In this study, the longevity index was suggested for researches about social and environmental effects to human immorality and criteria for defining longevity area were developed using statistical analysis. Candidates indexes based on researches about the tend of human death ratio were investigated. As a result statistical analysis, ratio of population over 85 yearn old and over 65 years was selected as a longevity index. Statistical analysis on the longevity distribution at each district showed that 'eup' and 'myun' are appropriate spatial unit to study social and geographical characteristics of longevity. The spatial analysis using the ArcViewTM with the suggested index shows the time dependent variances of degree of immorality and spatial relationship between degree of immorality and human migration.

The purpose of this research was to develop evaluation index of regional development for producing potential values of rural regions besides agriculture on a commercial scale. Rural regions have potential values such as preserved nature and spatial advantages and so on. For regional development, potential values should be synthetically evaluated by objective and standard index for supporting decision. For evaluation of plural functions of rural regions, evaluation index of rural regions was developed based on rural amenity. Applicability of evaluation index was studied using GIS and natural break method.

To improve CO2 permselectivity, a modified silica membrane was prepared by chemical vapor deposition with tetraethoxysilane(TEOS)-ethanol-water, and TEOS-ethanol-water-HCI solution at 300-600℃. The silica was effectively deposited in the mesopores of a γ-alumina film coated on a porous α-alumina tube by evacuating the reactants through the porous wall. In this membrane, CO2 interacts, to some extent, with the pore wall, and CO2/N2 selectivity then exceeds the value of the Knudsen diffusion mechanism, while the membrane derived from TEOS alone has no CO2 selectivity. The silica membrane prepared from TEOS-ethanol-water-HC1 solution showed that CO2 permeance was 2.5×10-7㏖/s-1. m-2. Pa-1 at 30℃ and CO2/N2 selectivity was approximately 3. The CO2 permeance and selectivity was improved by enlarging the surface diffusion with modification of chemical affinity of the silica pores.

Purification of the isocitrate lyase extracted from Microbacterium laevaniformans was investigated. The isocitrate lyase was purified 43.6 folds by the following continuous treatment with ammonium sulfate fraction, DEAE-cellulose, DEAE-sephacel and Sephadex G-200 chromatography. The purified isocitrate lyase was showed to be a single protein band by polyacrylamide gel electrophoresis. The molecular weight of the purified isocitrate lyase was estimated 54,000 Da by the SDS-polyacrylamide gel electrophoresis. The Km and Vmax values for isocitrate were estimated to be 0.83mM and 0.33units/㎖, respectively. Activity of isocitrate lyase was inhibited by cystein-HCl and glutathione.

This study was conducted to investigate the treatment of wastewater from acetaldehyde manufacturing plant by activated sludge process with Micrococcus roseus AW-6, Micrococcus luteus AW-22, Microbacterium lacticum AW-38 and Microbacterium laevaniformans AW-41. The COD_Mn and BOD_5 of the wastewater were 5,260㎎/L and 6,452㎎/L, respectively. pH was 1.85. The main organic component in the wastewater was acetic acid which was contained 67,600㎎/L. Optimum dilution time for activated sludge process was shown 10 times. The specific substrate removal rate(K_e) was 1.95day^-1 and the nonbiodegradable matters(S_n) were 23.2㎎/L. Saturation constant (K_e) and maximum specific growth rate(q_max) were 1,640㎎/L and 2.33day^-1, respectively. Sludge yield coefficient(Y) and endogenous respiration coefficient(K_d) were 0.28㎎ MLVSS/㎎COD and 0.02day^-1, respectively. COD_cr removal efficiency was 91% for 1.95day of hydraulic retention time.

The increase of population and industrial activities had brought into eutrophication in the Nakdong river. A remarkable acceleration of eutrophication brought about serious problems for water supply. Therefore, for the purpose of conservation of water quality in the Nakdong river it is necessary to control nutrients. MBOD method was used to evaluate algal growth limiting factor and algal growth potential in the Nakdong river from June to August 1994. The modified biochemical oxygen demand(MBOD) depends on the amount of available inorganic nutrient and organic substrate during 5 day incubation in the dark at 20℃. The MBOD assay depends on inorganic nutrients such as P and N as well as reduced carbon and called the MBOD, the MBOD-P, and the MBOD-N, respectively. The results of bioassay by MBOD(Modified BOD) method showed that the MBOD, MBOD-P and MBOD-N value were found to be in the ranges of 3.8∼96.0 ㎎O_2/ℓ, 5.6∼94.0 ㎎O_2/ℓand 42.0∼220 ㎎O_2/ℓ, respectively. And the the bioassay value was found to be the highest in Koryong area and the lowest in Waekwan area throughout the Nakdong river. The variations of MBOD-P and MBOD-N value showed similar tendencies to the variations of phosphorus and nitrogen value, respectively. By MBOD method, the relationships of MBOD, MBOD-P and MBOD-N value were MBOD ≒ MBOD-P ≪ MBOD-N. The MBOD value was nearly equal to the MBOD-P value, and the MBOD-N value was 3 to 20 times more than the MBOD-P value, approximately. Therefore, in the Nakdong river, phosphorus was the limiting factor for algal growth during summer season. The algal growth potential as the concentration of chlorophyll-a in the summer was maximum 5 times more than standing crop as it.

A microcomputer system consisting of 16-bit microcomputer, PCL-711S interface board, censors, and converters have been set up in order to automatically measure temperature, humidity and weight loss which are major variables of storage of apple. This system was operated by PC-LabDAS software. It has been possible to measure continuously the weight loss of Fuji apple stored in CA with the weight converter made by a miniature load cell and a strain amplifier. The temperature was checked by a k-type thermocouple and Pt 100 RTD, and humidity by PQ653JAl humidity sensor. It has been possible to set up a linear equation which showers high correlationship between the estimate of temperature, weight humidity and the output of the converter in that r2 is more than 0.99. Transpiration rate, a significant factor of quality deterioration for CA storage of apple, can be estimated with these values.