Neoporphyra kitoi Ma. Abe, N. Kikuchi, Tamaki, Tom. Sato, Murase, Fujiyoshi & Mas. Kobayashi has been known as an endemic species in Japan. Its high temperature tolerance suggests that it could be advantageous for cultivation. In this study, we collected it from the Ulleungdo island, Korea and transferred it into Pyropia for a new combination, identified as Pyropia kitoi (Ma. Abe, N. Kikuchi, Tamaki, Tom. Sato, Murase, Fujiyoshi & Mas. Kobayashi) D.J. Kim, T.O. Cho & B.Y. Won comb. nov. based on morphological and molecular analyses. Pyropia kitoi is also reported as a new record species in the list of Korean macroalgal flora. Although we didn’t observe the emergence of new blades from the rhizoidal cells, which is a key character for this species, our molecular analysis of rbcL revealed that our samples from Korea were congruent with “Neoporphyra kitoi” from Japan and were nested within the clade of Pyropia. The gene sequence divergence between the Korean and Japanese samples was 0-0.2%.

Recently, defects that occurred during the construction of apartment houses have become a social issue. Defects in apartment houses lead to waste of resources and economic loss, causing psychological and physical damage to customers, and a decrease in reliability and financial loss to construction companies. The purpose of this study was to analyze the impact of defect occurrence according to the difference in project management level in the apartment housing construction stage, and to investigate the extent to which the project manager's experience affects the defect occurrence rate. For the empirical analysis of the study, statistical analysis was conducted using data collected from 130 actual projects. The results of the analysis showed that schedule management, cost management, and quality management had a positive effect on reducing the defect occurrence rate in the execution stage of apartment housing construction, while human resource management productivity had a negative effect. This study theoretically demonstrated the importance of project management, and in practice, showed that schedule management, cost management, and quality management should be faithfully performed in the execution stage to reduce the rate of defects after project completion. It was also suggested that hiring an experienced project manager would help reduce project defects.

Background: The grading of Hanwoo (Korean native cattle) is based on four economic traits, and efforts have been continuously made to improve the genetic traits associated with these traits. There is a technology to predict the expected grade based on the 4 economic genetic SNP characteristics of Korean cattle calves using single nucleotide polymorphism (SNP) technology. Selection of highly proliferative, self-renewing, and differentiating satellite cells from cattle is a key technology in the cultured meat industry. Methods: We selected the Hanwoo with high and low-scored of genomic estimated breeding value (GEBV) by using the Hanwoo 50K SNP bead chip. We then isolated the bovine satellite cells from the chuck mass. We then conducted comparative analyses of cell proliferation, immunocytochemistry, qRT-PCR at short- and long-term culture. We also analyzed the differentiation capability at short term culture. Results: Our result showed that the proliferation was significantly high at High scored GEBV (Hs-GEBV) compared to Low scored GEBV (Ls-GEBV) at short- and long-term culture. The expression levels of Pax3 were significantly higher in Hs-GEBV bovine satellite cells at long-term culture. However, there were no significant differences in the expression levels of Pax7 between Hs- and Ls-GEBV bovine satellite cells at short- and long- term culture. The expression levels of MyoG and MyHC were significantly high at Ls-GEBV bovine satellite cells. Conclusions: Our results indicated that selection of bovine satellite cells by Hanwoo 50K SNP bead chip could be effective selection methods for massive producing of satellite cells.

The advent of big data has brought about the need for analytics. Natural language processing (NLP), a field of big data, has received a lot of attention. Topic modeling among NLP is widely applied to identify key topics in various academic journals. The Korean Society of Industrial and Systems Engineering (KSIE) has published academic journals since 1978. To enhance its status, it is imperative to recognize the diversity of research domains. We have already discovered eight major research topics for papers published by KSIE from 1978 to 1999. As a follow-up study, we aim to identify major topics of research papers published in KSIE from 2000 to 2022. We performed topic modeling on 1,742 research papers during this period by using LDA and BERTopic which has recently attracted attention. BERTopic outperformed LDA by providing a set of coherent topic keywords that can effectively distinguish 36 topics found out this study. In terms of visualization techniques, pyLDAvis presented better two-dimensional scatter plots for the intertopic distance map than BERTopic. However, BERTopic provided much more diverse visualization methods to explore the relevance of 36 topics. BERTopic was also able to classify hot and cold topics by presenting ‘topic over time’ graphs that can identify topic trends over time.

This study was conducted to determine the appropriate seeding dates by verifying the difference in winter survival and productivity of alfalfa according to fall sowing dates in the central area of South Korea. The experiment was conducted for 2 years (2020 and 2021) at the field in the Department of Animal Resources Development, NIAS located in Cheonan. Sowing dates started from September 18 to November 8 with 10 days of intervals during 2020 and 2021; SO1 (September 18), SO2 (September 28), SO3 (October 8), SO4 (October 18), SO5 (October 28), and SO6 (November 8). After sowing, the winter survival rate was measured in the spring of the following year, and the dry matter yield was measured by harvesting at 10% flowering and harvesting five times a year. SO6 failed to winter survival, and SO5 also had a lower winter survival rate than SO1~4 (p<0.05). The average annual dry matter yield of alfalfa linearly decreased with delaying sowing dates (p<0.05). The feed value did not differ in the same year by delaying the sowing date in the same year. These results suggest that sowing date should be started before October 18 to increase winter survival and productivity of alfalfa in the central area of South Korea.

As increasing number of stray cats, it recently has more chance for stray cats to invade people area. Inevitably, the number of cases of inconvenience and damage caused by street cats is also increasing proportionally. Therefore, we investigated and analyzed the current status and implementation of TNR in Korea and suggested the direction in which Korea's policy should go. Domestic TNR has been implemented for about 20 years, but the ratio of stray cats to which TNR is applied insufficient and post-management after TNR is also insufficient. In the case of other countries, a large percentage of TNR is conducted by selecting TNR target cats based on scientific grounds and adoption or additional observation is carried out after TNR. Based on this strategy, the conditions for successful TNR need to expand the decision-making authority of veterinarians involved in TNR and actively invigorate subsequent solutions such as adoption. In addition, the policy should be improved in the way of expanding management and supervision after TNR through food service centers and shelters.

Background: The potential impact of aqueous extracts from Psidium guajava leaves on the reproductive system of female rabbits was evaluated. Methods: Twenty-eight rabbits, aged five to six months were utilized. Rabbits were divided into four groups and were randomly assigned to receive one of the following oral doses of the guava leaf extracts: 0 (control group), 10, 20, or 30 mg/kg of body weight. After a treatment period of 30 days, blood was collected via jugular venipunture and the serum was extracted for the assessment of serum biochemical traits levels. The females were bred and monitored throughout their pregnancy to ascertain reproductive outcomes. Results: The results indicated that the guava leaf extract significantly increased the body weight of the rabbits during both pre- and post-pregnancy compared to the control group (p < 0.05). The litter size at three weeks post-birth, prolificity rate, FSH, LH, and protein levels were notably higher (p < 0.05) at a dose of 20 mg/kg of body weight. The viability rate three weeks post-birth increased with escalating extract doses, and the highest values were observed at doses of 20 and 30 mg/kg of body weight (p < 0.05). Conclusions: This study demonstrated that, the aqueous extract of guava leaves appears to stimulate the production of FSH, LH and enhance body weight, prolificity, and pregnancy outcomes in mammals. As such, it is suggested that a dose of 20 mg/kg body weight could be beneficial in improving the reproductive performance of female.

Ischemic stroke leads to severe brain damage and high mortality. Chlorogenic acid is a phenolic compound known to have neuroprotective properties. Bcl-2 family protein plays an important role in the regulation of apoptosis. We investigated whether chlorogenic acid exerts neuroprotective effects against ischemic injury by modulating Bcl-2 and Bax proteins. Middle cerebral artery occlusion (MCAO) was performed to induce cerebral ischemia and rats were injected intraperitoneally with phosphate buffered saline or chlorogenic acid (30 mg/kg) for 2 h after MCAO. Cortical tissues were collected 24 h after MCAO injury and reverse transcription-quantitative real time polymerase chain reaction and Western blot analyses were performed to investigate the expression of Bcl-2 and Bax. The regulation of Bcl-2 and Bax proteins by chlorogenic acid during glutamateinduced cell damage were examined. Cells were collected at 24 h after administration of glutamate (5 mM) and chlorogenic acid (10, 30, 50 μM). These results showed a decrease in Bcl-2 expression and an increase in Bax expression in MCAO animals, but chlorogenic acid treatment alleviated these changes by MCAO damage. Glutamate significantly reduced cell viability, and chlorogenic acid treatment alleviated this reduction in a dose-dependent manner. Glutamate induced a decrease in Bcl-2 expression and an increase in Bax expression, but chlorogenic acid treatment alleviated these changes. We found that chlorogenic acid alleviates changes in the expression of Bcl-2 and Bax proteins induced by brain injury. Therefore, our findings provide an evidence that chlorogenic acid has neuroprotective effects against MCAO damage by modulating Bcl-2 and Bax proteins.

Ischemic stroke causes severe neuronal damage. Chlorogenic acid is a phenolic substance present in fruits and coffee. It also exerts neuroprotective effects against various brain injuries. The 14-3-3 family protein perform a variety of functions including metabolism, signal transduction, cell differentiation, and apoptosis. The purpose of this study is to investigate whether chlorogenic acid regulates the expression of 14-3-3 protein in stroke animal models. Ischemic stroke was induced by middle cerebral artery occlusion (MCAO) surgery. Phosphate buffered saline (PBS) or chlorogenic acid (30 mg/kg) were intraperitoneally injected to adult male rats 2 h before MCAO surgery. Adhesive-removal test was performed 24 h after MCAO surgery and cerebral cortical tissues were collected for further study. MCAO damage caused severe neurological impairment and chlorogenic acid treatment ameliorated this disorder. Our proteomic approach showed a decrease in 14-3-3 expression in MCAO animals with PBS. The decrease in 14-3-3 expression alleviated in MCAO animal with chlorogenic acid. We confirmed changes in various 14-3-3 protein isoforms, including beta/alpha, zeta/delta, gamma, epsilon, eta, and tau through reverse transcription-PCR. These results explained that chlorogenic acid regulates the expression of 14-3-3 protein in MCAO-induced cerebral ischemia. 14-3-3 is considered to be an important protein for cell survival through binding to pro-apoptotic proteins. The maintenance of 14-3-3 levels is an important event in neuroprotection against ischemic injury. Therefore, we can demonstrate that the 14-3-3 protein contributes to the neuroprotective effect of chlorogenic acid in stroke animal models.

This study investigated ethopabate (EPB) residues in edible tissues of broiler chickens given in drinking water and established the withdrawal time (WT) of EPB in poultry tissues. Twenty-four healthy Ross broiler chickens were orally administered with EPB at the concentration of 3.8 mg/L for 14 days (EPB-1, n=24) and 15.2 mg/L for 7 days (EPB-2, n=24) through drinking water, respectively. After the drug treatment, tissue samples were collected from six broiler chickens at 0, 1, 3, and 5 days, respectively. EPB residue concentrations in poultry tissues were determined using LC-MS/MS. Correlation coefficient values ranged from 0.9980 to 0.9998, and the limits of detection and quantification (LOQ) were 0.03~0.09 and 0.1~0.3 μg/kg, respectively. Mean recoveries in muscle, liver, kidney and skin/fat tissues were 95.9~109.8, 108.7~115.3, 89.9~96.6 and 86.7~96.8%, respectively, and coefficient of variations were less than 17.11%. At the end of the drug-administration period (0 day), EPB was detected at levels under the LOQ in all tissues from both the EPB-1 and EPB-2 groups. According to the results of EPB residue in Ross broiler tissues, withdrawal periods of both EPB-1 and EPB-2 in poultry tissues were established to 0 day. In conclusion, the developed analytical method is suitable for the detection of EPB in poultry tissues, and the estimated WT of EPB in poultry tissues will contribute to ensuring the safety of Ross broiler chickens.

A 12-year-old intact female Schnauzer was referred for the evaluation of poorly controlled diabetes mellitus: despite insulin therapy, blood glucose concentration was consistently high, indicating a decreased insulin sensitivity. Laboratory analyses revealed persistent hyperglycemia, glucosuria, and ketonuria. Diagnostic approaches were performed to identify concurrent disorders that can cause insulin resistance. The dog was found to have concurrent hyperadrenocorticism, hyperlipidemia, pancreatitis, and vaginal cytology indicating diestrus in the estrus cycle. Trilostane administration for hyperadrenocorticism improved the insulin response; however, the dog remained hyperglycemic. Eventually, the dog showed complete remission without insulin administration 1 week after the ovariohysterectomy. The dog remained in remission for approximately 4 months, but eventually relapsed and the condition was permanent. Diestrus in intact females and hyperadrenocorticism are known to be the two main causes of insulin resistance in dogs. After the management of these conditions, the dog achieved diabetes remission, which rarely achieves in dogs. In cases of insulin resistance, such as hormonal imbalances or inflammatory conditions, remission can be achieved by addressing the underlying cause. Hence, it is important to assess the presence of comorbidities associated with insulin resistance in dogs with poorly controlled diabetes mellitus and to treat each condition as soon as possible.

Background: The regulation of maternal immunity is critical for the establishment and maintenance of successful pregnancy. Among many cell types regulating the immune system, innate lymphoid cells (ILCs) are known to play an important role in innate immunity. Although some reports show that ILCs are present at the maternalconceptus interface in humans and mice, the expression and function of ILCs in the endometrium have not been studied in pigs. Methods: Thus, we determined the expression, localization, and regulation of ILC markers, CD127 (a common marker for ILCs), BCL11B (a ILC2 marker), and RORC (a ILC3 marker) at the maternal-conceptus interface in pigs. Results: The expression of BCL11B and RORC, but not CD127, in the endometrium changed during pregnancy in a stage-specific manner and the expression of CD127, BCL11B, and RORC was greatest on Day 15 during pregnancy. CD127, BCL11B, and RORC were also expressed in conceptus tissues during early pregnancy and in chorioallantoic tissues during the later stage of pregnancy. BCL11B and RORC proteins were localized to specific cells in endometrial stroma. The expression of CD127 and BCL11B, but not RORC, was increased by the increasing doses of interferon-γ (IFNG) in endometrial explants. Conclusions: These results suggest that ILCs present at the maternal-conceptus interface may play a role in the establishment and maintenance of pregnancy by regulating the innate immunity in pigs.

Recently, carbon composites have been applied to various fields. However, carbon composites have not been applied to the fishing vessel field due to its structure standards centered on glass composites. In this study, a structural strength evaluation study was conducted for the application of carbon composites in the fishing vessel field. Hull minimum thickness verification test and hull joint verification test were conducted. Compared to glass composites, the verification was based on equivalent or better performance. The results show that carbon composites can reduce the weight by 20% compared to glass composites. For hull joints, it was necessary to increase the thickness of the joint seam by the thickness of the hull to apply carbon composite. Through this study, a standard for the application of carbon composites to fishing vessel can be established.

User churn in games often arises due to inadequate game difficulty. To address this, non-player characters (NPCs) has been utilized to modulate difficulty according to individual game skill. Nevertheless, the effectiveness of solely NPC-based adjustments is limited since game difficulty is influenced by both NPCs and environmental factors. This paper introduces a novel method for dynamically tailoring game difficulty by adjusting in-game environments based on player behavior patterns in top-down shooter game. Through analysis of diverse user game play data, we find that factors within the game environment, such as the distribution of enemy characters and the arrangement of terrain, have a substantial influence on the level of difficulty. Furthermore, it has been observed that behavioral patterns of players show variations according to changes in the game environment. Using these analytical result, we devise an artificial neural network model that configures an environment that suit player behavior patterns. With the model, we figure out the user player pattern and control the difficulty dynamically by changing the environment factors. Through the experiments, we show that our method provides an appropriate level of difficulty for users to prevent user churn.

본 연구는 금속 인공물을 감소시키기 위한 VAT(view angle tilting)와 SEMAC(slice encoding for metal correction) 기법 적용에 따른 온도 변화 범위를 관찰하고자 하였다. 제작된 인체 모방 팬텀을 활용하였고, 검사방법으로는 임상에서 실제로 사용하고 있는 고속스핀에코(fast spin echo, FSE) 기법의 영상 파라미터들을 그대로 이용하였다. VAT 와 SEMAC 기법은 FSE와 같은 파라미터로 설정한 다음 VAT 파라미터는 100%와 SEMAC 파라미터는 25로 설정하였다. 온도 측정 방법으로는 수소원자 공명주파수전이법(proton resonance frequency shift, PRFS)기법을 활용하였으며, 광 섬유 온도계(fiber-optic sensor, FOS)로 절대 온도를 측정한 후 비교 분석하였다. 온도 변화는 SEMAC 기법에서 기존 FSE 기법 (0.28℃±0.10℃)에 비해 1.63℃±0.12℃로 약 6배 상승하였고(SEMAC-FOS = 1.59℃), VAT 기법은 약 2배 증가(VAT-FOS = 0.51℃)가 확인되었다. 특히, SEMAC 기법은 VAT 기법(VAT-FOS = 0.51℃, VAT-PRFS = 0.54℃ ±0.02℃)과 비교하여 약 3배가 증가하여 가장 높은 온도 상승이 관찰되었다. 이는 SEMAC 기법 적용 시 자기공명영상 전자파 인체 영양에 대한 안전기준을 충족하기 위해 영상 파라미터 최적화 작업의 필요성을 시사한다.