본 연구는 불법적으로 수산물에 사용될 수 있는 염료 18종에 대한 안전관리 강화를 위해 정량 및 정성 분석이 가능한 LC-MS/MS를 적용하여 검증하기 위해 수행되었다 . 확립된 시험법은 CODEX CAC/GL-71 가이드라인에 따라 직선성, 정밀성 , 정량한계 및 회수율 등을 통해 유효성을 확인하였다 . 대상시료에 1% 아세트산을 함유한 아세토니트릴로 추출 후 C18 과 PSA로 정제하였다 . 본 실험에서 정량한계는 0.002 mg/kg 수준으로 정량한계를 포함한 농도에 따라 검량선을 작성하였고 모두 0.98 이상의 직선성을 확인하였다 . 또한 정확성은 63%-112% 이고, 정밀도는 15% 이하로 재현성이 우수하였다 . 국내 유통 중인 수산물 124 건을 수거하여 개발된 분석법의 적용성 검증과 안전성을 확인하고자 잔류실태조사를 실시 하였고 그 결과 7건이 미량으로 검출 되었고 부적합은 없었다 . 확립된 시험법은 수산물 안전관리에 활용할 수 있을 것으로 사료되는 바이다 .

We report an analysis of two poorly studied eclipsing binary stars, GSC 04396-00605 and GSC 04395-00485 (recently named V455 Dra and V454 Dra, respectively). Photometric data of the two stars were obtained using the 1-m Korean telescope of the LOAO operated by KASI while monitoring the cataclysmic variable DO Dra in the frame of the Inter-Longitude Astronomy (ILA) project. We derived periods of 0.434914 and 0.376833 days as well as initial epochs JD 2456480.04281 and JD 2456479.0523, respectively, more accurate than previously published values by factors 9 and 6. The phenomenological characteristics of the mean light curves were determined using the New Algol Variable (NAV) algorithm. The individual times of maxima/minima (ToM) were determined using the newly developed software MAVKA, which outputs accurate parameters using "asymptotic parabola" approximations. The light curves were approximated using phenomenological and physical models. In the NAV algorithm, the phenomenological parameters are well determined. We derived physical parameters using the Wilson-Devinney model. In this model, the best-fit parameters are highly correlated, thus some of them were fixed to reasonable values. For both systems, we find evidence for the presence of a cool spot and estimate its parameters. Both systems can be classified as overcontact binaries of EW type.

PURPOSES : Asphalt pavements are damaged by various causes, such as cracks, potholes due to climate, and traffic environment. Asphalt aging has a significant effect on cracks, which may also form potholes. The purposes of this study are to estimate the change in asphalt binder and mixture performance and to correlate the physicochemical changes of the asphalt binder and mixture performance with aging. METHODS : The aging process of the asphalt was determined in the laboratory, based on the methods used in previous studies. In terms of consistency (stiffness), crack-resistance performance, moisture susceptibility, SK investigated the binder part, and Shoreki investigated the mixture part depending on the aging time. RESULTS : The consistency (stiffness) and brittleness of both the asphalt binder and mixture tended to increase with aging. In particular, the crack-resistance performance of the asphalt binder (G*sinδ, ductility, and ΔTc) and mixture (flexural fatigue test and Cantabro test) deteriorated because of asphalt aging. Furthermore, the aging mechanisms (oxidation and polymerization) were identified based on the chemical structure analysis. CONCLUSIONS : It is confirmed that the aging affected the chemical composition change and the physical properties of the asphalt. Asphalt pavements are significantly affected by the aging characteristics of the binder. It is concluded that the crack-resistance performance of the mixture decreases with aging due to these physical and chemical changes.

Background: The foot drop stimulator is designed to improve the walking ability of foot drop in patients after stroke, however, studies on clinical effects are still lacking. Objective: To investigate the effect of a foot drop stimulator on the walking and balancing abilities of foot drop patients after a stroke. Design: One-Group (Pretest-Posttest) Design. Methods: All subjects walked in all three conditions: foot drop stimulator (FDS) ankle foot orthosis (AFO) and barefoot. Primary outcome measures were assessed for walking and balance using a 10-m walking test (10MWT) and a timed up and go test (TUG). Secondary outcome measures consisted of a brief user interview, and the patients recorded the advantages and disadvantages of each condition. Results: FDS, AFO, and barefoot conditions showed a statistically significant difference in 10MWT and TUG (P<.001) as a result of comparing three conditions. FDS and AFO were significantly different from the barefoot condition as post-hoc results; however, there was no significant difference between the two conditions (P>.05). Conclusion: In this study, the foot drop stimulator contributed to improving the balance ability, and the walking ability was similar to the effect of the anklefoot orthosis.

Background: Ankle instability usually occurs after stroke, and contributes to unsafe walking and associated risk of falling in the affected patients. Objective: To investigate the effects of kinesiology taping (KT) on gait and balance ability (center of pressure, CoP) in patients with ankle instability after stroke. Design: One group, pre-post design. Methods: A total of 11 patients with ankle instability after stroke were enrolled. In all subjects, the gait and balance ability were assessed under 2 conditions: KT and barefoot. Gait and balance ability was assessed using GAITRite system and FDM-S platform. Results: Comparison between KT and barefoot condition, KT condition was significantly higher in velocity, cadence, step length, and stride length than barefoot condition (P<.05). KT condition was significantly lower in CoP path length and sway speed than barefoot condition (P<.05). Conclusion: KT indicated potential as a helpful method for walking and balancing ability in patients with ankle instability after stroke. Therefore, this study recommends KT as an option applicable to the stroke with ankle instability.

As the population of consumers of outdoor wear expands, there is increasing interest among consumers in trendy lifestyle outdoor. Accordingly, it is essential to develop designs that are drawn from outdoor wear. This can be limited to traditional mountaineering suits, and designs developed from men’s outdoor zip-up T-shirts, which are highly utilized in everyday life, are very important. This study developed the design of a summer zip-up T-shirt for men with both functional and aesthetic qualities. Visual images of foreign brands and domestic outdoor-focused T-shirts from the spring/summer seasons of 2013-2019 were collected to analyze the following modeling elements: silhouettes, details, colors, materials, and prints. In addition, the design concept was derived by analyzing presentations from the global outdoor trade Fairs (2013-2019). To develop a sustainable eco-friendly outdoor summer T-shirt for men, the concept was derived according to three categories: eco-friendly, sportismexpressed active elements of sports, and an outro-concept of urban town wear as an outdoor lifestyle. Thus, a total of 12 styles of design were developed. High utilization of the outdoor wear T-shirts (which are also popular as lifestyle wear), is an important aspect of building a segmented lineup of the slow-growing outdoor wear market and supports expansion of the scope of theoretical research on outdoor wear design.

Many companies make considerable efforts in implementing supply chain management systems to increase their competitiveness. In particular, there are significant investments in their ICT (Information and Communication Technology) and supply chain. However, considerable diversity exists in how well firms have been able to assimilate ICT and leverage the business value of it. In addition, studies on supply chains, particularly Real Time Enterprise (RTE) competence, which is cited for rapid information sharing and dynamic capabilities for each role in the supply chain, are insufficient. The purpose of this paper is to explore and evaluate effect of ICT utilization capabilities, including ‘ICT internal cooperative capabilities’ and ‘ICT operational flexibility capabilities’, on RTE capabilities (flexibility, visibility and prediction) and supply chain performance. To validate the research model proposed in the study, survey was conducted on companies using ICT along the supply chain of manufacturing industries. 216 data were used, SPSS and AMOS were used for the analysis methods. Study results showed that ICT internal collaboration capabilities affect agility, one of RTE capabilities, but not visibility and prediction, and ICT operational flexibility capabilities have affected all three RTE capacities. And, RTE capabilities had a huge impact on supply chain performance as expected. In this paper, it has been found that the ICT capabilities in manufacturing are an important factor in improving RTE capabilities that are important in the supply chain and improving the performance of the supply chain.

Nitric oxide (NO)-induced protein S-nitrosylation triggers mitochondrial dysfunction and was related to cell senescence. However, the exact mechanism of these damages is not clear. In the present study, to investigate the relationship between in vitro aging and NO-induced protein S-nitrosylation, oocytes were treated with sodium nitroprusside dihydrate (SNP), and the resultant S-nitrosylated proteins were detected through biotin-switch assay. The results showed that levels of protein S-nitroso thiols (SNO)s and expression of S-nitrosoglutathione reductase (GSNOR) increased, while activity and function of mitochondria were impaired during oocyte aging. Addition of SNP, a NO donor, to the oocyte culture led to accelerated oocyte aging, increased mitochondrial dysfunction and damage, apoptosis, ATP deficiency, and enhanced ROS production. These results suggested that the increased NO signal during oocyte aging in vitro, accelerated oocyte degradation due to increased protein S-nitrosylation, and ROS-related redox signaling.

The establishment of porcine embryonic stem cells (ESCs) from porcine somatic cell nuclear transfer (SCNT) blastocysts is influenced by in vitro culture day of porcine reconstructed embryo and feeder cell type. Therefore, the objective of the present study was to determine the optimal in vitro culture period for reconstructed porcine SCNT embryos and mouse embryonic fibroblast (MEF) feeder cell type for enhancing colony formation efficiency from the inner cell mass (ICM) of porcine SCNT blastocysts and their outgrowth. As the results, porcine SCNT blastocysts produced through in vitro culture of the reconstructed embryos for 8 days showed significantly increased efficiency in the formation of colonies, compared to those for 7 days. Moreover, MEF feeder cells derived from outbred ICR mice showed numerically the highest efficiency of colony formation in blastocysts produced through in vitro culture of porcine SCNT embryos for 8 days and porcine ESCs with typical ESC morphology were maintained more successfully over Passage 2 on outbred ICR mice-derived MEF feeder cells than on MEF feeder cells derived from inbred C57BL/6 and hybrid B6CBAF1 mice. Overall, the harmonization of porcine SCNT blastocysts produced through in vitro culture of the reconstructed embryos for 8 days and MEF feeder cells derived from outbred ICR mice will greatly contribute to the successful establishment of ESCs derived from porcine SCNT blastocysts.

한국산 개미과 비늘개미속의 2미기록종인 Strumigenys alecto과 Strumigenys solifontis가 분포함을 보고한다. 2 종의 일개미에 대한 형질과 사진을 제공한다.

The objective of this research work was to know ovarian dynamics and pregnancy rate of cyclic Murrah buffalo cows with induced estrus by administration of prostaglandin F2α (PGF2α) and timed artificial insemination (TAI) with frozen thawed semen. A total of 31 female buffaloes were selected for the study. The buffalos having matured CL observed by ultrasonography were given one intra muscular injection of cloprostenol 500 μg and TAI was performed using frozen thawed semen of Indian Murrah buffalo bull. Results showed that 90.32% (significantly, at p < 0.05) cows explore the sign of heat after injection of PG and 67.85% (significantly, at p < 0.05) cows were become pregnant out of 28 inseminated (TAI) cows. In the 28 inseminated (TAI) cows, average number of smaller and larger size of follicles were non-significantly (p > 0.05) higher at day 3 post PG injection, but the medium size of follicles was non-significantly (p > 0.05) higher at PG injection. At day 3 post PG injection the diameter of follicles was significantly (p < 0.05) higher, but the diameter of CL was significantly (p < 0.01) lower compared at PG injection. At PG injection the diameter of largest follicle was non-significantly differences (p > 0.05) in between pregnant and non-pregnant cows. But at day 3 post PG injection it was significantly (p < 0.01) higher in pregnant cows compared to non-pregnant cows. The number of small, medium, and large follicles at PG injection and at day 3 post PG injection were non-significantly (p > 0.05) difference in between pregnant and non-pregnant buffalo cows. Finally, it is concluded that the CL was effectively regresses and induced the sign of heat in buffalo cows and after AI the cows were become pregnant with significant rate. The study will help to the veterinarian and researcher to know the efficacy of PG injection and AI for reproductive efficiency in buffalo cows.

As a preclinical study, many researchers have been attempted to convert the porcine PSCs into several differentiated cells with transplantation of the differentiated cells into the pigs. Here, we attempted to derive neuronal progenitor cells from pig embryonic germ cells (EGCs). As a result, neuronal progenitor cells could be derived directly from pig embryonic germ cells through the serum-free floating culture of EB-like aggregates (SFEB) method. Treating retinoic acid was more efficient for inducing neuronal lineages from EGCs rather than inhibiting SMAD signaling. The differentiated cells expressed neuronal markers such as PAX6, NESTIN, and SOX1 as determined by qRT-PCR and immunostaining. These data indicated that pig EGCs could provide valid models for human therapy. Finally, it is suggested that developing transgenic pig for disease models as well as differentiation methods will provide basic preclinical data for human regenerative medicine and lead to the success of stem cell therapy.

In this study, we investigated whether infusion of colorectal cancer cell line and PMSG could increase endometrial cancer. As a result, our study confirmed that the injection of colorectal cancer can cause inflammation and cancer in the uterus and increase the VEGF gene in the uterus. The study also found that endometrial cancer was associated with PMSG.

Enamel knot (EK)—a signaling center—refers to a transient morphological structure comprising epithelial tissue. EK is believed to regulate tooth development in early organogenesis without its own cellular alterations, including proliferation and differentiation. EKs show a very simple but conserved structure and share functions with teeth of recently evolved vertebrates, suggesting conserved signaling in certain organs, such as functional teeth, through the course of evolution. In this study, we examined the expression patterns of key EK-specific genes including Dusp26 , Fat4, Meis2, Sln , and Zpld1 during mice embryogenesis. Expression patterns of these genes may reveal putative differentiation mechanisms underlying tooth morphogenesis.

Purpose of the study is to explore the effect of the types of mobile coupons(textvs. image-focused coupons; free-gift vs. discount coupons) on characteristic perception of mobile coupons, and the causal relationships among characteristic perception, attitude, and use intention of mobile coupons. A total of 140 university students participated in experiments with questionnaires including one of the four stimuli. Important findings are as follows. First, image-focused mobile coupons generated more enjoyment than did text-focused coupons. However, the text/image-focused coupons were not different in perception of informativeness and credibility of mobile coupons. Second, enjoyment perception was significantly increased when image-focused contents were combined with discount coupons whereas enjoyment perception was decreased when text-focused contents were combined with free-gift coupons. This interaction effect reflects that the level of enjoyment of consumers can be changed in terms of the combination of the value-provision types of coupons and the text-image focused contents. Third, it was found that consumer perception of coupon characteristics formed attitudes toward mobile coupons, and use intention of mobile coupons was determined by attitudes toward mobile coupons. Study findings may fill the void of research investigating the effect of text-image contents and the types of coupons on consumer reponses toward mobile coupons. Mobile coupons have limited quantity of information within a small size of mobile phone screen, therefore, the results were not consistent with prior research tested with mobile advertisements indicating the effect of text-image contents on perception of informativeness and credibility.

본 연구는 관상가치가 뛰어난 산꼬리풀의 분화 재배법을 확립하고자 차광, 기비, 추비, 왜화제 및 적심처리를 각각 달리하여 수행하였다. 연구의 결과 차광 시 산꼬리풀의 생육이 감소 하였으며, 차광률이 증가할수록 크게 억제되었다. 기비 처리는 초장, 절간장, 경직경 및 엽수를 유의적으로 증가하였으나, Hyponex를 이용한 추비 처리는 무처리와 비교했을 때 생육증가에 큰 영향을 미치지 않았다. 왜화제 처리시 diniconazole은 농도에 관계없이 왜화효과가 나타났으며, 농도가 증가할수록 크게 억제되었다. Daminozide는 저농도 처리시 무처리와 비교하여 큰 차이가 없었으나, 2000mg·L-1 농도로 처리하였을 때 왜화효과가 나타났으며, 개화율은 diniconazole 저농도에서 가장 낮은 결과를 보였다. 한편 적심 처리에 따른 유의적인 생육 차이는 확인되지 않았다.

This study investigated the efficacy of four Brucella (B.) abortus recombinant proteins, namely adenylate kinase (Adk), nucleoside diphosphate kinase (Ndk), 50S ribosomal protein (L7/L12) and preprotein translocase subunit (SecB), as a combined subunit vaccine (CSV) against B. abortus infection in BALB/c mice. Immunoblotting assay showed that these four recombinant proteins as well as pcold-TF vector reacted individually with Brucella-positive serum, but not with Brucella-negative serum. The peripheral blood CD4+ T cell population was increased in CSV-immunized mice compared to PBS and pcold-TF vector groups. In addition, CSV and pcold-TF groups displayed induced IgG1 and IgG2a antibodies production compared to PBS and RB51 group, whereas IgG2a titer was higher than IgG1 titer in CSV group. The secretion profiles of IgG1 and IgG2a production together with an enhancement of CD4+ T cell population suggested that CSV did not only induce T helper 1 (Th1) T cell immunity but also humoral immunity. Therein, Th1 T cell immunity is more predominant in eliminating intracellular bacteria B. abortus. Furthermore, CSV immunization significantly reduced the bacterial burden in the spleen as well as the spleen weight in comparison to PBS and pcold-TF groups. Altogether, combination of these antigens could be potential to induce protective immunity against B. abortus infection in animals.

The purpose of this study was to examine the moderating effect of board education diversity on the relationship between ownership concentration and environmental accounting disclosure. The study was driven by stakeholder’s theory. The longitudinal research design was adopted in the study. The study targeted 27 listed firms from 2008 to 2017. Panel regression analysis results indicated ownership concentration (β = -.131, ρ<.05) had a negative and significant effect on environmental disclosure in Kenyan firms. However, Board education diversity positively moderated the relationship between ownership concentration (β=.138, ρ<.05) and environmental accounting disclosure. Thus, board education diversity is an enhancing moderator in the relationship between ownership concentration and environmental accounting disclosure. The findings validate stakeholder theory's proposition. The study recommends that firms listed in the NSE ought to diffuse ownership concentration, and their boards should be well educated and experienced to enhance environmental accounting disclosure.

국내 미기록종인 Saridoscelis sphenias Meyrick을 보고한다. 이 종은 상제집나방아과의 두번째 종으로 기록된다. 정확한 동정을 위해 외부 형질과 수컷 생식기를 그림과 함께 기술한다. Saridoscelis sphenias Meyrick은 블루베리의 잠재 해충으로 국내 농가에 주의를 요한다.

Royal jelly (RJ) is a gelatinous substance that bees produce to feed bees and queen bees. It’s frequently sold as a dietary supplement to treat a variety of physical ailments and chronic diseases. While it has long been used in traditional medicine, its applications in Western medicine remain controversial. The inhibitory effect of royal jelly on osteoarthritis was investigated in primary cultured rat cartilage cells and monosodium-iodoacetate (MIA)-induced arthritis rat model 10-hydroxy-2-decenoic acid (10-HAD) is the main fatty acid present in RJ. Among the criteria for RJ quality analysis, 10-HAD content has been proposed as a freshness parameter. We investigated the effect of RJ on the improvement of osteoarthritis on SD rats and they were divided into five groups. In this study, we examined the effect of enzymatic royal jelly (ERJ) administration on osteoarthritis. To determine the antiinflammatory effects of RJ, tumor necrosis factor alpha (TNF-α) and Interleukin-6 (IL-6) expression were measured after lipopolysaccharide (LPS) activation in RAW 264.7 cells. In in vivo animal study, osteoarthritis was induced by intra-articular injection of MIA into knee joints of rats. As a results, ERJ showed that TNF-α and IL-6 levels were decreased by ERJ treatment in a dosedependent manner. In conclusion, ERJ extract was able to inhibit articular cartilage degeneration by preventing extracellular matrix degradation and cartilage cell damage. It was considered that ERJ extract may be a potential therapeutic treatment for degenerative osteoarthritis.