Microorganism such as a bacterium, virus, fungus, or microscopic nematode worms can be used in agricultural practices to control of elimate pests that can inflict damage to a plant. Agents used as biopesticides include parasites, predators, fungi, bacteria and viruses. Each agent has a specific mode of operation used to control pests. They are being recommended and used as components of IPM programs in the production of high-value specialty crops such as fruit, nut, vegetable, vine, ornamental, and turf crops. The global pesticide market was valued in 2013 at approximately $54.2 billion. Of that total, nearly 30% is comprised of herbicides to control weed populations. This segment of the market is projected to expand through 2014 for both synthetic pesticides and biopesticides. The projected market percentage should reach nearly 6% by 2014. Biological control agents are termed as ‘Biological Pesticides’ by the Korean government. At present there are 33 registered products in Korea under this category, among these 19 are fungicidal, 13 are insecticidal and only 1 have herbicidal activity. The total number of domestically produced biological pesticides is 19 including Bts whereas rest of them are imported. Mostly these are originated from microorganisms. Organic agriculture materials are 1,300 registered products in Korea. Approximately 500 companies have registered your product is a very small company and the parent company, except for the more than 20 companies.

With the increasing trend of global trades and protection of agro-ecosystem in importing and exporting countries, quarantine and pre-shipment(QPS) fumigation for perishable commodities are critical in terms of quality maintenance of fumigated commodities as well as eradicating quarantine pest. There are limited use of methyl bromide(MB) fumigation on fruits and vegetables due to not only worker safety issue at low temperature condition but phytotoxic damage to valuable commodities. We are here presented that commercial development of methyl bromide (MB) alternatives such as ethyl formate and phosphine gas formulated with carbon dioxide, that are increasing consumer and worker safety and avoiding phytotoxic damage in routine MB fumigation.

The most industry players were still building a case for biopesticides as an important sector of the crop protection industry, most bigger players were making guarded statements about their intentions in the sector, and the headline proclaimed that biopesticides were no longer a fringe sector. Within months, the larger companies started getting seriously involved through acquisitions and licensing deals. The second special issue that came out in 2013 gauged the momentum that the sector quickly achieved and it was becoming clear that biopesticides would figure in the plans of most of the big crop protection companies. The momentum that started in 2012 was sustained throughout 2013 and the first four months of 2014 in the forms of deals as well as acquisitions. The Europe-based majors consolidated the acquisitions made during the previous year and went about submitting registration dossiers for the acquired products and formulating business plans incorporating biopesticides into their overall strategy. In 2013, big US-based companies became active. Monsanto acquired US agricultural biotechnology company, Agradis, which has a collection of thousands of plant-associated microorganisms; formed a partnership with Danish company Novozymes to develop and commercialise biopesticides worldwide; and supported the establishment of a US company, Preceres, aimed at the development of delivery methods for RNAi-based biopesticides. The global companies believe that biologicals will provide new growth opportunities in agriculture and are looking at market opportunities in all crops and the needs of its crop protection business.

For replacement of methyl bromide(MB) in Korea, there are several chemical options being introduced and developed in preplant and postharvest sections. In soil case, methyl-isothiocyanate(MITC) generating fumigant such as dazomet and metham sodium are currently being adapted for prevent from spreading soilborne disease and nematode, although there will be more successful application technology needed to develop in domestic circumstances. Also, ethanedinitrile(EDN), is newly developed chemical, could be one of the options to eradicate notorious soilborne diseases and nematodes near future. Ethyl formate(EF) now is showing the potential to replace MB in terms of consumer and worker safety and unwanted phytotoxic damage in routine MB fumigation. A phosphine gas(PH3) could be the last option to protect the import and export stored grains from stored grain insects and to solve the current issue such as PH3-resistant insect and consumer safety issues to replace metal phosphide. EDN also should be extended its use in not only timber and log fumigation in quarantine purpose but in use of national heritage and forest to keep wood infested pest safer. This presentation will be good opportunities to share the information and discuss with further research needs in current MB alterative program.

The aim of the present study was to evaluate the use of an immunochromatographic test (ICT) strip using recombinant MPB70 (rMPB70) protein as a complementary tool for the diagnosis of naturally occurring tuberculosis in cattle. The study was performed on 249 cattle from populations known to be free from Mycobacterium bovis (M. bovis) and 119 cattle with M. bovis infection, confirmed postmortem. Compared to reference standards (culture isolation and/or visible lesion), the sensitivity of ICT was 94.12% (95% CI: 89.89∼98.35%) while the specificity was 96.80% (95% CI; 94.62∼96.82%). The findings indicate that the ICT strip is efficient for diagnosing bovine tuberculosis in cattle from Korea.

Biopesticides are pesticdide derived from natural materials such as animals, plants, bacteria, and certain minerals. Microorganism such as a bacterium, virus, fungus, or microscopic nematode worms can be used in agricultural practices to control of elimate pests that can inflict damage to a plant. Agents used as biopesticides include parasites, predators, fungi, bacteria and viruses. They are being recommended and used as components of IPM programs in the production of high-value specialty crops such as fruit, nut, vegetable, vine, ornamental, and turf crops. The global pesticide market is growing at a 15.8% for the period of 2012 to 2017. Synthetic insecticide use is projected to continue to decline, particularly with the increased use of GM seeds. GM crops are competiong with biopesticides due to disease and pest resistance and complementing for production of chemical residue free crops. Biopesticedes represent a strong growth area in the global pesticide market. Low registration cost and time for development of new active ingredient are major growth drives for the biopesticides market. Bacterial biopesticides control over 70% of microbial biopesticdes market share. Bacillus thuringiensis (Bt) is the most popular bioinsecticide worldwide. The global market for botanical biopesticide is estimated to grow at a CAGR of 14% and semio-chemicals is expected to grow at a CAGR of 16.1% from 2012 to 2017. The global microbial biopesticides market was valued at $862 million in 2011 and is expected to reach $2,606 million by 2017. Global biopesticides market is dominated by bioinsecticides with around 46% share in 2011.

For replacement of methyl bromide(MB) in Korea, VapormateTM, a gas formulation of ethyl formate(EF) with carbon dioxide, are firslty introduced in import bananas and VivakilTM, a gas formulation of phosphine(PH3) with carbon dioxide, are ready to commercial use in export cut flowers. Their applications are being extended for other import and export commodities. Ethyl formate have been shown to have effectiveness short exposure time(<4hr) but it has a limited application at low temperature for TSM(Two spotted spider mites) due to some phytotoxic to strawberry. Phosphine gas has demonstrated their efficacy to TSM at low temperature but only meets quarantine requirement when applying longer fumigation time(>24hr) to control CA(cotton aphids). We firstly evaluated two MB alternatives, EF and PH3, have enhansive effect within short exposure time(4hr) at low temperature(5℃) for controlling both TSM and CA without any injury to export strawberries.

Opioid receptors have been pharmacologically classified as µ, δ, κ and ε. We have recently reported that the antinociceptive effect of morphine (a µ-opioid receptor agonist), but not that of β-endorphin (a novel µ/ε-opioid receptor agonist), is attenuated by whole body irradiation (WBI). It is unclear at present whether WBI has differential effects on the antinociceptive effects of µ-, δ-, κ- and ε-opioid receptor agonists. In our current experiments, male ICR mice were exposed to WBI (5Gy) from a 60 Co gamma-source and the antinociceptive effects of opioid receptor agonists were assessed two hours later using the hot water (52℃) tail-immersion test. Morphine and D-Ala2,N-Me-Phe4,Gly-ol-enkephalin (DAMGO), [D-Pen2-D-Pen5]enkephalin (DPDPE), trans-3,4-Dichloro-N-methyl-N-[2-(1-pyrrolidinyl)- cyclohexyl]¬benzeneacetamide (U50,488H), and β-endorphin were tested as agonists for µ, δ, κ, and ε-opioid receptors, respectively. WBI significantly attenuated the antinociceptive effects of morphine and DAMGO, but increased those of β-endorphin. The antinociceptive effects of DPDPE and U50,488H were not affected by WBI. In addition, to more preciously understand the differential effects of WBI on µ- and ε¬opioid receptor agonists, we assessed pretreatment effects of β-funaltrexamine (β-FNA, a µ-opioid receptor antagonist) or β-endorphin1-27 (β-EP1-27, an ε-opioid receptor antagonist), and found that pretreatment with β-FNA significantly attenuated the antinociceptive effects of morphine and β endorphin by WBI. significantly reversed the β-EP1-27 attenuation of morphine by WBI and significantly attenuated the increased effects of β-endorphin by WBI. The results demonstrate differential sensitivities of opioid receptors to WBI, especially for µ- and ε-opioid receptors.

염산매질에서 산화/환원제를 사용하여 Pu 산화수를 조절한 후, UV-Visible-Near IR 분광기를 이용하여 Pu(III, IV, V, VI) 산화수에 대한 흡수스펙트럼을 측정하여 그 분광학적 특성을 고찰하였다. Pu(III)으로 조절하기 위하여 환원제인 HCl를 사용하였으며, Pu(IV)와 Pu(VI)로 조절하기 위하여 산화제인 및 를 각각 사용하였다. 또한 Pu(VI)로 조절된 용액에 환원제인 HCl를 사용하여 Pu(V)로 조절하였다. Pu(III)와 Pu(IV)의 대표적인 흡수피크는 470 nm 및 600 nm에서 각각 관찰되었고, Pu(VI)와 Pu(V)의 특성피크는 830 nm 및 1135nm에서 각각 관찰되었다. Pu(III, IV, VI) 산화상태의 시간 경과에 따른 흡수스펙트럼 변화는 관찰되지 않았으나 Pu(V)의 경우 매우 불안정하여 생성되자 마자 Pu(III)로 변화되었다.

To reveal the relationship between the changes in the growth and photo- synthesis induced by low dose radiation, red pepper (Capsicum annuum L.) plants were serially irradiated three times with gamma rays of 0.5, 1, 2, 3, and 4 Gy. The plant growth was mo

Purpose: This study sought to explore current status of children’s swimming pool in Korea and to generate insights regarding safety management of children’s swimming pool. Research design, data, and methodology: The study reviewed literatures including documents of Ministry of Culture, Sports and Tourism and sport facility-related articles. Furthermore, the studycollected additional statistics via search engines. Results: First, numbers of swimming pools have been dramatically increased since 2016 and Korea experienced an increase of 37% from 2010 to 2019. Of the 611 indoor swimming pools, most swimming pools are located in Gyeonggi province with 27.7% (n=169). Seoul and Gyeonggi-do have the highest proportion of swimming pools in Korea. Second, in types of swimming pools, 47.6%(n=291) was children’s swimming pool which is the highest proportion in the nation. Children’s swimming pools are also mostly located in Seoul and Gyeonggi province with 57%. Third, of 372 profit swimming pools, children’s pools show the highest number with 78%(n=291). Conclusions: Children’s swimming pools are picking up faster than the other types of swimming pool. Yet, safety-related studies are still lack. This provides motivations to produce managerial insights from academia and business sectors. Moreover, more studies of legal issues should be conducted. Implications were discussed.

본 연구에서는 보건계열 대학생의 열등감을 관리하는 집단상담 프로그램을 활용하여 열등감에 대한 대학생의 긍정적‧부정적 반응에 미치는 효과를 확인하고자 하였다. 2018년 11월 20일에서 2019년 1월 2일까지 S 대학교 보건계열 대학생 총 30명을 대상으로 실험집단과 통제집단으로 나누어, 열등감관리 집단상담 프로그램을 주 2회, 120분씩 총 8회기 실시하였다. 프로그램의 효과성을 검증하기 위하여 열등감반응 척도를 이용하여 사전, 사후, 추후로 실시하였다. 분석 결과, 본 연구는 열등감관리 집단상담 프로그램이 대학생들의 열등감에 대한 부정적 반응을 유의하게 감소시켰으며, 열등감에 대한 긍정적 반응을 유의하게 증가 시킨 것으로 나타났다. 이러한 결과는 열등감관리 상담 프로그램을 통해 또래와의 비교에서 느끼는 대학생들의 열등감에 대한 부정적인 태도를 낮추고 긍정적인 태도를 높일 수 있음을 시사한다.

Floral scents and metabolites from cut flowers of 14 peony cultivars (Paeonia lactiflora Pall.) were analyzed to discriminate different cultivars and to compare the Korean cultivar with the other cut peonies imported to Korea using electronic nose (E-nose) and Fourier transform infrared (FT-IR) spectroscopy combined with multivariate analysis, respectively. Principal component analysis (PCA) and discriminant function analysis (DFA) dendrogram of peony floral scents were not precisely same but there were 3 groups including same cultivars. PCA and partial least squares-discriminant analysis (PLS-DA) dendrograms of peony metabolites showed that different cut peony cultivars were clustered into two major groups including same cultivars. Fragrance pattern of Korean ‘Taebaek’ was classified to same group with ‘Jubilee’ on the PCA and DFA results and its metabolite pattern was clearly discriminated by the PCA and PLS-DA compared to the other cultivars. These results show that the 14 peony cut flowers could be discriminated corresponding to their chemical relationship and the metabolic profile of Korean ‘Taebaek’ has distinctive characteristics. Furthermore, we suggest that these results could be used as the preliminary data for breeding new cut peony cultivars and for improving the availability of Korean cut peony in cosmetic industry.

Due to strong binding, optical clarity, adhesion to many surfaces, toughness and flexibility polyvinyl butyral(PVB) resin films are commonly used in the automotive and architectural application as a protective interlayer in the laminated glass. Worldwide million tons of PVB waste generated from end-of-life automotive associated with various environmental issues. Stringent environmental directive, higher land costs eliminate landfilling option, need a sustainable, environment-friendly technology to recycle these solid wastes. In our current study, we have developed a mechano-chemical separation process to separate PVB resins from glass and have characterized the separated PVB through various techniques, i.e., scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy (EDS), infrared spectroscopy (IR) and nuclear magnetic resonance spectroscopy (NMR). Feasibility for reuse of these recycled PVB has been investigated. The technology developed in our laboratory is sustainable, environment-friendly, techno-economical feasible process, and capable of mass production (recycling).

Native of soybean aphid (Aphis glycines Matsumura) is an Asia and aphid is one of the dangerous pests in soybean [Glycine max (L.) Merr.]. High density aphid populations can reduce crop production by causing severe damage. The objective of this study was evaluation of resistance to the soybean aphid in soybean cultivars and germplasms. A total of fifty five soybean cultivars or germplasms, including two susceptible and two resistant check varieties, were infested to evaluate their resistance in the field cage and greenhouse test by aphid colonies which derived from wild collected one soybean aphid biotype in Korea. The average number of reproduced soybean aphid was evaluated with 62.7 aphids in the resistant check variety PI 567598B and also estimated with 1,807 aphids for susceptible check variety Williams 82. In soybean varieties and germplasms, the average reproduced soybean aphid populations ranged from the lowest 497 aphids for Junjeori to the highest 3,862 aphids for Mansu. About seventy six percent of soybean cultivars and germplasms were shown high density soybean aphid populations when compared with another susceptible check variety PI 567543C in the field cage test. From the greenhouse test to evaluate aphid index, 87.3% of soybean cultivars or germplasms presented aphid index with 9.0. No soybean cultivars and germplasms were observed with soybean resistant phenotype when regarded a aphid resistant level as less than 10% aphid reproductions compared with susceptible check Williams 82. Although no Korean soybean cultivars were identified with resistant trait to the soybean aphid, we found one great resistant genetic resource PI 567598B in this study. This result will be helpful to further study for providing useful genetic information for soybean researchers.