Because of recent reports about phosphine resistance problem, development of effective fumigation method to control grain pests became very important. In this study, a chemical treatment, ethyl formate fumigant treatment, and a physical treatment, atmospheric control, were attempted as alternative solutions to this problem. In this study, for CA(Controlled atmosphere) treatment, 99.999% nitrogen was used to create a hypoxic condition with less than 5% oxygen, and for EF, the treatment concentration was 10 mg/L to 80 mg/L. As a result of the study, in CA single treatment, adult insects showed a mortality rate of less than 10% even after 2 weeks of treatment, and pupae and larvae showed a mortality rate 71% and 34%, but eggs showed a mortality rate of 100%. In EF single treatment, adults and larvae showed a 100% mortality rate at 80 mg/L, but eggs showed a 50% mortality rate and pupae were not affected. Considering the results, CA single treatment is not suitable for controlling Tribolium castaneum because of long treatment period, and in the case of EF single treatment, additional researches on longer treatment time is needed.

Bombyx mandarina (Lepidoptera: Bombycidae), the presumed ancestor of B. mori, has long been a subject of study to illustrate the geographic relationships in connection with origin of B. mori. We report 97 mitochondrial genome (mitogenome) sequences of B. mandarina collected from Korea and Japan. Phylogenetic and population genetic analyses showed that all individuals of B. mandarina collected in Korean localities formed a strong group together with all individuals originated from northern China (mainly north of the Qinling-Huaihe line) and some of southern China. This group was placed as the sister group to B. mori strians suggesting that this group had been served as an immediate progenitor for B. mori.

Immune priming is an increased immunity after prior exposure to a specific pathogen as a kind of adaptive immunity and occurs in insects. However, its underlying mechanism is elusive in insects. Immune priming was detected in a lepidopteran insect, Spodoptera exigua. Prior infection with a heat-killed pathogenic bacterium, Xenorhabdus hominickii, increased survival upon the second infection of the live bacteria compared to larvae without pre-exposure. Plasma collected from larvae with the prior infection significantly up-regulated cellular and humoral immune responses compared to the similar treatment without prior exposure. However, when the active plasma exhibiting immune priming was heat-treated, it lost the priming activity, suggesting a presence of protein factor(s) in the immune priming. Lipocalin is a lipid carrier protein and is well known in vertebrates for diverse physiological functions including immunity. An apolipoprotein D3 (ApoD3) is known to be a lipocalin functioning in immune priming in a mosquito, Anopheles gambiae. A homologous ApoD3 (Se-ApoD3) was identified in S. exigua. Se-ApoD3 was expressed in all developmental stages and larvae, it was highly expressed in hemocytes. RNA interference (RNAi) of Se-ApoD3 expression was performed by injecting its specific dsRNA. The larvae treated with the RNAi were impaired in cellular and humoral immune responses. Furthermore, the plasma collected from RNAi-treated larvae lost the immune priming even at the prior exposure. These suggest that Se-ApoD3 mediates the immune priming in S. exigua.

The soluble epoxide hydrolase (sEH) plays a crucial role in insect immunity and development by metabolizing oxylipins such as EpOMEs and EETs. This study investigates sEH's involvement in insect antiviral response against Autographacalifornicanuclear polyhedrosis virus (AcNPV) infection. Viral infection assays were performed on Plutellaxylostellaand Marucavitrata, utilizing occlusion bodies (OB, via feeding) and budded virus (BV, through injection). Insect mortality was monitored every 12 h for up to 7 days. Our findings demonstrated a dose-dependent impact of both virus forms (OB and BV) on insects. Additionally, the sEH inhibitor, AUDA (12-(3-adamantan-1-yl-ureido) dodecanoic acid), was employed alongside the virus. The results indicated that combining AUDA with the virus increased insect mortality. Furthermore, fluorescence assays revealed the gradual movement of the virus from the gut to hemolymph and fat body. AUDA was observed to expedite virus infection. Moreover, sEH expression rapidly increased along with the viral infection in Spodoptera exigua. RNA interference of sEH expression enhanced the viral virulence against S.exigua. These suggest that EpOMEs play crucial roles in immune resolution against viral infection in insects.

Family Nolidae has not much been treated as noticeable pest. In other countries, there are some pest species like Garella musculana (Erschoff, 1874), Uraba lugens Walker, 1866, and Earias insulana (Boisduval, 1833), but those are not reported in Korea. Recently, the occurrence of a nolid species, Meganola major (Hampson, 1894) has been increasing, especially in Jeollanam-do Prov. This species is mainly distributed in Indian subregion and Southeast Asia, but also East Asia reported, but until now, there are no outbreak information. Neverthereless, recent outbreak might be lead by global warming, and their growth stage. This seems to be evidence of the potential outbreak risk of nolid moths, but unfortunately, their ecology is not mostly well-studied. In this discussion, we are focusing on the potential outbreak of nolid moths in East Asiatic and Oriental regions.

Background: Outcome measures (OMs) are essential components of evidence- based practice as they help in implementing interventions and assessing changes in a patient's status before and after treatment. However, many therapists do not utilize OMs in their practice, and research on the factors that influence physical therapists' decisions regarding the use of OMs primarily consists of quantitative or survey-based studies. Objectives: The purpose of this study was to select research studies on OMs use among physical therapists through a systematic review, identify questionnaire characteristics, and assess the quality of items collection and research. Design: A systematic review. Methods: This study systematically collected survey studies on the use of OMs from five databases (KMBase, RISS, KCI, Pubmed, and Cochrane) based on the criteria of PRISMA's systematic review flowchart. The quality of the studies was assessed using the AXIS Tool. Results: A total of eight studies were selected. In most of the studies, the questionnaires were developed internally, validated through preliminary research, and reliability testing was not conducted. The items used within OMs were divided into 10 categories, with the highest frequency of questions centering around how to use OMs, and the lowest frequency regarding work environment and reasons. All eight studies were determined to be of good quality. Conclusion: The collected OMs questionnaires revealed variations in the item frequency. Future qualitative research and participant feedback are needed to develop a consistent questionnaire and validate its validity and reliability.

목적: 본 연구는 COVID-19의 대유행 동안 20~30대 성인을 대상으로 컴퓨터시각증후군 (CVS, computer vision syndrome) 관련 눈의 자각증상과 시력 관련 불편감을 파악하고, 눈 건강을 위해 사용하는 예방법을 알아보고자 하였다. 방법 : 2021년 12월부터 2022년 2월까지 안경 또는 콘택트렌즈 처방과 상담을 위해 일개 안경원에 내원 했던 20~30대 성인 중에서 전신질환 및 안질환, 안과적 수술 경험이 없는 150명을 대상으로 하였다. 연구도구는 대상자의 일반적인 특성(3문항), CVS 자각증상(16문항), 시력 관리(2문항), 눈의 조절기능 이상(2문항), 청색광 차단 안경사용(2문항), 근거리교정(1문항)으로 총 26문항으로 구성된 설문지를 이용하였다. 결과 : 전체 대상자들의 CVS 자각증상은 “눈물이 많이 발생한다”가 109명(72.7%)으로 가장 높은 빈도를 보였 고, 그다음은 “눈이 아프다”가 104명(69.3%)으로 나타났다. CVS 자각증상 중 시력 관련 자각증상은 “사물이 흐 려보인다”가 106명(70.7%)으로 가장 많았다. 시력 관리방법으로는 “시력의 저하를 느껴서”가 105명(70.0%)으로 가장 많았고, 청색광 차단 안경 사용하는 대상자는 150명(84.7%)으로 80% 이상의 대상자들이 기능을 넣어서 사 용하고 있다. 결론 : CVS 자각증상 16문항 중 14문항에서 50% 이상으로 나타났다. 청색광 차단 안경을 사용하지 않는 대상 자는 CVS 자각증상 중 시력과 관련된 “시력이 나빠지는 것 같다”가 69.6%로 가장 높게 나타났다. COVID-19 대 유행 동안 자각적인 불편증상과 시력저하를 호소하는 대상자가 높게 나타나고 있다. 따라서 안경처방 시 생활환 경, 사용시간 및 자각적 증상에 대하여 문진이 필요하다고 생각된다.

At a time when the demand for drones is increasing, a plan to utilize drone images was sought for efficient promotion of cadastral resurvey. To achieve the purpose of this study, the technical and legal status of drone images was reviewed, and through this, the possibility of using it for cadastral resurvey was primarily reviewed. subsequently, an experiment was conducted targeting the project district to examine whether drone images were applied to boundary extraction, which is the primary process of cadastral resurvey. As a result of the experiment, it was found that boundary extraction from images is possible. However, in some cases, it is impossible due to field conditions or image quality. Therefore, it is necessary first to apply cases where boundary extraction is possible to cadastral resurvey and seek solutions for some impossible cases. In particular, the image quality problem may have problems with the current technology, but it will also have problems with the existing drone equipment. So, standard for drone calibration should also be established. Finally, the cadastral resurvey surveying procedure using drones was also presented.

In 2022, research for native prokaryotic species in Korea reported 10 unrecorded bacterial strains affiliated to phyla Actinomycetota, Bacillota, and Pseudomonadota. The strains formed monophyletic clades with the most closely related species (with ≥98.7% sequence similarity) in the 16S rRNA gene sequencing. Among them, four species of the phylum Actinomycetota, two species of the phylum Bacillota, and four species of the phylum Pseudomonadota have not been reported in Korea, suggesting unrecorded species in Korea. Information on strains such as Gram staining reaction, colony and cell morphology, biochemical characteristics, and isolation sources were provided in the species description.