Biodiesel is a remarkable alternative fuel for the diesel engine among various countries. Various studies are carried out on the environmental and economic effects as well as the nature of diesel engine fuel biodiesel in the combustion process and emission characteristics. In this study, CRDI diesel engines used diesel fuel and 20 % biodiesel blend fuel (BD 20 %) as fuel, with City-mode exceeding 300 hours. Engine performance and emissions were sampled at one-hour intervals for analysis. The engine was inspected after testing to check the engine parts (valves and injectors). It was concluded that 300 hours of operation using BD 20 % does not cause abnormal deterioration of the engine or abnormal changes in engine power and exhaust gas.

The purposes of improving air pollution and suggesting energy resources have intensified research on various alternative energy for diesel combustion engines to solve severe environmental influences caused by air pollution. In this study, the applicable possibility of biodiesel and oxygenates (EGBE) was investigated as a cuspy method on decreasing the smoke emission. The smoke emission of blending fuel (biodiesel and EGBE) was reduced in comparison with commercial diesel fuel and it was reduced almost 63% at 2000 rpm, full load. But torque and brake specific energy fuel consumption rate didn't have any large changes. Also, the effectiveness of exhaust gas recirculation (EGR) for the reduction of NOx emission has been investigated. It was shown that simultaneous reduction of NOx and smoke emission was achieved with biodiesel(90%), oxygenates (10%) and cooled EGR rates (10%) in an IDI diesel engine.

By analyzing the status of Korean tire labeling regulation and comparing the performance indicators of tire labeling in different countries, this paper expounds the serious impact of tire noise on traffic noise pollution, and puts forward that in order to improve the comfort of car ride, control traffic noise pollution and build a green tire industry, the tire labeling system should be improved, and the corresponding requirements for tire rolling noise should be put forward according to the road conditions in Korea.

The study on the tire noise due to the On-Board Sound Intensity test method. The object of this project is to prepare the countermeasure of the noise reduction according to collect tire/road noise data under various influencing factors by using On-Board Sound Intensity test method. To accomplish the object ; A automotive is used as a test platform to test tire/road noise by changing different types of tires and using On-Board Sound Intensity test system. The influence of inflation pressure, road, load, speed and pattern type on tire/road noise is compared and analyzed. At the same time, the mechanism of tire/road noise is analyzed, which provides a experimental basis for tire noise reduction.

To preserve the superior genetic resources and restore the endangered species, Somatic cell nuclear transfer (SCNT) has been used widely. In Korea, the research of dog cloning has made outstanding achievements including the production of the world`s first cloned dog. Sapsaree (Sapsalgae), the representative dog of Gyeongsan-si was designated as a Korea natural monument (No. 368). This male dog used in this study has azoospermia due to unknown cause. In this study, the aim was to confirm the cause of infertility in the cell donor dog and to evaluate the reproduction potential of dog cloning using infertile male dog by SCNT. First, to confirm the infertility of the cell donor dog, the reproductive history and the testis were evaluated. The breeding histology was not recorded in individual document. In histopathology, the Sertoli cell tumor was confirmed in biopsy of the cell donor dog after death. But, these tumors are predominantly in older dogs. Second, we produced the cloned dogs with the somatic cells of the infertile dog and the appearance was similar with the cell donor dog. Also, microsatellite analysis confirmed the genetic relationship between the cell donor and clone dogs. Third, the potential breeding capacity of the cloned dog was confirmed. In T4 assay, the normal dog (same age with cloned dogs), cell donor dog, and cloned dogs was investigated. The cell donor dog with azoospermia had very low T4 level, and cloned dogs showed higher level of T4 than normal dogs. In CASA, There was no significant difference in sperm motor ability between normal dogs and cloned dogs. As a result, cloned dogs produced by SCNT had no problem regarding the reproductive function of the testis. In AI experiment, the semen of clone dogs was used to fertilize a natural female bitch and was diagnosed pregnancy by ultrasonography. In total, 7 puppies were born by normal delivery (male: 3, female: 4). In conclusion, this study confirmed that the reproduction problem of non-genetic infertility can generate a normal descendant by SCNT. Also, the first successful research to restore infertile dogs was completed. Furthermore, SCNT would be useful for the restoration of endangered species and application of superior traits.