장미는 소비자 기호도가 빠르게 변화하는 화종으로서 소비 트렌드 변화에 대응하고 로열티 지출 부담을 줄이고자 전북농업기술원에서 향기가 있고 수량성이 우수한 절화용 복색 스탠다드 ‘Blondie’를 육성하였다. 2013년 ‘Regato’를 모본으로, ‘Cherry Brandy’를 부본으로 인공교배를 실시한 후 74개 종자를 획득하여 53 실생 개체를 얻었다. 2014~2015년 특성 평가를 통해 개화가 균일하고 향기가 있으며 생산성이 우수한 ‘R132093-45’ 계통을 선발하였고, 2016년부터 2년 동안 특성검정, 기호도 평가 및 생산력 검정을 통하여 ‘Blondie’ 품종으로 육성하였다. ‘Blondie’는 오렌지와 살구색 복색 화색(O24C, R36D)의 겹꽃 스탠다드 계열로 향기가 있고, 신초는 안토시아닌 색소가 강하게 발현되며, 엽 형태는 중간 타원형, 엽색은 진한 녹색(N137A), 잎 표면의 광택은 강하다. 꽃 모양은 불규 칙한 원형이고 꽃 측면에서 본 윗부분과 아랫부분은 모두 평평한 고유특성을 가진다. ‘Blondie’의 소화폭은 9.4cm로 대조 품종(‘Hera’)보다 작고 꽃잎수도 36.7개로 적었으며, 가시 발생 정도, 절화장, 경경 및 엽 생육은 유사하였다. ‘Blondie’의 절화 수명과 기호도 평가는 대조품종인 ‘Hera’보다 우수하였으며, ㎡당 연평균 절화량은 150.3본으로 대조품종보다 18.4본 더 많았다. ‘Blondie’ 품종은 2019년 7월 22일 신품종보호권이 최종 등록(등록번호: 제7824호)되었다.

It is known that the growth and development of the mosquito are greatly affected by the change of the meteorological factors. In particular, temperature and precipitation are closely related to the life cycle of the mosquito, and their effects have different characteristics for each species of mosquito. Therefore, to develop a mosquito activity index based on mosquito density, it is essential to develop a prediction model based on weather data. In this study, we developed a functional formula that can estimate the change of mosquito density according to the change of meteorological factors using the mosquito classification data of Incheon region collected from 2011 to 2017. Also, using the data of the digital mosquito monitoring system (DMS) from April to October 2018, mosquito activity index according to characteristics of space in city was developed. In order to reflect the temporal characteristics of the mosquito life-cycle, we used data of temperature and precipitation prior to 1-2 weeks, and used land cover data to reflect the spatial characteristics of mosquito density. Density of Culex pipiens collected in the Incheon area were gradually increased when the average temperature increased two weeks ago after adjusting the precipitation. However, when the average temperature reached 22°C, the density of Culex pipiens marked a peak, and above the 22°C, the density was decreased. The predicted mosquito activity index calculated by applying the machine learning method to the DMS data of the Incheon area is designed to calculate from 1 to 10 grades. The accuracy of the mosquito activity index was 87% when the one grade error was allowed.

The purpose of this study is to confirm whether spontaneous adipocyte generation during chondrogenic induction culture affects the chondrogenic differentiation of porcine skin-derived stem cells (pSSCs). For this purpose, chondrogenic differentiation characteristics and specific marker gene expression were analyzed using cell lines showing different characteristics of spontaneous adipocyte formation. Of the four different lines of pSSCs, the pSSCs-IV line showed higher Oil red O (ORO) and glycosaminoglycan (GAG) extraction levels. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis revealed that the levels of adipogenic markers peroxisome proliferator-activated receptor gamma 2 (PPARγ2) and adipocyte Protein 2 (aP2) mRNAs were significantly higher in pSSCs-IV than those of the other pSSC lines (P<0.05). Among three chondrogenic markers, collagen type II (Col II) and sex determining region Y-box (Sox9) mRNAs were strongly expressed in pSSCs-IV (P<0.05), but not in aggrecan (Agg), which was significantly higher in pSSCs-II (P<0.05). These results demonstrate that the spontaneous adipocyte generation during chondrogenic differentiation has a positive effect on the chondrogenesis of pSSCs. More research is needed on the correlation between adipocyte generation and cartilage formation.