본 연구는 2000년 12월에서 2007년 5월 사이 태안조간대에서 참굴(Crassostrea gigas)과 바지락 (Ruditapes phillippinarum)의 먹이원을 평가하기 위해서 굴과 바지락의 소화기관 내용물 속의 식물플랑크톤의 종 조성과 더블어 수증과 퇴적물의 식물플랑크톤의 군집조성을 격월로 조사했다. 조사기간 동안 수온은 7℃에서 23℃로, 염분은 34PSU에서 35PSU로, 각각 관찰되었다. 수중의

축소시스템 기법은 전체 구조의 거동을 나타내는 저차 고유모드를 근사화한다. 지난 연구에서 축소시스템을 구축하기 위한 2단계 축소기법을 제안하였다. 또, 기존의 2단계 축소기법을 반복적 IRS기법을 통해 중간 주파수 대역의 고유모드에 대한 해의 정확도를 높이는 방안에 대해 연구가 제안되었다. 본 연구에서는 기존의 향상된 2단계 축소기법에 다단계 부구조화 기법을 적용하는 기법을 제안한다. 첫 단계에서는 전체 시스템을 그래프 분할을 통해 계층적으로 부구조로 분할되고, 두 번째 단계에서는 각각의 부구조를 개선된 2단계 축소기법을 이용하여 축소한다. 각각의 축소된 분절화된 고유치문제의 조합을 총해 최종적 축소시스템을 구축하고 이렇게 구한 축소된 고유치 문제를 란초스 기법(ARPACK)을 통해 해석한다. 최종적으로 제안된 기법의 성능을 수치 예제를 통해 검증한다.

To screen highly active Bacillus thuringiensis isolates against Spodoptera litura (Lepidoptera, Noctuidae), 46 B. thuringiensis was isolated from 115 samples obtained from several crop soils. Especially, B. thuringiensis subsp. kurstaki CAB133 and CAB162 isolates showed 100% mortality against S. litura. LD50 values of CAB133, CAB162 and HD-1 strains of B. thuringiensis subsp. kurstaki were 0.089, 3.144 and 0.513 ㎍/㎖ against 2nd larva of μ S. litura, respectively. The weight of 3rd larva of S. litura which were fed crystal inclusion protein (1.267 ㎍/㎖) with B. thuringiensis subsp. kurstaki CAB133 was about 30 times lass than control group. CAB133 and CAB 162 strains of B. thuringiensis subsp. kurstaki which were taken a highly toxity against S. litura were analyzed by SDS-PAGE, and estimated the molecular weight of the Cry proteins. Their serological identification by H serotypes were showed B. thuringiensis subsp. kurstaki (3abc) type.

Bacillus thuringiensis with selected high toxicities against tobacco cutworm, Spodoptera litura were isolated from domestic soils. When being observed under a phase-contrast microscope, the insecticidal crystal proteins were showed a bipyramidal crystal types. New CAB 109 isolate was identified to B. thuringiensis subsp. aizawai in the H serotype. As a results of insecticidal activities between CAB 109 isolate and 3 existing ready-made products against 3rd larva of S. litura, CAB 109 isolate showed 100% mortality with spore concentration (1.3×10⁷ cfu/㎖). It was a very high insecticidal activity compared with a existing ready-made B. t. products. LD₅₀ values of CAB 109 isolate was 9.78×10⁵, 6.87×10⁶ and 1.83×10⁷ cfu/㎖ spore concentration against 2nd, 3rd and 4th larva of S. litura, respectively. Unlike Plutella xyfostella, S. litura was slowly died after application up to 7 days. The weight of S. litura larva applied with CAB 109 isolate were 6-7 times less than controlled group. Even though it didn"t die, it did not grow into next larva. The result observed with scanning electron microscope was that CAB 109 isolate of B. t. aizawai formed a typical bipyramidal crystal protein type. Otherwise, when CAB 109 isolate was examined with SDS-PAGE and with trypsin, there was no difference between CAB 109 strain and ready-made products of B. thuringiensis.

For the development of friendly environmental control of the beet armyworm, Spodoptera exigua that is too hard to control in the field, 25 insecticides were chosen from 58 registered to the beet armyworm, and bioassayed. There are 12 insecticides with neurotoxical activities, 10 with insect growth regulators and 3 Bacillus thuringiensis products. Among 12 insecticides with neurotoxical activities, mortality of S. exigua was 100% with emamectin benzoate (EC) and indoxacarb (WP) within 3 and 5 days after application, respectively. Otherwise, WG and SC of indoxacarb, Indoxacarb + etofenprox (WP) and pyridalyl (EW) were showed up to 91%. Methoxyfenozide + spinosad (SC) was better than any other insect growth regulator as 100% mortality within 3 days after application. And methoxyfenozide (WP), tebufenozide (WP) and methoxyfenozide (SC) were 92% by 5 days. However, 3 kinds of B. thuringiensis products were showed under 35% mortality within 5 days from first spray.