Arthrobacter sp. L-3이 생성하는 glucose isomerase를 DEAE-cellulose column chromatography법으로 2단계 NaCl농도 구배로 용출함으로서 순수분리하였다. 이것이 SDS-acrylamide gel electrophoresis상에서 단일띠를 보임으로서 매우 잘 분리되었음을 알 수 있었다. Glucose isomerase의 K_m값과 V_max값이 각각 0.175M, 0.29(㎎/㎖/min)로 얻어졌다. 한편, SDS-acrylamide gel electrophoresis와 Sephadex-G100-50에 의한 gel filtration으로부터 분자량이 각각 42, 000과 180, 000으로 얻어져, 이 효소는 분자량이 42, 500인 4개의 subunit로 구성되었음을 알 수 있었다.
The morphological changes of fresh beef treated with ficin(0.1% : 2 hrs, 6 hrs)were examined with transmission electron microscope(TEM), the results obtained were as follows ; Connective tissue protein in fresh beef treated with ficin became gradually fragmentation and was occured stabilization with time. The length of sarcomere in myofibrillar protein was elongated, M-line became dim, and the I-band of Z-line was broken and became fragmentation with time.