우라늄 변환시설 가동 중 발생하여 라군(lagoon)에 저장중인 방사성 슬러지 폐기물에 대한 처리는 시설 해체과정에서 매우 중요한 업무 중 하나이다. 슬러지 구성성분 중 다량을 차지하는 질산암모늄의 폭발 위험성 등으로 인해 미생물을 이용한 질산염의 분해는 질산염을 안정적으로 처리할 수 있는 효과적인 방법이라 할 수 있다. 본 연구에서는 라군 슬러지의 약 60 wt%를 차지하는 질산염을 혐기성 균주의 하나인 Pseudomonas halodenidificans를 이용하여 탈질하기위한 공정 변수에 대한 영향을 평가하였다. 온도, 질산염 농도, 전자공여체의 영향, C/N 비율, 초기 접종하는 균주의 비율, pH등의 공정변수에 대하여 실험한 이번 결과는 향후 연속식 공정 설계를 위한 기초 자료로 사용될 것이다.

우라늄 변환시설의 라군 슬러지에 함유된 질산염의 안정적 처리를 위해 물 첨가 용해를 실시한 뒤, 여과 케이크의 안정화 특성에 대하여 알아보았다. 물 용해에 의해 대부분의 질산염은 고농도 질산염 용액으로 제거되었으므로, 여과 케이크의 열분해는 에서 하나의 단계로 수행하였다. Muffle furnace를 이용하여 에서 5시간동안 여과 케이크의 열분해를 실시한 결과 라군 1 슬러지에 포함된 U은 의 열분해와 함께 의 형태로 안정화 되었다. 라군 2 열분해 잔류물의 경우에는 열분해 시 생성된 CaO가 냉각과정에서 수분과 반응하여 로 전환되는 것을 TG/DTA 분석과 XRD 분석을 통해 확인할 수 있었지만, 처분장에서 대기중 노출이나 지하수의 침출 등에는 안정한 화합물로 알려져 있으므로, 특별한 첨가제의 첨가 없이 단순 열분해 후 처분이 가능할 것으로 판단된다.

Steel structures with added viscoelastic dampers are analysed to investigat their behavior under earthquake excitation. The direct integration method, which produces exact solution for the non-proportional or non-classical damping system, is used throughout the analysis. The results from modal strain energy method are also provided for comparison. Then a new analytical a, pp.oach, based on the rigid floor diaphragm assumption and matrix condensation technique, is introduced, and the results are compared with those obtained from direct integration method and modal strain energy method. The well known phenomenon, that the effectiveness of the viscoelastic dampers depends greatly on the location of the dampers, is once again confirmed in the analysis. It is also found that the modal strain energy method generaly underestimates the responses obtained from the direct integration method, especially when the dampers are placed in only a part of the building. The proposed method turns out to be very efficient with considerable saving in computation this and reasonably accurate considering the reduced degrees of freedom.

Photocatalytic green energy H2 production utilizing inexhaustible solar energy has been considered as a potential solution to problems of energy scarcity and environmental contamination. However, the design of a cost-effective photocatalyst using simple synthesis methodology is still a grand challenge. Herein, a low-cost transition metal, Cu-loaded one-dimensional TiO2 nanorods (Cu/TNR) were fabricated using an easy-to-use synthesis methodology for significant H2 production under simulated solar light. X-ray photoelectron spectral studies and electron microscopy measurements provide evidence to support the successful formation of the Cu/TNR catalyst under our experimental conditions. UV-vis DRS studies further demonstrate that introducing Cu on the surface of TNR substantially increases light absorption in the visible range. Notably, the Cu/TNR catalyst with optimum Cu content, achieved a remarkable H2 production with a yield of 39,239 μmol/g after 3 h of solar light illumination, representing 7.4- and 27.7-fold enhancements against TNR and commercial P25, respectively. The notably improved H2 evolution activity of the target Cu/TNR catalyst was primarily attributed to its excellent separation and efficiently hampered recombination of photoexcited electron-hole pairs. The Cu/TNR catalyst is, therefore, a potential candidate for photocatalytic green energy applications.

Honey used as conventional medicine has various pharmacological properties. In the honey and anti-inflammatory effect, Gelam honey and Manuka honey has been reported to exert anti-inflammatory activity. However, the anti-inflammatory effect and potential mechanisms of acacia honey (AH) are not well understood. In this study, we investigated anti-inflammatory activity and mechanism of action of AH in LPS-stimulated RAW264.7 cells. AH attenuated NO production through inhibition of iNOS expression in LPS-stimulated RAW264.7 cells. AH also decreased the expressions of IL-1β, IL-6 and TNF-α as pro-inflammatory cytokines, and MCP-1 expression as a pro-inflammatory chemokine. In the elucidation of the molecular mechanisms, AH decreased LPS-mediated IκB-α degradation and subsequent nuclear accumulation of p65, which resulted in the inhibition of NF-κB activation in RAW264.7 cells. AH dose-dependently suppressed LPS-mediated phosphorylation of ERK1/2 and p38 in RAW264.7 cells. In addition, AH significantly inhibited ATF2 phosphorylation and nuclear accumulation of ATF2 in LPS-stimulated RAW264.7 cells. These results suggest that AH has an anti-inflammatory effect, inhibiting the production of pro-inflammatory mediators such as NO, iNOS, TNF-α, IL-6, IL-1β and MCP-1 via interruption of the NF-κB and MAPK/ATF2 signaling pathways.

Background : The most problematic disease in Boxthorn is Anthracnose. New variety with Anthracnose-resistant is good for high yield and Safe fruit production in open field. Therefore it is necessary to develop a New variety with Anthracnose -resistant and high yield. Methods and Results : The new boxthorn line, CB10511-104 was selected from the cross between Cheongun (IT232599) and CB08456-113 to breed new variety with high yield and Anthracnose-resistant in 2011. Its preliminary yield test was performed from 2012 to 2013 and the selected line was named Cheongyang 25. Its regional yield trials were carried out in Cheongyang, Yesan and Jindo for 3 years, from 2014 to 2016. Cheongyang 25 was registered as the new variety, Cheonggeum, in 2016. The specific characteristics were summarized as follows; Tree shape is open type and the leaf is round-lanceolate. The flowers are normal size and purple. The fruit is large size, round oval and yellowish red. The flowering was June 19 with medium flowering. Number of sprout branch by pruning was generated more than the check variety, Cheongmeong. The infection rates on leaves to Eriophys macrodonis Keifer was as strong as 39.6 percent. Anthracnose on fruits in open field was slightly similare compared to the check variety, but check variety was Anthracnose-resistant. The content of betaine and free sugar in dried-fruits were higher than that of the check variety. The dried-fruit yield was decreased about 17 percent in open field. Conclusion : This variety ‘Cheonggeum’ was suitable for cultivation in open field because of Anthracnose-resistant. It is necessary the companion variety, Cheonghong, because of self-incompatible.

Background : This study was conducted to examine the suitability of the blue/white vinyl for ginseng cultivation greenhouses in different regions in an effort to develop a stable ginseng cultivation technology and contribute to popularizing ginseng greenhouse farming. Methods and Results : For comparison purposes, ginseng plants were cultivated in the traditional shading and greenhouse shading facilities. The average temperature in the greenhouse shading facilities during the period of May through October was 20.5℃ in Namwon, 21.4℃ in Jinan, and 23.3℃ in Iksan, with Iksan recording a 1.9–2.8℃ higher average temperature, presumably due to its location in a plain area with low elevation. The average temperatures in the traditional shading facilities ranged between 22.9℃ and 25.7℃, 2.4–2.6℃ higher compared with the greenhouse shading facilities. As for the incidence of disease and pest, as low as 0.1–0.3% of the plants grown under greenhouse shading were infected with Colletotrichum dematium, Alternaria panax, and Rhizoctonia solani, whereas much higher incidences were noted in the control plants, with 3.3–11.3%, 4.7–17.0%, 0.1–0.5%, and 3.0–5.7% infected with Colletotrichum dematium, Alternaria panax, Rhizoctonia solani, and Phytophthora cactorum, respectively, throughout the regions. Heat injury occurred only in Iksan (0.3%) in the case of greenhouse-shaded plants, whereas the control plants suffered from much severer heat injury: 3.5% in Namwon, 10% in Jinan, and as high as 35.3% in Iksan. As for the roots, Namwon outperformed other regions in root length (29.7 ㎝), taproot length (8.7 ㎝), and taproot diameter (25.3 ㎜), and also the root fresh weight (53.4 g), followed by Jinan (48.7 g) and Iksan (40.4 g). In yield as well, the greenhouse in Namwon outperformed other regions with 1,297㎏/10a, followed by Jinan (1,183 ㎏) and Iksan (932 ㎏). The effect of greenhouse blue-white vinyl shading on yield by region was analyzed to increase of 177% in Namwon, 209% in Jinan, and 173% in Iksan with respect to their respective traditional shading facilities. Conclusion : The suitability of the blue/white vinyl shading in ginseng cultivation greenhouses was tested experimentally in three different regions. As a result, greenhouse-shaded ginseng plants were found to be much less affected by heat injury compared with traditionally shaded ginseng plants, resulting in much higher yields in all regions. This allows the conclusion that greenhouse ginseng cultivation can contribute to more stable ginseng yield and popularization of ginseng cultivation.

Background : This study was conducted to determine the optimal ginseng greenhouse shading material for minimizing heat injury and maximizing the yield of ginseng plants in an effort to develop a stable ginseng cultivation technology and contribute to popularizing ginseng greenhouse farming. Methods and Results : Three different types of greenhouse shading material were used: polyethylene (PE) film + black screen (95%), light scattering film + black screen (75%), and blue-white vinyl. Important experimental details were implemented in accordance with the Analysis Standard of Agricultural Test and Research set out by the Rural Development Administration. The average greenhouse temperature for the period of May through October was 20.0℃ under the PE film + black screen, 20.2℃under the light scattering film + black screen, and 19.7℃ under the blue/white vinyl. High average photosynthesis rates of ginseng plants were demonstrated by light scattering film+black screen (2.35 μmole CO2/㎡/s) and blue-white vinyl (2.38 μmole CO2/㎡/s), with the PE film + black screen showing a considerably lower photosynthesis rate (1.55 μmole CO2/㎡/s). Heat injury occurred in 0.3%, 10%, and 0.1% of the plants grown under the PE film + black screen, light scattering film + black screen, and blue-white vinyl, respectively. As for the roots, the blue-white vinyl outperformed the other shading materials in root length (25.7 ㎝), taproot length (5.9 ㎝), and the number of fine roots (23.2). The blue-hite vinyl also yielded the greatest root fresh weight (30.2 g), followed by the light scattering film + black screen (29.1 g), with the PE + black screen yielding by far inferior average weight (22.0 g). The highest crude saponin content was yielded by the light scattering film+black screen (16.61 ㎎/g), followed by blue-white vinyl (11.36 ㎎/g) and PE + black screen (11.0 ㎎/g). Conclusion : Comparing the effects of three different shading materials on greenhouse-cultivated 3-year-old ginseng plants, the blue/white vinyl outperformed two other shading materials in most of the growth characteristics, whereas the and the light scattering film + black screen yielded the highest ginsenoside content.

Background : In recent, a nationwide decrease in ginseng cultivation area and production is observed, and as the sales of ginseng decreases due to the economic downturn, the ginseng industry in Korea is going through more and more difficulties. However, sales of young ginseng, which are ready for the market after a short-term cultivation - 25 to 45 days - of 1 year old ginseng seedlings or 2 - 3 year old young ginseng in greenhouses and plant factories without using any chemical fertilizers, in large grocery chains and online shops is increasing. However, proper studies on cultivation of young ginseng in greenhouses are yet to be conducted, and thus, experiencing several issues including the cost burden of bed soil and disease occurred from reuse of the soil. Therefore, this study is to provide solutions for farmers cultivating young ginseng by determining the optimal type and amount of bed soil for box culture. Methods and Results : To determine the optimal type of bed soil for cultivation of young ginseng, six types of bed soil were produced by blending bed soil specialized for ginseng, granite residual soil and leaf mold in different ratios. To determine the optimal amount of bed soil for box culture of young ginseng, 1 year old ginseng seedlings are planted in three boxes - each in size of 52 ㎝ × 36.5 ㎝ × 9 ㎝ (W × L × H) filled with soils of 6 ℓ, 7 ℓ and 8 ℓ for an experimentation. Growth and development of 45-day old young ginseng showed satisfactory progress in the following order: 100% commercially available bed soil > 80% commercially available bed soil + 20% granite residual soil > 60% commercially available bed soil + 20% granite residual soil + 20% leaf mold, while the gross weight of below-aerial parts showed the same result. Conclusion : The optimum type of bed soil for greenhouse cultivation of young ginseng is the commercially available bed soil specialized for ginseng cultivation. However, the young ginseng showed relatively satisfactory growth and development in mixed bed soil - 60% commercially available bed soil + 20% granite residual soil + 20% leaf mold - and the growth and development of young ginseng were most satisfactory when the box (52 ㎝ × 36.5 ㎝ × 9 ㎝, W × L × H) was filled with 8 ℓ bed soil.

Background : With the recent increase in the area of ginseng cultivation in greenhouses, demand for studies of proper cropping patterns in direct seedling of ginseng and varieties of cultivation are increasing, and the needs for clean production of quality ginseng in greenhouses are emerging in connection with the consumer demand for pesticide-free ginseng and the supply and demand of ginseng seedling for cultivation of young ginseng. Therefore, this study is to provide baseline data in improving of quality of ginseng cultivated in greenhouses by examining the contents of ginsenoside in ginseng cultivated in different cropping patterns and different varieties. Methods and Results : As a result of examining the contents of ginsenoside in 3-year old, direct seedling ginseng cultivated in greenhouses in different depths – 10 ㎝, 20 ㎝ and 30 ㎝ - and width – 90 ㎝, 100 ㎝, 110 ㎝ and 120 ㎝ - of furrows, the contents of ginsenoside showed little differences by the depths of furrow in the furrow width of 90 ㎝ but, the highest content of ginsenoside Rb1 was observed in the furrow width of 120 ㎝ and depth of 20 ㎝, while the ginsenoside contents were high in the furrow depth of 20 ㎝ and width of 100 ㎝ and 120 ㎝. The contents of ginsenoside in different varieties of direct seedling cultivation in greenhouses were greater in the following order: Geumgin > Geumsun > Chunpoong, while the contents of ginsenoside Rb1 were observed to be superior in Sunhyang, Gopoong and Geumseon and the contents of ginsenoside Rb2 were greater in the order of Geumgin > Guemsun >　Cheonpoong. Conclusion : Based on the results, the contents of ginsenoside in different cropping patterns of direct seedling cultivation in greenhouses were higher in lower depths of furrow - 10 ㎝ and 20 ㎝ - at furrow widths wider than 100 ㎝. The contents of ginsenoside in different varieties of direct seedling cultivation in greenhouses were higher in varieties of Geumjin, Geumsun and Chunpoong.

Background : Schizandra chinensis Baillon have five tastes and lately it is using a beverage broadly. Schizandra chinensis is one of the top producing medicinal plant in Korea. Mungyeong of Gyongbuk province produce almost of Schizandra chinensis. Maturity of Schizandra chinensis get 3 years and proliferation of Schizandra chinensis was not a manual. It is needed that a new cultivar has a big fruit and high quality chracteristics using processed food and beverage. Methods and Results : 105 lines of Schizandra chinensis were collected on Mungyeong, Yeongwol, Jinan. It were studied it’ characteristics especially it’s fruit trait. Fruit traits of Schizandra chinensis were researched on fruit length, fruit weight, maturity, number of fruit, male and female ratio, powdery mildew. Fruit length of Schizandra chinensis is relation of fruit weight. It were founded 15 lines of long fruit length. 5 lines were studied high fruit weight and it’s weight were 32 to 41g. Number of fruit has relation with fruit weight and high fruit weight gets many fruits. it’s numer of fruits were 3 to 41. Male and female ratio were very impotant characteristic. High level of female ratio has quantity of fruit. High level of female ratio were founded 2 lines. Finally It was selected 3 good breed lines of Schizandra chinensis. Conclusion : 105 lines of Schizandra chinensis Baillons were collected on Mungyeong, Yeongwol, Jinan. It were founded 15 lines of long fruit length and 5 lines were studied high fruit weight. High level of female ratio were founded 2 lines. 3 good breed lines of Schizandra chinensis were selected.

Background : As the farmers who grow Chinese matrimony vine (Lycium chinense Miller) in rain-shelter greenhouses increase, it is necessary to develop a cultivar with self-compatibility and high quality. Methods and Results : The new boxthorn line, CBP06419-243 was selected from the cross between CBP03310-250(IT232705) and CBP05399-1 to breed the cultivar with self-compatibility and high quality. These lines used for crossing was tetradilpoid induced by the colchicine treatment on bud. Its preliminary yield test was performed from 2009 to 2011 and the selected line was named Cheongyang 18. Its regional yield trials were carried out in Cheongyang, Yesan and Jindo from 2012, 2014, 2015 and Cheongyang 18 was registered as the new variety, Whasu, in 2015. The specific characteristics were summarized as follows; Tree shape is semi-open type and the leaf is lanceolate. The flowers are distinctly larger. The fruit type is medium size, long oblong and yellowish red. The flowering was June 21 with medium flowering. Branching by pruning its sprout was generated less than the check variety, Cheongmeong. The infection rates on leaves to Eriophys macrodonis Keifer was as strong as 7.7 percent. Anthracnose on fruits in open field was slightly severe compared to the check variety, but the infection rates in rain shelter greenhouses was similar. The content of betaine and free sugar in dried-fruits were higher than that of the check. The dried-fruit yield was decreased about 13 percent in open field, but increased 18 percent in rain shelter greenhouses. Conclusion : This variety ‘Whasu’ was suitable for cultivation in plastic film greenhouses because of self-compatibility and high-yielding. It is not necessary the companion variety.

Background : For increasing saponin content of ginseng cultivated in shaded plastic house, this research was performed to investigate growth characteristics and saponin content of ginseng according to foliar spray of chitosan and water-soluble silicates processing. Methods and Results : The ginseng cultivars to be used this research were Cheonpung, Yeonpung and 1-year-old seedlings of Cheonpung, Yeonpung was transplanted on March 24, 2015. Planting density was 72 plant/3.3㎡and Shading material of plastic house used blue-white shading vinyl. The processing method of organic matter is as follows, we were applied chitosan (40 kg / 10a), silicate (3kg / 10a) to soil scheduled for ginseng cultivation with basal application. Then, we were diluted with chitosan and silicates to 1000-fold and investigated growth characteristics, saponin content of ginseng after foliar spray twice a month from May to September. Conclusion : Photosynthetic rate of ginseng according to treatment of organic matter was higher in the chitosan experimental group compared to the control group and photosynthetic rate of ginseng cultivars was the highest in Cheonpung’s silicate group(3.70 μmole CO2/㎡/s). The growth characteristics in above-ground part of 2-year-old ginseng was a good in the organic matter treatments compared to the control group. Above-ground part’s growth characteristics of ginseng according to treatment of organic matter showed a good trend in plant height, stem length, stem diameter of chitosan experimental group. The growth characteristics in under-ground part of 2-year-old ginseng was a good in the organic experimental groups. In Yeonpung of the chitosan experimental group, fresh root weight per plant was 9.2 g/plant that increased by 46 % compared to the control group. In Cheonpung of the silicate experimental group, fresh root weight per plant was 8.3 g/plant that increased by 56 % compared to the control group. In the chitosan experimental group, crude saponin content of under-ground part was Cheonpung(10.38 ㎎/g), Yeonpung(12.29 ㎎/g), control(11.53 ㎎/g), respectively. In the silicate experimental group, crude saponin content of under-ground part was Cheonpung(11.73 ㎎/g), Yeonpung(14.21 ㎎/g), respectively. Crude saponin content of ginseng according to treatment of organic matter was. generally higher in Yeonpung than Cheonpung.

Background : For increasing saponin content of ginseng cultivated in shaded plastic house, this study was performed to investigate growth characteristics and saponin content of ginseng (Panax ginseng C.A. Mayer) according to foliar spray of germanium and water-soluble silicates processing. Methods and Results : Used a native species in this study is violet-stem variant that most commonly cultivated in ginseng’s farms. 1-year-old violet-stem variant was transplanted on March 24, 2015 and planting distance was 11 × 20 cm. Shading material of plastic house was used blue-white shading vinyl. The processing method of inorganic dissolved matter is as follows, we were diluted with germanium and water-soluble silicates to 500-fold, 1000-fold and investigated after foliar spray twice a month from May to September. The growth characteristics in above-ground part of 2-year-old ginseng was a good in the inorganic matters treatment compared to the control treatment, the difference of above-ground growth characteristics between the inorganic dissolved matters treatments was not significant. The growth characteristics in under-ground part of 2-year-old ginseng was a good in the inorganic matter treatments and root weight per plant in the 500-fold dilution of germanium was 12.4 g that increased by 29 % compared to the control (9.6 g). Crude saponin content of under-ground part was higher generally in inorganic matter treatments compared to the control (11.53 ㎎/g). In the 1000-fold dilution of water-soluble silicate and germanium (50:50), crude saponin content was the highest in 12.91 ㎎/g. Crude saponin content of above-ground part was higher generally in inorganic matter treatments compared to the control (61.76 ㎎/g). In the 1000-fold dilution of water-soluble silicate and germanium (50:50), crude saponin content was the highest in 65.69 ㎎/g. Conclusion : From the above results, we concluded that germanium and water-soluble silicates could be useful matters in promoting growth characteristics and saponin content of ginseng (Panax ginseng C.A. Mayer).

Background : This study was performed to set proper soil moisture tension for promoting seed emergence and yield of ginseng when direct seeding cultivation of ginseng was carried out in shaded plastic house. Methods and Results : The test cultivars were used Cheonpung, Yeonpung, Geumpung and seeds were sown on November 20, 2013. Irrigation starting point was set to 30, 40, 50 kPa and irrigation breakpoint was set to 20 kPa. Ginseng was cultivated in clay loam soil and shading material of plastic house were used blue-white shading vinyl. The emergence rate of 3-year-old ginseng cultivars in accordance with soil moisture tension indicated Cheonpung 97.0%, Yeonpung 95.0%, Geumpung 95.3% in each 50 kPa, 50 kPa, 30 kPa. In general, emergence rate of ginseng was higher tendency in 50 kPa (93.3∼97.0%). The average absolute soil moisture content during the growing season indicated a moisture content of 19.3% (30 kPa), 17.9% (40 kPa), 16.2% (50 kPa). Looking at the growth characteristics in above-ground part of 3-year-old ginseng cultivars according to soil moisture tension, Yeonpung, Cheonpung and Geumpung were good growth tendency in each 30kPa, 40kPa, 50kPa. On the other hand, looking at the growth characteristics in underground part of 3-year-old ginseng cultivars according to soil moisture tension, Cheonpung, Geumpung and Yeonpung were good growth tendency in each 30kPa, 40kPa, 50kPa. Yield per 10a was indicated that Yeonpung was the highest in the 50kPa to 456kg. Yield per 10a of Cheonpung and Geumpung indicated 347kg, 263kg in each 30kPa, 50kPa. Conclusion : From the above results, we concluded that ginseng(Panax ginseng C.A. Mayer) seems to be a difference of growth characteristics according to soil moisture tension. Therefore, it is need to manage soil moisture for each ginseng cultivars

In this study, genetic diversity of wild Codonopsis lanceolata collected in Korea were analysed using SSR makers. Wild C. lanceolata roots were collected in Jeollanam-do Jangheung-gun Choentae Mountain as in roots. The wild C. lanceolata plants were cultivated in Chungbuk National University greenhouse and the leaves were sampled from 36 plants. The genomic DNA of C. lanceolata was extracted using CTAB. PCR was performed using a program of 35 cycles at 94℃ for 30 sec, 60℃ for 30 sec, and 72℃ for 30 sec with an pre-denaturation of 94℃ for 5 min and a final extension of 72℃ for 30 min. The PCR reaction mixture contains 5 pmole of primers and 20 ng of DNA template in a 20 μL reaction volume. The genotype of the analyzed samples were very different. Therefore, the wild C. lanceolata collected in Korea look genetically diverse.

Codonopsis lanceolata is a perennial climber. The roots are used as medicinal materials or vegetables. Recently, demand for C. lanceolata is increasing as a healthy food. C. lanceolata is distributed in India and East Asia such as China, Japan as well as Korea. In South Korea, this plant is widely cultivated in Gangwon-do province. No C. lanceolata varieties were developed in Korea. The objective of this study is to analyze genetic diversity of C. lanceolata cultivated in Korea using SSR makers. C. lanceolata roots were collected in each region were cultivated in Chungbuk National University greenhouse. Samples were obtained from fresh leaves of 5 plants from each collection region. The genomic DNA was extracted using CTAB. Genetic diversity was analysed using 4 sets of C. lanceolata SSR makers. PCR was performed in total 20 μL reaction volume containing 20 ng of DNA template, 5 pmole of primers. The genotypes of the analyzed samples were very similar. That means that the genetic diversity of C. lanceolata cultivated in Korea is very low.