Background : Cordyceps militaris has been an wonder drug to anti-aging efficacy and called the three main drugs with ginseng and deer antler from the past. Cordycepin, cordycepic acid (d-mannitol) and adenosine are known as functional ingredients in Cordyceps militaris. Among them, cordycepin, the representative component, has been reported as antimicrobial substance containing immune enhancement, anti-cancer and anti-inflammatory effects. Methods and Results : After Cordyceps militaris produced from different types of medium mixed with 10-fold volume of purified water, the mixture were extracted at 70±5℃ for a hour and that extracts re-extracted using ultrasonics wave for 30 minutes. Qualitative analysis of the index component was determined by using the Q-TOF (A quadrupole time-of-flight mass spectrometer), and quantitative analysis was performed by using HPLC (high-performance liquid chromatography) with Xselect HSS T3 column (2.1 X 100 mm, 2.5㎛, Waters, USA) and ultrapure water and acetonitrile as mobile phase A and B. Detection column temperature, injection volume and the flow rate were 35℃, 2 μL and 0.3 mL / minute respectively. The cordycepin content of Cordyceps militaris produced from medium mixed with vegetable and animal ingredients higher than single ingredient. Moreover, through a variety of analyzes by varying the type and content of the medium additives, the cordycepin in Cordyceps militaris produced from medium mixed with animal ingredients highest. Furthermore, the cordycepin content of a fruit body was higher than those of the a mycelium. Conclusion : These results provide a method for producing an high cordycepin content of Cordyceps militaris as functional food ingredient.

Background : Platycodon grandiflorum is a perennial plant and a member of Camanulaceae family. Since ancient times, they have been using P. grandiflorum as an important medicinal plant in Korea. Platycodin D is the most abundant saponin derived from P. grandiflorum and pharmacologically active component. UDP-glycosyltransferases (UGTs) are important enzymes in the saponin biosynthesis. UGT is a glycosyltransferase and act on the final step of the secondary metabolite biosynthesis. Methods and Results : We tried to identify UGT genes related to saponin biosynthesis of P. grandiflorum through RNA-seq analysis. The sequencing was performed using Illumina Hi-Seq platform after cDNA library preparation. The produced reads were assembled using CLC Genomics Workbench software (CLC Bio, Inc.). We obtained 122,663 contigs and found 137 putative UGT genes. Familes of UGT71, UGT73, and UGT74 were selected as putative saponin biosynthesis related gene families using phylogenetic relationship analysis. qPCR condition about UGT73 is preheating 94℃ 180 sec, denaturation 94℃ 60 sec, annealing 53℃ 60 sec, extension 72℃ 90 sec, final extension 72℃ 600 sec, 45 cycles repeated. Conclusion : The results in this study could help to find the UGTs related to saponin biosynthesis pathway of P. grandiflorum.

Background : Tetraploid plant is bigger in size of organs like leaf, stem, fruit, and root than diploid plant by chromosome doubling. Also, Self-ìncompatibílity disappeares and disease resistance is strengthened in tetraploid plant. To breed new boxthorn variety having bigger fruit, higher yield, pest tolerance, and, self-compatibílity, tetraploids was induced for tetraploid breeding resources by colchicine treatment in each boxthorn variety. Materials and Methods : Colchicine was treated in Myongan, Cheongdae, Bulro, and Youngha for 0.1% concentration and 48 hours. Polyploid was identified by flow cytomertry(Partec, PA-1) and chromosome numbers of polyploid individuals were examined by aceto-carmine stain. Conclusion : Colchicine was treated in Myongan, Cheongdae, Bulro, and Youngha for 0.1% concentration and 48 hours. Tetraploid was induced as 13, 9, 6, and 5 individuals in Myongan, Cheongdae, Bulro, and Youngha respectively by above colchicine treatment.

Background : Recently new breeding goal setting is required to develop new boxthorn variety with superior treats of bigger fruits, higher yield in fruits and sprout, pest tolerance, higher medicinal functionality, and self-compatiblity. Various genetic resources also should be retained for this goal. This study was carried out to obtain boxthorn tetraploid for breeding resource. Methods and Results : Colchicine concentration and treatment time for polyploid induction were 0.1, 0.2, 0.4, 0.6% and 12, 24, 36, 48hours respectively. Polyploid was identified by chromosome numbering of individuals by aceto-carmine stain which were examined for polyploid by flow cytomertry(Partec, PA-1) in advance. Conclusion : When cuttings of Cheongyang-jaerae were treated by colchicine to induce tetraploid, survival rate decreased as colchicine concentration and treatment time increased. Rate of individuals having over 30 chloroplast number of stomata guard cell was highest as 37.5% in colchine 0.1% and 48 hours treatment. 8 of them were identified as polyploid by flow cytometry and tetraploid induction efficiency was highest as 6.0% in colchine 0.1% and 48 hours treatment. According to chromosome number examination, 3 of 8 individuals were aneuploid and 5 were tetraploid.

Background : Astragalus membranaceus is one of the most widely used traditional medicinal herbs in Korea. Studies on the genomic of A. membranaceus resources have not been carried out so far. The present study was carried out to discriminate A. membranaceus based on genetic diversity using genomic simple sequence repeat (SSR) markers. Methods and Results : We collected 5 A. membranaceus lines: Asung, Poongsung, Am-Jecheon, Am-Sancheong, and Am-China. One hundred mg of fresh leaves were used for genomic DNA extraction using the DNeasy plant DNA isolation kit (Qiagen GmbH, Hilden, Germany). 450,449 contigs were searched for 147,766 SSR candidate loci in this study using the MicroSAtellite identification tool (MISA). We selected 949 A. membranaceus genomic SSR markers that were showed variation for the five collections in silico screening with CLC genomics workbench program. The genetic diversity of all A. membranaceus resources was analyzed using 17 SSR markers employing the DNA fragment analysis method. Based on the genetic diversity analysis, these lines were classified into four distinct groups. Conclusion : These findings could be used for further research on cultivar development using molecular breeding techniques and for conservation of the genetic diversity of A. membranaceus. Furthermore, the markers could be used for marker-assisted selection for crop breeding.

Background : Clematis trichotoma is a deciduous climber belonging to the family Ranunculaceae. This study was carried out to survey the effect of shading levels on growth characteristics in Clematis trichotoma seedling. Methods and Results : Seeds were collected from plants growing in the Mt. Kariwang Jeongseon, Gangwon-do in October 2013, and they were sown to 96 cell plug tray filled with Peatmoss (TKS-2) soil at March, 2015. The experiment was performed with four different shading levels (0, 30, 60, 90%) at July, 2015. According to the experiment, plant height was the highest under 90% of shading. It was found that fresh weight and dry weight of clematis trichotoma were the highest under 90% of shading. The leaf number was the highest under 30% of shading. The leaf number decreased as the shading level increased. Root number and length were the highest under 90% of shading. Conclusion : According to the results, Clematis trichotoma seedling showed the highest growth under 90% shading.

Background : Scrophularia koraiensis is a herbaceous perennials belonging to the family Scrophulariaceae. This study was carried out to survey the effect of LED on root develop characteristics in Scrophularia koraiensis seedling. Methods and Results : Seeds were collected from plants growing in the Mt. Kariwang Jeongseon, Gangwon-do in September 2014, and they were sown to 128 cell plug tray filled with Peatmoss (TKS-2) soil at March, 2015. The experiment was performed with three different LED (Blue, Red, Blue + Red) at July, 2015. Morphological characteristics of root (total root length, root projet area, root surface area, root diameter and root volume) were analyzed with WinRHIZO software. Seedling root growth of scrophularia koraiensis was surveyed to be the highest at the Blue + Red LED in all measuring. Total root length was measured high in the order of Blue + Red, Red, Blue LED. Conclusion : According to the results, Scrophularia koraiensis seedling showed the highest root growth in Blue + Red LED.

Background : Jiwhang (Rehmannia glutinosa L.) cultivation area of chungnam province was 62ha(219 farms, 53.9% of domestic cultivation area, M.A.F.R.A. 20142)). Recently, jiwhang is rapidly spread to 150ha ( Geumsan administration statistics, 2015) as taking place of income crop in Chungnam. High quality jiwhang GAP production and improvement of the distribution system including magnus customers, producer association fosterage and conventional market Vitalization were the reason of rapid cultivation area increase of Rehmannia glutinosa L. in Geumsan. This study was performed to set up the standard Rhizome size for planting in Rehmannia glutinosa L. Materials and Methods ○ Experiment variety : Togang ○ Treatment(rhizome length) : ① 1cm ② 2cm ③ 3cm ④ 4cm ⑤ 6cm(control) ○ Planting date : May 6, 2015 ○ Planting distance : 30×15cm ○ Experiment place : Ginseng and Medicinal Plant Research Institute (Geumsan-gun, Chungcheongnam-do) Conclusion : In the aboveground growth was no significant difference among treatments. In the root characteristics, there was no significant difference among treatments. Take a 1∼2cm rhizome planting saved rhizome amount and planting time to 73.8%∼64.3% and 68.4%∼ 57.8% respectively than 6cm(control).

Background : Generally, price of Jiwhang(Rehmannia glutinosa L.) is highest in August. In this study, Economic Evaluation and Productivity of 9 Jiwhang varieties w ere analyzed according to harvesting tim es of August and October. Materials and Methods ○ Experiment variety : Jiwhang 1, Korea jiwhang, Daegyung, Gogang, Togang, Dagang, Wongang, Hwanggang and Yeongang ○ Treatment : ①Initial-August harvest ②Mid-August harvest ③Ended-August harvest ④Ended-October harvest ○ Planting date : April 17, 2015 ○ Planting distance : 30×15cm ○ Experiment place : Ginseng and Medicinal Plant Research Institute (Geumsan-gun, Chungcheongnam-do) Conclusion : The higher roots yield at August harvest was in Gogang, Dagang and Togang. The lower roots yield at August harvest was in Korea jiwhang and Hwanggang. The higher economic feasibility at August harvest was in Gogang, Togang, Dagang and Wongang. The higher economic feasibility at ended-October harvest was in Gogang, Yeongang, Togang, Dagang and Wongang.

Background : According to medicinal plant standard culture, flower stalk should be removed at the stage of 4-5 main leaves appearance but there is no detail information on stalk removal in jiwhang (Rehmannia glutinosa L.). This study was carried out to examine effect of flower stalk removal on the increase of total yield in Rehmannia glutinosa L. Materials and Methods ○ Experiment variety : Korea jiwhang, Wongang ○ Treatment : ① Peduncle non-cutting ② Peduncle cutting ○ Planting date : April 18, 2015 ○ Planting distance : 30×15cm ○ Experiment place : Ginseng and Medicinal Plant Research Institute (Geumsan-gun, Chungcheongnam-do) Conclusion : Aerial part growth was no significant difference according to treatments in Wongang and aerial weight was havier in Peduncle cutting than in Peduncle non-cutting. Aerial part growth was no significant difference according to treatments in Korea jiwhang. The roots yield in Korea jiwhang was increased about 5.2% in Peduncle cutting. The roots yield in Wongang was increased about 24.9% in Peduncle cutting.

Background : Bai Zhu is generally harvested in 1-2 years after seedstock planting. Recently mainly in Guemsan, Bai Zhu is harvested in just 1 year after planting in spring . According to medicinal plant standard culture, Bai Zhu should be directly sown by 20cm distance in April. This study was carried out to find out proper planting date. Materials and Methods ○ Experiment variety : Bai Zhu (Atractylodes macrocephala Koidz.) ○ Treatment : Planting Date ① Late march ② Early april ③ Middle of april(control) ④ Late april Plantind density : ① 30×10cm ② ① 30×20cm ○ Planting type : Direct Seeding ○ Experiment place : Ginseng and Medicinal Plant Research Institute (Geumsan-gun, Chungcheongnam-do) Conclusion : Number of lateral branches in 30×10cm was more in late march than late April and aerial part growth was superior as planting date was earlier. Emergence rate was higher in middle of April and late April and aerial part weight, fresh root weight, dry root weight rate, and dry root weight were superior as planting date was earlier. Dry weight yield increased as 38.3% in late March compared to middle of April. Dry weight was havier as 36.2% in planting time of late March and early April than planting time of middle of April and late April. Total income was higher in planting time of late March and early April than planting time of middle of April and late April.

Background : Jiwhang (Rehmannia glutinosa L.) cultivation area of Chungnam province was 62ha (219 farms, 53.9% of domestic cultivation area, MAFRA. 20142)**). Recently, jiwhang is rapidly spread to 150ha (Geumsan administration statistics, 2015) as taking place of income crop in Chungnam. High quality jiwhang GAP production and improvement of the distribution system including magnus customers, producer association fosterage and conventional market vitalization were the reason of rapid cultivation area increase of Jiwhang in Geumsan. But low emergence rate caused by spring drought should be solved. This study was carried out to obtain basic data for emergence rate improvement in jiwhang. Materials and Methods ○ Experiment variety : Korea jiwhang ○ Treatment : ①Control ② 1 time irrigation at planting time ③1 time irrigation at planting time + transparent vinyl mulching ○ Planting date : April 30, 2015 ○ Planting distance : 30×15cm ○ Experiment place : Ginseng and Medicinal Plant Research Institute (Geumsan-gun, Chungcheongnam-do) Conclusion : There was no significant difference in the aboveground growth among treatments. The seedling emergence rate was higher in 1 time irrigation at planting time + transparent vinyl mulching(1ITVM) than control or 1 time irrigation at planting time(1I). Aerial part weight was significantly havier in 11ITVM than control or 1I. The roots yield was also more in 1ITVM than control or 1I. According to economic analysis, total income in 1ITVM compared to 1I and control was 125.1% and 54.0% higher respectively.

Background : Curcuma longa L., is a very famous spice and medicinal herb in several countries of the world. Curcumin and volatile oils of C. longa improve hepatic and renal functions, and could be used against biliary disorders, diabetic and hepatic disorders. This medicinal plant was introduced for the regional promotion, Jeollanam-do, as the subtropic area in Korea. In the present study, the plant was introduced to the Jeollanam-do region of Korea with the aim to determine the optimal planting densities for growth characteristics, yield and curcumin contents which have not been reported up until now. Methods and Results : The field experiment was conducted at the experimental farm of Division of Traditional Korean Medicine Resource, NIKOM, Jangheung-gun, Jeollanam-do, Korea. The seedstock was planted at 40×20cm, 40×30cm and 40×40cm spacing with 3 replications. Nine plants were selected randomly for observations on growth characteristics such as plant height, stem diameter, leaves number, leaves area and to dry weight at days after transplanting (DAT) 90, 120 and 150. The dried samples of plant were stored in air tight polythene bags at room temperature for HPLC analysis of curcumin, which was undertaken following standard procedures. Top plant growth was observed in the 40×30cm planting density of C. longa, plant height, leaves number, leaves area and day shoot weight were larger than in the other treatments. Root length, root diameter and supporting roots of underground part were increased as planting density was wide, and dry root weight was increased in 40×30cm. However, yield and curcumin constituent was highest each 294 kg/10a and 68.7 mg/g in the planting density of 40×20cm. Conclusion : Based on the results of this study, planting density of 40×20cm is the most suitable in the cultivation and curcumin constituents of C. longa.

Background : Liriope platyphylla Wang et. Tang is a perennial plant and its tuberous root is used as herbal medicine. Liriope platyphylla is grown throughout a year from April to April of next year. Recently, the production was lowered because of severe drought/rainfall and injury by successive cropping. The ridge height affected the growth of shoots and roots as affected by drought or rainfall. It is necessary to establish the cultivation techniques for stable quantity per unit area and good tuber’s quality. This experiment was carried out to select a cultivar of good tuber quality and to determine the ridge height for high-yielding. Methods and Results : It were investigated the characteristics of roots and tubers depending on different ridge heights in four varieties of Liriope platyphylla Wang et. Tang. There was not significantly different in shoot growth by different ridge heights. The tubers distribution in less than 10cm soil depth at the surface was decreased about 6.6% in the 15cm ridge height compared to 5cm. So, the mean distributed position of tubers was deepened at higher ridge. The cultivars that tubers were mainly distributed in the 10cm depth from surface was Seoungsu and Cheongyang natives’s lines. The no. of tubers per a plant and the ratio of marketable tubers were increased about 13.2% and 20.1% in the 15cm ridge height compared to 5cm. Considering the tuber sizes and ratio of marketable tubers, Cheongshim and Maekmundong 1 has good quality than Seoungsu or Cheongyang natives’s lines. The tuber yield increased about 19.5% in the 15cm ridge height compared to the 5cm. So, high ridge was advantaged to increase the yield. Conclusion : From the above results, the quality and quantity of tubers of Liriope platyphylla Wang et. Tang was good in the higher ridge. We recommended Cheongshim cultivar because of the large tuber sizes and high ratio of marketable tubers.

Background : Liriope platyphylla Wang et. Tang is a species of Liliaceae and its tuber is used as medicinal herbs. This medicinal plant is mainly cultivated in Cheongyang and Buyeo of Chungcheongnam-Do and Miryang of Gyeongsangnam-Do. It is necessary to establish a cultivation techniques for stable production to solve the problems that are found on farms. These farm surveys were carried out to obtain the basic data on soil environment and cultivation conditions at main producing areas of Chungnam-Do in Liriope platyphylla Wang et. Tang. Methods and Results : The surveys were investigated from 37 farmers in Cheongyang and 32 farmers in Buyeo who cultivate Liriope platyphylla Wang et. Tang. There were slightly differences in the local characteristics between Cheongyang and Buyeo. The rate of successive cropping for more than 3 years were 19% at Cheongyang and 41% in Buyeo. The reason may be due to the a small-owned field. It has grown 28% at Cheongyang and 59% at Buyeo in paddy fields. The injury by successive cropping that farmers argued were the increasing pest/disease occurrence, degradation of tuber quality and yield reduction. Crops planted after the harvest of L. platyphylla were mostly Pepper or soybean in upland. It is necessary to reduce the injury of successive cropping. The results of 112 soil analysis data were classified by 8 soil series. There are many acidic soil, lack of organic matter, excess phosphate. However, it did not analyzed the influences on the growth and yield of the crop as affected by different soil conditions. Conclusion : From the above results, it is necessary to develop cultivation techniques on the reduction of injury of successive cropping, such as cropping system, seedling transplanting cultivation, cultivation methods for good agricultural practices(GAP) and soil improvement.

Background : Usually, planting and harvest time is around the middle of April. A labor shortage deu to her planting time as harvest time and some farmers were late planting culture. After planting until take rooting It will take more than one month when late planting growth is bad. Seedling cultivation, just taking rooting is agricultural growth promotion. Methods and Results : So did design the optimum nursery days and number of plants. Nursery days were 30 days and 45 days and number of plants 1, 3, 5 plants. Plant length was longer in less number of plants while Root length was longer in more number of plants of Temporary planting seedling, Leaf number was a big increase but number of plants was not a big shift. In post-havest survey, plant length was longer in 3 number of stem and root length was longer in one number of stem. 45 days and 3 number of stem seedling was the most good in number, weight and length of tuberous root. Yield of 45 days and 3 number of stem seedling was higher 7% than 1 number of stem seedling and higher 5% than 5 number of stem seedling. 45 days and 3 number of stem seedling was 1,789 thousand per 10a in income was the most. Conclusion : Result of this study, for rasing healthy seeding nursery days 45 days and number of plants 3 plant

Background : Acreage of rain-shelter plastic greenhouse to prevent anthrax is being gradually increased according to growing importance of safe Boxthorn production. But When Boxthorn is grown in the hot season in rain-shelter plastic greenhouse, quantity of Boxthorn decreases. therefore the research was carried out to investigate Fertilization mode. Methods and Results : Chungwoon was very strong self-incompatibility. Chengyangjaerae, Chengyang18 and CBP11542-206 was self-compatibility. Artificial fertilization rate was slightly higher in roof and side vents than in side vents of rain-shelter plastic greenhouse in hot season. Pollen sterility due to high temperature is not critical because artificial fertilization rate was high in side vents of rain-shelter plastic greenhouse in hot season. Airborne fertilization rate was significantly lower in all varieties. Open fertilization rate was higher in roof and side vents than in side vents of rain-shelter plastic greenhouse Conclusion : Roof and side vents was good in compared to side vents in rain-shelter plastic greenhouse of Boxthorn because of high Airborne and open fertilization rate. Self-compatibility varieties were better than self-incompatibility varieties in rain- shelter plastic greenhouse because of high open fertilization rate.

Background : Fruit picking is the most labor consuming procedure for Boxthorn (Lycium chinense Mill.) growers. Recently fruit harvesters were invented and improved in the efficiency to fulfill the expectation of farmers through field demonstration tests. Additionally, T-type hedge cultivation method harmonized well with fruit picking machine, but it also needed to be improved in its’ pruning methods for the efficiency of fruit collecting procedure. Methods and Results : In this experiment, ‘Cheong-un’ cultivar and local variety were grown in the rain shelter greenhouses for the tests. Plots were set by randomized block design. There were two types of previously developed harvesters. One vibrates it’s harvest rod right and left (A type) and the other shakes it’s rod back and forth (B type). Among two harvesters A type was superior to B type in efficiency and convenience in use and it was able to harvest eleven times more fruits than manual picking in simple comparison test. In field comparison test, A type harvester performed 4.6 to 5 times more fruits than manual fruit picking. T-type hedge cultivation method is the proper way of enhancing efficiency of harvester. In the test, wiry perpendicular branches formed by once pruning method hindered moving forward of fruit collector. However installing collecting net with velcro along the edge under the field increased the efficiency and handiness of fruit harvester and well harmonized with T-type hedge cultivation. Conclusion : From the above results, the newly developed fruit harvester could increase the annual income of Boxthorn growers by reducing fruit picking labor and time.

Background : Boxthorn has been cultivated by conventional fertilization and irrigation methods that can be characterized by applying a great deal of fertilizers and a small amount of water compared to the other crops. With increasing rain shelter greenhouses, there were significant needs for developing new fertigation and irrigation methods. In this study, several prescribed fertigation methods for balanced fertilizer application and a few irrigation start methods based on soil moisture tension has been tested to confirm the fruit quality and productivity for Boxthorn growers. Methods and Results : In the experiment, ‘Cheong-un’ cultivar was grown in the rain shelter greenhouses for the tests. Plots were set by randomized block design. According to the growth investigation on the ‘Cheong-un’ cultivar which is supplied with four different formulated fertigation, the formulation N 15 me/ℓ increased the size and weight of fruit leading to 17% increased yield than the others. Additionally, on the amount of annual supply of fertigation with the concentration of N 15 me/ℓ, the treatment applied with 10kg/10a during the cultivation period facilitated overall growth of ‘Cheong-un’ cultivar and increased the yield as well. In the irrigation experiment ‘Cheong-un’ cultivar was grown in the four differently planned treatments that start irrigation at –20, -40, -50 and -100kPa soil moisture tensions. According to the investigation, the irrigation treatment starting at -50kPa enhanced the number of fruit bearing and productivity of Boxthorn consistently during two years. Conclusion : Therefore, the newly developed fertigation and irrigation methods could enhance the annual income of Boxthorn growers by increasing growth and yield.

Background : The effect of ridge-up bed cultivation and stem training method on the plant growth and yield of Cynanachum willfordii was investigated. Methods and Results : Domestic variety of Cynanachum willfordii was tested at Chungbuk ARES under the following conditions. Nonsupporting (custom) and I-form supporting was used for stem training method, and ridge height was set as 30 (custom) and 60 cm (high ridge). Planting spacing was adjusted to 30 (interrow spacing) × 20 cm (intrarow spacing), and other major management was followed the method of standard cultivation for medicinal crop. Investigated result from leaf characteristics, leaf length was longer in high ridge cultivation (HRC) as 11.1 cm than custom cultivation (CC) as 10.6 cm. Leaf width is proved to be 12.8 (HRC) > 11.2 cm (CC). Leaf number is proved to be 294 (HRC) > 254/plant (CC), with higher number of 44/plant at HRC and weight/10 leaves were more heavy at HRC (4.9 g) than that of CC (2.6 g). It was more fruit setting at HRC over 15/plant. According to the stem training method and ridge height from nonsupporting cultivation, main root was 4.0 (CC), higher than that of HRC over 0.5/plant. However, root length was more longer in HRC (28.6) than that of CC (25.0 cm). Main root diameter was also showed more thicker pattern in HRC. From staking cultivation, root number of HRC was 7/plant, it was recorded more 3/plant than that of CC, and it was also same pattern in main root length and root diameter. By the standard of commercial root, yield of living roots in nonsupporting cultivation were 59.0g/plant (HRC), it was more heavy over 10.4g/plant than that of CC. In staking cultivation, HRC were recorded as 74.2g/plant, more heavy 6.9g/plant than that of CC. Yield from I-form support stem training and ridge-up bed cultivation of HRC was higher approxmiately 52.7% than that of CC. Conclusion : Overall growth by high ridge cultivation of I-form support was good and yield of HRC was also increased over 52.7% than that of CC.