국제적으로 최근의 세계적인 축산 ICT 개발 추세는 음성, 맥박, 저작수, 호흡수, CCTV 영상인식 등 보다 다양하고 다중적ㆍ통합적인 센서기술을 접목하여 분만, 상해, 질병 감염, 발정, 반추 및 저작 등 가축의 중요한 생명ㆍ생산 활동을 판단하고 조기에 발견하는 연구들이 시도되고 있다. 이에 본 연구는 ICT 기술을 활용하여 농가 생산성 향상 방안의 일환으로 체류형 바이오캡슐 내 pH, 반추위 체온 및 활동성 센서를 활용한 생체정보를 수집하였고 도축등급과의 상관관계 여부를 파악하고자 수행하였다. 시험방법으로는 26개월령의 한우 거세우 10두를 공시하여 5개월간 바이오캡슐 자료들을 수집하였고 이후 도축하여 등급판정성적과의 연관성 여부를 분석하였다. 연구 결과 육량 등급과 관련이 있는 등심단면적, 등지방두께, 도체중과 생체형질인 반추위 pH, 체온 그리고 활동성과는 모두 정(+)의 상관을 보였다. 육질등급과 관련 있는 근내지방도 및 성숙도는 생체형질인 체온에서는 정(+)의 상관을, pH 및 활동성과는 부(-)의 상관을 나타낸 반면에, 조직감에 대하여 체온은 부(-)의 상관을, pH 및 활동성과 는 정(+)의 상관을 보였다. 특히, 활동성과 근내지방도간의 상관계수가 –0.65로 통계적 유의성을 보였는데 (p<0.05), 이러한 결과는 소의 활동성이 낮으면 근내지방도는 높아진다는 것을 제시하여 준다. 반면에 pH와 도체중 간의 상관계수는 0.50으로 나타나 정(+)의 상관을 보였다. 이는 반추위의 pH가 낮아지면 체중도 작아진다는 것으로 분석된다. 본 연구 결과를 확대하여 계절 및 시간대별 생체정보가 도축성적에 미치는 요인에 대하여 추가적인 분석이 필요하며, 이를 근거로 한우 생산성 향상을 위한 생체정보를 활용할 수 있는 정보를 제공해 줄 것으로 판단된다.

The decreased fertility is frequently thought to be problem of cattle production. However, studies figure out that number of these problems is related to bull factors especially in artificial insemination setting. Therefore, this study was designed to investigate the fertility status of bull by their estimated relative conception rate of cows that were inseminated by frozen semen from Korean proven bulls. Here we use the non-return rate (NRR) to access the bull fertility whereas, the NRR was define as the proportion of bulls that semen were used to inseminate cows and the number of cows that did not return for another service within 60 days. The data from 54,388 artificial inseminations (AI) were analyzed from 88 KPN semen. The NRRs of highest and lowest fertile bull were 83.81 and 51.33%, respectively. And mean NRR was 68.27%. In comparison to previously reported study, our data shows 17.38% higher NRR and the absolute value of difference in 50%>NRR and 50%<NRR group was 22.17 and 10.51, respectively (p< 0.001). In conclusion, the decreased fertility might consider as key aspect in achieving considerable conception of cows in existing integrated farming system at Korea.

Male factor infertility or sub-fertility contributed half of all cases of infertility while the semen abnormality is the current topic of argument. Conventional analysis of semen showed poor correlation with fertility. Therefore, evaluation of current semen analysis method is necessary to improve standards of semen assessment. The goal of this study was to investigate that correlation between motion kinematic before and after capacitation and litter size in porcine. Sperm motility and kinematics were measure by computer-assisted sperm analysis (CASA). The motility of spermatozoa was positively correlated with curvilinear velocity (VCL), average path velocity (VAP), and mean amplitude of head lateral displacement (ALH) (p<0.05). Where as VCL positively correlated with VSL, VAP and ALH (p<0.01). Straight-line velocity (VSL) was positively correlated with VAP and ALH (p<0.01). VAP was significantly positively correlated with ALH (p<0.01). Also, we found significant positive correlation among variation of VSL, VAP and ALH (p<0.05). No motility and kinematic parameter are correlated with litter size. However, litter size was significantly correlated with breed (p<0.05). Our results suggested that analysis of sperm motility and kinematics using CASA is questionable for prediction of litter size. However, it has some practical importance to evaluate semen commercially.

The objective of this study was to examine the effect of various discontinuous Percoll washing conditions on motile sperm recovery rate and motion kinematics. Frozen semen samples from 3 bulls (0.5 ml plastic straws, 6% glycerol in egg yolk-Tris-glycerol extender) were thawed in 37℃ water bath for 1 min. After thawing, the mixed semen samples were randomly allocated to 12 treatment groups. Briefly, the spermatozoa were centrifuged for three different time lengths (10, 20, and 30 min) at two gravities (300×g and 700×g) through two concentrations of discontinuous Percoll density gradient of 1 ml 90%: 1 ml 45% Percoll and 2 ml 90%: 2 ml 45% Percoll to remove extender, debris, and dead spermatozoa. Motile sperm recovery rate and motion kinematics were evaluated by computer assisted sperm analyzer using Makler counting chamber. Sperm motility (%) and motile sperm recovery rate showed similar pattern in all treatment groups. However, sperm motility (%) and motile sperm recovery rate were highest at 700×g for 30 min through a discontionous Percoll density gradient of 1 ml 90%: 1 ml 45% Percoll. There were no significant differences in motion kinematics after various Percoll washings. These results suggest that force of centrifugation, centrifugation time, and Percoll volume significantly affect motile sperm recovery rate.

The objective of this study was to investigate the effect of storage time on fresh boar semen in Androhep and Beltsville Thawing Solution (BTS). Boar semen samples extended in each extender were stored at 17℃ up to 4 days. Sperm motility kinematics was evaluated by computer assisted sperm analyzer (CASA) and capacitation status by chlortetracycline (CTC)/Hoechst 33258 staining. Sperm motility (%) was not decreased during storage in BTS and Androhep. No significant difference between extenders was observed. Only significant differences in kinematic parameters on linearity during storage were found. The percentage of dead sperm significantly decreased during storage (p<0.05). Also the percentage of noncapacitated, capacitated, and acrosome-reacted sperm significantly modified during storage (p<0.05). However, there was no significant difference between extenders except proportion of capacitated sperm. This finding supported that modification in these parameters was not significantly different between extenders during this short-term storage. Our finding strongly indicated that both Androhep and BTS maintained favorable conditions for motility, motility kinematics, and capacitation status during short-term storage. Despite modifications in some parameters were apparent during sperm storage in extenders, these may not affect the fertilizing capacity of boar semen.

지식경제부는 2009년부터 RFID(Radio-Frequency IDentification)와 USN(Ubiquitous Sensor Network) 기술의 중요성을 파악하고 이를 집중적으로 육성하여 2017년까지 세계 RFID/USN 산업에서 3강에 진입하겠다는 로드맵을 발표하였다. RFID와 USN은 전 세계적으로 매년 큰 폭으로 성장하고 있는 산업이며, 정부에서도 의욕적으로 지원을 발표하였다. 또한 RFID/USN 산업에 종사하고 있는 기업의 80%가 중소기업이기 때문에 이 분야에 대한 정부의 지원은 중소기업의 활성화라는 측면에서도 매우중요하다. 본 논문에서는 RFID/USN 최신 기술에 대해 간략하게 정리하였으며, 농림수산업분야에서 RFID/USN 기술을 적용한 연구 사례를 충실히 소개하고 있다. 또한 해외 사례를 토대로 RFID 및 USN 산업이 나아가야할 시사점을 제공함으로써, 정부가 유비쿼터스 분야의 커다란 로드맵을 구성하는데 있어 중요한 지표가 될 수 있을 것이다.

본 논문에서는 강상자형교의 바닥판과 주형에 대한 생애주기비용(Life-Cycle Cost : LCC)를 고려한 최적설계 방법을 제안하였다. 생애주기비용의 최적설계 문제는 초기비용, 유지관리비용 그리고 강도와 처짐 그리고 균열에 대한 파손 기대비용의 최소화 문제로 정식화할 수 있다. 기존의 재래적인 설계방법과의 비교를 동해서 강상자형교의 생애주기비용 최적설계의 우수성을 입증하였다. 또한 수치적인 결과의 고찰을 통하여 LCC에 근거한 최적설계가 여타의 설계방법들보다 좀 더 합리적이고 경제적이며 안전한 설계를 유도하는 것으로 분석되었다.