CACTA is a class 2 transposon superfamily found exclusively in plants. In this study, we have applied CACTA-TD technique to study the genetic diversity among 90 waxy corn inbred lines. While CACTA-TD fragments ranged approximately from 100 to 650 bp in si

This study was carried out to compare the major volatile components in essential oil from different origin of Atractylodes spp. which is being traded as a crude herbal drug in Korean herbal markets. From the two Atractylodes of major volatile components were similarly detected such as the β-selinene, β-sesquiphellandrene, germacrene B, 2,7-dimethoxy-2-methylnaphthalene and 9-methoxy-2,3-dihydrofuro3,2-qcoumarin. Among the volatile components, the major components were 2,7-dimethoxy-2-methylnaphthalene (40.98%), 9-methoxy-2,3-dihydrofuro 3,2-q coumarin (15.74%), and β-sesquiphellandrene (1.98%) in both Atractylodes. As a results, It was found that the two Atractylodes were the same species which was being traded in the Korean herbal markets as the A. japonica. not to different species of A. japonica and A. macrocephalla, respectively.

The aims of this study are 1) to test oocytes and embryos collected from in-vivo and in-vitro to achieving the valuable protocol by culturing, vitrifying and thawing of oocytes/embryos, and 2) to transfer them to recipient, and finally have resulted in pregnancies from recipient females after surgical or nonsurgical transfer. In vitro maturation and fertilization were performed according to the procedures of Funahashi et al. Fertilized oocytes were cultured in glucose-free NCSU 23 supplemented with 5 mM sodium pyruvate, 0.5 mM sodium lactate and 4 mg/ml bovine serum albumin for 2 days at 39, and 10% fetal bovine serum was added to the culture medium thereafter. Embryos were treated with 7.5g/ml cytochalasin-B for 30 min, centrifuged at 13,000 g for 13 min and then exposed sequentially to an ethylene glycol (EG) vitrification solution, aspirated into OPSs, and plunged/thawed into/from liquid nitrogen. In vivo embryos were surgically collected from three donors after Al. Forty-six embryos (18, 9 and 19 embryos, respectively) were washed 3 times in mPBS+10%FBS, followed treatments : cultured, centrifuged, vitrified, recovered and transferred to recipients as in vitro prepared embryos. Three recipients received surgically 34(control), 188 and 184 embryos (derived from abattoir), respectively. Another three recipients were received nonsurgically 150, 100 and 150 embryos, respectively. All recipient sows exhibited delayed returns to estrus. To our knowledge, these results suggest that required an improved techniques, more vigorous embryos preparation and cleaner uterous condition(use gilt).

The objective of this study was to evaluate the effect of GnRH or estradiol in a CIDR-based timed Al (TAI) protocol on follicular turnover, synchronized ovulation and pregnancy rates in Holstein cows. Cows were treated at random stages of the estrus cycle with an insertion of an intravigal progesterone (1.9 g) device (CIDR, Day 0) and either no other treatment (control group; n=10), injection of 100 ug fertirelin acetate (GnRH group; n=10) or 4 mg estradiol benzoate (estradiol group; n=10). Seven days later devices were removed and an injection of 25 mg PGF2a was administered. On Day 9, 100 ug GnRH was administered. Cows received a fixed-time insemination 16 h after injection of the GnRH.

A new covered barley variety, 'Daeyeonbori' was developed from the cross between Milyangketbori, a stabile for machine harvest, and Olbori by the barley breeding team in the National Yeongnam Agricultural Experiment Station (NYAES) in 1999. An elite line,

MITEs (miniature inverted-repeat transposable elements) are one of the major transposable elements in maize. We have applied MITE-AFLP technique to study the genetic diversity and genetic relationship among 25 elite maize inbred lines. While MITE-AFLP fra

The callus from the hypocotyl region of immature embryo of Citrus junos Sieb. was efficiently induced in the ½ MS medium containing 45uM BA after 8 weeks culture. The callus was developed into the two callus type, embryogenic callus and nonembryogenic callus, which can be distinguished by visual examination depending on color and appearence. In vitro regeneration of callus established efficiently in the hormone-free MS medium from the embryogenic callus. In order to investigate the physiological changes depending on the developmental stage of embryo, the embryo was formed in the MS medium. The embryogenic and nonembryogenic callus, and the various stages of the somatic embryo were examimed the changes of esterase activity, and their isozyme patterns as well. The protein content and esterase activities was gradually increased on the developmental stages of embryo. Total protein pattern were different by the SDS-PAGE and were appeared strong band of 23 KD in the torpedo stage. The pattern of the esterase isozyme was exhibited a difference between embryogenic callus and nonembryogenic callus. It was appered pI 6.0, 8.0, 8.2 in the embryogenic callus. Also the new band of pI 4.75 was appeared in the cotyledon. These results suggest that the changes of esterase activities and isozyme patterns are importent factor in the differentiation and development of citrus.