The prevalence of cancer in companion dogs is growing nowadays with the increasing worldwide population of domestic dogs. Since there is a less established standard of care in veterinary medicine, investigational treatments, such as the development of biomarkers can be considered as a therapeutic intervention for early diagnosis. Despite the enormous efforts that have been invested in the search of biomarkers, still, there is a need for easy detection of significant biological markers for predicting canine cancers at an early stage. In this study, we have analyzed the expression pattern of previously reported 46 canine cancer-associated candidate genes in blood specimens using real-time qPCR. We hypothesized that analysis of gene expression in blood would provide preliminary evidence of local or systemic immunogenic response which further contribute to the easy and early diagnosis of canine cancer from blood specimen as an analytical tool. The datasets included a total of 22 blood samples collected from different breeds of dogs diagnosed with cancer and five from healthy normal dogs. RT-qPCR analysis was performed by employing the SYBR Green PCR mix to assess the expression of these 46 genes in a total of 27 samples. From our result, a total of nine genes (ROS1, C1QA, CD48, IL1b, TLR2, IL2R, CHI3L1, CTSS, and TLR7) were found to be significantly up-regulated (p < 0.05 and p < 0.01) in the cancer samples compared to non-cancer samples. The relative expression level of ROS1, C1QA, CD48, IL1b, TLR2, IL2R, CHI3L1, CTSS, and TLR7 genes was 5.74, 4.78, 3.94, 2.94, 2.57, 2.53, 2.50, 2.04, and 2.57, respectively, in cancer samples compared to non-cancer samples. Thus, our results reveal several highly expressed cancer genes that can be therapeutic target genes for further testing in canine cancers.

연구는 참외 재배 지에서 흰가루병, 담배가루이 및 두점박이응애가 동시에 발생하였을 때 45, 40, 35°C (대조구)의 온도에서 측창으로 환기 처리 시, 온실 내 온 ․ 습도의 변화, 병충해 발생과 잎말림, 그리고 개화조절에 미 치는 효과를 검토하였다. 3월 3일 ‘히든파워’ 대목에 접붙여진 ‘알찬꿀’ 참외를 40cm 간격으로 격리상에 심었고, 위 에 언급한 병해충이 모든 처리구에서 발생한 6월 18일부터 7월 13일까지 처리하였다. 온실의 온도는 맑은 날에는 설정 온도 지점까지 증가되었고, 45°C 환기 처리에서 고온 고습이 약 9시간 동안 유지되었다. 주간 최고 기온과 최 저 상대습도 차이는 45°C 환기 처리에서 가장 높았다. 환기 처리 11일 후에는 흰가루병과 두점박이응애 피해가 45°C 환기 처리에서 거의 회복되었지만 40°C와 35°C에서는 그렇지 않았다. 처리 14일 후, 담배가루이와 두점박이 응애 밀도는 45°C에서 유의하게 감소하였으나 흰가루병 증상은 유의하게 감소하지는 않았다. 잎말림은 고온에서 유발되었으나 45°C에서도 심하지 않았다. 처리 26일 후, 새로 나온 줄기의 15 마디의 개화수를 조사한 결과, 45°C에 서 암꽃이 전혀 나오지 않았고 수꽃은 1.2개로 나타났다. 이상의 결과는, 고온기에 45°C의 고온에서 2－3주간 환기 처리는 온실 내부의 고온 고습을 유도하여 흰가루병, 담배가루이, 두점박이응애를 통제하고, 개화를 억제하여 참외 의 영양 생장을 회복할 수 있는 방법으로 사료되었다.

In order to extend the business viability of carbon nanotubes (CNTs), research on CNT dispersion in a solvent as well as in polymer matrix should be established. Herein, three kinds of dispersing agents, sodium deoxycholate (DOC), sodium dodecylbenzene sulfonate (NaDDBS), polyvinyl pyrrolidone (PVP), are selected and applied to quantify the dispersibility and dispersion stability of CNT aqueous dispersion. The dispersibility of CNT dispersion with the PVP, evaluated via viscosity and particle size analyses, are superior to those with the DOC and NaDDBS dispersing agents. CNT aqueous solution dispersed with PVP showed slightly higher viscosity and narrower particle size distribution than those with DOC and NaDDBS dispersing agents. In addition, the dispersion stability of CNT dispersion with the PVP, measured via lumisizer analyses, are superior to those with the DOC and NaDDBS dispersing agents. HR-TEM analysis verifies that the outstanding dispersibility and dispersion stability of CNTs in aqueous solution are due to the effect of the robust polymer wrapping of the PVP dispersing agent on the CNT surface. From the results of this study, the guidelines for the selection of the suitable dispersing agents and the systematic evaluation of dispersibility and dispersion stability of CNT dispersions can be suggested.

Because sows are industrially vital for swine production, monitoring for their health or disorder status is important to ensure high reproductive performance. Especially, ambient temperature changes in different season, especially during summer, are directly influenced to the reproductive performance of sows. Although the serum biochemical parameters are widely applied in the veterinary medicine with wide ranges for the physiological process, the values are also influenced by several factors such as age, breed, gender, and stress. In addition, domestic sows in Koreaspecific reference interval (RI) for serum biochemistry has not been established yet. Therefore, the present study was aimed to evaluate seasonal variation of RIs in the serum biochemistry in domestic sows in Korea at different seasons and to establish normal RIs using a RI finding program (Reference Value Advisor). Significant difference (p < 0.05) on the different seasons were identified in several serum biochemical parameters including BUN, CRE, GGT, GLU, ALB, TP, LDH and Na in sows. Therefore, we further established RIs, specific in domestic sows in Korea regardless of season. The established RIs based on the serum biochemical values provide a baseline for interpreting biochemical results in the domestic sows in Korea, regardless of seasonal effect. It may contribute to develop a strategy for better reproductive performance by improving breeding management practice and evaluating health of pig herds, which facilitate to avert the economic loss in summer infertility in sows.

A 13-year-old mixed dog was referred to the animal medical center, Gyeongsang National University. Lung masses were diagnosed at the left cranial and caudal lobes through diagnostic imaging, and consequently, left pneumonectomy was performed using a self-cutting linear endoscopic stapler. The pulmonary arteries, veins, and bronchus of each lung lobe were sealed and resected at once, and any air leakage or bleeding was not observed after the surgery. Compared to the conventional ligation method, the self-cutting linear endoscopic stapler has the advantage of significantly reducing the operation time and enabling simple and reliable sealing.

The present study is designed to investigate the antibacterial effect of the hot-water and various ethanol extracts from the leaves of Dendropanax morbifera L. (DML) against Porphyromonas gingivalis (P. gingivalsis). Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 30, 50 and 70% ethanol extract of DML against P. gingivalis decreased in a concentration-dependent manner. However, MIC and MBC of hot-water and 30% ethanol extract against P. gingivalis were the same as 3.13 and 6.25 mg/mL, respectively. In the bacterial growth inhibition test, the growth of P. gingivalis in the group treated with MBC and 2×MBC of DML hot-water extract was statistically significantly decreased from 6 h after incubation compared to the control group (p<0.001). From the results portrayed above, aqueous extract from DML at the concentration of 6.25 mg/mL can be usefully used to suppress infection of P. gingivalis, a major causative agent of periodontal disease.

A solid-phase competition enzyme-linked immunosorbent assay (ELISA), recombinant VP2 (rVP2) protein, and monoclonal antibody (mAb) were developed for the specific and sensitive detection of porcine parvovirus (PPV) antibodies in pig sera. A total of 1,544 sera samples were collected from breeding pig farms located in the Gyeongsangbuk-do Province in the Republic of Korea. The optimal operating conditions of SC-ELISA were as follows. The concentration of rVP2 proteins coated on the wells was 4 μg/mL, the swine sera were diluted 1:2, and the HRP-conjugated PPV VP2 mAb (9A8 clone) was used at 500 ng/mL. These results suggest that the SC-rVP-ELISA assay may be a valuable alternative to the current diagnostic tools used to detect PPV-specific monoclonal antibodies and broadly monitor PPV infections in domestic pigs at different breeding stages.

Admirandus multicavus Belogurov and Belogurova, 1979, a new record of free-living marine nematode species belonging to family Oncholaimidae is reported based on the specimens collected from the intertidal sediments of Korea. Admirandus multicavus is characterized by the following unique characteristics: cephalic setae (1-2 μm long), spicules (71-126 μm long) and gubernaculum (15-31 μm long), midventral preanal supplementary organ, three glandular duct openings of the Demanian system, and stoma length measuring twice the width. A comparative analysis of the biogeographical and ecological characteristics of Admirandus species is presented. DIC (Differential interference contrast) photomicrographs of the species are also provided herein. This report represents the first taxonomic analysis of the genus Admirandus Belogurov and Belogurova, 1979 discovered from the Korean seawaters.

To investigate the perceptions and attitudes of dental hygienists toward radiation safety management in Korea. A total of 800 dental hygienists were randomly selected for an anonymous survey, and 203 of them participated. The questionnaire items included the following: sex, career period, type of installed radiographic equipment, recognition of the diagnostic reference level (DRL), participation in radiation safety education, and attitudes toward radiation protection for both patients and dental hygienists. The participants were divided into two groups according to their years of experience (< 10 years versus ≥ 10 years). The difference between the groups was investigated according to frequency distribution. Fisher’s exact test or Pearson’s chi-square (χ2) test was used as appropriate. A regression analysis was performed to investigate the impact of wearing a thyroid collar for personnel protection during patient radiation exposure. The types of installed radiographic equipment included panoramic radiography (96.1%), cephalometric radiography (76.9%), intraoral radiography (72.9%), and cone-beam computed tomography (69.5%). Significant differences were observed in the learning pathway for the DRL (Fisher’s exact test, p < 0.05), satisfaction with radiation safety education (Pearson’s χ2 test = 5.3975, Pr = 0.02), and use of personnel radiation monitoring systems (Pearson’s χ2 test = 18.1233, Pr = 0.000) between the groups. Significant differences were also observed in personnel protection using a thyroid collar and patient protection during panoramic radiography (odds ratio = 14.2). Dental hygienists with more than 10 years of experience were more satisfied with radiation safety education and more interested in radiation monitoring. Considering career experience, customized, continuous, and effective radiation safety management education should be provided.

본 연구는 존 던의 사랑시를 통해 큐피디타스와 카리타스 사이의 전통적인 이원론이 재고되는 방식을 고찰한다. 던의 시가 서로 다른 종류의 사랑을 다루는 두 가지 범주로 대별될 수 있는 것은 사실이지만, 세속적 사랑과 종교적 사랑에 대한 그의 접근 방식은 서로 중첩되는 경향을 보인다. 던이 인생 경로에서의 변화 과정이나 회개 등의 개인사적 경험과 관련하여 성 어거스틴과 유사하다는 평을 받음에도 불구하고, 던의 초기/세속적 시와 후기/종교적 시 사이의 대조는 상대적이며 문제적인 측면을 보여 준다. 던의 시에 나타난 이런 경향은 이질적인 생각들을 독창적인 방식으로 결합시키고 통합하는 그의 형이상학적 상상력과 깊은 관계를 지니고 있다. 본 연구는 던의 사랑시를 변증법적 논리의 관점에서 이해하고자 시도하면서, 신체와 영혼 사이의 분리를 전제하는 어거스틴 의 ‘부정의 신학’과 던의 형이상학적 시학이 빚는 차이에 대해 논의한다. 던의 변증법적인 사랑의 철학은 큐피디타스와 카리타스의 융합이 이루어지는 과정을 보여 주며, 감각적이고 육체적인 경험을 통해 영적인 쾌락을 맛볼 수 있다는 논리를 궁극적으로 전달해 준다.