국제교역의 발달로 외래병해충의 국내 침입 가능성이 높아지고 있으며, 기후 변화로 인해 토착화 가능성 또한 점차 증가하고 있다. 이에 대한 선제적인 연구를 위해 기존의 생물안전 연구 시설을 바탕으로 검역해충 실험동을 농림축산검역본부에 구축하였다. 본 시설은 검역해충에 대한 전문연구시설로써 내부해충의 외부유출을 방지하기 위해 실험실 입실시 풍속 25-35m/s 의 에어샤워 부스 통과시설과 각 연구실 출입문에 풍속 9-12m/s 의 샤워커튼이 설치되어 있으며, 자외선 해충유인등이 실험실 내외부 벽면에 장착되었다. 에어샤워 부스와 샤워커튼은 호박꽃과실파리. 벗초파리의 비행에 영향을 주어 실험실 외부로 유출하는 것을 방지할 수 있었다. 또한 이러한 연구를 바탕으로 실험실 표준운영 절차를 수립할 수 있었다.

Heamaphysalis longicornis is a major vector for Severe Fever with Thrombocytopenia Syndrome (SFTS) virus and the density of the vector has been increasing because of the climate change. The incidence of fatalities due to SFTS is increasing every year. In this study, to evaluate the SFTS transmission by ticks, the density of ticks mediating SFTS was monitored. Tick was collected every month from four different sites (Grass land, Mountain path, Grave, Copse) in Andong with the traps containing dry ice as CO2 attractants. Among 2,572 ticks of 3 species; H. longicornis, H. flava, and Ixodes nipponensis were most abundantly collected from April to August. H. longicornis is the richest species (92.8%), whereas Ixodes nipponensis was the least species (0.8%). The 54.5% of the sample were nymph stage and female/male ratio was 64.3%. According to the pathogene analysis, SFTS virus was detected from H. longicornis adult and larvae stages collected in July and August. In July samples, SFTS virus was detected only from grassland site, but the virus was observed in the sample from all four sites in August. For effective prevention of SFTS, the tick density should be continuously monitored based on the onset time of SFTS with the consideration of habitate, habit & life history of ticks.

The mandible character of the Lucanidae is vary by considering of developmental difference in male individual specimen. Especially, species of Genus Cyclommatus Parry, 1862 presents strong mandibular allometry. In this research, The species group which related to Cyclommatus montanellus Möllenkamp, 1904 has been evaluated for the first time in this research with inner teeth composition and positioning to establish clear taxonomic key to each species.

상주는 귀농 도시이자 농업 중심 도시로써 농업기술원을 유치할 만큼 생태학적 연구가치가 있는 친환경 도시이다. 특히 경천섬은 낙동강 본류에 있는 상주보 인근에 위치하고 있어 낙동강 생태 환경 연구의 중요 지역 중 하나이다. 하지만, 아직 경천섬에 대한 생물다양성 연구가 부족한 상태이다. 섬과 산지의 생태환경이 다르기 때문에 생물의 다양성도 다를 것이라 간주하고, 경천섬 주변의 생태학적 가치를 재조명할 기초자료를 조사하고자 한다. 이에 경천섬과 인근에 위치한 청룡사 길목 산지에 서식하는 곤충 채집을 통해 생물다양성을 조사하여 비교분석하는 것을 목적으로 한다. 채집방법은 직접잡기, 쓸어잡기, 함정트랩, 흡충관을 이용하였으며, 함정트랩에 사용된 유인제는 소주1.8ℓ와 빙초산350㎖를 혼합하여 사용하였다. 2018년 4월~8월 중 3회 채집과정을 수행하여 목표개체수를 확보하였고, 채집된 곤충은 에탄올을 채운 conical tube에 넣어 냉동실에 보관하여 보존성을 높였다. 채집과정에서 잡은 곤충들을 건조시키고 유사종끼리 분류하고 동정하는 과정을 거쳐 다양도지수, 우점도지수, 종풍부도지수, 종균등도지수, 유사도지수를 계산할 것이다. 기존에 알려지지 않았던 경천섬 일대의 생물다양성을 세세하게 분석하는 과정을 통해 경천섬의 생태학적 가치를 드러내고, 경천섬 일대의 자연환경관리와 보존에 필요한 정보나 체계적인 관리체계를 구축할 기초자료를 제공하고자 한다.

The objective of this study was to evaluate the effect of treating dairy cattle with exogenous human chorionic gonadotrophin (hCG), five (5) days post artificial insemination (AI) on serum progesterone (P4) concentration and pregnancy rate. In this experiment, five days after AI, cows were assigned randomly to two groups namely: a) treated group (67) which were administrered with 1500 IU hCG (Chorulon) and b) control group (61), which received no treatment. On day 5, 10, 15 and 20 after the artificial insemination, blood samples from a total of 8 cows (4 from each group) were collected and were analyzed for serum P4 concentration. Cows were detected for estrus according to standing heat by visual observation. Cows that were detected still in estrus after days 18-24 were re-inseminated and recorded as not pregnant (open). Pregnancy diagnosis was conducted by ultrasonographic examination and transrectal palpation of the uterus on approximately 60 days in cows that observed to be not in estrus. The conception rate in hCG treated and control groups were 52.5 and 36.1%, respectively. The results proved that there were no significant differences in conception rate between two groups (p=0.0568). However, pregnancy rates were reduced by hCG treatment. Average serum P4 concentrations did not differ between Hcg-treated and control groups on day 5 (0.377 versus 0.375 ng/ml). On day 20 serum P4 concentrations were greater in the treated group compared with the control group (3.085 versus 2.010 ng/ml). The treatment with hCG seemed to increase P4 level compared with the control. In conclusion, the results of this study showed that 1500 IU of hCG administered on 5 day post AI increased conception rate in dairy cows. This was supported by the results on serum P4 concentration which was greater in hCG treated group.

아케이드 슈팅게임은 과거 1970년대 후반부터 1990년 후반까지 대중적으로 큰 인기를 끌며 게임시장을 주도했다. 하지만 2000년대 이후 많은 PC와 콘솔의 보급으로 아케이드 게임이 급속도로 쇠퇴하기 시작했다. 하지만 최근 국내에서 하락세를 보였던 아케이드 게임이 크게 상승세를 보였고 앞으로도 점점 상승할 가능성이 높아 보인다. 따라서 본 논문은 아케이드 게임의 특징인 빠른 손동작을 최신 기술과 접목시켜 새로운 입력 방식을 개발함으로 더 높은 효용성을 끌어내어 검증 한다. 기존에 슈팅게임에서의 제스처 인터페이스 사용은 이미 그 효용성이 검증되었다. 다만 좀 더 복잡한 완성형 게임에서의 적용하기엔 미흡한 부분들이 있다. 이에 슈팅게임들의 요소를 좀 더 세분화하여 분석 후 각 요소들을 제스처에 매핑하였다. 기존 게임에선 조이스틱을 손으로 흔들며 진행하는게 일반적인 방식으로 본 게임에선 사용자가 직접 손을 상하좌우로 움직여 조작한다. 또한 진행에 필요한 스킬과 이벤트들을 각각의 매핑 된 제스처 로 인터랙션 한다. 개발된 인터페이스는 슈팅게임 'Cure'에 적용하여 G-Start, HCI creative Award 등에서 전시되었다.

본 논문은 3차원 축대칭 몰수체에 대해 소스 코드가 공개된 OpenFOAM 4.0을 이용하여 첫 번째 격자의 높이와 레이놀즈 수에 따른 마찰저항 변화에 대해 연구하였다. 마찰저항 계산을 위해 경계조건, 수치조건을 정립하였다. 축대칭 물체의 3차원 효과로 인해 거칠 기가 매우 작은 12 μΜ에서도 부드러운 표면과 비교해 마찰저항이 다르게 계산되었다. 레이놀즈 수가 커질수록 경계층의 두께 증가가 감소되었으며 이로 인해 마찰저항의 증가량이 감소되었다. 첫 번째 격자의 크기인 y+에 대한 영향에 대해서도 검토하였다. 첫 번째 격자가 log layer에 위치하고 있지 않으면 마찰저항과 표면의 전단력이 과도하게 예측되는 것을 확인하였다. 이는 경계층이 두껍게 예측되어 난류 에너지가 과도하게 예측되었기 때문으로 판단된다. 표면의 거칠기가 커질수록 경계층이 두꺼워지고 표면의 난류에너지가 증가되는 것을 확인하였다. 마찰저항을 정확하게 예측하기 위해서는 y+ 값, 거칠기 및 벽함수가 적절한 영역에 위치해야 함을 알 수 있었다.

The current protocols of methyl bromide (MeBr) fumigation on imported nursery plants, which are seriously infested with quarantine mealy bugs and scales, could be reevaluated because there were no limited uses of loading ratio, packing types etc. Another emerging issues is that the workplace health issues on MeBr fumigation are related with poor ventilation etc as well as well-known phytotoxic damages on them. In previous report, we have evaluated the efficacies of ethyl formate (EF) and phosphine (PH3) on different types of mealybugs. Herein, the phytotoxic damages of EF and PH3 on Alocasia, Sansevieria, Cactaceae and Croton genera were evaluated in terms of chlorophyll contents, color changes etc. This study showed that EF and PH3 fumigation on imported plants could be applicable to replacement of MeBr with further confirmation trials.

Methyl bromide (MeBr) is well-known as a hazardous chemical to exposed fumigators and related workers as well as a ozone depletion chemical. According to recent report in USA and Korea, when MeBr fumigated on orange and grape at low temperature (<10℃), absorbed MeBr on fruits was slowly released to workplace. To replace MeBr practices urgently, ethyl formate (EF) fumigation is considered safer to worker (TLV of EF = 100 ppm compared to MeBr's = 1 ppm) and free from residual problem. We reports confirmative liquid EF (FumateTM) with N2 trials, which is cost-effective and non-CO2 gas emitted, on imported grapes. The 4hr-fumigation of 70 gm-3of EF (CTP > 114 gh-3) in scale-up chamber (10m3) fumigation (20% l.r.), showed efficacies to many kinds of insect pests, non-phytotoxic damage, providing safer guideline at workplace.

애기유리나방의 성페로몬인 Z,Z-3,13-octadecadien-1-ol (Z3,Z13-18:OH)을 이용한 교미교란 효과를 검증하기 위하여 경남(진주)과 전남(순천)의 단감원에서 2016년부터 2년간 실험을 하였다. 2016년에는 순천과 진주A, 진주B의 세 단감원에 성페로몬 5 ㎎/septum을 각 나무마다 한 개씩 처리하여 교미교란 효과를 확인하였다. 그 결과, 순천과 진주A 과수원에서는 100 %의 교미교란 효과를 나타내었지만 진주B 과수원에서는 효과가 전혀 없었다. 2015년과 2016년 같은 기간에 애기유리나방의 밀도를 조사 결과 진주A 과수원에 비해 진주B 과수원의 애기유리나방 밀도가 약 6.7배 많은 것을 확인 할 수 있었다. 그리하여 2017년 제1화기 때에 진주B 과수원에서 성페로몬 량을 10 ㎎/septum로 늘려서 실험한 결과 교미교란 효과가 66.7%로 낮았다. 2017 2화기 때에 다시 진주A 과수원으로 장소를 옮겨 실험한 결과 100 %의 교미교란 효과가 있는 것을 확인하였다. 이러한 결과로 보아 교미교란 효과는 페로몬 량 뿐 아니라 지형의 영향도 받는 것으로 판단된다.

Azuki bean beetle (ABB), Callosobruchus chinensis (L.) is a field-to-storage pest of legumes. It is ideal to estimate its field population before it invades the stored legume stuffs. The seasonal occurrence was monitored with rocket traps supplied with synthetic female sex pheromone and with sweep netting during 2015-2016. Harvest damage was evaluated by taking purposive samples of the pods at harvest. Over-wintering stage was identified from the pod samples of left over plants. The results ascertained that October was the peak season of ABB in the azuki bean field. Grain damage and weight loss of the harvested azuki bean were 2.7% and 2.4% respectively, which could be the inoculum for storage loss. The adult emergence only from live pupae collected from pods revealed for its most probable over-wintering stage. In all, this study provides insight into some field ecological parameters of ABB that could be helpful in adopting its management strategies.

Plant essential oils (EOs) exhibit an array of biological activities against insect pests. However, their negative influences on the pheromonal activity of azuki bean beetle (ABB), Callosobruchus chinensis L., the field-to-storage pest of legumes, have not received research attentions. We evaluated the effects of three EOs and their two major components on the attractiveness of male ABBS to synthetic homofarnesal (2E- : 2Z-homofarnesal = 6:4) using Y-tube olfactometry in laboratory and rocket traps in the semi-open vinyl house. The tested EOs of Illicium verum, Croton anisatum and Gaultheria fragrantissima as well as the major components, trans-anethole and methyl salicylate ascertained significant negative effect against homofarnesal both in Y-tube olfactometry and in semi-open vinyl house tracing out a new opportunity of integrating them in ABB management programs both in field and storage.

This study aims to gather precise information on the real fabric color and texture, and purchasing intention of mobile shoppers buying clothes. Eighty volunteers participated in the sensory test on three smartphones with four colors and two fabricssmooth taffeta and hairy doeskin. This study carried out the posteriori test using the one-way ANOVA and Duncan test by SPSS21.0. In the analysis’ results of color preference, there were no differences among the four colors of taffeta between the smartphones, but different preferences between the red and yellow doeskin exist. In the case of the Samsung phone, which has an immense color distortion, the red fabric has a low color preference. In contrast, on the Apple phone yellow fabric had the highest preference because of its brightness. The Apple phone also has the highest purchasing intention of yellow colored taffeta, which is similar to the color preference results, although the real fabric has the opposite result. For doeskin, the real red and blue colored fabric has the highest purchasing intention. The Samsung phone has the biggest color mismatch with the real fabric. It also has the lowest purchasing intention of red taffeta fabric, while the LG phone has the lowest purchasing intention of blue fabric. Using the paired comparison method of the similarity between ‘real’ fabrics and the mobile version of fabric colors has a low similarity on all four colors of taffeta and doeskin fabrics. Therefore it can be concluded that phones do not represent the ‘real’ fabric color.

Alpha-linolenic acid (ALA) is one of n-3 polyunsaturated fatty acids and found mainly in the chloroplasts. Many studies have been reported that intracellular reactive oxygen species (ROS) in mammalian oocytes were reduced by supplementation of ALA in in vitro maturation (IVM) medium. Based on these reports, we expected that ALA acts as an antioxidant during IVM of porcine oocytes. Therefore, the objective of this study was to investigate the antioxidant effect of ALA supplementation during IVM in porcine oocytes. The cumulus-oocyte complexes (COCs) were incubated in IVM medium containing 200 μM H2O2 or H2O2 with 50 μM ALA for 44 h. Nuclear maturation stage of oocytes was evaluated using aceto-orcein method. For measurement of oxidative stress state, intracellular ROS and glutathione (GSH) levels were measured using carboxy-DCFDA and cell tracker red, respectively. In results, oocytes in metaphase-II (MII) stage development was significantly reduced in H2O2 group compared to non-treated control group (61.84±1.42% and 80.00%, respectively; p<0.05) and it was slightly recovered by treatment of ALA (69.76±1.67%; p<0.05). The intracellular GSH levels was decreased in H2O2 groups compared with control groups, but it was enhanced by ALA treatment (p<0.05). On the contrary, H2O2 treatment increased intracellular ROS level in oocytes and H2O2-induced ROS was decreased by treatment of ALA (p<0.05). Our findings suggested that ALA treatment under oxidative stress condition improve oocyte maturation via elevated GSH and reduced ROS levels in oocytes. Therefore, these results suggest that ALA have an antioxidative ability and it could be used as antioxidant in in vitro production system of porcine embryo.