경상북도에 위치한 산림유전자원보호구역내 2개 지역(영주지역의 신갈나무, 울진지역의 소나무)과 경기 포 천시에 위치한 생물권보전지역내 2개 지역(포천지역의 신갈나무와 소나무)에서 2016년부터 2017년까지 함정 트랩(Pitfall trap)을 매년 5회씩 5반복하여 각각 비교하였다. 그 결과 산림유전자원보호구역에서는 27과 106속 160종 2,665개체가 확인되었으며, 종풍부도는 20.28, 종균등도는 0.266, 종다양도는 1.355, 우점도는 0.265로 우점 종은 납작먼지벌레속 sp.2 (Synuchus sp.2)이였다. 또한, 생물권보존지역에서는 16과 49속 77종 4,834개체가 확 인되었으며, 종풍부도는 8.958, 종균등도는 0.301, 종다양도는 1.31, 우점도는 0.669로 우점종은 윤납작먼지벌레 (Synuchus nitidus)였다. 산림의 중요 지역 간에 지표성 딱정벌레의 군집을 비교함으로써 환경변화에 따른 산림 보존 프로그램을 수행하는데 유용한 기초 자료로 활용될 것이다.

The occurrence level of Metcalfa pruinosa, Halyomorpha halys, and Spodoptera litura was investigated in persimmon tree and bean cultivation fields in 14 cities and counties of the Jeonbuk region in June and July. Metcalfa pruinose was visually inspected, while Halyomorpha halys and Spodoptera litura were investigated by installing pheromone traps and counting the number of captured individuals once, seven days after trap placement. Metcalfa pruinose nymphs occurred in eight cities and counties, which showed an increase compared to the previous year, and Halyomorpha halys nymphs had higher occurrences in the plains, with an average count of 5.5. The average count of Spodoptera litura was 26.7, which was lower than the previous year.

Ricania sublimata are insects invaded in South Korea from China 2011. It has been happening continuously since invaded and It is established and continues to increase and occur in various crops such as persimmons, peaches, and apples. The nymphs and adults of R. sublimata causes damage by sucking the stems and leaves of trees. Yellow, blue, and white sticky traps (25x15cm) were installed in the 1000m2 Omija(Schizandra Chinesis Baillon) orchard september 2020 and the trap captures by color was investigated. In addition, yellow sticky traps were installed at heights of 1m, 1.7m, and 2m, and the trap captures according to the height was investigated. As a result of attraction according to the color of the sticky trap, yellow trap was the most attractive, blue and white traps had little attraction. And result of capture according to the yellow sticky trap height the most were caught at 1.7m height, followed by 1m and 2m in that order. Therefore, it is considered reasonable to investigate the density and monitoring of R. sublimata using yellow sticky trap.

벚나무류 수목은 생활권 수목(도시공원, 가로수 등)으로 전국에서 가장 많이 식재되고 있으며 전라 남도 또한 벚나무류 수목이 가로수 중 가장 많은 비율을 차지하고 있다. 복숭아유리나방은 농업환경 외에도 생활권 녹지공간에서도 벚나무류 수목을 가해하여 피해를 발생시키고 있는 것으로 보고되고 있으나, 생활권 수목과 산림에서의 관련 연구는 그 필요성에 비해 미흡한 상황이다. 본 연구는 전라남 도 내 생활권 수목을 기주로 하여 발생하는 복숭아유리나방의 발생소장을 조사하여 생활권 녹지공간 을 관리하는 기초자치단체들의 효용성 있는 방제 전략 수립에 기여하고자 한다. 복숭아유리나방의 발생 확인을 위해 육안 확인이 용이한 피해흔을 세 가지로 분류하였으며, 성페로몬 루어와 트랩으로 복숭아유리나방 성체를 유인･채집하였다. 그 결과, 4월 중순 첫 우화를 확인하였으며, 5월 중순과 8월 중순에 2번의 우화 최성기를 확인하여 이때의 유효적산온도를 조사하였다. 또한, 채집된 개체들 의 종 확인 및 유전적 다양성 확인을 위해 mitochondrial 내 cytochrome oxidase subunit I (COI) 유전자 염기 서열의 분석을 수행하였다.

Sweet pepper(paprika) belongs to the genus Capsicum, and is one of the most important export product from Korea to Japan and Southeast Asia. So it is important to eradicate plant quarantine pests before export sweet pepper. Aphids, whiteflies and mites are major pests that can damage to sweet peppers. Fumigation is normally used to eradicate pests in plant quarantine, but phytotoxicity may can be appeared that affect the quality of the product. Low-temperature treatment, one of the most popular physical treatment, can reduce crop damage to preserve product quality, but it takes long time to kill pests, which can cause quality degradation. In this study, phytotoxicity of fumigants, phosphine(PH3), ethyl formate(EF) and PH3+EF on sweet peppers was investigated to use as basic data for physicochemical treatment. When treated with more than 35 mg/L of EF, phytotoxicity was occurred, and was not occurred with PH3. When low-temperature of 1.7 degrees treated for 15 days after fumigation, it seems to be no direct damage from low-temperature treatment. But quality of top of sweet pepper was decreased from 7 days after fumigation.

The fall armyworm (FAW), Spodoptera frugiperda (Lepidoptera: Noctuidae), which is native to tropical and subtropical regions of the Western Hemisphere is now annually arrives in Korea. In this study, we developed loop-mediated isothermal amplification (LAMP) assay, one of the main merits of which is a rapid identification of target species. Five among 11 FAW-specific loci tested successfully provided a consistent reaction when ten FAWs, which were collected from eight localities in four countries were tested, whereas the 13 non-target species were not amplified. To increase in-field applicability of the method all life stages, reaction time, and different periods after death was tested using the quick extracted DNA. Our FAW diagnostic protocol can be completed within 30 min, from the process of extracting genomic DNA from an egg or a 1st instar larva to species determination.

국내 유입 가능성이 높은 검역 관리해충인 Spodoptera eridania 및 S. ornithogalli는 전 세계적으로 토마토, 콩 등 여러 종의 작물을 가해하는 광식성 해충이다. 이에 따라 국내 유입 시 해당 작물에 높은 경제적 피해를 입힐 가능성 이 있으므로 신속 정확한 진단이 필요한 실정이다. 따라서 본 연구에서는 상기 두 종을 대상으로 현장 활용이 가능한 LAMP 진단법 개발을 수행하였다. 표적종 두 종 및 비표적종 11종(국내 발생 Spodoptera 종 및 동일 기주 가해종 등)의 전장유전체 정보를 확보한 후 비교 분석을 통해 각 표적종 별 특이적 영역을 확보한 후 해당 영역을 대상으로 LAMP 프라이머를 제작하였다. DNA 농도 10 ng/μL, 반응시간 40분을 기준으로 LAMP 진단을 수행한 결과, Spodoptera eridania는 5개의 LAMP 진단 마커를 개발하였고, S. ornithogalli는 3개의 LAMP 진단 마커를 개발하였다.

Because of recent reports about phosphine resistance problem, development of effective fumigation method to control grain pests became very important. In this study, a chemical treatment, ethyl formate fumigant treatment, and a physical treatment, atmospheric control, were attempted as alternative solutions to this problem. In this study, for CA(Controlled atmosphere) treatment, 99.999% nitrogen was used to create a hypoxic condition with less than 5% oxygen, and for EF, the treatment concentration was 10 mg/L to 80 mg/L. As a result of the study, in CA single treatment, adult insects showed a mortality rate of less than 10% even after 2 weeks of treatment, and pupae and larvae showed a mortality rate 71% and 34%, but eggs showed a mortality rate of 100%. In EF single treatment, adults and larvae showed a 100% mortality rate at 80 mg/L, but eggs showed a 50% mortality rate and pupae were not affected. Considering the results, CA single treatment is not suitable for controlling Tribolium castaneum because of long treatment period, and in the case of EF single treatment, additional researches on longer treatment time is needed.

Ceriagrion nipponicum (Odonata: Coenagrionidae) is listed as climate-sensitive indicator species in Korea and now expands its range northward. In this study, we sequenced complete mitochondrial genome (mitogenome) of the species collected from South Korea for comparatively analysis in damselflies and to detect the genes suitable for subsequent population genetic study. Comparison of the mitogenomes from two geographic samples of C. nipponicum showed the highest variation in ND4 and ND1, whereas no variation was detected in COI, warranting usefulness of the two genes for subsequent population-level study. Phylogenetic analysis using 13 PCGs and 2 rRNAs in Zygoptera showed non-monophyletic Coenagrionidae, forming two groups.

Bombyx mandarina (Lepidoptera: Bombycidae), the presumed ancestor of B. mori, has long been a subject of study to illustrate the geographic relationships in connection with origin of B. mori. We report 97 mitochondrial genome (mitogenome) sequences of B. mandarina collected from Korea and Japan. Phylogenetic and population genetic analyses showed that all individuals of B. mandarina collected in Korean localities formed a strong group together with all individuals originated from northern China (mainly north of the Qinling-Huaihe line) and some of southern China. This group was placed as the sister group to B. mori strians suggesting that this group had been served as an immediate progenitor for B. mori.

In Korea, both Sympetrum depressiusculum Sélys, 1841 (Odonata: Libellulidae) and Sympetrum frequens Sélys, 1883 are recorded. However, the identity of Korean populations and the validity of listing the two species have not yet been settled. In this study, we collected 74 individuals from Kroea, Russia, the Netherlands, and Japan. These were sequenced for COI, 16S rRNA, and ITS region. Major morphological characters and phylogenetic, network, and structure analyses all consistently suggest that Korean populations form a single species. Consequently, it could be valid to treat Korean populations as one species, S. depressiusculum, by applying the senior name.

Ramulus mikado (Rehn, 1904) is an environmentally problematic species that has recently caused a severe outbreak on the mountainsides of the Seoul metropolitan area, resulting in significant damage to forests and causing inconvenience to people in the city centre vicinity. In this investigation, we analysed the genetic diversity through sampling in the outbreak region. The outcomes confirmed the efficiency of COI for species identification and the usefulness of haplotype analysis in examining regional variation.

Background: Outcome measures (OMs) are essential components of evidence- based practice as they help in implementing interventions and assessing changes in a patient's status before and after treatment. However, many therapists do not utilize OMs in their practice, and research on the factors that influence physical therapists' decisions regarding the use of OMs primarily consists of quantitative or survey-based studies. Objectives: The purpose of this study was to select research studies on OMs use among physical therapists through a systematic review, identify questionnaire characteristics, and assess the quality of items collection and research. Design: A systematic review. Methods: This study systematically collected survey studies on the use of OMs from five databases (KMBase, RISS, KCI, Pubmed, and Cochrane) based on the criteria of PRISMA's systematic review flowchart. The quality of the studies was assessed using the AXIS Tool. Results: A total of eight studies were selected. In most of the studies, the questionnaires were developed internally, validated through preliminary research, and reliability testing was not conducted. The items used within OMs were divided into 10 categories, with the highest frequency of questions centering around how to use OMs, and the lowest frequency regarding work environment and reasons. All eight studies were determined to be of good quality. Conclusion: The collected OMs questionnaires revealed variations in the item frequency. Future qualitative research and participant feedback are needed to develop a consistent questionnaire and validate its validity and reliability.

Silene fissipetala Turcz., which is native to Continental China and Taiwan, was newly found in Chungcheongnam-do, Korea. S. fissipetala is similar to the related taxa S. antirrhina L., S. armeria L., and S. koreana Kom. in that it has glutinous zones. However, S. fissipetala is distinguished from the congeneric species by the presence of laciniate at the petals. The species grows on slopes and roadsides, suggesting that it is likely to have been introduced through the installation of green sites and road construction. A precise description, photographs, voucher specimens and a key to related taxa are provided.

The Fourth Industrial Revolution and sensor technology have led to increased utilization of sensor data. In our modern society, data complexity is rising, and the extraction of valuable information has become crucial with the rapid changes in information technology (IT). Recurrent neural networks (RNN) and long short-term memory (LSTM) models have shown remarkable performance in natural language processing (NLP) and time series prediction. Consequently, there is a strong expectation that models excelling in NLP will also excel in time series prediction. However, current research on Transformer models for time series prediction remains limited. Traditional RNN and LSTM models have demonstrated superior performance compared to Transformers in big data analysis. Nevertheless, with continuous advancements in Transformer models, such as GPT-2 (Generative Pre-trained Transformer 2) and ProphetNet, they have gained attention in the field of time series prediction. This study aims to evaluate the classification performance and interval prediction of remaining useful life (RUL) using an advanced Transformer model. The performance of each model will be utilized to establish a health index (HI) for cutting blades, enabling real-time monitoring of machine health. The results are expected to provide valuable insights for machine monitoring, evaluation, and management, confirming the effectiveness of advanced Transformer models in time series analysis when applied in industrial settings.