C1 and C7-T1 applied to asymptomatic individuals with reduced upper cervical rotation during the FRT. Design: parallel randomized controlled trial. 48 subjects(38.52 years±15.13) with C1-C2 rotation hypomobility in TFR joined the study and were randomized into three groups(C0, C7, control group). FRT in both directions was measured before and after the intervention. C0 intervention consisted of a dorsal translatoric mobilization of C0-C1 in the cervical neutral position. C7 intervention consisted of a ventral cranial translatoric mobilization of C7- T1 in neutral position and the control group maintained a supine position. C0 group experienced a FRT ROM to the restricted side increase of 17.64。(SD=4.55), that was significantly greater (P<0.001) than 5.95。 (SD=4.81) of the C7 group and 2.45。(SD=5.05) of the control group. The results showed that a dorsal translatoric mobilization of C0-C1 in neutral position restored the physiological FRT mobility in subjects with C1-C2 hypomobility and experienced statistical significant improvement in FRT as compared to a C7-T1 translatoric mobilization and a control group. (Level of evidence: 1b).

This study presents a potential environmental design, rather than the application of the environmental design technique model, to enhance the biodiversity of desolate space. Utilizing the main theories of landscape ecology in relation to vegetation and habitat restoration models, can allow for environmental restoration and the creation of climate change adaptation models to develop existing areas with specific topographical conditions, and allow for the utilization of the ecological base as a terrain recovery plan derived from the application of environmentally friendly design. We believe that the implications of this study will allow for the future creation of urban public parks and restoration of the ecological environmentally friendly design by using applied modeling techniques. Finally, we believe the suggestions outlined here provide a pilot case that delineates the value of the shared space as well appropriately address climate change.

Arsenic is an environmental pollutant and is known to induce toxicity. Arsenic acts on cells through a variety of mechanisms, influencing numerous signal transduction pathways. It induces a variety of cellular effects such as apoptosis, growth inhibition, promotion or inhibition of differentiation and angiogenesis. Arsenic has been associated with cancers of the skin, bladder, lung, kidney, and liver as well as with noncancerous conditions, diabetes and hepatopathy. However, arsenic trioxide (ATO) was reported to induce remission in patients with acute promyelocytic leukemia (APL). The anticancer efficiency of ATO was not restricted to APL; however, it has been extended to many solid tumors, including liver, cervical, prostate, lung, esophagus, and bladder tumors. Therefore, in this study, a number of previous studies are summarized that demonstrate arsenic-induced toxicity, including carcinogenesis and anticancer activity in vitro and in vivo. The arsenic metabolism that is involved in arsenic-induced toxicity is also discussed.

The work presented in this report was a detailed comparative study of the electrochemical response exhibited by graphite anodes in Li-ion batteries having different physical features. A comprehensive morphological and physical characterization was carried out for these graphite samples via X-ray diffraction and scanning electron microscopy. Later, the electrochemical performance was analyzed using galvanostatic charge/discharge testing and the galvanostatic intermittent titration technique for these graphite samples as negative electrode materials in battery operation. The results demonstrated that a material having a higher crystalline order exhibits enhanced electrochemical properties when evaluated in terms of rate-capability performance. All these materials were investigated at high C-rates ranging from 0.1C up to 10C. Such improved response was attributed to the crystalline morphology providing short layers, which facilitate rapid Li+ ions diffusivity and electron transport during the course of battery operation. The values obtained for the electrical conductivity of these graphite anodes support this possible explanation.

This paper addresses the effect of dopants on the electronic properties of zigzag (8, 0) semiconducting single walled carbon nanotubes (SWCNTs), using extended Hückel theory combined with nonequilibrium Green’s function formalism. Through appropriate dopant concentrations, the electronic properties of SWCNTs can be modified. Within this context, we present our ongoing investigation on (8, 0) SWCNTs doped with nitrogen. Quantum confinement effects on the electronic properties of the SWCNTs have also been investigated. The obtained results reveal that the electronic properties of SWCNTs are strongly dependent on the dopant concentration and modification of electronic structures by hydrogen confinement.

Mesenchymal stem cells (MSCs) are multipotent stem cells, which can be induced to differentiate into several cells. MSCs are also reported to possess immunomodulatory properties through secretion of inflammatory cytokines and generation of regulatory T (Treg) cells. Treg cells play an important role in allergic disorders, including atopic dermatitis. We examined the immunomodulatory effects of canine adipose tissue derived-MSCs (cAD-MSCs) in 3 groups: Group 1, untreated normal dog; Group 2, dogs with Dermatophagoides farinae ointment-induced atopic dermatitis; and Group 3, dogs with atopic dermatitis. Canine peripheral blood mononuclear cells (PBMCs) were collected from each group and co-cultured with cAD-MSCs. After co-culturing, PBMCs were separated and the expression of Treg cells was examined by flow cytometry. According to the results, the populations of Treg cells were increased in 3 ex vivo experimental groups, co-cultured with cAD-MSCs. These results would be important for the application of MSCs in clinical trials.

Garlic (Allium sativum) is known to have antimicrobial effects but there are few data on its effects against fish pathogenic bacteria. This study was conducted for possible development of natural food antimicrobials from garlic extracts, particularly allicin. The filter sterilized, aqueous extract of garlic was tested for its ability to inhibit the growth of 7 fish pathogenic bacteria (E. tarda, V. ichthyoenteri, V. harveyi, P. damselae, S. iniae, S. parauberis, L. garviae) using the disc diffusion test, minimum inhibitory concentration test, and minimum bacteriocidal concentration test. The garlic extract inhibited growth and killed most of the organisms tested. The disc diffusion test was performed using garlic extracts (100%, 50%, 25%, 10%, each 20㎍), and MIC test was also performed using a diluted concentration of garlic extracts (488 - 250,000 ㎍/㎖). According to the results, garlic extracts exhibited antimicrobial activity against all of seven bacteria, and these results suggest that this garlic extract can be used as an antimicrobial substance against fish bacterial diseases in the fish industry.