Human embryonic stem (hES) cells can be induced to differentiate into tyrosine hydroxylase expressing (TH+) cells that may serve as an alternative for cell replacement therapy for Parkinson's disease (PD). To examine in vitro differentiation of hES (MB03, registered in NIH) cells into TH+ cells, hES cells were induced to differentiate according to the 4-/4+ protocol using retinoic acid (RA), ascorbic acid (AA), and/or lithium chloride (LiCl) followed by culture in N2 medium for 14 days, during which time the differentiation occurs. Immunocytochemical stainings of the cells revealed that approximately 21.1% of cells treated with RA plus AA expressed TH protein that is higher than the ratio of TH+ cells seen in any other treatment groups (RA, RA+LiCl or RA+AA+LiCl). In order to see the differentiation pattern in vivo and the ability of in vitro differentiation-induced cells in easing symptomatic motor function of PD animal model, cells (2 10 cells/2) undergone 4-/4+ protocol using RA plus AA without any further treatment were transplanted into unilateral striatum of MPTP-lesioned PD animal model (C57BL/6). Following the surgery, motor behavior of the animals was examined by measuring the retention time on an accelerating rotar-rod far next 10 weeks. No significant differences in retention time of the animals were noticed until 2 weeks post-graft; however, it increased markedly at 6 weeks and 10 weeks time point after the surgery. Immunohistochemical studies confirmed that a reasonable number of TH+ cells were found at the graft site as well as other remote sites, showing the migrating nature of embryonic stem cells. These results suggest that in viかo differentiated hES cells relieve symptomatic motor behavior of PD animal model and should be considered as a promising alternative for the treatment of PD.

본 연구는 최근 우리나라 항만 물동량 증가세가 둔화되고 있는 가운데 국내 컨테이너터미널 운영기업의 경쟁력 강화를 위한 방안을 마련하고자 기업경쟁력 평가모형을 제시하고 글로벌 터미널 운영사와 비교분석하였다. 기업경쟁력 분석을 위한 퍼지모형은 퍼지이론과 AHP를 활용하였다. 경쟁력 평가를 위한 계층적 구조는 SERM 모형을 활용하였는바, 이는 기업의 성장이 주체(Subject), 환경(Environment), 자원(Resource), 그리고 메커니즘(Mechanism)의 상호작용 과정에서 형성된다는 논리에 바탕을 두고 있다. 분석결과 우리나라 컨테이너터미널 운영기업의 경쟁력은 글로벌 기업에 비해 전반적으로 약한 것으로 나타났다. 특히 기업의 대형화, 사업 다각화, 글로벌 전략수립이 시급한 것으로 나타났다.